breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 123,114 T→G 5.7% N354T (AAC→ACC)  CIW80_RS00630 ← PTS sugar transporter subunit IIC
RA NZ_CP022686 192,476 T→A 6.4% R629R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 263,831 A→C 10.5% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 10.8% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 360,227 T→G 18.5% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,290 T→G 8.8% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,958 T→A 11.5% intergenic (+142/‑706) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 16.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 18.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 693,021 A→C 8.2% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 14.2% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,014 A→T 14.3% V35V (GTT→GTA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 21.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.7% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 12.5% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 13.4% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 957,112 T→G 10.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,407 T→G 13.0% Q953P (CAG→CCG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 7.3% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,082 T→A 8.0% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,065,997 A→C 10.4% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,172,467 C→A 11.7% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,172,515 G→T 7.3% intergenic (+44/+7) yfeD → / ← gltX putative DNA‑binding transcriptional regulator/glutamate‑‑tRNA ligase
RA NZ_CP022686 1,174,306 T→A 7.5% intergenic (+35/‑9) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,935 A→C 17.5% V387G (GTG→GGG)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,237,918 C→A 10.0% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,239,833 G→T 16.6% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,360,724 G→T 12.7% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 9.6% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 9.8% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,579 C→A 18.6% D92Y (GAT→TAT)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,460,615 A→C 13.0% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 16.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 13.5% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 18.1% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,000 G→C 10.9% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 24.0% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,566,217 A→C 10.7% T740P (ACG→CCG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 11.5% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,632,525 T→G 7.8% V174G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,640,925 A→C 13.7% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 22.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 14.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.7% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,710,453 A→C 7.9% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,817,059 G→T 13.5% P332H (CCT→CAT)  CIW80_RS09065 ← K5 polysaccharide biosynthesis glycosyltransferase KfiC
RA NZ_CP022686 1,954,923 T→G 20.8% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,974,549 T→G 5.5% V986G (GTC→GGC)  ebgA → beta‑galactosidase subunit alpha
RA NZ_CP022686 2,016,225 T→G 10.1% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 15.0% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 8.1% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,780 A→C 5.9% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,457 T→G 12.2% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,128,043 T→G 7.7% D438A (GAC→GCC)  rng ← ribonuclease G
RA NZ_CP022686 2,148,109 T→G 12.5% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 13.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,126 A→T 9.5% S130C (AGT→TGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 11.3% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,184,330 G→T 15.3% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 10.5% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,248 A→C 8.1% intergenic (‑117/+48) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,192,062 A→C 9.4% R69R (CGT→CGG rplD ← 50S ribosomal protein L4
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,213 G→C 5.6% F38L (TTC→TTG tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,272,831 G→T 6.9% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,473 A→C 6.8% D374E (GAT→GAG CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,173 T→G 6.9% L108V (TTG→GTG)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,361,489 T→G 16.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,380,922 A→C 11.4% S182R (AGC→CGC)  pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 9.2% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,398,896 A→C 10.7% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,470,422 T→G 10.3% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,873 A→C 7.8% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,705 A→C 11.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,717,400 T→G 7.9% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,758,128 A→C 8.2% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,140 G→C 5.7% T44T (ACG→ACC yifL → lipoprotein
RA NZ_CP022686 2,769,155 A→C 6.6% S61A (TCC→GCC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,186 C→A 11.1% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 19.0% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,868,740 T→G 21.4% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 8.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.4% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 11.8% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 8.5% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 9.0% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,218 C→A 8.2% E216* (GAA→TAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,937,239 T→G 7.5% T209P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,992,831 A→C 9.2% K133T (AAA→ACA)  nfi → deoxyribonuclease V
RA NZ_CP022686 2,994,757 C→A 6.3% S183* (TCG→TAG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,013,752 T→G 10.1% V471G (GTG→GGG)  aceK → bifunctional isocitrate dehydrogenase kinase/phosphatase
RA NZ_CP022686 3,136,940 T→G 12.7% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,953 T→G 8.2% T157P (ACG→CCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,142,423 A→C 8.5% L55R (CTG→CGG)  phnH ← phosphonate C‑P lyase system protein PhnH
RA NZ_CP022686 3,184,651 A→C 11.0% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,213,819 T→G 6.9% R479R (CGT→CGG nnr → bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,231 C→G 9.7% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,516,093 T→G 12.0% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,606,036 G→T 5.7% E250* (GAA→TAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,625,679 A→T 7.0% Y149F (TAC→TTC)  aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,466 T→C 100% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,380 T→G 19.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,520 G→T 7.8% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 16.3% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 14.9% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,868,846 T→G 14.4% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,910,587 A→T 6.0% L155* (TTA→TAA)  yahC ← DUF1097 domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 16.2% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 9.0% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 8.0% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,376 T→G 6.8% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 15.5% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 11.1% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 10.4% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 11.2% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,291 G→T 24.0% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,073,132 C→A 18.0% S404R (AGC→AGA ybaT → amino acid permease
RA NZ_CP022686 4,213,260 T→G 9.6% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,103 T→A 9.4% intergenic (‑3/+35) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,234,399 C→A 12.7% intergenic (+99/‑343) glnS → / → chiP glutamine‑‑tRNA ligase/chitoporin
RA NZ_CP022686 4,270,341 T→G 8.9% V329G (GTC→GGC)  sucA → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,316,394 A→C 8.0% T68P (ACC→CCC)  bioB → biotin synthase BioB
RA NZ_CP022686 4,331,751 T→G 7.3% T352P (ACG→CCG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,902 G→T 7.4% Y301* (TAC→TAA ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,905 A→C 9.3% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,909 G→T 16.7% S299Y (TCC→TAC)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,335,504 T→G 6.9% K58N (AAA→AAC ybhF ← ATP‑binding cassette domain‑containing protein
RA NZ_CP022686 4,413,654 T→G 7.6% K121N (AAA→AAC ltaE ← low‑specificity L‑threonine aldolase
RA NZ_CP022686 4,413,706 T→G 6.7% D104A (GAC→GCC)  ltaE ← low‑specificity L‑threonine aldolase
RA NZ_CP022686 4,440,297 T→A 13.2% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,324 C→A 14.3% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,632,182 T→G 7.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 7.9% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,712,763 A→C 22.9% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 17.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,883 A→T 10.1% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 8.2% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,815,879 T→G 14.7% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,210 T→G 13.7% G112G (GGT→GGG CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,919,017 T→G 9.8% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 5,023,965 C→A 8.5% intergenic (+231/‑247) zntB → / → dbpA zinc transporter ZntB/ATP‑dependent RNA helicase DbpA
RA NZ_CP022686 5,041,191 G→A 5.1% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 11.9% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 7.3% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.4% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,561:1 +C 8.8% coding (31/63 nt) ompA →
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,735 A→T 7.1% K43* (AAA→TAA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 23.6% E52* (GAA→TAA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,806:1 +A 100% intergenic (‑171/+403) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,868 Δ1 bp 100% intergenic (‑233/+341) annotated as hok_sok locus ← / ← putative HTH domain protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 815–2618 2967–2957 340–2153 781 [688] [720] 806 JNG15_RS00010–[mobA] JNG15_RS00010,JNG15_RS00015,[mobA]
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4942–5066 5478–5074 9–537 5157 [4447] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1522881 =NA (NA)2492 (0.650) 166/250 NT 46.4% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3495 = 2875 (0.380)intergenic (‑1699/‑36) putative rom (RNA I inhibition modulator protein)/ompA –/–
* ? NZ_CP022686 = 1524130NA (NA)4066 (1.060) 209/250 NT 58.9% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3497 2832 (0.380)intergenic (‑1701/‑34) putative rom (RNA I inhibition modulator protein)/ompA –/–
* ? NZ_CP022686 3899666 =38 (0.200)195 (1.160) 66/218 NT 88.2% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 19 (0.110)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 389968129 (0.150)178 (1.060) 76/218 NT 88.9% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 19 (0.110)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 4514374 =171 (0.890)5 (0.000)
+AGCT
5/242 NT 5.4% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 11 (0.000)coding (1/399 nt) IL2wt no signal