breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_CP022686 | 162,427 | T→G | 7.0% | V53G (GTG→GGG) | ydgH → | DUF1471 family protein YdgH |
RA | NZ_CP022686 | 298,027 | A→C | 12.3% | F60C (TTT→TGT) | chbC ← | PTS N,N'‑diacetylchitobiose transporter subunit IIC |
RA | NZ_CP022686 | 360,227 | T→G | 19.3% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 372,574 | A→C | 12.7% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 496,086 | A→C | 14.1% | L399V (TTA→GTA) | otsA ← | alpha,alpha‑trehalose‑phosphate synthase |
RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
RA | NZ_CP022686 | 601,295 | T→G | 16.9% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,302 | T→G | 24.7% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,337 | T→G | 16.1% | V1621G (GTT→GGT) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
RA | NZ_CP022686 | 636,713 | A→C | 8.2% | R452R (CGT→CGG) | clbM ← | precolibactin export MATE transporter ClbM |
RA | NZ_CP022686 | 722,957 | A→C | 9.9% | N350T (AAC→ACC) | CIW80_RS03730 → | patatin‑like phospholipase family protein |
RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 764,997 | T→G | 12.7% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 829,435 | C→A | 18.8% | intergenic (‑24/+203) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,441 | C→A | 17.3% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,452 | G→T | 15.8% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 948,014 | C→G | 10.8% | intergenic (+83/‑174) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 1,040,909 | A→C | 10.7% | E232D (GAA→GAC) | rbn → | ribonuclease BN |
RA | NZ_CP022686 | 1,100,944 | T→G | 17.7% | D187A (GAC→GCC) | aroC ← | chorismate synthase |
RA | NZ_CP022686 | 1,174,272 | A→C | 15.2% | intergenic (+1/‑43) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
RA | NZ_CP022686 | 1,213,462 | A→C | 26.1% | L426R (CTG→CGG) | eutE ← | aldehyde dehydrogenase EutE |
RA | NZ_CP022686 | 1,239,833 | G→T | 15.9% | intergenic (‑130/+38) | ypfJ ← / ← purC | neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase |
RA | NZ_CP022686 | 1,280,117 | T→G | 10.3% | N8H (AAC→CAC) | yfgM ← | ancillary SecYEG translocon subunit YfgM |
RA | NZ_CP022686 | 1,366,277 | T→G | 6.9% | intergenic (‑278/+164) | CIW80_RS06740 ← / ← clpB | 16S ribosomal RNA/ATP‑dependent chaperone ClpB |
RA | NZ_CP022686 | 1,396,428 | T→G | 7.4% | intergenic (+34/‑203) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,479,585 | T→A | 11.3% | K3* (AAA→TAA) | ftsB ← | cell division protein FtsB |
RA | NZ_CP022686 | 1,479,591 | T→A | 9.9% | M1M (ATG→TTG) † | ftsB ← | cell division protein FtsB |
RA | NZ_CP022686 | 1,479,619 | T→A | 12.3% | intergenic (‑28/+165) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
RA | NZ_CP022686 | 1,504,258 | G→T | 12.8% | S232I (AGT→ATT) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,310 | G→T | 11.2% | M249I (ATG→ATT) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,547,003 | G→C | 15.8% | intergenic (+25/‑9) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,558,395 | A→C | 6.4% | K464N (AAA→AAC) | vgrG → | type VI secretion system Vgr family protein |
RA | NZ_CP022686 | 1,564,569 | A→C | 10.5% | K190N (AAA→AAC) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,632,501 | T→G | 10.7% | V166G (GTT→GGT) | arcC → | carbamate kinase |
RA | NZ_CP022686 | 1,633,846 | T→G | 30.5% | N253T (AAC→ACC) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,674,224 | T→G | 21.8% | K188N (AAA→AAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,244 | T→G | 18.4% | T182P (ACC→CCC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,248 | T→G | 9.7% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,716,145 | A→C | 11.3% | T42P (ACC→CCC) | rdgB → | XTP/dITP diphosphatase |
RA | NZ_CP022686 | 1,716,167 | A→C | 11.7% | N49T (AAC→ACC) | rdgB → | XTP/dITP diphosphatase |
RA | NZ_CP022686 | 1,911,618 | C→A | 6.5% | V219F (GTT→TTT) | CIW80_RS09520 ← | MurR/RpiR family transcriptional regulator |
RA | NZ_CP022686 | 1,933,952 | G→T | 5.2% | D127Y (GAT→TAT) | CIW80_RS09630 → | fimbrial biogenesis outer membrane usher protein |
RA | NZ_CP022686 | 2,027,390 | A→C | 7.8% | K80N (AAA→AAC) | agaE → | PTS N‑acetylgalactosamine transporter subunit IID |
RA | NZ_CP022686 | 2,049,089 | T→G | 19.3% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,110,457 | T→G | 8.8% | K12N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,110,465 | T→G | 8.5% | T10P (ACC→CCC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,148,115 | T→G | 15.6% | L126R (CTG→CGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,141 | T→G | 12.6% | F135V (TTC→GTC) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,176 | T→G | 7.2% | G146G (GGT→GGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,163,522 | C→A | 5.8% | intergenic (‑17/+23) | rrf ← / ← CIW80_RS10805 | 5S ribosomal RNA/tRNA‑Thr |
RA | NZ_CP022686 | 2,172,177 | A→C | 8.6% | G87G (GGT→GGG) | smg ← | DUF494 family protein Smg |
RA | NZ_CP022686 | 2,181,252 | G→T | 9.1% | Q136K (CAG→AAG) | rpsD ← | 30S ribosomal protein S4 |
RA | NZ_CP022686 | 2,185,075 | A→C | 8.7% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,272,797 | G→T | 12.0% | intergenic (+39/‑58) | greB → / → yhgF | transcription elongation factor GreB/Tex family protein |
RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,357,322 | T→A | 7.7% | S36R (AGT→AGA) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,362,972 | T→G | 7.8% | F158V (TTT→GTT) | nikB → | nickel ABC transporter permease subunit NikB |
RA | NZ_CP022686 | 2,394,287 | T→G | 24.5% | N115T (AAC→ACC) | chuS ← | hematinate‑forming heme oxygenase ChuS |
RA | NZ_CP022686 | 2,398,896 | A→C | 14.0% | K206N (AAA→AAC) | hutW → | heme anaerobic degradation radical SAM methyltransferase ChuW/HutW |
RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
RA | NZ_CP022686 | 2,433,335 | A→C | 11.4% | S1057A (TCG→GCG) | bcsC ← | cellulose synthase complex outer membrane protein BcsC |
JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
RA | NZ_CP022686 | 2,632,810 | T→A | 7.5% | I63F (ATT→TTT) | dgoT ← | MFS transporter |
RA | NZ_CP022686 | 2,719,166 | A→T | 6.8% | I122I (ATT→ATA) | ilvY ← | HTH‑type transcriptional activator IlvY |
RA | NZ_CP022686 | 2,736,578 | C→A | 7.2% | T180T (ACC→ACA) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,736,586 | A→C | 11.2% | Y183S (TAC→TCC) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,758,168 | A→C | 7.8% | N54H (AAC→CAC) | yifL → | lipoprotein |
RA | NZ_CP022686 | 2,777,201 | T→G | 5.6% | T255P (ACC→CCC) | metR ← | HTH‑type transcriptional regulator MetR |
RA | NZ_CP022686 | 2,780,110 | T→G | 7.1% | F677C (TTT→TGT) | metE → | 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase |
RA | NZ_CP022686 | 2,821,644 | A→C | 7.6% | intergenic (+100/‑278) | hemG → / → CIW80_RS14130 | menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA |
RA | NZ_CP022686 | 2,852,911 | T→G | 9.0% | G172G (GGT→GGG) | CIW80_RS14270 → | aldolase |
RA | NZ_CP022686 | 2,868,740 | T→G | 26.0% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,868,753 | T→G | 11.7% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,920,385 | C→A | 5.5% | A54E (GCA→GAA) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
RA | NZ_CP022686 | 2,981,597 | G→T | 12.8% | E562* (GAA→TAA) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
RA | NZ_CP022686 | 3,020,229 | T→G | 9.7% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
RA | NZ_CP022686 | 3,045,253 | C→A | 5.6% | I288I (ATC→ATA) | lamB → | maltoporin LamB |
RA | NZ_CP022686 | 3,096,743 | A→C | 11.4% | intergenic (+29/+7) | yjcE → / ← CIW80_RS15405 | Na+/H+ antiporter/LysR family transcriptional regulator |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,213,819 | T→G | 7.1% | R479R (CGT→CGG) | nnr → | bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase |
RA | NZ_CP022686 | 3,252,190 | T→G | 9.5% | K221N (AAA→AAC) | CIW80_RS16225 ← | enoyl‑CoA hydratase/isomerase family protein |
RA | NZ_CP022686 | 3,265,084 | T→G | 18.5% | N77T (AAC→ACC) | ytfJ ← | YtfJ family protein |
RA | NZ_CP022686 | 3,348,342 | A→T | 11.1% | intergenic (‑158/‑42) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,348,359 | A→T | 7.2% | intergenic (‑175/‑25) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,348,371 | T→G | 10.5% | intergenic (‑187/‑13) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
RA | NZ_CP022686 | 3,443,420 | T→G | 5.1% | D153A (GAC→GCC) | yjiA ← | GTPase |
RA | NZ_CP022686 | 3,461,200 | C→A | 12.6% | intergenic (‑9/+26) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
RA | NZ_CP022686 | 3,461,215 | A→T | 14.2% | intergenic (‑24/+11) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
RA | NZ_CP022686 | 3,461,229 | T→G | 22.2% | noncoding (84/87 nt) | CIW80_RS17340 ← | tRNA‑Leu |
RA | NZ_CP022686 | 3,461,363 | A→C | 13.2% | noncoding (65/87 nt) | CIW80_RS17345 ← | tRNA‑Leu |
JC | NZ_CP022686 | 3,509,100 | Δ12 bp | 46.5% | coding (1567‑1578/1917 nt) | dnaK → | molecular chaperone DnaK |
RA | NZ_CP022686 | 3,652,079 | T→G | 7.6% | intergenic (‑88/+24) | panB ← / ← CIW80_RS18255 | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin |
RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,660,810 | C→A | 31.3% | R245L (CGC→CTC) | pcnB ← | polynucleotide adenylyltransferase PcnB |
RA | NZ_CP022686 | 3,665,907 | A→C | 6.4% | Q462H (CAA→CAC) | hrpB → | ATP‑dependent helicase HrpB |
RA | NZ_CP022686 | 3,734,367 | T→G | 15.1% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,770,508 | T→G | 7.5% | intergenic (‑244/+219) | CIW80_RS26265 ← / ← CIW80_RS18890 | ATP‑binding protein/hypothetical protein |
RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
RA | NZ_CP022686 | 3,797,481 | T→G | 17.4% | L130V (TTA→GTA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,800,981 | A→C | 8.8% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
RA | NZ_CP022686 | 3,824,405 | T→G | 8.7% | S242A (TCT→GCT) | CIW80_RS19155 → | TolC family protein |
RA | NZ_CP022686 | 3,852,459 | T→G | 11.0% | N178T (AAC→ACC) | phoE ← | phosphoporin PhoE |
RA | NZ_CP022686 | 3,868,846 | T→G | 10.3% | T383P (ACG→CCG) | ecpC ← | fimbrial usher EcpC |
RA | NZ_CP022686 | 3,925,289 | A→C | 16.8% | N156T (AAC→ACC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,928,345 | T→G | 14.9% | F277V (TTC→GTC) | prpE → | propionate‑‑CoA ligase |
RA | NZ_CP022686 | 3,928,356 | T→G | 8.7% | A280A (GCT→GCG) | prpE → | propionate‑‑CoA ligase |
RA | NZ_CP022686 | 3,935,324 | T→G | 12.4% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,981,933 | T→G | 13.6% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
RA | NZ_CP022686 | 4,056,962 | C→A | 15.0% | F137L (TTC→TTA) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,067,840 | A→C | 6.7% | K7N (AAA→AAC) | ybaQ → | HigA family addiction module antitoxin |
RA | NZ_CP022686 | 4,079,344 | C→A | 11.8% | A165S (GCG→TCG) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
RA | NZ_CP022686 | 4,079,363 | A→C | 8.6% | A158A (GCT→GCG) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
RA | NZ_CP022686 | 4,118,545 | G→T | 8.0% | S252* (TCA→TAA) | nfrA ← | bacteriophage adsorption protein NfrA |
RA | NZ_CP022686 | 4,280,095 | T→G | 9.4% | G103G (GGT→GGG) | cydB → | cytochrome d ubiquinol oxidase subunit II |
RA | NZ_CP022686 | 4,287,450 | A→C | 10.4% | noncoding (41/76 nt) | CIW80_RS21475 → | tRNA‑Val |
RA | NZ_CP022686 | 4,331,897 | G→T | 16.6% | S303* (TCG→TAG) | ybhR ← | ABC transporter permease |
RA | NZ_CP022686 | 4,368,372 | T→G | 14.6% | S37R (AGC→CGC) | ybiY ← | glycyl‑radical enzyme activating protein |
RA | NZ_CP022686 | 4,368,380 | T→G | 10.4% | N34T (AAC→ACC) | ybiY ← | glycyl‑radical enzyme activating protein |
RA | NZ_CP022686 | 4,412,284 | A→C | 16.7% | W241G (TGG→GGG) | ybjT ← | SDR family oxidoreductase |
RA | NZ_CP022686 | 4,535,349 | A→C | 12.9% | T118T (ACA→ACC) | appA → | bifunctional acid phosphatase/4‑phytase |
RA | NZ_CP022686 | 4,587,091 | T→G | 8.8% | N609T (AAC→ACC) | CIW80_RS22915 ← | enterotoxin |
RA | NZ_CP022686 | 4,678,047 | A→C | 40.3% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,712,737 | A→T | 13.8% | L61Q (CTG→CAG) | yceB ← | lipoprotein |
RA | NZ_CP022686 | 4,764,877 | T→G | 16.9% | intergenic (‑57/‑204) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,764,896 | C→G | 13.0% | intergenic (‑76/‑185) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,764,904 | G→T | 10.3% | intergenic (‑84/‑177) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,780,176 | A→C | 15.6% | intergenic (‑75/+95) | phoP ← / ← purB | two‑component system response regulator PhoP/adenylosuccinate lyase |
RA | NZ_CP022686 | 4,815,879 | T→G | 11.3% | F23V (TTC→GTC) | CIW80_RS24305 → | C40 family peptidase |
RA | NZ_CP022686 | 4,821,347 | A→C | 8.7% | K207N (AAA→AAC) | CIW80_RS24320 → | prophage tail fiber N‑terminal domain‑containing protein |
RA | NZ_CP022686 | 4,839,687 | T→G | 14.7% | N144T (AAC→ACC) | minD ← | septum site‑determining protein MinD |
RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | NZ_CP022686 | 4,905,220 | C→G | 13.2% | L116V (CTT→GTT) ‡ | CIW80_RS24815 → | coenzyme F390 synthetase |
RA | NZ_CP022686 | 4,905,221 | T→G | 15.2% | L116R (CTT→CGT) ‡ | CIW80_RS24815 → | coenzyme F390 synthetase |
RA | NZ_CP022686 | 4,951,521 | A→C | 9.3% | T39P (ACC→CCC) | topA → | type I DNA topoisomerase |
RA | NZ_CP022686 | 5,042,304 | T→C | 9.8% | N645N (AAT→AAC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,044,182 | T→A | 7.6% | G1271G (GGT→GGA) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,044,641 | C→T | 5.0% | N1424N (AAC→AAT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,465 | +GGCTAGCTCA | 11.8% | intergenic (‑1669/‑66) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] | |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,623:1 | +T | 9.5% | coding (15/399 nt) | IL2wt no signal → |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 303–2618 | 3084–2957 | 340–2782 | 8790 [8639] | [8415] 8850 | [JNG15_RS00005]–mobA | [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4753–4810 | 5478–5073 | 264–726 | 3803 [3611] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | = 1524130 | NA (NA) | 45 (0.010) | 27/248 | NT | 5.7% | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3633 = | 749 (0.120) | coding (25/399 nt) | IL2wt no signal | ||||||
* | ? | NZ_CP022686 | = 1761699 | NA (NA) | 18 (0.010) +24 bp |
15/200 | NT | 84.8% | intergenic (‑74/‑68) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3594 | 4 (0.000) | intergenic (+1/‑15) | ompA/IL2wt no signal | –/– | |||||
* | ? | NZ_CP022686 | 3393988 = | NA (NA) | 45 (0.020) +23 bp |
24/202 | NT | 93.3% | intergenic (‑51/+11) | CIW80_RS16975/CIW80_RS16980 | hypothetical protein/IS3‑like element ISEc52 family transposase |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3594 | 4 (0.000) | intergenic (+1/‑15) | ompA/IL2wt no signal | –/– | |||||
* | ? | NZ_CP022686 | 3899666 = | 154 (0.850) | 66 (0.420) | 35/216 | NT | 36.0% | intergenic (‑40/+290) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | 3899944 = | 101 (0.640) | intergenic (‑318/+12) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
* | ? | NZ_CP022686 | = 3899681 | 153 (0.840) | 59 (0.370) | 35/216 | NT | 33.5% | intergenic (‑55/+275) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | = 3899927 | 101 (0.640) | intergenic (‑301/+29) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
* | ? | NZ_CP022686 | 4514374 = | 155 (0.860) | 7 (0.000) +AGCT |
6/240 | NT | 8.3% | coding (59/1053 nt) | ompA | porin OmpA |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3609 = | 4 (0.000) | coding (1/399 nt) | IL2wt no signal |