breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 360,227 T→G 20.1% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,948 A→T 16.7% intergenic (+132/‑716) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,302 T→G 19.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 937,864 G→A 100% G29R (GGA→AGA)  CIW80_RS04770 → integrase domain‑containing protein
RA NZ_CP022686 948,090 C→A 12.2% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
JC NZ_CP022686 1,061,329 Δ6 bp 5.3% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,065,997 A→C 11.6% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,145,570 C→G 13.3% L333F (TTG→TTC oxc ← oxalyl‑CoA decarboxylase
RA NZ_CP022686 1,174,272 A→C 12.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,279 A→T 7.1% intergenic (+8/‑36) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 15.7% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 14.8% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,239,833 G→T 18.3% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,504,361 G→T 23.1% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,632,501 T→G 10.8% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 30.1% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 18.6% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 15.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 14.6% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 13.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,047 G→T 12.6% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,745,268 A→C 10.8% L183R (CTG→CGG)  CIW80_RS08660 ← MFS transporter
RA NZ_CP022686 1,805,056 A→C 12.0% pseudogene (337/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,828,113 A→C 11.0% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,965,173 A→C 11.3% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,170 A→C 11.0% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 12.6% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 17.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.4% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,130,829 A→C 7.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,132 C→G 12.9% L132V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.9% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 8.4% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 10.9% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,250,454 A→C 7.0% S40A (TCG→GCG)  dam ← adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 2,272,831 G→T 9.8% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 20.1% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,394,287 T→G 18.8% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,447,824 G→T 6.3% L26M (CTG→ATG)  CIW80_RS12290 ← type I toxin‑antitoxin system toxin Ldr family protein
RA NZ_CP022686 2,460,581 A→C 24.2% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,546,394 A→C 13.1% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 17.9% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,558,864 T→G 9.4% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,616,198 G→T 59.4% G260C (GGT→TGT)  CIW80_RS13115 → hypothetical protein
RA NZ_CP022686 2,736,585 T→A 7.9% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,834,886 A→C 9.0% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 21.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,950,876 C→G 60.5% H26Q (CAC→CAG oxyR → DNA‑binding transcriptional regulator OxyR
RA NZ_CP022686 3,020,229 T→G 15.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 23.7% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 17.4% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,834 C→A 11.8% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 30.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,520,304 A→C 5.9% N355T (AAC→ACC)  ileS → isoleucine‑‑tRNA ligase
RA NZ_CP022686 3,652,079 T→G 13.0% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 13.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 18.3% L130V (TTA→GTA) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,483 A→T 19.2% L130F (TTA→TTT) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,928,369 T→G 13.5% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 18.3% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 11.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 4,118,534 A→T 8.5% Y256N (TAC→AAC)  nfrA ← bacteriophage adsorption protein NfrA
RA NZ_CP022686 4,440,334 A→G 18.9% Q797Q (CAA→CAG ftsK → DNA translocase FtsK
RA NZ_CP022686 4,632,182 T→G 10.2% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,938 A→C 9.9% T728P (ACG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,951,531 C→A 11.6% S42* (TCA→TAA)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,041,191 G→A 8.2% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,041,356 T→A 9.4% G329G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,300 C→T 7.2% G977G (GGC→GGT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,637 C→G 94.3% T10S (ACC→AGC) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,638 C→G 94.6% T10T (ACC→ACG) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,639 C→T 94.5% Q11* (CAG→TAG)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,642 C→G 94.4% L12V (CTG→GTG) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,643 T→G 94.5% L12R (CTG→CGG) ‡ IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,841 G→T 14.2% intergenic (‑206/+368) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,868 Δ1 bp 100% intergenic (‑233/+341) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,913 A→T 8.2% intergenic (‑278/+296) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,915 G→T 9.6% intergenic (‑280/+294) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,916 G→T 10.1% intergenic (‑281/+293) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,922 G→T 13.7% intergenic (‑287/+287) annotated as hok_sok locus ← / ← putative HTH domain protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3086–2957 340–2898 11253 [10377] [11078] 11569 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA