breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 47,354 G→T 11.0% P665P (CCG→CCT vgrG → type VI secretion system tip protein TssI/VgrG
RA NZ_CP022686 119,184 T→G 10.5% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 238,317 G→T 20.2% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,324 G→C 17.7% V85V (GTC→GTG sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 11.5% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 9.3% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,092 A→C 10.4% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,106 A→C 13.2% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,123 A→C 10.6% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 360,246 T→G 13.4% E28D (GAA→GAC yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 16.8% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,135 A→C 10.3% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 8.8% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 16.8% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.4% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,713 A→C 8.0% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 636,764 G→C 7.4% V435V (GTC→GTG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 694,391 C→A 13.8% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 15.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 19.8% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 22.6% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,733 A→C 14.4% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 21.0% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 22.6% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 12.9% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 13.0% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 973,436 A→C 5.1% D253A (GAC→GCC)  rcsD → phosphotransferase RcsD
RA NZ_CP022686 1,005,073 A→C 11.9% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 14.0% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,098 G→C 6.5% D385H (GAC→CAC)  nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,396 G→T 9.9% intergenic (‑245/‑28) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,011,419 T→A 8.7% intergenic (‑268/‑5) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,034,493 T→G 8.7% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,065,982 G→T 8.5% V35V (GTC→GTA yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 11.7% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 15.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,203,718 T→G 6.8% R239R (CGT→CGG hemF → oxygen‑dependent coproporphyrinogen oxidase
RA NZ_CP022686 1,209,908 T→G 19.4% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 10.4% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,269,551 T→G 6.6% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,280,117 T→G 7.1% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,290,935 A→C 13.2% S734R (AGT→AGG yfhM ← alpha2‑macroglobulin
RA NZ_CP022686 1,360,724 G→T 8.3% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 13.4% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 15.4% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,424,362 A→T 8.1% intergenic (‑58/+5) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,479,595 G→C 9.1% intergenic (‑4/+189) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,634 G→C 10.9% intergenic (‑43/+150) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 12.4% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,328 G→T 13.9% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,353 G→C 7.1% A264P (GCT→CCT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,354 C→A 12.2% A264D (GCT→GAT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,373 G→C 8.9% K270N (AAG→AAC CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 9.4% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,573 T→G 11.7% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,604,869 G→C 7.3% G376G (GGC→GGG lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,619,264 C→A 6.1% intergenic (+53/+264) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,508 C→A 10.5% I168I (ATC→ATA arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 30.8% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,244 T→G 15.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 10.1% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,251 C→G 8.1% P179P (CCG→CCC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,260 A→T 9.2% F176L (TTT→TTA ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,011 G→T 10.4% intergenic (‑621/‑34) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,833,779 T→G 15.8% K456N (AAA→AAC gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,799 T→G 11.7% T450P (ACG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,602 T→G 12.9% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,317 T→G 6.3% L146R (CTG→CGG)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,332 A→C 9.6% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 18.0% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,049,089 T→G 21.0% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 11.8% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 11.0% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.4% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,193 A→C 5.9% V606G (GTT→GGT)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,554 T→G 7.2% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 14.8% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 10.0% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,127,686 C→G 6.0% E45Q (GAA→CAA)  yhdP ← AsmA2 domain‑containing protein YhdP
RA NZ_CP022686 2,130,829 A→C 10.3% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 11.0% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 16.6% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 12.1% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 12.3% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,150,071 T→G 8.0% G290G (GGT→GGG prmA → 50S ribosomal protein L11 methyltransferase
RA NZ_CP022686 2,172,177 A→C 6.0% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,075 A→C 8.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,198 G→C 5.6% G43G (GGC→GGG tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 21.3% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 11.1% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,380,936 A→C 10.0% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,394,287 T→G 19.8% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,885 A→C 8.9% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,581 A→C 23.3% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,216 T→C 8.5% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,577 A→G 9.7% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,705 A→C 7.1% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 9.6% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,703,299 A→C 9.8% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,718,627 T→G 6.8% F510V (TTC→GTC)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,719,166 A→T 6.3% I122I (ATT→ATA ilvY ← HTH‑type transcriptional activator IlvY
RA NZ_CP022686 2,736,578 C→A 8.4% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 18.5% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 11.7% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,613 C→A 15.8% intergenic (+28/‑15) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,801,864 T→G 7.2% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,127 T→G 12.9% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,808,145 T→G 5.8% V447G (GTG→GGG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,834,886 A→C 10.2% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 19.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 9.2% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 7.9% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 10.4% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,919,074 T→G 7.1% D340A (GAC→GCC)  CIW80_RS14605 ← hypothetical protein
RA NZ_CP022686 2,920,428 A→C 9.5% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,994,693 T→G 8.7% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,017,622 A→C 19.6% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,070,986 T→G 12.6% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,080,461 T→G 8.1% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,086,834 A→C 8.4% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,136,940 T→G 8.7% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,948 G→T 8.8% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 20.4% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,297,360 T→G 8.4% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 9.2% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,616 C→G 8.5% A143P (GCC→CCC)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,459,370 C→A 9.4% S62Y (TCT→TAT)  bglJ → DNA‑binding transcriptional activator BglJ
RA NZ_CP022686 3,461,200 C→A 13.7% intergenic (‑9/+26) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 13.6% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 10.2% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 22.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,528,197 A→C 7.1% N50T (AAC→ACC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,528,218 A→C 9.2% D57A (GAC→GCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,570,252 T→G 5.9% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,625,683 T→G 12.3% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,628,523 T→A 6.0% A204A (GCT→GCA aceF → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
RA NZ_CP022686 3,652,079 T→G 8.8% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,657,084 T→G 6.2% Y250S (TAC→TCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,665,943 A→C 11.2% K474N (AAA→AAC hrpB → ATP‑dependent helicase HrpB
RA NZ_CP022686 3,734,380 T→G 17.4% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,739,795 T→G 6.6% noncoding (70/77 nt) CIW80_RS18670 → tRNA‑Asp
RA NZ_CP022686 3,770,524 T→G 6.4% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 20.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,266 A→C 10.6% Y1586S (TAC→TCC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,852,459 T→G 11.9% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 10.7% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,857 T→G 8.5% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,279 T→A 9.5% Y153N (TAC→AAC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 16.8% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 9.2% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 10.0% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 8.5% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.9% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 11.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 4,004,135 T→G 11.4% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,238 C→G 13.8% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,018,222 C→A 9.3% intergenic (+157/‑31) clpX → / → lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
RA NZ_CP022686 4,056,951 C→A 15.4% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 19.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,214,457 G→T 14.8% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,234,427 C→A 10.1% intergenic (+127/‑315) glnS → / → chiP glutamine‑‑tRNA ligase/chitoporin
RA NZ_CP022686 4,368,341 T→G 10.6% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 10.3% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,334 A→T 17.5% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,445,534 T→G 8.6% intergenic (+40/‑199) serS → / → dmsA serine‑‑tRNA ligase/dimethylsulfoxide reductase subunit A
RA NZ_CP022686 4,535,349 A→C 10.3% T118T (ACA→ACC appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,571,417 T→G 8.5% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,459 G→T 11.3% F52L (TTC→TTA pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,589,367 T→G 7.9% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,589,371 C→A 9.7% L245L (CTG→CTT CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,605,593 T→G 15.2% Q51P (CAG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 10.6% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,041 A→C 17.8% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 23.6% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 10.6% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 7.8% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 12.7% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 18.7% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,809,441 T→G 9.2% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,909,248 A→T 7.7% intergenic (‑14/+20) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,916,069 C→A 6.4% intergenic (+196/+89) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,951,998 T→G 6.8% S198A (TCG→GCG)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,043,648 G→C 8.2% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.9% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 1,373 T→G 12.8% intergenic (–/+344)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,376 Δ1 bp 100% intergenic (–/+341)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,392 T→G 6.4% intergenic (–/+325)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,407 G→T 11.9% intergenic (–/+310)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,409 T→G 7.5% intergenic (–/+308)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,413 T→G 7.6% intergenic (–/+304)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,423 G→T 7.3% intergenic (–/+294)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,430 G→T 15.2% intergenic (–/+287)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,441 G→T 9.4% intergenic (–/+276)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,449 C→A 9.4% intergenic (–/+268)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 2,078:1 +A 38.9% intergenic (‑44/‑113) putative HTH domain protein ← / → uncharacterized protein –/–
RA pMUT1‑hok‑empty 4,123:1 +T 94.6% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 682–2618 3043–2957 340–2362 6726 [6681] [6647] 7008 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3899666 =271 (1.260)17 (0.090) 12/218 NT 7.1% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 208 (1.110)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681260 (1.210)28 (0.150) 18/218 NT 11.4% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 208 (1.110)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =4262 (0.420)6350 (0.630) 199/250 NT 67.6% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1822 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4916396 (0.640)5028 (0.620) 162/200 NT 59.2% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1821 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–