breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 124,727 A→C 5.6% intergenic (‑127/‑72) CIW80_RS00635 ← / → CIW80_RS00640 PTS sugar transporter subunit IIB/GntR family transcriptional regulator
RA NZ_CP022686 133,814 C→A 8.6% A476A (GCG→GCT ydfI ← mannitol dehydrogenase family protein
RA NZ_CP022686 197,824 T→G 5.2% V460G (GTA→GGA)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 210,318 A→C 7.9% intergenic (+19/‑18) nemR → / → nemA DNA‑binding transcriptional regulator NemR/N‑ethylmaleimide reductase
RA NZ_CP022686 236,044 G→T 19.5% R353L (CGT→CTT)  pykF → pyruvate kinase PykF
RA NZ_CP022686 238,323 A→C 24.4% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 9.2% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 269,554 A→C 11.3% G269G (GGT→GGG selO ← protein adenylyltransferase SelO
RA NZ_CP022686 340,485 A→C 7.6% V41G (GTT→GGT)  ydjK ← MFS transporter
RA NZ_CP022686 364,743 T→G 8.6% intergenic (‑178/+27) fadD ← / ← yeaY long‑chain‑fatty‑acid‑‑CoA ligase FadD/Slp family lipoprotein YeaY
RA NZ_CP022686 364,775 C→A 8.7% E193* (GAA→TAA)  yeaY ← Slp family lipoprotein YeaY
RA NZ_CP022686 418,374 T→G 9.1% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 524,842 T→G 9.1% V203G (GTC→GGC)  yedE → selenium metabolism membrane protein YedE/FdhT
RA NZ_CP022686 531,408 A→C 10.3% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
JC NZ_CP022686 532,627 +TGCTGGC 8.8% coding (1351/1659 nt) fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 12.5% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 17.4% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.4% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,833 A→C 10.6% P243P (CCT→CCG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,406 G→T 10.4% intergenic (+166/‑30) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,421 G→T 8.0% intergenic (+181/‑15) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,457 G→T 8.5% E8* (GAA→TAA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 15.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,756 C→G 18.7% D291H (GAC→CAC)  wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 21.2% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 10.1% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 20.1% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 12.8% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,513 C→A 12.3% intergenic (‑102/+125) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 15.3% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,573 C→A 18.1% intergenic (‑146/+77) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,923 C→G 7.9% S595C (TCT→TGT)  yehM → DUF5682 family protein
RA NZ_CP022686 881,760 T→G 16.5% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 13.7% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 14.9% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,114 T→G 10.8% intergenic (+183/‑74) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 955,932 A→C 5.4% F41V (TTC→GTC)  ccmC ← heme exporter protein CcmC
RA NZ_CP022686 957,112 T→G 8.7% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,418 T→G 10.7% T949T (ACA→ACC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 9.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,034,493 T→G 8.2% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,065,997 A→C 11.1% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 12.2% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,120,323 A→T 6.0% T2558T (ACT→ACA CIW80_RS05650 ← phase‑variable autotransporter adhesin UpaE
RA NZ_CP022686 1,145,575 C→A 9.4% E332* (GAA→TAA)  oxc ← oxalyl‑CoA decarboxylase
RA NZ_CP022686 1,172,453 C→A 9.7% D125E (GAC→GAA yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,172,467 C→A 12.3% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,174,272 A→C 10.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 17.5% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,269,551 T→G 8.1% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,280,117 T→G 6.2% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,319,588 T→G 10.2% E332D (GAA→GAC glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,360,724 G→T 7.1% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,729 G→T 7.4% intergenic (‑64/+260) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,742 C→A 6.4% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,277 T→G 6.8% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,371,066 T→A 7.7% V27E (GTA→GAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,081 C→A 14.4% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,086 G→T 21.1% E34* (GAA→TAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,092 T→A 12.9% Y36N (TAC→AAC)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,441,726 A→C 8.8% L612V (TTA→GTA)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,441,772 A→C 7.9% R596R (CGT→CGG hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,460,615 A→C 10.7% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,484,873 C→A 7.0% intergenic (‑208/+57) CIW80_RS07420 ← / ← cysH Hok/Gef family protein/phosphoadenosine phosphosulfate reductase
RA NZ_CP022686 1,504,258 G→T 10.2% S232I (AGT→ATT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 8.4% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 9.4% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 15.6% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,373 G→C 10.3% K270N (AAG→AAC CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,510,233 A→C 5.1% V599G (GTT→GGT)  relA ← GTP pyrophosphokinase
RA NZ_CP022686 1,547,110 G→C 15.0% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,569 A→C 10.6% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,579,273 G→T 6.2% I120I (ATC→ATA CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,579,310 G→C 6.6% S108* (TCA→TGA)  CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,579,312 A→C 8.5% V107V (GTT→GTG CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,597,266 C→G 7.2% M8I (ATG→ATC thyA ← thymidylate synthase
RA NZ_CP022686 1,632,501 T→G 8.5% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,632,508 C→A 8.0% I168I (ATC→ATA arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 24.8% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 12.5% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,640,925 A→C 13.0% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,231 A→T 11.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 11.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 8.6% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,167 A→C 13.0% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,734,237 A→G 100% W156R (TGG→CGG)  papX ← HTH‑type transcriptional regulator PapX
RA NZ_CP022686 1,758,771 T→G 5.8% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,828,110 A→C 6.1% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,832,023 T→G 8.8% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,768 A→C 14.9% M460R (ATG→AGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,925,332 A→C 9.7% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,353 G→T 7.6% G158V (GGT→GTT)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 17.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,954,955 T→G 12.1% Q81P (CAG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,975,137 A→C 6.0% intergenic (+6/‑57) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 2,016,160 T→G 5.7% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 9.8% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 6.7% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,032,739 G→T 6.9% W24L (TGG→TTG)  agaC → PTS galactosamine transporter subunit IIC
RA NZ_CP022686 2,037,521 A→C 10.9% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,047,984 A→C 5.5% R624R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 16.7% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 8.1% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,102 A→C 5.3% A636A (GCT→GCG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,168 A→C 9.2% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,767 A→C 6.1% N379T (AAC→ACC)  argG → argininosuccinate synthase
RA NZ_CP022686 2,061,805 G→C 7.1% G392R (GGC→CGC)  argG → argininosuccinate synthase
RA NZ_CP022686 2,070,788 A→C 6.7% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,110,457 T→G 10.2% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,685 A→C 12.1% intergenic (‑193/+27) rplM ← / ← zapE 50S ribosomal protein L13/cell division protein ZapE
RA NZ_CP022686 2,130,829 A→C 8.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 9.8% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 12.9% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,118 C→G 14.9% A127G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 13.1% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 14.3% F135V (TTC→GTC) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,143 C→G 13.1% F135L (TTC→TTG) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,660 G→T 12.0% D163Y (GAT→TAT)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 5.6% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,184,330 G→T 15.7% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 8.4% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 6.1% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,798 C→G 7.5% intergenic (+40/‑57) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,272,810 T→G 8.8% intergenic (+52/‑45) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,284,668 A→C 7.7% R207R (CGT→CGG malQ ← 4‑alpha‑glucanotransferase
RA NZ_CP022686 2,310,429 T→G 7.0% N248T (AAC→ACC)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 12.3% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 21.2% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 11.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,362,607 T→G 6.3% L36R (CTT→CGT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,386,496 T→G 9.7% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 5.2% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 16.8% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,291 C→A 9.5% E114* (GAA→TAA)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,295 G→T 7.6% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,339 C→A 7.7% D98Y (GAT→TAT)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 13.3% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,516,638 C→A 8.0% T683N (ACC→AAC)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,872 A→C 6.4% K761T (AAA→ACA) ‡ upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,873 A→C 7.9% K761N (AAA→AAC) ‡ upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 12.1% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 15.0% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,368 T→G 5.7% G130G (GGA→GGC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,643,652 T→G 7.9% D433A (GAC→GCC)  dnaA ← chromosomal replication initiator protein DnaA
RA NZ_CP022686 2,701,400 G→T 5.5% R328S (CGC→AGC)  mdtD ← multidrug transporter subunit MdtD
RA NZ_CP022686 2,703,299 A→C 7.3% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,743,563 T→G 13.5% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,769,166 C→A 11.3% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 16.7% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,773,056 T→G 6.9% V51G (GTT→GGT)  rhtC → threonine export protein RhtC
RA NZ_CP022686 2,780,110 T→G 5.9% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,808,127 T→G 8.2% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,821,647 G→C 5.6% intergenic (+103/‑275) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 12.3% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 8.4% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 21.8% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 11.0% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 8.1% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,449 T→G 7.7% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,874,073 A→C 6.2% T62P (ACC→CCC)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,913,769 A→C 8.6% H611P (CAC→CCC)  metL → bifunctional aspartate kinase/homoserine dehydrogenase II
RA NZ_CP022686 2,920,385 C→A 7.5% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,961,318 A→C 7.7% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,597 G→T 12.7% E562* (GAA→TAA)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 7.2% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 11.8% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 9.1% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,071,004 T→G 6.9% G59G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,130,209 A→C 10.8% V240G (GTC→GGC)  alsC ← D‑allose ABC transporter permease
RA NZ_CP022686 3,136,923 A→C 9.0% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 10.0% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,163,655 T→G 6.1% V300G (GTC→GGC)  melA → alpha‑galactosidase
RA NZ_CP022686 3,184,639 A→C 7.5% intergenic (‑86/+30) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 6.7% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,209,153 T→G 6.0% N225T (AAC→ACC)  rsgA ← small ribosomal subunit biogenesis GTPase RsgA
RA NZ_CP022686 3,218,260 T→G 6.0% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,265,096 G→T 17.1% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,062 T→G 9.4% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,342 A→T 7.0% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 8.2% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 9.0% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,616 G→T 6.4% I231I (ATC→ATA sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,387,644 A→C 7.8% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 16.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,444,711 C→A 9.8% L527L (CTG→CTT btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,834 C→A 10.0% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,215 A→T 14.6% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 21.9% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 11.7% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,496,452 A→C 11.0% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,496,461 T→G 12.1% F276V (TTT→GTT)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,504,054 T→G 9.5% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,516,069 T→G 6.7% V211G (GTT→GGT)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,528,214 A→C 7.9% T56P (ACC→CCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,570,234 T→G 6.7% L217V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,604,564 G→T 16.9% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 9.9% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,466 T→C 60.0% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 60.0% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 17.6% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 16.1% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 8.2% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 8.9% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 19.7% L130V (TTA→GTA) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,483 A→T 12.8% L130F (TTA→TTT) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 13.8% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,544 C→A 8.3% P151T (CCT→ACT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,801,492 T→G 6.6% pseudogene (1768/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,824,332 T→G 8.5% A217A (GCT→GCG CIW80_RS19155 → TolC family protein
RA NZ_CP022686 3,868,846 T→G 10.8% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,534 T→G 9.3% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 13.7% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 9.3% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 12.0% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,937,229 A→C 8.0% V70G (GTG→GGG)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 12.8% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.0% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 6.0% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,977,611 A→C 7.7% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 7.2% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 3,984,841 T→G 8.2% R67R (CGT→CGG queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA
RA NZ_CP022686 4,014,238 C→G 10.9% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 10.5% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 8.7% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 19.5% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,276 G→T 14.8% R101L (CGT→CTT)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,942 C→G 7.5% R131G (CGT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 18.1% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,987 G→C 8.0% D146H (GAC→CAC)  adk → adenylate kinase
RA NZ_CP022686 4,151,277 T→G 6.5% T73P (ACC→CCC)  fepB ← Fe2+‑enterobactin ABC transporter substrate‑binding protein
RA NZ_CP022686 4,213,260 T→G 7.0% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,435 G→C 8.1% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,457 G→T 8.6% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,290,599 T→G 6.9% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,368,341 T→G 13.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 11.9% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 10.6% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,324 C→A 11.4% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→T 20.1% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,562,600 T→G 12.4% Q123P (CAG→CCG)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,571,417 T→G 7.9% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,439 T→G 7.4% H59P (CAC→CCC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,454 A→C 7.8% V54G (GTC→GGC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 13.3% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,584 C→G 8.3% R54P (CGC→CCC)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,605,593 T→G 9.1% Q51P (CAG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 10.8% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 8.9% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,041 A→C 21.6% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,108 C→A 6.3% intergenic (+106/‑15) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,681,602 G→T 5.6% G8C (GGT→TGT)  CIW80_RS23465 → DUF932 domain‑containing protein
RA NZ_CP022686 4,759,434 C→A 5.7% L199F (TTG→TTT ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,764,904 G→T 7.3% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 14.1% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,809,441 T→G 5.1% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,961 T→G 9.8% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,963 C→G 7.6% L62V (CTG→GTG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 10.2% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,886 T→G 9.3% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,839,668 T→G 12.7% V150V (GTA→GTC minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 12.1% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,845,144 A→C 6.4% L101L (CTA→CTC umuD → translesion error‑prone DNA polymerase V autoproteolytic subunit
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 16.4% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,108 A→T 9.2% intergenic (‑5/+42) CIW80_RS24835 ← / ← CIW80_RS24840 RtT sRNA/tRNA‑Tyr
RA NZ_CP022686 4,919,017 T→G 7.6% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,538 C→A 20.4% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 5,042,289 T→C 8.0% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 6.7% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 7.1% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 7.4% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,137 G→T 12.9% V1256V (GTG→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 9.5% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,215 T→G 12.5% N1282K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,484 T→G 13.0% intergenic (‑1688/‑47) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 33.0% E52* (GAA→TAA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,792 G→T 7.3% E62* (GAA→TAA)  IL2wt no signal →
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 8.1% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 663–2618 3067–2957 340–2405 8163 [7533] [7770] 7958 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4745–4809 5478–5214 406–734 5268 [5245] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 1415492 =171 (0.710)11 (0.050) 9/246 NT 6.1% coding (468/531 nt) emrR multidrug efflux transporter EmrAB transcriptional repressor EmrR
?NZ_CP022686 = 1761695 NA (NA)intergenic (‑70/‑72) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 = 1415498170 (0.700)19 (0.080) 15/246 NT 10.2% coding (474/531 nt) emrR multidrug efflux transporter EmrAB transcriptional repressor EmrR
?NZ_CP022686 = 1524128 NA (NA)intergenic (+6/+234) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2700948NA (NA)43 (0.010)
+23 bp
23/204 NT 89.8% intergenic (+11/+6) CIW80_RS13525/mdtD IS3‑like element ISEc52 family transposase/multidrug transporter subunit MdtD
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 6 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3899666 =255 (1.050)78 (0.370) 41/218 NT 27.5% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 190 (0.900)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681245 (1.010)80 (0.380) 43/218 NT 28.4% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 190 (0.900)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 4514374 =234 (0.970)10 (0.000)
+AGCT
8/242 NT 7.9% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 6 (0.000)coding (1/399 nt) IL2wt no signal
* ? pMut‑Ompa‑D5‑IL2wt‑His6 = 17610 (0.000)3 (0.000) 3/246 NT 100% coding (36/195 nt) putative rom (RNA I inhibition modulator protein)
?pMut‑Ompa‑D5‑IL2wt‑His6 1765 = NA (NA)coding (32/195 nt) putative rom (RNA I inhibition modulator protein)