BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,286,882	C→A	6.3%	Q63K (CAA→AAA)	cybC →	cytochrome b562

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,286,882	0	C	A	6.3%	611.3 / 3.6	234	Q63K (CAA→AAA)	cybC	cytochrome b562
Reads supporting (aligned to +/- strand): ref base C (133/81); new base A (3/17); total (136/98)
Fisher's exact test for biased strand distribution p-value = 5.83e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CAGTTCGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAG  >  NZ_CP022686/3286743‑3287020

 tgTTCGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCa                                                                                                                                           <  7:355787/139‑1 (MQ=255)

   ttCGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATACCGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCaaa                                                                                                                                         >  5:886934/1‑140 (MQ=255)

   ttCGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAAATAAAATGCGCGCTGCGGCGCTGGATGCGCaaa                                                                                                                                         >  1:970738/1‑140 (MQ=255)

   ttCGGCGTCGTTTGCCGCGGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGCAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCaaa                                                                                                                                         >  7:710709/1‑140 (MQ=255)

     cGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCaaaaa                                                                                                                                       >  8:889079/1‑140 (MQ=255)

      ggCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAg                                                                                                                                      >  7:97530/1‑140 (MQ=255)

        cgtcgtTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTAGAAAAAGCCGATAAAGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCg                                                                                                                                    >  3:888240/1‑140 (MQ=255)

           cgtTTGCCGCTGATCTGNAANACANTATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGAcg                                                                                                                                 >  3:115912/1‑140 (MQ=255)

           cgtTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCTTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGAcg                                                                                                                                 >  6:259484/1‑140 (MQ=255)

             tttGCCGCTGATCTGGAAGCCAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACTCGTTAACTAAAATTCGCGCTTCGGAGCTGGATGCGCAAAAAGCGAcgcc                                                                                                                               >  6:514260/1‑140 (MQ=255)

             tttGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGAcgcc                                                                                                                               >  6:1181025/1‑140 (MQ=255)

             tttGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGAcgcc                                                                                                                               >  6:1124897/1‑140 (MQ=255)

                 ccGCTTATCTGGAAGACAATATGGAAACCCTCAACTACAATTAAAATGTGGGCGAAAACGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCGGCGGCGCTGGATGCGCAAAAAGCGACGccgccg                                                                                                                           <  5:787614/140‑1 (MQ=255)

                    cTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAg                                                                                                                        >  1:1337924/1‑140 (MQ=255)

                       aTCTGGAAGACAATATGGAAACCCTCAACTCAAATTAAAAAGGTGTCTAAAACGCCGATAACGCGGTGCAAGTCAAAGACGCGTAAAATAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCtt                                                                                                                     <  5:1298278/140‑1 (MQ=255)

                         gtgtaaGACAATATGGAAACCCAAAACTACAATTTAAAAGTGGTCGAAAAAGCCCATAACGCGGTGCAAGTCAATGACGCGTTAACTAAAATCCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGa                                                                                                                   <  7:908876/136‑1 (MQ=255)

                            gAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATACCGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCGGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAgg                                                                                                                <  4:504074/140‑1 (MQ=255)

                            gAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAgg                                                                                                                >  1:281548/1‑140 (MQ=255)

                              acaaaaTATGGAAACCCTCAACTACAATTTAAATGTGGGCGAAAAAGCCGATACCGCGGGGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCGGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  1:183798/136‑1 (MQ=255)

                              acaaaaTATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAACCCGATAACGCGGTGCAAGGCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  1:625996/136‑1 (MQ=255)

                              acaCAATATGGAAACCCTCAACTCCAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTGGAAGGCa                                                                                                              <  5:727926/138‑1 (MQ=255)

                              aGACAATATGGAAACCCTCACCGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  2:1304248/140‑1 (MQ=255)

                              aGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGTCGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  4:118114/140‑1 (MQ=255)

                              aGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  3:947660/140‑1 (MQ=255)

                              aGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  5:760552/140‑1 (MQ=255)

                              aGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGGCGAAAAAGCCGATACCGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCa                                                                                                              <  7:130736/140‑1 (MQ=255)

                                  aaTATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGCCGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATc                                                                                                          >  4:337048/1‑140 (MQ=255)

                                  aaTATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATc                                                                                                          >  6:470215/1‑140 (MQ=255)

                                    tatGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCAc                                                                                                        >  3:1026645/1‑140 (MQ=255)

                                    tatGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCAc                                                                                                        >  2:1428722/1‑140 (MQ=255)

                                      tGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCg                                                                                                      >  2:1289027/1‑140 (MQ=255)

                                        taaaCCCTCAACTACAATTTAAATTTGTCCTAAAACGCCGATAACGCGGTGCAAGTAAAAGACGCTTTAAATAAAATGCGCGCGGCGGGGCGGTATGCGAAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGa                                                                                                    <  1:1070463/139‑1 (MQ=255)

                                        gAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGa                                                                                                    <  4:766610/140‑1 (MQ=255)

                                        gAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGa                                                                                                    <  3:838076/140‑1 (MQ=255)

                                        caaaCCCTCAACTACAATTTAAAAGTGGGCTAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGa                                                                                                    <  7:140524/139‑1 (MQ=255)

                                        aaaaCCCTCAACCAAAATTTAAACGTGGTCGAAAAAGCCGATAACGCGGTGCAAGACAACGACGCGTTAACTAAAATGCGCGCGGCGGCGCTGGATGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGa                                                                                                    <  1:14812/139‑1 (MQ=255)

                                         aaccccTCAACGACAATTTAAAAGTGTTCGAAAAAGCCGATAACGCTGTGAAAGTCAAAGACCCGTTAACTAAAATCCGCTCTGCGGCGCTGGATGAGCAAAAAGCGACGCCGCCGAAGCTTGAAGGAAAATCAACGGAc                                                                                                   >  5:198439/4‑140 (MQ=255)

                                           aCCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAg                                                                                                 >  2:989518/1‑140 (MQ=255)

                                           aCCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAg                                                                                                 >  6:197829/1‑140 (MQ=255)

                                            cccTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGc                                                                                                >  1:1019041/1‑140 (MQ=255)

                                            cccTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGc                                                                                                >  4:998546/1‑140 (MQ=255)

                                            cccTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGc                                                                                                >  4:295916/1‑140 (MQ=255)

                                             ccTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTAAAAGACGAGTTAACTAAAATGCGCGCTGCGGAGCTGTATGCGCAAAAAGCGAAGCAGCCGCAGCTTGCAGGCAAAACACCGGACAGcc                                                                                               >  4:1091119/1‑140 (MQ=255)

                                              cTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGccc                                                                                              >  1:229505/1‑140 (MQ=255)

                                               tCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCg                                                                                             >  3:707455/1‑140 (MQ=255)

                                                cAACGACAATTTAAAAGTGGTCTAAAAAGCCGATAACGCGCTGCAAGTCAAAGACGCGTTAACTAAAATGCTCGCTGCGGCGCAGGATGCGCAAAAAGCGCCGCCGCCTAATCTTGAAGGCAACTCACCGGACATCACgg                                                                                            >  7:847682/1‑140 (MQ=255)

                                                cAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCTACGCCGCCGCAGCTTGAAGGCAAATCACCGGACAGCCCgg                                                                                            >  2:712745/1‑140 (MQ=255)

                                                cAACGACAATTTAAAAGTGGCCGAAAAAGCCTAAACCGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGGGGTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCgg                                                                                            <  8:713743/140‑1 (MQ=255)

                                                      cAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGa                                                                                      >  1:1280219/1‑140 (MQ=255)

                                                      cAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGa                                                                                      >  6:1126302/1‑140 (MQ=255)

                                                      aaaTTTAAACGTGGTCGAAAAAGCCTATAACGCGGTGAAAGTCAAAGACGCTTTAACTAAAATGCGCGCTGCGGGGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGa                                                                                      <  5:367010/139‑1 (MQ=255)

                                                      aaaTTAAAACGTGGCCGAAAAACCCGATCACGCGGTGCAAGTCAACGCCGCGTTAACTAAAATGCGCGCGGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGa                                                                                      <  6:1281913/139‑1 (MQ=255)

                                                       aaTTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGaa                                                                                     >  4:593164/1‑140 (MQ=255)

                                                       aaTTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGTACAGCCCGTAAATGaa                                                                                     >  6:761278/1‑140 (MQ=255)

                                                       aaTTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCACAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGaa                                                                                     >  2:1417461/1‑140 (MQ=255)

                                                         tttAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAg                                                                                   >  2:461928/1‑140 (MQ=255)

                                                         tttAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGAAAATCACCGGACAGCCCTGAAATGAAAg                                                                                   >  6:1341879/1‑140 (MQ=255)

                                                                 tGGTCTAAAAAGCCGATACCGGGGTGCAATTCAAATACGCGTTAAATAAAATGCGCGCTGCGGGGCTGGAGGCGAAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGc                                                                           <  3:97486/140‑1 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAACATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCTCCa                                                                         >  4:391676/1‑140 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCTCTCGATGCGCAAAAAGCGACGCAGCCGAAGCTTGAAGGCAAATAACCGGACAGCCCGGAAATGAAAGACTTCCGcgc                                                                         >  5:534308/1‑138 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGCTGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGATATGAAAGACTTCCGCCa                                                                         >  5:35105/1‑140 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGccc                                                                         >  2:285668/1‑139 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         >  8:266928/1‑140 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         >  3:783288/1‑140 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         <  2:655180/140‑1 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCGGTATGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         <  1:719720/140‑1 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGAGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAAACACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         >  3:129813/1‑140 (MQ=255)

                                                                   gTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACGAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCa                                                                         >  5:498987/1‑140 (MQ=255)

                                                                      caaaaaGCCGATACCGCGGTCCAAGGCAACGACGCGTTAACTAAAAGGCGCGCTGCGGCGCTGGAGGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACgg                                                                      <  3:10623/139‑1 (MQ=255)

                                                                                    cgcgGTGCAAGTCAACTACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTgg                                                        <  5:575961/140‑1 (MQ=255)

                                                                                         tGCAAGTCAAAGACGCTTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTggtcggt                                                   <  8:1200465/140‑1 (MQ=255)

                                                                                         tGCAAGTCAAAGACGCTTTAAAAAAAATGCGCGCTGCGGCGCTGGATGCTCAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTggtcggt                                                   <  5:1089185/140‑1 (MQ=255)

                                                                                         tGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCGGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTggtcggt                                                   <  5:470215/140‑1 (MQ=255)

                                                                                         ggCAAGTCAACCCCGCGTTAACTAAAATGCGCGCTGCGGCGCGGGCTGCGCAAAAAGCGACGCCGCCGAAGATTTAAGTCAAATCACCGTACAGCCCGTAAATGAAAGACTTCCGCCACGGTTTCGACATTCTggtcggt                                                   <  8:1310029/139‑1 (MQ=255)

                                                                                                   aGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGATGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacg                                         >  8:820318/1‑140 (MQ=255)

                                                                                                   aGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacg                                         >  2:480450/1‑140 (MQ=255)

                                                                                                   aGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacg                                         >  7:505114/1‑140 (MQ=255)

                                                                                                   aGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacg                                         >  5:27813/1‑140 (MQ=255)

                                                                                                   aGACGCGTTAACTAAAATGCGCGCTGCGGCGCGGGATGCGCAAAATGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacg                                         <  4:147189/140‑1 (MQ=255)

                                                                                                    cccgcgTTAACTAAAATGCCGGCTGCGGCGCTGTAGGCCCAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGGCGGTCAGATTgacga                                        <  3:1194251/138‑1 (MQ=255)

                                                                                                    caagctttAACTAAATTGCGCGCTGCGGCGCTGGATGCTCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacga                                        <  5:702186/134‑1 (MQ=255)

                                                                                                    caCGCGTTAAATAAAATGCGCGCTGCGGCGCGGGATGCGAAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacga                                        <  1:554809/139‑1 (MQ=255)

                                                                                                       gcgTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGAcgc                                     >  1:872085/1‑140 (MQ=255)

                                                                                                        cgTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTgacga                                        <  3:252129/136‑1 (MQ=255)

                                                                                                         gTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGCCGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGCCTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACgcgc                                   >  6:714765/1‑140 (MQ=255)

                                                                                                                 aaaTGCGCGCTGCGGCGCTGGATGCGCAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGATAGACTTCGGCCACGGGTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTg                           >  5:1318018/1‑140 (MQ=255)

                                                                                                                   aTGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGc                         >  4:213008/1‑140 (MQ=255)

                                                                                                                       gcgcTGCGGCGCTGTATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAAt                     <  6:903152/140‑1 (MQ=255)

                                                                                                                            gcggcgCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAgg                <  4:1296951/140‑1 (MQ=255)

                                                                                                                             cggcgCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGCCGACGCGCTGAAGCTGGCAAATGAAGgt               >  3:591600/1‑140 (MQ=255)

                                                                                                                             cggcgCTGCATGCGCAAAAAGCTACGACGCCGAAGCTGGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCCCGGTTTCGACATTGTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAAGGAAGgt               <  6:532715/140‑1 (MQ=255)

                                                                                                                             cggcgCGGGTTGCGAAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGTAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGgt               <  7:742826/140‑1 (MQ=255)

                                                                                                                             cggcgCGGGAGGACCAAAAACCGACGACGCCGAAGCTTGAAGGCAAATACCCTGACAGCCCGGAAATGACATCCTTCCGCCACGGTTTCGACATTCTTGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGgt               <  7:579343/140‑1 (MQ=255)

                                                                                                                                  gtGGATGCGCAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGgtaaagt          <  2:1052106/139‑1 (MQ=255)

                                                                                                                                     gATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGgtaaagtaaa       >  8:40957/1‑140 (MQ=255)

                                                                                                                                     gATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGgtaaagtaaa       >  3:1393813/1‑140 (MQ=255)

                                                                                                                                     gATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCAGCCACGGTTTCGACAATCTGGTCGGTCAGATTGACGACGCGCTGAAGATGGCAAATGAAGgtaaagtaaa       >  1:1220877/1‑140 (MQ=255)

                                                                                                                                      atgttcAAAAAGCGCCGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTaaaa      <  1:1037159/135‑1 (MQ=255)

                                                                                                                                      aTGCGCAATAAGCGACGCGTCCGAAGCTGGATGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTTGTCGTTCAGATTTACGACGCGCTGAAGCTGGTAAATGAAGGTAAAGTaaaa      <  4:607044/140‑1 (MQ=255)

                                                                                                                                      aTGCGCAAAACGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTaaaa      <  4:1132588/140‑1 (MQ=255)

CAGTTCGGCGTCGTTTGCCGCTGATCTGGAAGACAATATGGAAACCCTCAACGACAATTTAAAAGTGGTCGAAAAAGCCGATAACGCGGTGCAAGTCAAAGACGCGTTAACTAAAATGCGCGCTGCGGCGCTGGATGCGCAAAAAGCGACGCCGCCGAAGCTTGAAGGCAAATCACCGGACAGCCCGGAAATGAAAGACTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAG  >  NZ_CP022686/3286743‑3287020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`