breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 238,317 G→T 26.5% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 9.9% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 372,574 A→C 15.5% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 379,600 T→G 9.9% intergenic (+35/‑20) manZ → / → yobD PTS mannose transporter subunit IID/DUF986 family protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 12.0% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 556,942 G→T 20.2% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 23.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,299 C→G 14.0% C1608W (TGC→TGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 31.8% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 9.9% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 13.2% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,008 A→T 13.7% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 24.6% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 29.8% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 18.6% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 17.7% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,513 C→A 13.5% intergenic (‑102/+125) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 20.2% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 975,262 A→C 6.0% T862P (ACG→CCG)  rcsD → phosphotransferase RcsD
RA NZ_CP022686 1,005,085 C→A 12.6% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,174,272 A→C 16.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,416 A→T 9.9% intergenic (+26/‑21) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,360,709 T→G 12.3% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 11.1% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 10.2% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 19.5% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,465,015 A→C 9.0% N178H (AAC→CAC)  pphB → protein‑serine/threonine phosphatase
RA NZ_CP022686 1,564,569 A→C 13.8% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 8.6% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,640,888 G→T 10.8% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 18.2% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 9.4% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 8.7% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 12.6% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,775,873 G→T 8.4% H189N (CAT→AAT)  CIW80_RS08815 ← N‑acetylneuraminate epimerase
RA NZ_CP022686 1,889,761 A→C 6.9% V459G (GTT→GGT)  ygiQ ← YgiQ family radical SAM protein
RA NZ_CP022686 1,911,602 T→G 11.1% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,923 T→G 17.6% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 11.1% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 14.9% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,804 A→C 9.8% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,465 T→G 7.7% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 11.0% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 8.4% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,159,673 C→A 11.5% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,179,877 C→G 10.0% K35N (AAG→AAC rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,050 A→C 9.3% R54R (CGT→CGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 9.4% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 13.0% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 22.1% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,535 A→C 11.9% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 25.5% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 11.7% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 11.7% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,400 A→C 14.2% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,558,864 T→G 6.4% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,736,578 C→A 9.2% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 11.2% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,821,651 A→C 8.2% intergenic (+107/‑271) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 8.4% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 8.7% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 25.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 9.4% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 9.7% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 19.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,874,073 A→C 8.0% T62P (ACC→CCC)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,904,852 T→G 12.5% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,417 G→C 10.5% A65P (GCA→CCA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 3,086,834 A→C 8.0% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,297,329 A→T 15.1% V826V (GTA→GTT mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,297,360 T→G 13.7% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,390,604 C→G 11.5% D147H (GAC→CAC)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.9% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,229 T→G 21.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,528,214 A→C 9.5% T56P (ACC→CCC)  carB → carbamoyl‑phosphate synthase large subunit
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,570,252 T→G 11.2% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,634,614 A→C 12.0% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 12.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,682,246 A→C 15.2% D268A (GAC→GCC)  dgt → dGTPase
RA NZ_CP022686 3,714,300 T→G 9.0% W261G (TGG→GGG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,734,367 T→G 18.2% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 11.7% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 6.7% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 9.5% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,798,081 A→T 8.0% I330F (ATC→TTC)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 9.7% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,925,304 A→C 14.0% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,376 T→G 10.0% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 10.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,697 A→C 11.9% V317G (GTG→GGG)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,707 A→C 12.8% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 6.9% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 7.7% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,408 T→G 7.9% V105G (GTT→GGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,238 C→G 14.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 21.6% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 10.5% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,133,850 T→G 9.0% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,270,799 A→C 12.1% T482P (ACG→CCG)  sucA → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,286,483 T→G 6.9% R47R (CGT→CGG cpoB → cell division protein CpoB
RA NZ_CP022686 4,440,334 A→C 18.6% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 10.1% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,632,182 T→G 10.3% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,674,512 T→G 5.9% V278G (GTC→GGC)  CIW80_RS23440 → GTPase family protein
RA NZ_CP022686 4,678,041 A→C 16.4% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,759,393 A→C 9.9% V213G (GTA→GGA)  ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,764,877 T→G 20.4% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 9.8% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 8.4% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 10.2% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,437 T→G 10.7% L49R (CTG→CGG)  CIW80_RS24260 → head‑tail joining protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,159 C→A 20.2% F95L (TTC→TTA CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 5,042,304 T→C 11.1% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 8.5% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
JC pHH‑29_RBS 1,780 Δ11 bp 100% coding (282‑292/570 nt) Aldafermin (NGM282) →
RA pHH‑29_RBS 2,872 G→T 12.8% intergenic (‑451/+257) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,899 Δ1 bp 100% intergenic (‑478/+230) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,930 G→T 8.0% intergenic (‑509/+199) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,934 C→A 8.7% intergenic (‑513/+195) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,947 G→T 8.7% intergenic (‑526/+182) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,953 G→T 13.6% intergenic (‑532/+176) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,954 G→T 9.3% intergenic (‑533/+175) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 380–2618 3062–2957 340–2683 8986 [8289] [8350] 8458 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =3 (0.020)148 (0.930) 66/248 NT 98.0% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.020)162 (1.020) 64/248 NT 98.2% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =129 (0.810)36 (0.260) 28/216 NT 22.7% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 133 (0.960)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681125 (0.790)25 (0.180) 22/216 NT 17.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 133 (0.960)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA