breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 87,520 G→T 8.1% R217S (CGT→AGT)  yddA ← ABC transporter ATP‑binding protein/permease
RA NZ_CP022686 119,184 T→G 9.8% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 269,554 A→C 6.4% G269G (GGT→GGG selO ← protein adenylyltransferase SelO
RA NZ_CP022686 340,516 A→C 12.1% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 10.2% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 377,307 A→C 11.8% K147N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 410,041 G→C 11.1% Q365E (CAG→GAG)  zwf ← glucose‑6‑phosphate dehydrogenase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 524,842 T→G 11.0% V203G (GTC→GGC)  yedE → selenium metabolism membrane protein YedE/FdhT
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 18.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 11.5% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 693,001 A→C 6.8% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 693,005 A→C 10.8% L115L (CTA→CTC CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,391 C→A 9.1% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,402 G→T 8.9% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,406 G→T 14.8% intergenic (+166/‑30) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.0% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 10.5% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,756 C→G 14.1% D291H (GAC→CAC)  wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 777,337 A→C 9.7% R225R (CGT→CGG gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 777,365 A→C 12.0% L216R (CTG→CGG)  gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 828,846 A→C 16.4% L189R (CTT→CGT)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA NZ_CP022686 829,441 C→A 23.2% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 15.3% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,482 C→A 6.5% intergenic (‑71/+156) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,573 C→A 14.0% intergenic (‑146/+77) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 857,274 T→G 7.8% G78G (GGT→GGG yehL → MoxR family ATPase
RA NZ_CP022686 859,943 T→G 10.6% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 13.6% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 12.1% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,127 A→C 12.1% intergenic (+196/‑61) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 12.8% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,401 A→C 12.7% V955G (GTG→GGG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,000,431 T→G 29.8% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 12.1% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,419 T→A 16.9% intergenic (‑268/‑5) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,022,752 T→G 9.3% T44P (ACG→CCG)  yfaZ ← YfaZ family outer membrane protein
RA NZ_CP022686 1,064,076 A→C 6.8% T161P (ACG→CCG)  yfbR → 5'‑deoxynucleotidase
RA NZ_CP022686 1,065,978 A→C 8.5% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 9.6% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 10.5% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,197,147 T→G 9.8% L76R (CTG→CGG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,908 T→G 19.0% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,360,724 G→T 12.3% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,093 A→C 9.5% Y36S (TAC→TCC)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,410,626 T→G 9.2% V123G (GTC→GGC)  proW → glycine betaine/L‑proline ABC transporter permease ProW
RA NZ_CP022686 1,504,289 T→G 8.4% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,305 A→T 10.4% N248Y (AAT→TAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 15.4% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,323 C→A 16.9% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,339 C→A 19.2% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 10.4% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,518,282 T→G 9.1% T281P (ACC→CCC)  gudX ← enolase C‑terminal domain‑like protein
RA NZ_CP022686 1,535,319 T→G 14.1% M20R (ATG→AGG)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,546,981 T→A 7.6% intergenic (+3/‑31) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,546,996 T→A 15.2% intergenic (+18/‑16) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,000 G→C 15.0% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,003 G→C 11.4% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 12.5% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 11.1% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 8.1% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,203 A→C 10.3% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,209 A→C 11.6% K737T (AAG→ACG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,235 T→G 14.1% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,633,846 T→G 27.9% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,655,985 A→C 7.5% F221V (TTT→GTT)  prfB ← peptide chain release factor 2
RA NZ_CP022686 1,674,224 T→G 12.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.2% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 9.3% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 14.6% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 12.3% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 11.9% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,202 A→C 11.3% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,796,825 T→G 13.9% intergenic (+38/‑57) agn43 → / → CIW80_RS08945 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 1,832,023 T→G 10.4% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,749 G→T 15.8% V466V (GTC→GTA) ‡ gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,750 A→T 8.9% V466D (GTC→GAC) ‡ gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,602 T→G 8.3% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,965,159 A→C 9.2% L91R (CTG→CGG)  nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 1,975,137 A→C 8.6% intergenic (+6/‑57) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 2,009,589 A→C 7.6% L191R (CTT→CGT)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase
RA NZ_CP022686 2,016,170 A→C 7.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 10.3% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,037,497 A→C 6.5% S500R (AGC→CGC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 16.2% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 10.2% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,097 A→T 14.6% R253R (CGT→CGA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 7.8% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,070,812 A→C 7.3% intergenic (‑104/‑145) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,554 T→G 9.8% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 12.5% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 8.5% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 8.0% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,109 T→G 9.4% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 9.3% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.5% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 8.1% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,877 C→G 7.8% K35N (AAG→AAC rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,184,330 G→T 15.7% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,185,075 A→C 9.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,192,062 A→C 6.9% R69R (CGT→CGG rplD ← 50S ribosomal protein L4
RA NZ_CP022686 2,209,074 C→A 5.3% A258A (GCG→GCT chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,209,288 G→T 5.9% T187N (ACC→AAC)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,275,849 T→G 5.4% V73G (GTT→GGT)  feoA → ferrous iron transporter A
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,098 T→G 6.2% F83V (TTT→GTT)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,386,496 T→G 9.5% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,398,896 A→C 14.1% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,522 T→A 8.8% V19V (GTA→GTT yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,526 A→C 11.5% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 18.5% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,460,581 A→C 16.9% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,216 T→G 7.3% S542S (TCT→TCG upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,705 A→C 14.3% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 15.3% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 7.5% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,605,561 A→C 5.8% V153G (GTT→GGT)  nepI ← purine ribonucleoside efflux pump NepI
RA NZ_CP022686 2,628,001 T→G 7.0% S40R (AGC→CGC)  ibpB ← heat shock chaperone IbpB
RA NZ_CP022686 2,638,855 C→A 8.8% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,643,712 A→C 8.6% L413R (CTG→CGG)  dnaA ← chromosomal replication initiator protein DnaA
RA NZ_CP022686 2,703,299 A→C 7.3% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,743,565 C→G 8.6% noncoding (67/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,109 A→C 8.1% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,111 G→C 5.9% A35P (GCA→CCA)  yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 7.5% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 5.8% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,162 G→T 9.0% D52Y (GAT→TAT)  yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 6.5% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,801,817 T→G 7.1% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,127 T→G 11.4% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,810,014 A→C 5.4% S233S (TCA→TCC tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,834,886 A→C 9.0% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 8.9% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 19.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 13.8% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 10.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,920,385 C→A 6.3% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,989,627 T→G 7.4% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,229 T→G 10.2% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,080,461 T→G 10.7% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,114,640 A→C 5.5% G40G (GGT→GGG CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,114,653 A→C 6.4% V36G (GTC→GGC)  CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,136,923 A→C 8.5% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 9.7% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.6% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,218,260 T→G 7.6% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,265,084 T→G 17.7% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,278,016 G→C 8.4% D286H (GAC→CAC)  ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,281,004 T→G 11.8% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,359 A→T 6.0% intergenic (‑175/‑25) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 8.7% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,374 C→G 6.2% intergenic (‑190/‑10) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,064 C→A 9.4% intergenic (+763/‑28) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,387,116 A→C 9.5% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,387,650 A→C 6.6% V220G (GTC→GGC)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.8% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,215 A→T 15.7% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 11.7% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 16.3% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 10.4% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,496,452 A→C 13.0% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,516,069 T→G 6.7% V211G (GTT→GGT)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,526,230 G→T 8.7% A209S (GCC→TCC)  dapB → 4‑hydroxy‑tetrahydrodipicolinate reductase
RA NZ_CP022686 3,625,683 T→G 12.2% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,634,591 G→T 10.0% D260Y (GAT→TAT)  acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,634,614 A→C 9.9% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 7.4% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 11.8% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,703,648 A→C 7.4% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 17.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,509 A→T 9.5% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 10.8% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 9.8% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 14.0% L130V (TTA→GTA) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,483 A→T 7.5% L130F (TTA→TTT) ‡ CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 10.0% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,265 T→A 7.4% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,868,846 T→G 7.6% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,935,324 T→G 10.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 10.8% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 8.7% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,949,972 T→G 7.2% N140T (AAC→ACC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,950,302 T→G 5.4% D30A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 6.7% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 15.6% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 3,984,841 T→G 7.4% R67R (CGT→CGG queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA
RA NZ_CP022686 4,014,238 C→G 14.2% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 7.7% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 14.2% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,033,367 T→G 5.0% V342G (GTT→GGT)  amtB → ammonium transporter AmtB
RA NZ_CP022686 4,056,962 C→A 20.0% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,074,902 G→T 9.3% S23* (TCG→TAG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,079,363 A→C 8.6% A158A (GCT→GCG ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,133,839 T→G 15.6% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,188,933 C→A 6.9% G325C (GGC→TGC)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,188,944 A→C 6.7% V321G (GTA→GGA)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,213,260 T→G 7.4% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,213,261 A→T 7.1% M189L (ATG→TTG)  ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,280,091 T→G 8.8% V102G (GTC→GGC)  cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,290,599 T→G 6.2% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,905 A→C 6.3% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 13.9% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 8.9% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,297 T→A 9.6% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 19.3% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 11.2% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,620,262 A→C 7.8% intergenic (+6/+117) CIW80_RS23110 → / ← CIW80_RS26565 Exc2 family lipoprotein/hypothetical protein
RA NZ_CP022686 4,632,182 T→G 5.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,947 G→C 6.4% E731Q (GAA→CAA)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,642,951 A→C 7.0% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,041 A→C 17.6% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 24.3% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 17.8% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,762,516 T→G 12.6% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,764,877 T→G 14.2% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 7.2% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,889 T→G 7.7% intergenic (‑69/‑192) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 11.9% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,814,990 T→G 19.1% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,826,160 T→G 6.4% K250N (AAA→AAC sitA ← iron/manganese ABC transporter substrate‑binding protein SitA
RA NZ_CP022686 4,839,687 T→G 13.7% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,695 A→T 15.4% I141I (ATT→ATA minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,852,202 A→C 7.2% N149T (AAC→ACC)  dadA → D‑amino acid dehydrogenase
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 10.8% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,098 C→G 6.7% noncoding (6/132 nt) CIW80_RS24835 ← RtT sRNA
RA NZ_CP022686 4,916,118 G→T 6.6% intergenic (+245/+40) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,951,521 A→C 10.1% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,041,191 G→A 8.1% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 17.2% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,149 T→C 5.5% G1260G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 6.9% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 1,349 G→T 18.1% intergenic (–/+368)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,369 G→T 8.3% intergenic (–/+348)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,375 Δ1 bp 5.1% intergenic (–/+342)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,376 Δ1 bp 92.3% intergenic (–/+341)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,379 G→T 7.2% intergenic (–/+338)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,407 G→T 7.6% intergenic (–/+310)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,411 C→A 9.3% intergenic (–/+306)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,413 T→G 6.7% intergenic (–/+304)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,423 G→T 10.1% intergenic (–/+294)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,431 G→T 10.4% intergenic (–/+286)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 2,084 C→A 44.3% intergenic (‑50/‑107) putative HTH domain protein ← / → uncharacterized protein –/–
RA pMUT1‑hok‑empty 4,123:1 +T 94.2% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 404–2619 3047–2957 339–2644 6552 [5857] [6290] 6523 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_MW240712 4 =0 (0.000)90 (0.010)
+CAC
43/244 NT 100% intergenic (–/+203) –/JNG15_RS00005 –/helix‑turn‑helix domain‑containing protein
?NZ_MW240712 = 3173 0 (0.000)intergenic (+182/–) mobA/– plasmid mobilization protein MobA/–
* ? pMUT1‑hok‑empty 456 =3923 (0.450)5412 (0.620) 233/250 NT 66.3% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1577 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4915888 (0.680)4534 (0.660) 179/200 NT 59.1% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1578 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–