breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 12.1% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 235,846 A→C 6.8% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 340,516 A→C 9.1% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,100 A→C 14.3% T605P (ACG→CCG)  yeaG → protein kinase YeaG
RA NZ_CP022686 350,056 A→C 14.0% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 13.4% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,236 C→A 17.2% D32Y (GAT→TAT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,269 T→G 9.7% T21P (ACT→CCT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 436,591 A→C 7.5% intergenic (‑19/+38) CIW80_RS02305 ← / ← CIW80_RS02310 phage minor tail protein L/phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 13.8% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 556,942 G→T 18.1% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,948 A→T 12.5% intergenic (+132/‑716) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,957 C→A 12.8% intergenic (+141/‑707) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.0% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.7% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,844 G→C 8.2% Q240E (CAA→GAA)  clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,021 A→C 9.6% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 20.1% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 14.9% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 14.0% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,573 C→A 24.2% intergenic (‑146/+77) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 11.9% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 859,954 A→C 10.3% R605R (CGA→CGC yehM → DUF5682 family protein
RA NZ_CP022686 881,742 A→C 17.5% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 937,864 G→A 100% G29R (GGA→AGA)  CIW80_RS04770 → integrase domain‑containing protein
RA NZ_CP022686 948,023 G→T 10.6% intergenic (+92/‑165) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,098 G→T 13.4% intergenic (+167/‑90) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 10.4% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 13.8% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,022,752 T→G 7.5% T44P (ACG→CCG)  yfaZ ← YfaZ family outer membrane protein
RA NZ_CP022686 1,034,493 T→G 9.5% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,151,773 T→G 5.7% N379T (AAC→ACC)  alaC ← alanine transaminase
RA NZ_CP022686 1,174,272 A→C 13.9% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 24.1% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,237,918 C→A 9.6% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,280,117 T→G 8.8% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,742 C→A 6.6% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,093 A→C 12.2% Y36S (TAC→TCC)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,396,464 T→G 8.9% intergenic (+70/‑167) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,424,362 A→T 9.6% intergenic (‑58/+5) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,447,198 T→A 9.6% V35V (GTA→GTT hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,459,108 T→G 7.7% V171G (GTC→GGC)  flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,619 T→A 15.7% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,257 A→T 11.6% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,277 C→A 8.3% I238I (ATC→ATA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,289 T→G 11.4% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,370 A→C 6.7% D456A (GAC→GCC)  vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,619,248 G→T 7.0% intergenic (+37/+280) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,633,846 T→G 30.4% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 10.0% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.7% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 8.5% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.6% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,697,914 T→G 10.6% intergenic (+23/+196) CIW80_RS08360 → / ← tkt prohibitin family protein/transketolase
RA NZ_CP022686 1,706,032 G→T 9.6% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,167 A→C 11.7% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,925,332 A→C 9.3% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 25.7% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,990,409 A→C 6.1% F423V (TTT→GTT)  uxaA ← altronate dehydratase
RA NZ_CP022686 2,016,160 T→G 7.3% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 8.9% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 8.5% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 7.2% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,047,987 A→C 7.4% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 7.3% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 10.2% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,193 A→C 6.5% V606G (GTT→GGT)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,805 G→C 6.6% G392R (GGC→CGC)  argG → argininosuccinate synthase
RA NZ_CP022686 2,110,465 T→G 10.6% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 12.0% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,115 T→G 13.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 11.9% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.3% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 7.8% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,207,100 C→A 13.1% intergenic (‑124/+45) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,215 A→C 6.9% F38V (TTC→GTC)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,226,139 A→C 6.7% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,098 T→G 5.9% F83V (TTT→GTT)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,357,324 A→C 18.6% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,393,013 T→G 5.6% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,291 C→G 19.9% E114Q (GAA→CAA)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,516,577 A→G 8.9% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,386 C→A 9.7% A98S (GCC→TCC)  waaG ← glycosyltransferase family 4 protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,736,586 A→C 11.8% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,793,316 C→A 8.1% D396Y (GAT→TAT)  fdrA ← acyl‑CoA synthetase FdrA
RA NZ_CP022686 2,808,127 T→G 10.4% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,810,040 A→C 7.9% N242T (AAC→ACC)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,834,886 A→C 8.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 23.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,856 T→G 11.2% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 9.0% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,919,048 A→C 5.9% L349V (TTA→GTA)  CIW80_RS14605 ← hypothetical protein
RA NZ_CP022686 2,920,408 C→A 6.7% Q62K (CAA→AAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 3,020,229 T→G 12.9% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,986 T→G 10.6% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,080,495 T→G 7.2% F170V (TTC→GTC)  CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,136,940 T→G 9.2% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.7% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,252,190 T→G 15.1% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,281,004 T→G 11.2% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,059 T→G 14.2% L93R (CTG→CGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,360 T→G 8.1% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,345 G→T 5.9% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 11.1% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,359 A→T 5.4% intergenic (‑175/‑25) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,082 G→T 9.4% intergenic (+781/‑10) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,376,248 A→C 5.0% K152N (AAA→AAC mtnA → S‑methyl‑5‑thioribose‑1‑phosphate isomerase
RA NZ_CP022686 3,387,116 A→C 11.9% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.3% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,224 T→G 14.6% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 12.0% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 10.6% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,510,388 A→C 9.0% T284P (ACC→CCC)  dnaJ → molecular chaperone DnaJ
RA NZ_CP022686 3,547,355 T→G 7.0% V99G (GTT→GGT)  folA → type 3 dihydrofolate reductase
RA NZ_CP022686 3,547,368 C→A 5.9% F103L (TTC→TTA folA → type 3 dihydrofolate reductase
RA NZ_CP022686 3,604,564 G→T 15.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 12.3% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,528 C→A 5.6% E769* (GAA→TAA)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,655,541 G→C 5.7% V764V (GTC→GTG htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 17.8% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 16.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 19.3% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 15.4% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,256 G→T 13.7% E1583* (GAA→TAA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,852,459 T→G 9.6% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,857 T→G 7.9% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,928,345 T→G 9.4% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.1% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 12.1% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 7.2% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,631 A→C 9.9% H80P (CAC→CCC)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 14.2% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,381 T→G 9.3% L96R (CTT→CGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,238 C→G 10.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,951 C→A 16.9% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 19.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,074,902 G→T 14.4% S23* (TCG→TAG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,079,332 A→C 8.7% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,133,850 T→G 9.8% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,280,095 T→G 7.5% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,331,940 A→C 17.1% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 8.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 15.3% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 8.1% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,387 A→C 8.1% C32G (TGT→GGT)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,439,653 A→C 9.8% K570N (AAA→AAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,314 T→G 12.2% Y791D (TAT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,587,091 T→G 9.7% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 8.1% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 8.7% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,661,459 T→G 7.6% pseudogene (559/655 nt) CIW80_RS26570 → hypothetical protein
RA NZ_CP022686 4,678,033 T→G 40.1% intergenic (+31/‑90) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 25.4% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 12.8% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 16.1% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,839,687 T→G 8.9% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 15.2% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,910,213 A→C 9.0% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,916,128 A→C 5.7% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,951,512 G→T 9.8% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,164 A→C 11.0% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,023,943 C→G 6.7% intergenic (+209/‑269) zntB → / → dbpA zinc transporter ZntB/ATP‑dependent RNA helicase DbpA
RA NZ_CP022686 5,042,250 A→T 8.1% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,294 G→C 5.5% S975S (TCG→TCC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 5.2% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 7.1% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 12.9% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 380–2618 3057–2957 340–2678 5557 [5123] [5363] 5389 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4751–4810 5415–5214 405–665 3729 [3709] [3463] 3869 [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3743172 =231 (1.170)14 (0.070) 12/248 NT 5.5% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 255 (1.310)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase
* ? NZ_CP022686 4514374 =215 (1.090)7 (0.000)
+AGCT
5/242 NT 6.1% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 7 (0.000)coding (1/399 nt) IL2wt no signal