breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
JC NZ_CP022686 235,533 Δ11 bp 15.2% coding (547‑557/1413 nt) pykF → pyruvate kinase PykF
RA NZ_CP022686 340,516 A→C 9.6% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 360,227 T→G 19.9% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 7.9% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 9.4% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 16.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 694,391 C→A 12.1% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 741,905 A→C 10.1% E261D (GAA→GAC hisD → histidinol dehydrogenase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 25.0% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 13.1% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 20.7% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 878,883 T→G 8.2% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 881,760 T→G 18.7% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,051 G→T 12.2% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 14.8% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 14.6% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,100,944 T→G 14.0% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,100,959 T→G 13.4% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,112,973 A→C 7.1% V127G (GTG→GGG)  fadJ ← fatty acid oxidation complex subunit alpha FadJ
RA NZ_CP022686 1,174,272 A→C 17.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,431 C→A 8.9% intergenic (+41/‑6) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 18.4% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,462 A→C 26.2% L426R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,918 C→A 12.2% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,237,929 C→A 7.5% L299F (TTG→TTT tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,319,588 T→G 13.0% E332D (GAA→GAC glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,360,724 G→T 8.2% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 15.7% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,479,631 G→C 7.3% intergenic (‑40/+153) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,328 G→T 13.9% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 9.7% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,632,525 T→G 6.9% V174G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,710,453 A→C 9.9% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,167 A→C 15.5% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 6.8% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,805,047 G→T 14.3% pseudogene (328/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,832,023 T→G 8.7% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,833,745 T→G 19.9% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 2,016,170 A→C 9.8% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,502 G→T 10.2% P501P (CCG→CCT lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 18.7% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,568 C→G 8.7% intergenic (+38/‑195) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 9.9% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 10.2% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,102 C→G 21.9% L122V (CTT→GTT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,109 T→G 13.0% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,114 C→G 17.9% L126V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.6% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 8.6% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 8.6% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,271,374 A→C 7.6% G227G (GGT→GGG ompR ← two‑component system response regulator OmpR
RA NZ_CP022686 2,310,451 C→A 8.3% E241* (GAA→TAA)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 10.5% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 17.8% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,386,496 T→G 11.9% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,394,295 G→T 8.3% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,522 T→A 9.0% V19V (GTA→GTT yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,521,024 T→G 6.1% V229G (GTT→GGT)  lldP → L‑lactate permease
RA NZ_CP022686 2,546,394 A→C 11.3% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,420 T→A 12.7% G86G (GGA→GGT waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,423 A→C 14.3% V85V (GTT→GTG waaG ← glycosyltransferase family 4 protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,677,646 T→G 7.6% K82N (AAA→AAC atpD ← F0F1 ATP synthase subunit beta
RA NZ_CP022686 2,736,585 T→A 11.7% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 18.0% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 7.8% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 7.6% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,157 T→G 7.9% V50G (GTG→GGG)  yifL → lipoprotein
RA NZ_CP022686 2,769,155 A→C 7.9% S61A (TCC→GCC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,868,740 T→G 24.7% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 16.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,449 T→G 5.9% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,915 T→G 8.4% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 3,020,229 T→G 8.4% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,252,190 T→G 9.7% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,265,084 T→G 17.1% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,004 T→G 9.5% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,044 T→G 14.3% L88R (CTG→CGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 13.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,229 T→G 16.3% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,625,683 T→G 11.6% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,628,882 A→C 8.2% D324A (GAC→GCC)  aceF → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
RA NZ_CP022686 3,652,102 A→T 7.7% intergenic (‑111/+1) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,731,466 T→C 60.3% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 65.5% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 31.2% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 7.3% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 14.3% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,932,456 A→C 9.4% C179W (TGT→TGG lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 11.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 6.6% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,631 A→C 12.5% H80P (CAC→CCC)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 12.0% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,291 G→T 25.3% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 19.4% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,078,994 C→A 13.6% intergenic (‑28/+33) cnoX ← / ← ybbO chaperedoxin/NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,331,751 T→G 11.1% T352P (ACG→CCG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,440,334 A→C 18.9% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,562,550 T→G 15.4% N140H (AAC→CAC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,587,091 T→G 13.7% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,712,985 A→C 10.0% intergenic (‑67/+67) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,755,357 T→G 7.0% intergenic (+32/‑367) ycfP → / → ndh alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
RA NZ_CP022686 4,764,877 T→G 10.5% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,221 T→G 15.4% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 5,043,788 G→A 5.9% S1140N (AGT→AAT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 8.5% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,614 T→C 7.9% G1415G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.6% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 1,316:1 +C 11.8% intergenic (–/+401)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,317:1 +C 9.1% intergenic (–/+400)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,376 Δ1 bp 100% intergenic (–/+341)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,407 G→T 9.9% intergenic (–/+310)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,415 C→A 6.5% intergenic (–/+302)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,423 G→T 12.9% intergenic (–/+294)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,430 G→T 12.8% intergenic (–/+287)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 4,123:1 +T 95.0% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3084–2957 340–2896 11864 [10911] [10700] 11525 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 = 1761697NA (NA)13 (0.080) 10/250 NT 7.5% intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
?NZ_CP022686 1866488 = 161 (0.960)coding (86/1500 nt) pitB inorganic phosphate transporter PitB
* ? NZ_CP022686 3899666 =76 (0.450)108 (0.740) 45/218 NT 60.0% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 78 (0.530)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 389968178 (0.470)99 (0.680) 50/218 NT 57.6% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 78 (0.530)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_MW240712 3 =0 (0.000)31 (0.000)
+TC
21/246 NT 100% intergenic (–/+204) –/JNG15_RS00005 –/helix‑turn‑helix domain‑containing protein
?NZ_MW240712 = 3173 0 (0.000)intergenic (+182/–) mobA/– plasmid mobilization protein MobA/–
* ? pMUT1‑hok‑empty 456 =3775 (0.450)5313 (0.630) 217/250 NT 66.3% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1631 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4915527 (0.660)4172 (0.620) 175/200 NT 58.0% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1626 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–