breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 10.4% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 349,096 C→A 10.5% A603A (GCC→GCA yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 13.9% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,236 C→A 19.4% D32Y (GAT→TAT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,290 T→G 12.4% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 10.4% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 512,726 C→A 20.5% intergenic (‑226/+4) sdiA ← / ← yecC transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 531,408 A→C 13.0% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,942 G→T 15.1% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 12.2% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 19.2% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 17.7% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 10.0% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 11.5% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,016 C→A 5.6% P119Q (CCA→CAA)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,016 C→A 12.8% V35F (GTT→TTT)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,757 T→G 14.9% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 24.7% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 22.4% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.6% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 13.7% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 857,274 T→G 7.6% G78G (GGT→GGG yehL → MoxR family ATPase
RA NZ_CP022686 881,733 A→C 17.6% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 23.1% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 13.1% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 11.0% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 17.4% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,047,883 T→G 8.8% intergenic (‑113/+51) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,100,944 T→G 16.3% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,269,557 C→A 10.8% T1122T (ACG→ACT CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,353,233 A→C 21.6% V72G (GTT→GGT)  yfiF ← tRNA/rRNA methyltransferase
RA NZ_CP022686 1,360,709 T→G 11.3% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 12.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,727 A→T 10.2% intergenic (‑62/+262) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 16.0% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,559 T→G 19.3% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,372,477 C→A 5.4% T13T (ACC→ACA pheL → pheA operon leader peptide PheL
RA NZ_CP022686 1,479,607 A→C 10.1% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 9.6% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,535,365 A→C 21.5% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,546,988 T→A 9.1% intergenic (+10/‑24) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,003 G→C 13.5% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 13.9% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 12.0% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 8.1% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 35.2% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 18.8% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,231 A→T 8.1% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 15.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.3% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,692,094 G→T 10.4% I174I (ATC→ATA CIW80_RS08330 ← nucleoside/nucleotide kinase family protein
RA NZ_CP022686 1,706,025 C→A 17.5% intergenic (‑635/‑20) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 12.8% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,202 A→C 10.4% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 6.6% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,785,695 T→G 8.4% pseudogene (16/186 nt) CIW80_RS08875 ← zinc‑ribbon domain‑containing protein
RA NZ_CP022686 1,833,799 T→G 13.6% T450P (ACG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,865,103 A→C 8.7% L491V (TTG→GTG)  pitB ← inorganic phosphate transporter PitB
RA NZ_CP022686 1,911,602 T→G 11.0% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 10.7% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 9.8% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 6.5% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 17.5% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 11.2% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,097 A→T 12.9% R253R (CGT→CGA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 7.3% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,110,465 T→G 10.1% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,105 C→G 19.9% L123V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,118 C→G 12.9% A127G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 6.6% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,184,330 G→T 10.6% F64L (TTC→TTA rplO ← 50S ribosomal protein L15
RA NZ_CP022686 2,209,288 G→T 5.6% T187N (ACC→AAC)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,797 G→T 11.0% intergenic (+39/‑58) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 13.9% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 20.8% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,972 T→G 8.1% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,380,922 A→C 13.7% S182R (AGC→CGC)  pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,380,936 A→C 10.3% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,394,287 T→G 16.9% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,410,581 A→C 10.8% pseudogene (2817/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,470,422 T→G 12.1% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,558,864 T→G 5.5% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,721 A→C 14.6% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 10.7% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,736,585 T→A 7.7% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 15.1% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 11.1% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,868,740 T→G 17.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.7% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,903,361 T→G 8.8% N205T (AAC→ACC)  hslU ← HslU‑‑HslV peptidase ATPase subunit
RA NZ_CP022686 2,904,856 T→G 8.0% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 6.6% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,977,481 T→G 6.5% V558G (GTA→GGA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA NZ_CP022686 2,981,625 A→C 10.2% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,983,975 T→G 5.9% G1354G (GGT→GGG rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 3,136,940 T→G 12.3% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,184,639 A→C 8.8% intergenic (‑86/+30) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.2% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,281,062 T→G 10.9% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,317,665 A→T 13.8% intergenic (+83/+36) lptG → / ← yjgR LPS export ABC transporter permease LptG/DUF853 domain‑containing protein
RA NZ_CP022686 3,334,291 C→A 6.5% G250C (GGT→TGT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,348,334 T→G 6.7% intergenic (‑150/‑50) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,614 G→T 8.5% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,625 C→A 11.2% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,215 A→T 13.1% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 12.0% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,225 T→G 7.7% intergenic (‑34/+1) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 19.0% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 11.2% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,504,054 T→G 9.8% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,625,683 T→G 11.5% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,652,090 T→G 10.4% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,540 A→C 8.9% F765V (TTT→GTT)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,714,342 T→G 9.8% L275V (TTG→GTG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,776,865 C→A 8.8% L123M (CTG→ATG)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 10.1% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,895,534 T→G 8.8% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 10.4% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 14.2% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,368 C→G 6.3% G284G (GGC→GGG prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 18.5% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 9.0% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 14.6% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,004,135 T→G 8.7% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,238 C→G 12.5% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,951 C→A 13.7% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,074,902 G→T 13.2% S23* (TCG→TAG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,133,839 T→G 14.4% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,133,850 T→G 12.1% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,214,439 G→C 8.8% intergenic (‑12/+23) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,457 G→T 10.8% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,270,824 A→C 9.6% K490T (AAG→ACG)  sucA → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,331,940 A→C 17.7% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,335,504 T→G 8.5% K58N (AAA→AAC ybhF ← ATP‑binding cassette domain‑containing protein
RA NZ_CP022686 4,440,324 C→A 15.3% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 17.5% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,560,980 A→C 6.5% V663G (GTT→GGT)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,571,417 T→G 11.3% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 9.7% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,593 T→G 9.8% Q51P (CAG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 10.1% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 12.3% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,759,393 A→C 5.7% V213G (GTA→GGA)  ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,762,516 T→G 9.6% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,787,799 C→A 8.4% intergenic (‑52/‑44) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,804,059 A→C 9.8% E620A (GAG→GCG)  CIW80_RS24225 → phage terminase large subunit family protein
RA NZ_CP022686 4,809,441 T→G 11.5% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,967 C→G 6.9% A63G (GCT→GGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,981 T→G 10.2% W68G (TGG→GGG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,015 A→T 6.5% Y79F (TAC→TTC)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,687 T→G 12.9% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,709 C→G 8.2% D137H (GAC→CAC)  minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,846 C→A 10.3% Q387H (CAG→CAT adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,919,017 T→G 6.5% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,512 G→T 16.1% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,951,521 A→C 10.8% T39P (ACC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,043,558 T→A 9.4% S1063R (AGT→AGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 94.0% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–316 3085–2957 2642–2897 9505 [8752] [9328] 9480 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1265–1318 3983–1722 405–2719 3873 [3824] [3833] 3913 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 3743172 =248 (1.370)14 (0.080) 11/248 NT 5.3% intergenic (+1/+253) CIW80_RS18695/CIW80_RS18700 IS66 family transposase/IS110‑like element ISEc45 family transposase
?NZ_CP022686 = 3744618 251 (1.400)intergenic (‑60/‑4) CIW80_RS18700/CIW80_RS18705 IS110‑like element ISEc45 family transposase/transposase
* ? pMUT1‑hok‑empty 456 =3225 (0.480)4317 (0.650) 215/250 NT 66.1% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1207 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4914653 (0.700)3425 (0.640) 169/200 NT 58.2% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1206 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–