BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pMut‑Ompa‑D5‑IL2wt‑His6	3,762	G→T	14.1%	E52* (GAA→TAA)	IL2wt no signal →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pMut‑Ompa‑D5‑IL2wt‑His6	3,762	0	G	T	14.1%	13526.4 / inf	5769	E52* (GAA→TAA)	IL2wt no signal
Reads supporting (aligned to +/- strand): ref base G (1697/3196); new base T (283/582); total (1986/3783)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5801 total aligned reads displayed.

ATCGACGAAGAAGACCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTGAAGGG  >  pMut‑Ompa‑D5‑IL2wt‑His6/3623‑3901

      gcagaagaCCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAACAAGCAACAGAATTGa                                                                                                                                       <  4:389556/138‑1 (MQ=255)

      gaagaaGACCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGa                                                                                                                                       <  1:630319/140‑1 (MQ=255)

      gaagaaGACCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGAAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGa                                                                                                                                       <  4:755903/140‑1 (MQ=255)

       aagaagACCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGaa                                                                                                                                      >  3:119728/1‑140 (MQ=255)

        agaagaCCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGaaa                                                                                                                                     <  1:151550/140‑1 (MQ=255)

             aCCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACtt                                                                                                                                <  6:21511/140‑1 (MQ=255)

              cccAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACt                                                                                                                                 <  7:617241/138‑1 (MQ=255)

              cccAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACt                                                                                                                                 <  7:481632/138‑1 (MQ=255)

              cccAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACACAATTGAAACACTTa                                                                                                                               <  6:417730/140‑1 (MQ=255)

              cccAGCAGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCAGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTa                                                                                                                               <  3:437910/140‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGTACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTa                                                                                                                               <  8:156820/138‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGAGCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAATAAGCAACATAATTGAAACACTTa                                                                                                                               <  8:339700/138‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTa                                                                                                                               <  7:74593/138‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTa                                                                                                                               <  7:2316/138‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACa                                                                                                                             <  1:100623/140‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACa                                                                                                                             <  5:635193/140‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACa                                                                                                                             <  5:887357/140‑1 (MQ=255)

                cAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGAGCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACa                                                                                                                             <  5:142850/140‑1 (MQ=255)

                  gCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAgt                                                                                                                           <  1:931847/140‑1 (MQ=255)

                    tGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATTCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAgt                                                                                                                           >  7:290511/1‑138 (MQ=255)

                    tGCAGCTTGAGCATCTTTTATTGGAACTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAACTAACTCGTATGCTTACATTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAgg                                                                                                                           >  8:286315/1‑137 (MQ=255)

                      cAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGttt                                                                                                                       >  6:28732/1‑140 (MQ=255)

                      cAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGttt                                                                                                                       >  1:291901/1‑140 (MQ=255)

                       aGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTAAAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTa                                                                                                                      >  3:244712/1‑140 (MQ=255)

                        gCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTag                                                                                                                     <  4:619316/140‑1 (MQ=255)

                           tGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaag                                                                                                                  <  4:679512/140‑1 (MQ=255)

                           tGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaag                                                                                                                  >  1:493680/1‑140 (MQ=255)

                           tGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaag                                                                                                                  <  5:25745/140‑1 (MQ=255)

                            taGCATCTTTTATTGGACCTGCAGATGCTCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaaga                                                                                                                 <  5:516202/139‑1 (MQ=255)

                            gAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaaga                                                                                                                 <  2:246102/140‑1 (MQ=255)

                             aGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaagaa                                                                                                                >  1:561064/1‑140 (MQ=255)

                              gCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTagaagaag                                                                                                               <  2:670755/140‑1 (MQ=255)

                               cATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGATTAATTTTAAATTTTAAATGCCGAAAAAAGCAACAGAAATGAAACACTTACAATGTTTagaagaa                                                                                                                >  8:810844/1‑138 (MQ=255)

                                   ttttATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTa                                                                                                          >  6:655590/1‑140 (MQ=255)

                                   ttttATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTATATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAATAAGAATTa                                                                                                          >  4:920054/1‑140 (MQ=255)

                                          ggACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGt                                                                                                   >  1:351112/1‑140 (MQ=255)

                                             ccTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTgt                                                                                                >  5:386182/1‑139 (MQ=255)

                                              cTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTg                                                                                                 >  8:617802/1‑138 (MQ=255)

                                                gCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTggagg                                                                                             >  2:587007/1‑140 (MQ=255)

                                                gCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGCAGAATTAAAGCCGTTggagg                                                                                             >  2:7352/1‑140 (MQ=255)

                                                gCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTggagg                                                                                             <  6:353150/140‑1 (MQ=255)

                                                  aGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAATAAGAATTAAAGCCGTTggagg                                                                                             >  7:114197/1‑138 (MQ=255)

                                                      gatCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGa                                                                                                                                       >  1:701661/1‑92 (MQ=255)

                                                       atCTTAAACTGAATCAATAATTACAAAAACACTAATTTAAATAGTATGCTTAATTTTATATTTTATATGCCGAAAAAATCAAAATAATAGAATCACTTACAGTGTTTAGAAGAATAATTATAGCCGTTTGAGGAAGtttt                                                                                      <  6:54158/140‑1 (MQ=255)

                                                         cTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTggagg                                                                                             <  5:760069/131‑1 (MQ=255)

                                                         cTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTaa                                                                                    >  4:326209/1‑140 (MQ=255)

                                                              aCGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGTGGGATGTTTTAAATCtt                                                                               >  4:545457/1‑140 (MQ=255)

                                                               cGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGTAGGAAGTTTTAAATCTTg                                                                              >  3:517461/1‑140 (MQ=255)

                                                                 gcagcaACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGTAGGAAGTTTTAAATCTTGCt                                                                            <  5:821408/140‑1 (MQ=255)

                                                                 gCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAATAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTg                                                                              <  7:556059/138‑1 (MQ=255)

                                                                 gCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCt                                                                            <  1:227305/140‑1 (MQ=255)

                                                                 gCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCt                                                                            <  6:860237/140‑1 (MQ=255)

                                                                 gCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCt                                                                            <  1:860728/140‑1 (MQ=255)

                                                                 gCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCGTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAATAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCt                                                                            <  6:717255/140‑1 (MQ=255)

                                                                  cATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTc                                                                           >  6:478480/1‑140 (MQ=255)

                                                                  cATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAAt                                                                                  >  3:304048/1‑133 (MQ=255)

                                                                  cATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCt                                                                            >  2:92120/1‑139 (MQ=255)

                                                                  cATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTc                                                                           >  4:838865/1‑140 (MQ=255)

                                                                  cATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGAAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCta                                                                           >  5:689811/1‑139 (MQ=255)

                                                                    tCAACAATTACAAAAACCCGATATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAATCCGTAGGATGAAGTTTTAAATCTTGCta                                                                           >  7:407490/1‑137 (MQ=255)

                                                                           ttACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGa                                                                  >  1:417226/1‑140 (MQ=255)

                                                                             aCAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAAt                                                                <  6:142740/140‑1 (MQ=255)

                                                                                aaaaGCCGAAATTAACTCGTATGCGTACATCTAAATGTTATCTGCCGAAAAAAGCAACAGATTTGAAACACTTACAGTGTTTAGCAGAATAATTAAAGACGTTGGAGGAAGTGTTAAATCTTGCTCAATCCAAGAAtt                                                               <  8:559222/138‑1 (MQ=255)

                                                                                        aaaTTAACTCGTATTCTTTCTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACg                                                     <  3:828739/140‑1 (MQ=255)

                                                                                        aaaTTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACg                                                     <  3:649043/140‑1 (MQ=255)

                                                                                             aaCTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAATAAGTATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTcccc                                                <  3:376556/140‑1 (MQ=255)

                                                                                                 cGTATGCTTACTTTTAAAATTTATAAGCCGAAAAAAGCAACAGAATTGAAAAACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTgc                                            <  2:792439/140‑2 (MQ=255)

                                                                                                  gTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTg                                             <  7:178086/138‑1 (MQ=255)

                                                                                                    aTGCTTACTTTTAAATTTTATATGCCGAAAACAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGGTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGAGACCt                                         <  3:185914/140‑1 (MQ=255)

                                                                                                    aTGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGATGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGAc                                           <  7:815442/138‑1 (MQ=255)

                                                                                                    aTGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGAc                                           <  7:880631/138‑1 (MQ=255)

                                                                                                     tGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGAcc                                          <  8:220091/138‑1 (MQ=255)

                                                                                                     tGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAATCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTg                                        <  5:584517/140‑1 (MQ=255)

                                                                                                     tGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGAcc                                          >  7:679873/1‑138 (MQ=255)

                                                                                                     tGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTg                                        <  4:260695/140‑1 (MQ=255)

                                                                                                     tGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTg                                        <  2:196519/140‑1 (MQ=255)

                                                                                                      gCTTACTTTTAAATTTAATATGCAGTAAAAAGCATCAGAATTGCAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTATATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGAGCTGa                                       <  5:451938/140‑1 (MQ=255)

                                                                                                        ttACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTT‑GAGGAAGTTTTAAATCTTGCTCAATCAAAGAATTTCCATTTACGTCCCCGTGACCTGAtct                                    >  3:769543/1‑140 (MQ=255)

                                                                                                            ttttAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTa                                 >  1:773323/1‑140 (MQ=255)

                                                                                                            ttttAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTa                                 >  2:851252/1‑140 (MQ=255)

                                                                                                                aaaTTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAACTCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAAc                               >  8:684109/1‑138 (MQ=255)

                                                                                                                aaaTTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACAt                             <  2:306472/140‑1 (MQ=255)

                                                                                                                      tataTGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTg                                        >  2:392569/1‑123 (MQ=255)

                                                                                                                      tataTGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGt                       <  6:78247/140‑1 (MQ=255)

                                                                                                                        tatGCCGAAAAAAGCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGa                     <  4:115101/140‑1 (MQ=255)

                                                                                                                        tatGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTG‑CCTGATCTCTAACATCAACGTGAt                    <  4:463339/140‑1 (MQ=255)

                                                                                                                        aatGCCGAAAAAAGCAACAGAATTGACACACTTACAGTGTTTAGAAGAAGAATTAACGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGa                     <  2:536095/139‑1 (MQ=255)

                                                                                                                         atGCCTAATAAATCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCCTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCTTGACCTGATCTCTAACATCAACGTGAt                    <  3:898883/140‑1 (MQ=255)

                                                                                                                         atGCCTAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTACATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTg                      <  8:749912/138‑1 (MQ=255)

                                                                                                                         atGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGTATGAAGTTTTAAATCTTGCTCATTCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTg                      <  8:486888/138‑1 (MQ=255)

                                                                                                                         atGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGTAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGAt                    <  2:46999/140‑1 (MQ=255)

                                                                                                                         atGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTg                      <  7:349141/138‑1 (MQ=255)

                                                                                                                         atGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGAt                    <  3:587468/140‑1 (MQ=255)

                                                                                                                          tGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGAtt                   <  3:710113/140‑1 (MQ=255)

                                                                                                                             cGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGCCCTGATCTCTAACATCAACGTGATTGTc                >  5:325289/1‑140 (MQ=255)

                                                                                                                                   acgCAACATAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGGTTTAAATCTTGCTCAATCCAAGAATTTCCTTTTACGGCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTagag          <  2:129747/138‑1 (MQ=255)

                                                                                                                                       aaCAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTGa      <  4:180105/140‑1 (MQ=255)

                                                                                                                                       aaCAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTGa      >  6:6044/1‑140 (MQ=255)

                                                                                                                                        aCAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGGCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTg       <  8:414221/138‑1 (MQ=255)

                                                                                                                                         cAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTGAAg    >  5:192876/1‑140 (MQ=255)

ATCGACGAAGAAGACCCAGCTGCAGCTTGAGCATCTTTTATTGGACCTGCAGATGATCTTAAACGGCATCAACAATTACAAAAACCCGAAATTAACTCGTATGCTTACTTTTAAATTTTATATGCCGAAAAAAGCAACAGAATTGAAACACTTACAGTGTTTAGAAGAAGAATTAAAGCCGTTGGAGGAAGTTTTAAATCTTGCTCAATCCAAGAATTTCCATTTACGTCCCCGTGACCTGATCTCTAACATCAACGTGATTGTCTTAGAGCTGAAGGG  >  pMut‑Ompa‑D5‑IL2wt‑His6/3623‑3901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`