breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 75,475 C→G 8.2% D97H (GAC→CAC)  dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA NZ_CP022686 177,506 T→G 7.2% Q140H (CAA→CAC uidB ← glucuronide transporter
RA NZ_CP022686 298,027 A→C 12.5% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,106 A→C 13.7% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 17.4% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,942 G→T 15.4% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 11.7% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 14.6% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,713 A→C 10.9% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,848 A→C 11.8% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,391 C→A 11.2% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,457 G→T 14.5% E8* (GAA→TAA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 16.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,014 A→T 11.3% V35V (GTT→GTA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 19.1% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 15.5% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 878,883 T→G 8.2% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 1,000,401 A→C 11.6% V955G (GTG→GGG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 7.0% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 13.8% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,047,906 G→T 7.5% intergenic (‑136/+28) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,065,978 A→C 9.9% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,174,272 A→C 19.9% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,310 G→T 9.6% intergenic (+39/‑5) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 22.2% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 20.6% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,935 A→C 8.6% G297G (GGT→GGG tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,249,393 C→A 9.0% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,269,551 T→G 7.3% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,276,970 A→C 8.7% G414G (GGT→GGG der ← ribosome biogenesis GTPase Der
RA NZ_CP022686 1,280,117 T→G 7.5% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,353,233 A→C 10.5% V72G (GTT→GGT)  yfiF ← tRNA/rRNA methyltransferase
RA NZ_CP022686 1,360,724 G→T 13.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,504,310 G→T 12.3% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,339 C→A 18.8% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 21.7% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,353 G→T 34.4% M31I (ATG→ATT fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,365 A→C 27.8% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,619,248 G→T 7.5% intergenic (+37/+280) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,501 T→G 8.9% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,674,224 T→G 13.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 14.1% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,730,016 T→G 7.9% R41R (CGT→CGG CIW80_RS08540 → integrase arm‑type DNA‑binding domain‑containing protein
RA NZ_CP022686 1,744,265 A→C 6.4% N329T (AAC→ACC)  CIW80_RS08650 → IS3 family transposase
RA NZ_CP022686 1,785,695 T→G 8.2% pseudogene (16/186 nt) CIW80_RS08875 ← zinc‑ribbon domain‑containing protein
RA NZ_CP022686 1,833,745 T→G 19.7% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,889,761 A→C 5.9% V459G (GTT→GGT)  ygiQ ← YgiQ family radical SAM protein
RA NZ_CP022686 1,925,332 A→C 11.6% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 15.3% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,144 C→G 8.9% R96P (CGC→CCC)  nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,049,097 A→T 14.9% R253R (CGT→CGA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,588 C→A 6.6% intergenic (+58/‑175) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 10.2% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 8.5% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,109 T→G 9.8% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 10.4% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 10.4% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 14.4% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,229 G→T 7.8% intergenic (‑98/+67) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,394,287 T→G 23.5% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,885 A→C 8.2% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 12.3% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,526 A→C 9.5% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 12.8% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 24.5% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 10.4% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 11.0% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,423 A→C 10.1% V85V (GTT→GTG waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,574,429 T→G 12.7% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 6.2% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,632,838 A→C 9.0% A53A (GCT→GCG dgoT ← MFS transporter
RA NZ_CP022686 2,677,646 T→G 8.6% K82N (AAA→AAC atpD ← F0F1 ATP synthase subunit beta
RA NZ_CP022686 2,717,385 T→G 6.7% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,718,627 T→G 6.4% F510V (TTC→GTC)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,585 T→A 10.0% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,754,975 C→A 5.8% E5* (GAA→TAA)  cyaY ← iron donor protein CyaY
RA NZ_CP022686 2,758,109 A→C 7.7% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 10.6% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 17.5% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,780,110 T→G 7.9% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,793,267 T→G 6.2% D412A (GAC→GCC)  fdrA ← acyl‑CoA synthetase FdrA
RA NZ_CP022686 2,801,817 T→G 10.0% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,834,886 A→C 10.6% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 21.6% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 13.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.5% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,961,318 A→C 11.9% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,612 A→C 6.9% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,625 A→C 6.4% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 3,020,188 C→A 13.0% F119L (TTC→TTA yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,023,689 T→G 5.1% V55V (GTA→GTC sorE ← L‑sorbose 1‑phosphate reductase
RA NZ_CP022686 3,136,923 A→C 11.1% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,163,972 G→T 7.9% E406* (GAA→TAA)  melA → alpha‑galactosidase
RA NZ_CP022686 3,177,069 T→G 5.2% T249P (ACC→CCC)  cadA ← lysine decarboxylase CadA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 6.8% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,297,333 T→G 11.7% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,297,360 T→G 7.3% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,324,171 T→G 6.4% pseudogene (400/467 nt) CIW80_RS16540 ← DUF1643 domain‑containing protein
RA NZ_CP022686 3,348,342 A→T 9.8% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,614 G→T 9.7% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,215 A→T 10.0% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 13.6% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 20.2% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,574,991 A→C 6.1% F182V (TTC→GTC)  sgrR ← DNA‑binding transcriptional regulator SgrR
RA NZ_CP022686 3,604,564 G→T 13.8% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,628,850 A→C 8.6% K313N (AAA→AAC aceF → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
RA NZ_CP022686 3,652,079 T→G 11.3% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 20.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 13.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,776,894 G→T 6.1% L132F (TTG→TTT CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 12.4% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,092 C→A 8.7% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,852,459 T→G 7.6% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 12.7% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,864 A→C 11.1% S377A (TCG→GCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,455 T→A 10.6% I1403I (ATT→ATA fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,925,304 A→C 11.4% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,977,656 A→C 10.6% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,381 T→G 7.5% L96R (CTT→CGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,242 A→T 8.8% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,019,028 A→C 7.9% D259A (GAC→GCC)  lon → endopeptidase La
RA NZ_CP022686 4,032,292 A→C 17.3% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 16.9% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,987 G→C 10.6% D146H (GAC→CAC)  adk → adenylate kinase
RA NZ_CP022686 4,133,850 T→G 14.9% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,214,457 G→T 11.9% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,290,599 T→G 8.3% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,905 A→C 10.8% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,372 T→G 11.3% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 11.3% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 13.8% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 10.7% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,285 A→C 9.7% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,562,574 C→G 9.0% A132P (GCC→CCC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,632,182 T→G 8.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,712,763 A→C 18.4% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 17.2% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 8.7% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 12.4% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 11.1% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,441 T→G 9.0% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,990 T→G 9.4% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,221 T→G 16.0% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,873 T→G 7.6% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,536 G→C 10.6% A44P (GCC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,164 A→C 11.1% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→G 12.4% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 6.8% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 7.9% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.1% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 94.1% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 187–2618 3085–2957 340–2899 9744 [9626] [9556] 9695 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1263–1317 3984–1582 266–2722 3802 [3537] [3690] 3762 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 2705754 =NA (NA)4 (0.020) 4/234 NT NA noncoding (283/2903 nt) CIW80_RS13550 23S ribosomal RNA
?NZ_CP022686 = 2705755 NA (NA)noncoding (284/2903 nt) CIW80_RS13550 23S ribosomal RNA
* ? pMUT1‑hok‑empty 456 =2873 (0.450)4229 (0.670) 186/250 NT 67.3% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1229 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4914213 (0.660)3175 (0.630) 140/200 NT 58.0% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1228 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–