breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 197,854 T→G 9.1% V470G (GTT→GGT)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 201,794 T→G 8.7% K93N (AAA→AAC mliC ← C‑type lysozyme inhibitor
RA NZ_CP022686 329,133 C→T 13.9% intergenic (‑81/‑80) ydjA ← / → sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
RA NZ_CP022686 349,123 A→C 7.8% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 364,743 T→G 9.3% intergenic (‑178/+27) fadD ← / ← yeaY long‑chain‑fatty‑acid‑‑CoA ligase FadD/Slp family lipoprotein YeaY
RA NZ_CP022686 402,874 A→C 7.5% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 418,374 T→G 10.8% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,940 A→T 16.2% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 10.5% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 597,830 A→C 15.4% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 17.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 12.7% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 9.7% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 692,994 G→T 9.6% D112Y (GAT→TAT)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,001 A→T 8.5% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,757 T→G 16.7% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 23.1% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 22.3% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 17.9% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 845,497 A→C 5.6% V267G (GTC→GGC)  apbC ← iron‑sulfur cluster carrier protein ApbC
RA NZ_CP022686 881,739 A→C 18.3% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 937,864 G→A 100% G29R (GGA→AGA)  CIW80_RS04770 → integrase domain‑containing protein
RA NZ_CP022686 948,029 C→A 14.7% intergenic (+98/‑159) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 16.4% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 15.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 8.5% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,082 T→A 12.9% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 18.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,909 A→C 10.2% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,172,467 C→A 16.8% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,174,272 A→C 13.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,213,479 T→G 15.6% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,918 C→A 11.1% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,239,833 G→T 13.9% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,272,651 T→G 8.2% N91T (AAC→ACC)  CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,360,709 T→G 8.4% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 11.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 15.7% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,372,377 T→G 14.9% intergenic (+42/‑62) raiA → / → pheL ribosome‑associated translation inhibitor RaiA/pheA operon leader peptide PheL
RA NZ_CP022686 1,383,405 T→G 6.8% F240V (TTT→GTT)  ypjD → inner membrane protein YpjD
RA NZ_CP022686 1,424,352 A→T 8.4% intergenic (‑48/+15) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,441,800 T→G 9.1% N587T (AAC→ACC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,447,199 A→C 10.8% V35G (GTA→GGA)  hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,479,619 T→A 9.2% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,481,375 A→C 8.2% V272G (GTG→GGG)  cysN ← sulfate adenylyltransferase subunit CysN
RA NZ_CP022686 1,504,361 G→T 20.3% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,373 G→C 19.8% K270N (AAG→AAC CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,524,167 T→G 5.9% intergenic (+45/+195) CIW80_RS07590 → / ← syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
RA NZ_CP022686 1,558,395 A→C 12.3% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,543 A→C 8.6% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,569 A→C 12.0% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 10.5% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 30.0% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 9.9% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 12.2% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 11.9% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,167 A→C 10.2% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,767,277 T→G 6.4% intergenic (‑155/+136) CIW80_RS08760 ← / ← CIW80_RS08770 hypothetical protein/hypothetical protein
RA NZ_CP022686 1,828,110 A→C 6.4% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,833,749 G→T 16.4% V466V (GTC→GTA gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,762 A→C 16.4% L462R (CTG→CGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,925,332 A→C 9.3% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 17.3% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,225 T→G 9.2% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 13.5% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 14.5% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,554 T→G 11.7% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,101,798 G→T 9.4% E250* (GAA→TAA)  gltD → glutamate synthase subunit GltD
RA NZ_CP022686 2,148,115 T→G 13.6% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 9.4% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 15.0% F135V (TTC→GTC) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,143 C→G 13.9% F135L (TTC→TTG) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 10.5% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 7.0% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,207,122 A→C 9.0% intergenic (‑146/+23) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,209,103 T→A 5.6% I249F (ATT→TTT)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,831 G→T 7.5% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,275,771 A→C 6.3% D47A (GAC→GCC)  feoA → ferrous iron transporter A
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 9.0% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,380,936 A→C 7.7% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,394,287 T→G 14.2% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,291 C→G 14.9% E114Q (GAA→CAA)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,885 A→C 14.3% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 13.6% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 11.0% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 23.5% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 9.9% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,577 A→G 8.4% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 13.5% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,572,705 A→C 11.7% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 12.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 9.5% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,736,578 C→A 9.9% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.9% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,582 A→C 13.6% noncoding (84/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,109 A→C 8.2% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 6.9% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,144 A→C 9.7% T46P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,769,154 G→C 9.3% S61C (TCC→TGC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,179 G→C 14.8% L53V (CTG→GTG)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,868,740 T→G 25.8% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,856 T→G 9.1% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 6.3% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,418 C→A 7.2% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,239 T→G 6.2% T209P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,961,318 A→C 9.4% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,612 A→C 10.3% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,994,693 T→G 10.4% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,017,622 A→C 16.6% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 13.8% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,058,442 A→T 5.7% Y316N (TAC→AAC)  CIW80_RS15235 ← FAD‑binding protein
RA NZ_CP022686 3,080,433 T→G 6.3% V149G (GTC→GGC)  CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,114,653 A→C 5.7% V36G (GTC→GGC)  CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,136,940 T→G 11.3% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,252 T→G 10.0% F20V (TTT→GTT)  rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,184,651 A→C 7.7% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,317,667 A→T 15.6% intergenic (+85/+34) lptG → / ← yjgR LPS export ABC transporter permease LptG/DUF853 domain‑containing protein
RA NZ_CP022686 3,348,357 T→G 7.1% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,605 T→G 17.3% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 11.4% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,444,711 C→A 8.1% L527L (CTG→CTT btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,200 C→A 12.6% intergenic (‑9/+26) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 12.3% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 16.9% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 17.4% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,468,365 C→G 5.6% G302G (GGC→GGG yjjU → patatin family protein
RA NZ_CP022686 3,493,370 T→G 7.2% K195N (AAA→AAC arcA ← two‑component system response regulator ArcA
RA NZ_CP022686 3,638,635 T→G 5.1% T10P (ACG→CCG)  speE ← polyamine aminopropyltransferase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 9.8% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,714,337 A→C 8.8% D273A (GAC→GCC)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,734,367 T→G 27.6% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 17.3% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,748,050 T→G 5.3% K22N (AAA→AAC CIW80_RS18735 ← hypothetical protein
RA NZ_CP022686 3,770,509 A→T 12.2% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 12.4% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,868,846 T→G 11.3% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,534 T→G 8.1% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 20.0% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,296 A→C 7.3% E158D (GAA→GAC prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 16.4% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,940,707 A→C 8.9% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 11.4% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 7.3% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,949,972 T→G 8.2% N140T (AAC→ACC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 13.5% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,242 A→T 12.5% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,133,850 T→G 14.9% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,270,799 A→C 9.6% T482P (ACG→CCG)  sucA → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,290,635 T→G 6.4% T109P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,368,341 T→G 14.6% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 13.5% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,269 A→C 5.2% F246V (TTT→GTT)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,334 A→C 21.1% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,570,086 A→C 6.5% L68V (TTA→GTA)  pgaD ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine biosynthesis protein PgaD
RA NZ_CP022686 4,587,091 T→G 13.4% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,579 T→G 15.4% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,620,262 A→C 8.0% intergenic (+6/+117) CIW80_RS23110 → / ← CIW80_RS26565 Exc2 family lipoprotein/hypothetical protein
RA NZ_CP022686 4,642,951 A→C 9.8% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,047 A→C 24.6% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,783,334 A→C 15.9% V32G (GTC→GGC)  mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,809,441 T→G 8.6% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,916,128 A→C 6.3% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,951,512 G→T 17.3% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,951,531 C→A 10.4% S42* (TCA→TAA)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,894 G→C 9.3% L102V (CTT→GTT)  sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 4,980,895 A→C 12.8% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,043,558 T→C 6.9% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.4% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,178 T→G 13.1% I1270S (ATC→AGC)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,637 C→G 75.5% T10S (ACC→AGC) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,638 C→G 75.7% T10T (ACC→ACG) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,639 C→T 76.0% Q11* (CAG→TAG)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,642 C→G 75.6% L12V (CTG→GTG) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,643 T→G 76.0% L12R (CTG→CGG) ‡ IL2wt no signal →
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 7.9% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3084–2957 340–2896 14737 [13531] [13707] 14789 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4746–4861 5478–5074 214–733 6580 [6364] [0] NA [putative HTH domain protein] [putative HTH domain protein]