breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 340,453 A→C 12.9% L52V (TTG→GTG)  ydjK ← MFS transporter
RA NZ_CP022686 349,092 A→C 13.8% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 26.5% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 20.1% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.3% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,727 A→C 12.7% L448V (TTG→GTG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 694,391 C→A 11.8% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 26.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 21.1% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 1,005,085 C→A 19.1% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,237,918 C→A 10.2% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,353,558 T→A 5.6% intergenic (‑111/‑96) yfiF ← / → trxC tRNA/rRNA methyltransferase/thioredoxin TrxC
RA NZ_CP022686 1,373,199 G→C 67.3% T205T (ACG→ACC pheA → bifunctional chorismate mutase/prephenate dehydratase
RA NZ_CP022686 1,674,248 T→G 16.8% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,860,077 G→T 13.9% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,925,332 A→C 12.2% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 13.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 10.3% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,070,788 A→C 11.0% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,380,936 A→C 12.9% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 14.0% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,516,577 A→G 11.8% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,705 A→C 14.1% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 11.0% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,736,578 C→A 10.8% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 11.8% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,109 A→C 6.8% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,868,740 T→G 17.5% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,423,638 A→C 9.8% L373V (TTG→GTG)  yjiJ ← MFS transporter
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,634,614 A→C 13.8% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 19.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 18.1% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,925,289 A→C 13.2% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 4,301,920 T→G 9.5% V136G (GTT→GGT)  modB → molybdate ABC transporter permease subunit
RA NZ_CP022686 4,440,334 A→T 22.7% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,571,439 T→G 13.6% H59P (CAC→CCC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,643,107 T→G 6.6% V784G (GTT→GGT)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,839,687 T→G 13.2% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 188–2618 3086–2957 340–2899 6695 [6506] [6494] 6982 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2773–2841 5512–3104 264–2740 3152 [3135] [2931] 3431 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =2 (0.020)114 (1.040) 58/246 NT 98.3% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624682 (0.020)123 (1.120) 57/246 NT 98.4% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 = 38444251 (0.010)87 (0.800) 54/244 NT 97.8% coding (1/1056 nt) dinB DNA polymerase IV
?NZ_CP022686 3845462 = 3 (0.030)coding (1038/1056 nt) dinB DNA polymerase IV