breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 56,326 C→A 12.3% E150* (GAA→TAA)  narH ← nitrate reductase subunit beta
RA NZ_CP022686 193,743 T→G 10.6% L309V (TTA→GTA)  rsxD → electron transport complex subunit RsxD
RA NZ_CP022686 211,868 T→A 8.2% Y119N (TAC→AAC)  gloA → lactoylglutathione lyase
RA NZ_CP022686 349,106 A→C 13.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 359,909 C→A 13.6% D20Y (GAT→TAT)  yoaG ← DUF1869 domain‑containing protein
RA NZ_CP022686 360,227 T→G 16.2% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,279 C→A 15.1% W17C (TGG→TGT yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 402,874 A→C 8.8% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,940 A→T 13.8% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 11.4% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 13.2% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 14.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 14.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 12.9% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 12.0% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 859,999 T→G 13.8% A620A (GCT→GCG yehM → DUF5682 family protein
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 15.0% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 16.5% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 10.7% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 15.7% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 8.3% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 12.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,008,537 C→A 6.5% intergenic (‑28/+175) inaA ← / ← glpQ lipopolysaccharide kinase InaA/glycerophosphodiester phosphodiesterase
RA NZ_CP022686 1,070,006 A→C 6.4% N176T (AAC→ACC)  pta → phosphate acetyltransferase
RA NZ_CP022686 1,100,944 T→G 16.3% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 18.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,304 A→T 8.7% intergenic (+33/‑11) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,147 T→G 13.4% L76R (CTG→CGG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,908 T→G 18.6% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,280,117 T→G 8.7% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,319,611 C→A 15.9% D325Y (GAT→TAT)  glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,360,709 T→G 11.1% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 15.3% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,504,305 A→T 10.3% N248Y (AAT→TAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 8.2% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,000 G→C 17.7% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,597,235 T→G 11.0% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,604,828 A→C 9.0% L390R (CTG→CGG)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,674,224 T→G 15.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 10.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 15.6% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.5% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,251 C→G 10.0% P179P (CCG→CCC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,273 C→G 8.4% C172S (TGC→TCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 11.7% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 15.8% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,828,110 A→C 7.1% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,833,756 T→G 21.7% Q464P (CAG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,779 T→G 23.3% K456N (AAA→AAC gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,782 C→A 11.4% V455V (GTG→GTT gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,070 C→A 13.8% E119* (GAA→TAA)  CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,602 T→G 12.7% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 9.9% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 2,027,390 A→C 7.9% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,047,987 A→C 7.9% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,148,109 T→G 8.8% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 10.3% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,179,878 T→G 7.3% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,179,916 G→T 11.5% R22R (CGC→CGA rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 7.7% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,797 G→T 10.5% intergenic (+39/‑58) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,272,831 G→T 6.9% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,489 T→G 18.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,393,031 C→A 6.5% T6N (ACC→AAC)  dctR → LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 21.4% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,295 G→T 12.1% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,429,953 A→C 8.3% R275R (CGT→CGG dctA ← C4‑dicarboxylate transporter DctC
RA NZ_CP022686 2,470,422 T→G 8.6% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,572,721 A→C 14.5% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 7.9% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,736,586 A→C 15.9% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.5% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,168 A→C 6.8% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,780,121 G→T 6.5% D681Y (GAT→TAT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,868,740 T→G 19.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.4% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,961,318 A→C 8.3% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,981,624 G→C 10.0% D571H (GAC→CAC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 9.8% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,229 T→G 11.7% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,281,004 T→G 9.4% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,062 T→G 9.4% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,345 G→T 11.1% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,229 T→G 15.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 14.8% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,504,054 T→G 8.9% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,528,197 A→C 8.0% N50T (AAC→ACC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,604,564 G→T 16.1% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,606,036 G→T 8.8% E250* (GAA→TAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,667,058 A→C 8.9% D5A (GAC→GCC)  mrcB → bifunctional glycosyl transferase/transpeptidase
RA NZ_CP022686 3,734,380 T→G 15.2% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 9.6% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,776,865 C→A 10.1% L123M (CTG→ATG)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 20.0% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,868,846 T→G 15.3% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,309 G→T 17.3% E163* (GAA→TAA)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 11.7% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 13.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 11.2% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.1% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,979,381 T→G 8.2% L96R (CTT→CGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,242 A→T 12.2% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,946 T→G 9.4% V132G (GTT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,214,114 A→T 10.2% intergenic (‑14/+24) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,435 G→C 9.6% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,287,784 T→G 9.8% intergenic (+15/‑34) CIW80_RS21490 → / → CIW80_RS21495 tRNA‑Lys/tRNA‑Lys
RA NZ_CP022686 4,357,188 T→G 5.5% G104G (GGT→GGG ompX → outer membrane protein OmpX
RA NZ_CP022686 4,368,341 T→G 13.7% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 11.4% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 8.7% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,427,625 T→A 5.8% intergenic (+90/‑9) yljB → / → clpS protein YljB/ATP‑dependent Clp protease adapter ClpS
RA NZ_CP022686 4,427,643 A→C 8.8% T4P (ACG→CCG)  clpS → ATP‑dependent Clp protease adapter ClpS
RA NZ_CP022686 4,440,324 C→A 15.4% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,491,111 A→C 6.6% R208R (CGT→CGG pncB ← nicotinate phosphoribosyltransferase
RA NZ_CP022686 4,620,262 A→C 7.5% intergenic (+6/+117) CIW80_RS23110 → / ← CIW80_RS26565 Exc2 family lipoprotein/hypothetical protein
RA NZ_CP022686 4,632,182 T→G 9.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 9.9% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,755,357 T→G 8.0% intergenic (+32/‑367) ycfP → / → ndh alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
RA NZ_CP022686 4,764,877 T→G 13.4% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,441 T→G 9.3% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,839,671 T→G 13.5% S149S (TCA→TCC minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 15.0% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,845,144 A→C 8.2% L101L (CTA→CTC umuD → translesion error‑prone DNA polymerase V autoproteolytic subunit
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,919,017 T→G 9.1% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,512 G→T 8.2% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,990,001 G→T 6.8% E353* (GAA→TAA)  ycjM → glucosylglycerate phosphorylase
RA NZ_CP022686 5,001,164 A→C 14.8% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,241 T→A 8.0% N624K (AAT→AAA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 9.4% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 6.9% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 100% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 395–2618 3050–2956 339–2656 4279 [4257] [4200] 4399 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1263–1318 3986–1723 406–2724 3228 [3040] [3164] 3226 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3743172 =235 (1.290)12 (0.070) 8/248 NT 5.0% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 219 (1.210)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase
* ? pMUT1‑hok‑empty 456 =2484 (0.480)3229 (0.620) 201/250 NT 65.1% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 977 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4913682 (0.710)2613 (0.630) 168/200 NT 57.2% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 977 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–