breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA NZ_CP022686 457,895 Δ1 bp pseudogene (287/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
MC JC NZ_CP022686 628,478 Δ1,286 bp [clbP] [clbP]
RA NZ_CP022686 756,115 A→G G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 917,427 +TT intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 2,214,419 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,408,862 Δ1 bp pseudogene (1098/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
JC NZ_CP022686 2,590,564 +TGGT coding (110/153 nt) CIW80_RS26455 → hypothetical protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,424,795 A→C intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
MC JC NZ_CP022686 3,559,837 Δ2,343 bp coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,656,188 A→G S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,792,805 A→G Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp coding (2‑1037/1056 nt) dinB → DNA polymerase IV
MC JC NZ_CP022686 4,844,868 Δ1,662 bp [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,914 Δ1 bp intergenic (‑110/‑118) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA pHH‑29_RBS 1,649 Δ1 bp coding (151/570 nt) Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 380–2617 3062–2957 341–2683 2443 [2253] [2355] 2387 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2777–2841 5510–3245 405–2734 1781 [1741] [1532] 1856 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =0 (0.000)155 (0.950) 66/270 0.3 100% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.010)124 (0.760) 45/270 1.3 99.2% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase