breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 340,516 A→C 11.0% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 13.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 15.9% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 20.4% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,274 A→C 12.9% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 418,374 T→G 11.5% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 436,591 A→C 8.5% intergenic (‑19/+38) CIW80_RS02305 ← / ← CIW80_RS02310 phage minor tail protein L/phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 481,005 A→C 13.1% L620R (CTG→CGG)  flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 491,004 T→G 6.6% T256P (ACG→CCG)  cheA ← chemotaxis protein CheA
RA NZ_CP022686 496,135 A→C 9.3% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 531,408 A→C 15.2% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,940 A→T 11.8% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 18.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,757 C→A 11.3% G438W (GGG→TGG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,848 A→C 14.1% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 828,846 A→C 9.1% L189R (CTT→CGT)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA NZ_CP022686 829,441 C→A 20.6% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 18.7% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 17.7% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.2% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 864,173 T→G 18.3% intergenic (+39/+2) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 878,883 T→G 11.5% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 881,742 A→C 17.8% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 20.0% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,049 A→T 10.8% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 10.5% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,005,071 G→C 7.3% E376Q (GAA→CAA)  nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,082 T→A 13.4% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 11.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,174,272 A→C 16.8% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 18.5% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,456 A→C 26.7% L428R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,213,462 A→C 31.5% L426R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,293,976 G→T 9.1% E212* (GAA→TAA)  sseA → 3‑mercaptopyruvate sulfurtransferase
RA NZ_CP022686 1,317,580 T→G 6.8% T126P (ACC→CCC)  yphG ← DUF5107 domain‑containing protein
RA NZ_CP022686 1,360,724 G→T 13.8% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 9.6% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,396,420 T→G 7.3% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,460,615 A→C 11.1% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 7.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,625 G→C 6.5% intergenic (‑34/+159) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,634 G→C 11.1% intergenic (‑43/+150) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,355 T→A 9.2% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 16.3% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 11.7% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 6.9% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,573 T→G 9.0% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,217 A→C 12.8% T740P (ACG→CCG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,579,312 A→C 7.5% V107V (GTT→GTG CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,620,788 C→A 5.8% intergenic (‑353/‑61) actS ← / → xdhA amidase activator ActS/xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA NZ_CP022686 1,640,925 A→C 12.1% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 13.7% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 7.5% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 6.6% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 9.0% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,727,088 A→C 6.5% V441G (GTT→GGT)  speC ← ornithine decarboxylase
RA NZ_CP022686 1,744,260 G→T 6.1% P327P (CCG→CCT CIW80_RS08650 → IS3 family transposase
RA NZ_CP022686 1,925,332 A→C 11.2% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 20.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,173 A→C 12.7% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,170 A→C 6.0% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 11.9% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 12.5% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,193 A→C 6.2% V606G (GTT→GGT)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,552 A→C 10.4% intergenic (+22/‑211) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,554 T→G 13.0% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 8.1% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,475 A→T 6.0% A6A (GCT→GCA rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 6.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 11.6% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,179,916 G→T 9.5% R22R (CGC→CGA rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 10.4% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,194,054 G→T 8.0% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,194,081 A→C 7.2% L435V (TTG→GTG)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,207,089 C→A 6.9% intergenic (‑113/+56) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 7.7% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,272,797 G→T 14.0% intergenic (+39/‑58) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,272,810 T→G 11.3% intergenic (+52/‑45) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 11.4% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 16.3% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,972 T→G 5.1% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,394,295 G→T 8.8% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,302 A→T 14.6% F110Y (TTC→TAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 11.9% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,535 A→C 7.8% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 20.6% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,216 T→G 7.7% S542S (TCT→TCG upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,312 T→G 12.7% G574G (GGT→GGG upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,873 A→C 6.1% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,521,024 T→G 7.8% V229G (GTT→GGT)  lldP → L‑lactate permease
RA NZ_CP022686 2,525,668 A→C 5.8% R262R (CGT→CGG gpsA ← NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA NZ_CP022686 2,534,743 C→A 8.0% D201Y (GAT→TAT)  kbl ← glycine C‑acetyltransferase
RA NZ_CP022686 2,546,394 A→C 12.6% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 18.6% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,572,705 A→C 11.9% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 11.0% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 10.5% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,640,661 C→A 8.5% E227* (GAA→TAA)  gyrB ← DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,643,657 A→C 9.9% G431G (GGT→GGG dnaA ← chromosomal replication initiator protein DnaA
RA NZ_CP022686 2,692,903 G→T 7.0% E358* (GAG→TAG)  kup → low affinity potassium transporter Kup
RA NZ_CP022686 2,703,299 A→C 8.7% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,743,563 T→G 11.5% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,109 A→C 8.5% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 8.4% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,769,196 A→C 16.3% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,864 T→G 5.9% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,114 A→T 7.9% I437F (ATT→TTT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,808,132 A→T 6.5% I443F (ATT→TTT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,808,145 T→G 6.9% V447G (GTG→GGG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,810,040 A→C 5.4% N242T (AAC→ACC)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,821,644 A→C 7.8% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 7.2% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 21.4% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 11.8% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 9.0% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 14.9% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 8.7% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,920,385 C→A 5.9% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,961,318 A→C 7.4% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,625 A→C 7.4% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 9.4% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,229 T→G 8.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,114,640 A→C 7.5% G40G (GGT→GGG CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,136,940 T→G 14.6% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,252 T→G 8.6% F20V (TTT→GTT)  rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,150,950 A→C 5.4% Q252H (CAA→CAC rdcB → YjcZ‑like family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,252,190 T→G 13.3% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,252,198 C→A 19.4% E219* (GAA→TAA)  CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,281,059 T→G 9.9% L93R (CTG→CGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,360 T→G 8.1% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 7.4% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 7.1% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,605 T→G 15.1% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,614 G→T 6.9% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,834 C→A 11.7% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,221 T→G 15.4% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 25.0% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,496,444 T→G 10.4% I270S (ATC→AGC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,496,461 T→G 9.6% F276V (TTT→GTT)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,504,054 T→G 7.7% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,547,903 T→G 7.9% F15V (TTT→GTT)  CIW80_RS17770 → CcdB family protein
RA NZ_CP022686 3,634,614 A→C 9.7% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 8.7% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 8.8% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,660,415 G→A 10.9% Q377* (CAG→TAG)  pcnB ← polynucleotide adenylyltransferase PcnB
JC NZ_CP022686 3,660,469 Δ39 bp 14.4% coding (1037‑1075/1398 nt) pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,661,221 A→G 8.6% L108P (CTG→CCG)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,713,182 A→C 10.5% E39D (GAA→GAC yaeR → VOC family protein
RA NZ_CP022686 3,734,367 T→G 20.0% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 15.1% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,800,979 A→C 7.3% pseudogene (1255/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,256 G→T 16.7% E1583* (GAA→TAA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,868,846 T→G 12.7% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,908 A→C 6.0% L362R (CTG→CGG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,457 A→C 9.0% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,885,473 T→A 8.6% D1409E (GAT→GAA fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,925,318 G→C 9.1% D166H (GAC→CAC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 12.2% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 9.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 8.9% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 8.2% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,238 C→G 10.0% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 10.3% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 17.7% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,073,132 C→A 10.6% S404R (AGC→AGA ybaT → amino acid permease
RA NZ_CP022686 4,079,329 A→C 7.1% S170A (TCA→GCA)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,079,332 A→C 7.8% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,213,260 T→G 8.2% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,347 C→A 9.7% intergenic (‑10/+6) CIW80_RS21090 ← / ← CIW80_RS21095 tRNA‑Met/tRNA‑Gln
RA NZ_CP022686 4,233,084 G→A 6.9% S150N (AGC→AAC)  glnS → glutamine‑‑tRNA ligase
RA NZ_CP022686 4,286,455 T→G 7.3% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,290,599 T→G 7.3% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,301,920 T→G 8.4% V136G (GTT→GGT)  modB → molybdate ABC transporter permease subunit
RA NZ_CP022686 4,331,905 A→C 8.8% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,338,890 A→C 11.3% Q441H (CAA→CAC rhlE → ATP‑dependent RNA helicase RhlE
RA NZ_CP022686 4,361,126 C→A 6.1% S351* (TCG→TAG)  ybiR → anion transporter
RA NZ_CP022686 4,368,341 T→G 13.1% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 10.4% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,474,091 T→G 8.7% L264V (TTG→GTG)  ycbJ → YcbJ family phosphotransferase
RA NZ_CP022686 4,485,614 T→G 9.6% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,301 T→G 11.8% P102P (CCT→CCG appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,535,349 A→C 12.8% T118T (ACA→ACC appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,605,579 T→G 15.9% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,615,712 G→T 49.4% G122C (GGT→TGT)  CIW80_RS23065 → hypothetical protein
RA NZ_CP022686 4,632,182 T→G 8.2% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,189 T→G 7.1% S279A (TCC→GCC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,041 A→C 20.8% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 24.5% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 18.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 10.7% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,839,687 T→G 15.9% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,909,241 A→T 7.7% intergenic (‑7/+27) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,951,521 A→C 8.5% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,041,188 C→T 6.5% S273S (AGC→AGT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,041,191 G→A 5.7% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 8.2% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 6.7% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,561:1 +C 6.7% coding (31/63 nt) ompA →
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 16.9% E52* (GAA→TAA)  IL2wt no signal →
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 6.7% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 380–2618 3063–2957 340–2684 4280 [3939] [4191] 4264 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4740–4810 5417–5214 405–678 3099 [2918] [2768] 3175 [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1522881 =NA (NA)571 (0.240) 112/250 NT 17.7% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3495 = 2648 (0.590)intergenic (‑1699/‑36) putative rom (RNA I inhibition modulator protein)/ompA –/–
* ? NZ_CP022686 1522881 =NA (NA)5 (0.000) 4/250 NT 71.4% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3610 = 2 (0.000)coding (2/399 nt) IL2wt no signal
* ? NZ_CP022686 1522881 =NA (NA)5 (0.000) 4/250 NT 71.4% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3611 = 2 (0.000)coding (3/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1524130NA (NA)911 (0.380) 136/250 NT 26.1% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3497 2583 (0.570)intergenic (‑1701/‑34) putative rom (RNA I inhibition modulator protein)/ompA –/–
* ? NZ_CP022686 3899666 =139 (0.660)102 (0.550) 47/218 NT 46.7% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 112 (0.610)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681132 (0.620)135 (0.730) 69/218 NT 54.3% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 112 (0.610)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA