breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 133,849 T→G 5.0% T465P (ACG→CCG)  ydfI ← mannitol dehydrogenase family protein
RA NZ_CP022686 162,405 T→G 7.5% F46V (TTT→GTT)  ydgH → DUF1471 family protein YdgH
RA NZ_CP022686 196,840 A→C 5.9% N132T (AAC→ACC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 298,027 A→C 10.0% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,106 A→C 12.3% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 355,148 T→G 5.1% G139G (GGT→GGG
D270A (GAC→GCC) 
yeaL →
nimR ←
DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR
RA NZ_CP022686 360,227 T→G 20.9% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 11.9% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 402,874 A→C 8.2% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 443,269 C→A 5.3% Q671H (CAG→CAT CIW80_RS02355 ← Clp protease ClpP
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 597,830 A→C 11.4% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 14.4% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 23.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,757 C→A 11.7% G438W (GGG→TGG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,817 G→T 12.6% P249T (CCA→ACA)  clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 651,848 A→C 12.9% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,001 A→C 5.8% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,402 G→T 10.1% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,421 G→T 10.0% intergenic (+181/‑15) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,435 C→A 5.8% intergenic (+195/‑1) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 759,026 T→G 16.5% K223N (AAA→AAC CIW80_RS03960 ← glycosyltransferase family 2 protein
RA NZ_CP022686 759,039 A→T 17.4% L219* (TTA→TAA)  CIW80_RS03960 ← glycosyltransferase family 2 protein
RA NZ_CP022686 764,997 T→G 12.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 777,349 C→A 9.2% M221I (ATG→ATT gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 26.0% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 22.7% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 17.1% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 15.0% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,561 G→T 8.8% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,589 A→T 9.7% intergenic (‑162/+61) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 13.4% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 881,733 A→C 15.4% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 15.5% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 9.7% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 13.9% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 25.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 957,147 T→G 6.4% N77T (AAC→ACC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 998,355 A→C 6.2% V48G (GTA→GGA)  gyrA ← DNA topoisomerase (ATP‑hydrolyzing) subunit A
RA NZ_CP022686 1,005,079 G→T 6.3% L378L (CTG→CTT nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 13.6% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,065,978 A→C 6.6% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,907 T→G 15.1% E199D (GAA→GAC aroC ← chorismate synthase
RA NZ_CP022686 1,100,959 T→G 13.1% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,172,467 C→A 15.2% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,174,279 A→T 8.6% intergenic (+8/‑36) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,203,715 T→G 6.9% G238G (GGT→GGG hemF → oxygen‑dependent coproporphyrinogen oxidase
RA NZ_CP022686 1,213,479 T→G 13.2% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,213,483 T→G 14.7% N419T (AAC→ACC)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,918 C→A 9.4% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,280,117 T→G 6.6% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,709 T→G 11.2% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 9.8% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,739 A→T 8.4% intergenic (‑74/+250) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 10.7% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 16.0% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,619 T→G 8.3% E78D (GAA→GAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,071 G→T 12.6% D29Y (GAT→TAT)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,081 C→A 18.9% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,424,327 T→A 8.6% intergenic (‑23/+40) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,424,362 A→T 7.5% intergenic (‑58/+5) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,479,595 G→C 9.3% intergenic (‑4/+189) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,607 A→C 12.4% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 14.9% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,373 G→C 9.9% K270N (AAG→AAC CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 20.7% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,113 G→C 21.0% intergenic (+25/‑9) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,569 A→C 11.2% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 7.6% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,640,888 G→T 10.1% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,640,925 A→C 14.3% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 13.5% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 16.2% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.2% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 11.9% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,011 G→T 8.8% intergenic (‑621/‑34) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 8.8% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 13.5% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,716,180 A→C 11.9% K53N (AAA→AAC rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,202 A→C 11.1% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 7.8% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,860,060 T→G 18.1% K122T (AAA→ACA)  CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,860,070 C→A 18.7% E119* (GAA→TAA)  CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,865,103 A→C 7.6% L491V (TTG→GTG)  pitB ← inorganic phosphate transporter PitB
RA NZ_CP022686 1,911,602 T→G 10.7% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 2,016,160 T→G 9.8% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 8.6% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 21.2% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 9.9% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,799 G→T 11.5% E390* (GAA→TAA)  argG → argininosuccinate synthase
RA NZ_CP022686 2,061,804 A→C 9.3% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,076,552 A→C 9.8% intergenic (+22/‑211) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,554 T→G 12.7% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,556 C→G 6.8% intergenic (+26/‑207) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 13.3% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,234 T→A 6.5% Y210F (TAT→TTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,123,241 A→C 11.4% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,115 T→G 11.2% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,143 C→G 10.2% F135L (TTC→TTG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,179,878 T→G 13.5% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,071 A→C 8.7% G47G (GGT→GGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 8.5% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 6.9% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,207,100 C→A 8.7% intergenic (‑124/+45) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,209,101 A→T 5.3% I249I (ATT→ATA chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 7.3% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 14.1% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,954 T→G 6.5% F152V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,362,972 T→G 9.3% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,393,013 T→G 6.9% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 14.1% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,295 G→T 8.0% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 14.8% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 12.7% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,558,864 T→G 7.4% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,721 A→C 8.2% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 9.6% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 12.4% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,718,627 T→G 8.8% F510V (TTC→GTC)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,585 T→A 11.5% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 15.3% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 11.6% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 6.9% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 8.1% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,868,740 T→G 19.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 14.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.4% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 10.7% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 6.5% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 7.6% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,418 C→A 6.2% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,961,318 A→C 8.2% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,989,627 T→G 8.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,042,379 T→G 5.9% T154P (ACC→CCC)  malE ← maltose/maltodextrin ABC transporter substrate‑binding protein MalE
RA NZ_CP022686 3,086,834 A→C 10.0% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,124,925 A→C 5.5% F215V (TTT→GTT)  mdtN ← multidrug efflux transporter periplasmic adaptor subunit MdtN
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,281,004 T→G 10.3% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,062 T→G 7.2% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,374 C→G 6.9% intergenic (‑190/‑10) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,616 G→T 7.7% I231I (ATC→ATA sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,387,650 A→C 6.4% V220G (GTC→GGC)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,614 G→T 10.3% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,671 T→G 9.1% K124N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,459,376 C→A 10.9% S64Y (TCT→TAT)  bglJ → DNA‑binding transcriptional activator BglJ
RA NZ_CP022686 3,461,127 A→C 7.4% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,200 C→A 11.7% intergenic (‑9/+26) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 12.0% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 10.0% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 19.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 6.6% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 10.9% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,493,172 G→T 10.5% W449C (TGG→TGT creD → cell envelope integrity protein CreD
RA NZ_CP022686 3,496,452 A→C 12.2% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,516,075 T→G 8.5% V213G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,547,903 T→G 7.6% F15V (TTT→GTT)  CIW80_RS17770 → CcdB family protein
RA NZ_CP022686 3,604,564 G→T 10.0% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,606,005 G→T 7.1% V239V (GTG→GTT secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,634,614 A→C 11.6% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,655,540 A→C 8.8% F765V (TTT→GTT)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,667,058 A→C 7.6% D5A (GAC→GCC)  mrcB → bifunctional glycosyl transferase/transpeptidase
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 26.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 21.5% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,521 C→G 5.5% intergenic (‑257/+206) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,796,795 A→C 7.0% V10G (GTC→GGC)  CIW80_RS19060 ← PTS sugar transporter subunit IIA
RA NZ_CP022686 3,806,265 T→A 9.7% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,824,318 C→G 8.8% L213V (CTT→GTT)  CIW80_RS19155 → TolC family protein
RA NZ_CP022686 3,850,144 C→A 5.6% intergenic (+42/‑50) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,868,846 T→G 10.7% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,289 A→C 10.1% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 10.7% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,355 C→G 13.8% A280G (GCT→GGT)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,376 T→G 9.8% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 8.2% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,656 A→C 9.9% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 12.7% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,225 C→G 7.9% intergenic (+71/‑273) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,238 C→G 11.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 10.9% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,247 G→T 8.8% intergenic (+93/‑251) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 9.4% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,946 T→G 10.0% V132G (GTT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,056,951 C→A 15.2% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 19.2% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,067,840 A→C 6.0% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,073,132 C→A 14.7% S404R (AGC→AGA ybaT → amino acid permease
RA NZ_CP022686 4,133,850 T→G 11.7% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,196,276 T→G 12.1% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,213,266 A→T 6.8% L190F (TTA→TTT ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,126 T→G 9.2% intergenic (‑26/+12) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,280,095 T→G 8.7% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,286,455 T→G 7.5% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,290,599 T→G 6.7% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,335,504 T→G 5.6% K58N (AAA→AAC ybhF ← ATP‑binding cassette domain‑containing protein
RA NZ_CP022686 4,368,341 T→G 17.5% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 9.4% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 15.5% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 19.2% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,732 T→G 7.8% intergenic (+86/‑84) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,485,614 T→G 8.8% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,330 A→C 7.8% D112A (GAC→GCC)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,547,558 A→C 6.2% T144P (ACC→CCC)  torD → molecular chaperone TorD
RA NZ_CP022686 4,562,561 C→A 10.2% R136L (CGT→CTT)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,605,579 T→G 16.8% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 12.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 9.7% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,661,459 T→G 6.3% pseudogene (559/655 nt) CIW80_RS26570 → hypothetical protein
RA NZ_CP022686 4,712,763 A→C 12.8% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 11.7% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 7.2% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 7.4% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 12.9% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,791,863 C→A 8.6% T80T (ACC→ACA CIW80_RS24115 → CII family transcriptional regulator
RA NZ_CP022686 4,809,441 T→G 8.4% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,839,687 T→G 12.9% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,855,513 G→C 5.9% S139C (TCT→TGT)  cvrA ← potassium/proton antiporter
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,163 C→A 8.2% P97T (CCC→ACC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,951,521 A→C 7.6% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 11.0% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,000,015 T→G 7.2% F156V (TTT→GTT)  CIW80_RS25310 → ABC transporter ATP‑binding protein
RA NZ_CP022686 5,001,164 A→C 13.7% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,041,191 G→A 5.7% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 9.4% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→G 13.4% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 8.0% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 6.5% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 590–2618 3084–2957 340–2495 8625 [8552] [8504] 9188 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4740–4809 5478–5214 406–739 4556 [4335] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3743172 =270 (1.230)22 (0.100) 13/248 NT 7.9% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 244 (1.120)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase
* ? NZ_CP022686 3899666 =230 (1.050)41 (0.210) 28/218 NT 17.7% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 182 (0.950)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681223 (1.020)52 (0.270) 35/218 NT 21.7% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 182 (0.950)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 4514374 =223 (1.020)7 (0.000)
+AGCT
5/242 NT 5.9% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 6 (0.000)coding (1/399 nt) IL2wt no signal