breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 197,859 G→T 10.8% A472S (GCA→TCA)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 228,613 A→T 13.7% D78E (GAT→GAA ydhT ← protein YdhT
RA NZ_CP022686 235,029 G→T 8.5% E15* (GAA→TAA)  pykF → pyruvate kinase PykF
RA NZ_CP022686 235,834 C→T 15.5% S283F (TCC→TTC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 235,864 C→T 5.6% A293V (GCA→GTA)  pykF → pyruvate kinase PykF
RA NZ_CP022686 349,092 A→C 8.7% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 372,574 A→C 17.0% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 418,374 T→G 13.7% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 494,360 Δ1 bp 5.1% coding (282/351 nt) flhD ← flagellar transcriptional regulator FlhD
RA NZ_CP022686 556,930 C→G 27.9% intergenic (+114/‑734) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,942 G→T 18.0% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 597,830 A→C 12.2% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 18.3% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 17.4% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 9.4% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 15.5% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,757 T→G 18.1% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 24.6% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 14.0% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 27.7% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.3% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,760 T→G 19.0% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 16.8% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 16.4% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 10.6% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 9.1% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
JC NZ_CP022686 1,061,329 Δ6 bp 11.6% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,065,997 A→C 10.9% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 12.4% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,145,575 C→A 11.8% E332* (GAA→TAA)  oxc ← oxalyl‑CoA decarboxylase
RA NZ_CP022686 1,197,204 T→G 5.0% V95G (GTA→GGA)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,935 A→C 16.5% V387G (GTG→GGG)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,360,709 T→G 9.9% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 10.1% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 8.7% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,277 T→G 8.3% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,370,559 T→G 15.9% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,392,487 A→C 9.5% T43P (ACG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase
RA NZ_CP022686 1,443,501 A→C 12.8% V20G (GTC→GGC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,460,615 A→C 15.7% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 12.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 11.7% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,355 T→A 12.8% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 17.5% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 13.6% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 11.9% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,246 C→G 7.8% G15A (GGC→GCC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,632,501 T→G 9.3% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 34.2% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 14.0% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 16.5% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.1% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,755 T→G 9.3% intergenic (‑365/‑290) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,705,974 T→G 14.5% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,860,070 C→A 14.8% E119* (GAA→TAA)  CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,925,332 A→C 9.6% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 15.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,964,332 A→C 7.4% G27G (GGT→GGG mug ← G/U mismatch‑specific DNA glycosylase
RA NZ_CP022686 1,965,173 A→C 8.7% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,175 T→G 8.2% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 8.6% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,044,069 T→G 5.8% R197R (CGT→CGG ubiU → peptidase U32 family protein
RA NZ_CP022686 2,049,089 T→G 19.1% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 13.4% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.8% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,785 A→C 11.1% E385A (GAG→GCG)  argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 10.3% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,556 C→G 7.2% intergenic (+26/‑207) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 8.6% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,461 A→T 8.1% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.2% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,236 A→C 8.2% G209G (GGT→GGG tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 8.3% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 17.5% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 12.2% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 12.4% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 5.1% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 8.8% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 10.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 7.5% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,098 T→G 7.2% F83V (TTT→GTT)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,357,291 A→C 11.1% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 19.8% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,493 A→T 7.7% L189F (TTA→TTT nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,393,013 T→G 6.1% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 13.5% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,410,581 A→C 6.5% pseudogene (2817/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,579 T→A 21.9% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,546,408 C→A 10.5% M90I (ATG→ATT waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,574,429 T→G 13.2% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,451 T→G 10.7% T223P (ACT→CCT)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,733,571 T→G 8.1% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,733,577 T→G 10.8% F55V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,743,563 T→G 9.4% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 11.6% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,138 A→C 11.0% T44P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 13.4% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 7.3% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,799,502 C→A 7.9% L82F (TTG→TTT CIW80_RS14015 ← tripartite tricarboxylate transporter permease
RA NZ_CP022686 2,801,864 T→G 6.9% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,868,753 T→G 12.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.1% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,920,428 A→C 9.3% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,218 C→A 10.2% E216* (GAA→TAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,981,597 G→T 14.3% E562* (GAA→TAA)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,612 A→C 7.4% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,625 A→C 8.6% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 10.3% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,013,752 T→G 10.7% V471G (GTG→GGG)  aceK → bifunctional isocitrate dehydrogenase kinase/phosphatase
RA NZ_CP022686 3,020,229 T→G 16.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,414 A→C 5.9% T195P (ACC→CCC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,136,940 T→G 7.6% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,225,762 A→C 6.6% L266F (TTA→TTC rnr → ribonuclease R
RA NZ_CP022686 3,252,190 T→G 13.5% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,363,064 C→A 6.6% intergenic (+763/‑28) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,199 T→A 9.9% intergenic (‑8/+27) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 13.4% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 10.1% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 14.5% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 12.7% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,224 T→G 15.8% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 17.7% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,493,370 T→G 5.9% K195N (AAA→AAC arcA ← two‑component system response regulator ArcA
RA NZ_CP022686 3,504,054 T→G 5.9% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,528,218 A→C 8.6% D57A (GAC→GCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,547,903 T→G 7.0% F15V (TTT→GTT)  CIW80_RS17770 → CcdB family protein
RA NZ_CP022686 3,625,704 T→G 8.8% R157R (CGT→CGG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,655,528 C→A 7.0% E769* (GAA→TAA)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 16.1% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 9.1% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 9.0% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,776,865 C→A 8.1% L123M (CTG→ATG)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 19.2% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 12.6% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,897,759 A→T 12.1% pseudogene (117/1083 nt) hyxB ← outer membrane autotransporter HyxB
RA NZ_CP022686 3,925,289 A→C 17.5% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 12.4% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,309 G→T 12.4% E163* (GAA→TAA)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 4,014,242 A→T 10.2% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,247 G→T 10.4% intergenic (+93/‑251) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 17.1% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,196,276 T→G 16.4% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,290,599 T→G 6.2% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,368,341 T→G 12.0% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 12.9% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 11.0% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,297 T→A 12.4% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 22.8% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,531,313 A→C 7.4% E62D (GAA→GAC hyaF → hydrogenase expression/formation protein
RA NZ_CP022686 4,571,459 G→T 12.7% F52L (TTC→TTA pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,632,182 T→G 8.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,712,763 A→C 16.3% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 13.8% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,061 T→C 16.0% H14R (CAC→CGC)  phoP ← two‑component system response regulator PhoP
RA NZ_CP022686 4,809,441 T→G 10.9% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 16.9% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,846 C→A 9.1% Q387H (CAG→CAT adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,545 A→C 14.0% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,157 G→T 17.7% D161Y (GAT→TAT)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→C 11.1% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→C 9.8% G1162G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.2% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 92.9% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–287 3085–2956 2670–2897 7916 [7335] [7670] 7795 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1263–1317 3987–1722 406–2725 3312 [3134] [3242] 3646 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =121 (0.660)87 (0.550) 47/216 NT 46.0% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 99 (0.620)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681118 (0.650)83 (0.520) 49/216 NT 45.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 99 (0.620)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =2567 (0.480)3603 (0.670) 213/248 NT 66.7% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1033 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4913638 (0.680)2959 (0.690) 169/198 NT 60.1% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1028 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–