breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 28,266 T→G 7.3% V19G (GTT→GGT)  ydcV → ABC transporter permease
RA NZ_CP022686 182,646 A→C 8.6% G68G (GGT→GGG malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 263,831 A→C 10.0% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 15.9% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,123 A→C 11.5% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 372,512 T→A 6.6% S254S (TCT→TCA sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 512,298 A→C 8.4% V68G (GTT→GGT)  sdiA ← transcriptional regulator SdiA
RA NZ_CP022686 512,735 G→T 11.1% Q250K (CAA→AAA)  yecC ← L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 531,408 A→C 10.6% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,940 A→T 13.8% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,948 A→T 8.8% intergenic (+132/‑716) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 10.9% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 15.0% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 23.2% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 11.9% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,421 G→T 12.0% intergenic (+181/‑15) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,001 A→T 8.0% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 23.0% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 14.7% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 859,943 T→G 14.4% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 881,733 A→C 13.7% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 17.5% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,051 G→T 12.5% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 12.3% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 11.5% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,000,431 T→G 21.6% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,042 A→C 7.2% D366A (GAC→GCC)  nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,073 A→C 8.8% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,010,174 G→C 5.6% V326V (GTC→GTG glpT ← glycerol‑3‑phosphate transporter
RA NZ_CP022686 1,065,974 G→C 7.9% A38G (GCA→GGA)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,209,908 T→G 15.1% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,475 A→C 6.7% C422G (TGT→GGT)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,213,479 T→G 13.1% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,242,396 A→C 7.7% S111A (TCG→GCG)  dapA ← 4‑hydroxy‑tetrahydrodipicolinate synthase
RA NZ_CP022686 1,280,117 T→G 8.6% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,308,537 G→T 6.4% G32C (GGT→TGT)  yphA → DoxX family protein
RA NZ_CP022686 1,358,687 T→G 7.9% L348V (TTA→GTA)  pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA NZ_CP022686 1,360,724 G→T 12.0% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,742 C→A 7.4% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,277 T→G 6.5% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,370,559 T→G 9.3% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,093 A→C 11.6% Y36S (TAC→TCC)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,410,835 T→G 5.4% F193V (TTT→GTT)  proW → glycine betaine/L‑proline ABC transporter permease ProW
RA NZ_CP022686 1,424,198 A→T 5.8% intergenic (‑34/+30) CIW80_RS07080 ← / ← CIW80_RS07085 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,424,347 A→T 6.5% intergenic (‑43/+20) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,479,607 A→C 11.4% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 7.9% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,324 C→A 16.1% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 10.5% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 11.4% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 26.5% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,326 T→G 15.5% V22V (GTT→GTG fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,362 G→T 17.4% A34A (GCG→GCT fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,110 G→C 17.2% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 13.1% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,566,203 A→C 9.4% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,271 A→C 8.9% T758P (ACC→CCC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 10.4% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,861 A→T 17.9% L248* (TTA→TAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,901 T→G 7.5% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 17.1% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 10.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 16.6% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.9% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,710,453 A→C 7.8% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,145 A→C 8.7% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 14.6% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,798,974 G→T 6.2% G8C (GGT→TGT)  CIW80_RS08965 → DUF932 domain‑containing protein
RA NZ_CP022686 1,833,745 T→G 23.3% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,860,077 G→T 14.1% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,581 C→G 8.7% S231T (AGC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,317 T→G 8.3% L146R (CTG→CGG)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 20.4% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,173 A→C 8.4% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,153 T→G 6.1% noncoding (121/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,035,513 T→A 5.5% Y141F (TAT→TTT)  rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,037,521 A→C 11.4% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 17.8% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,780 A→C 6.5% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 10.1% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,097,310 A→C 6.4% T245P (ACC→CCC)  gltB → glutamate synthase large subunit
RA NZ_CP022686 2,110,465 T→G 9.5% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,234 T→A 6.7% Y210F (TAT→TTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 11.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 11.3% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 10.3% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,688 A→C 9.2% Y172S (TAC→TCC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 5.8% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 9.4% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,071 A→C 5.6% G47G (GGT→GGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 7.8% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 8.7% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,194,054 G→T 9.1% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 12.7% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,386,496 T→G 8.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,394,287 T→G 17.0% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,295 G→T 7.5% I112I (ATC→ATA chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,302 A→T 11.7% F110Y (TTC→TAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,885 A→C 9.9% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 10.8% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,406,317 G→T 5.6% I69I (ATC→ATA CIW80_RS12130 ← hypothetical protein
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,433,335 A→C 8.5% S1057A (TCG→GCG)  bcsC ← cellulose synthase complex outer membrane protein BcsC
RA NZ_CP022686 2,460,581 A→C 26.0% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 13.6% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 8.6% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,550,305 T→G 6.7% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
RA NZ_CP022686 2,572,705 A→C 14.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 9.8% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,607,374 G→T 8.7% S232Y (TCC→TAC)  adeQ ← adenine permease AdeQ
RA NZ_CP022686 2,638,855 C→A 8.5% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,717,385 T→G 5.2% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,585 T→A 8.6% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 9.5% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,758,168 A→C 7.9% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 12.4% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,780,127 C→A 11.6% P683T (CCA→ACA)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,808,145 T→G 7.0% V447G (GTG→GGG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,810,040 A→C 6.5% N242T (AAC→ACC)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,834,898 A→C 7.9% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 24.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 14.1% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 8.3% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 8.7% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 6.6% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,919,096 A→C 8.0% C333G (TGT→GGT)  CIW80_RS14605 ← hypothetical protein
RA NZ_CP022686 2,920,417 G→C 8.7% A65P (GCA→CCA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,933,091 G→T 6.6% R339L (CGT→CTT)  frwC → PTS fructose transporter subunit EIIC
RA NZ_CP022686 2,937,239 T→G 5.9% T209P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,989,627 T→G 10.5% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,992,831 A→C 10.3% K133T (AAA→ACA)  nfi → deoxyribonuclease V
RA NZ_CP022686 3,020,229 T→G 11.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,986 T→G 7.9% G53G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,086,834 A→C 10.5% F708V (TTT→GTT)  uvrA ← excinuclease ABC subunit UvrA
RA NZ_CP022686 3,090,033 G→T 6.0% R32S (CGT→AGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,923 A→C 12.1% S167A (TCG→GCG)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,935 T→G 8.5% S163R (AGC→CGC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 11.9% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,142,411 T→G 5.2% D59A (GAC→GCC)  phnH ← phosphonate C‑P lyase system protein PhnH
RA NZ_CP022686 3,184,639 A→C 7.2% intergenic (‑86/+30) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
RA NZ_CP022686 3,184,651 A→C 9.6% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 6.0% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,218,260 T→G 8.1% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,265,096 G→T 13.5% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,004 T→G 12.1% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,334,291 C→A 7.1% G250C (GGT→TGT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,348,357 T→G 8.4% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,393 C→A 5.1% R4S (CGC→AGC)  CIW80_RS26495 → hypothetical protein
RA NZ_CP022686 3,387,644 A→C 9.7% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,671 T→G 7.4% K124N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,411,278 C→A 6.9% S93Y (TCC→TAC)  fimH → type 1 fimbria D‑mannose specific adhesin FimH
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,215 A→T 11.9% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 13.4% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 19.1% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,504,054 T→G 7.9% V204G (GTT→GGT)  tal → transaldolase
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 14.2% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 12.4% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,661,525 T→A 6.7% N7Y (AAT→TAT)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,703,648 A→C 7.6% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 23.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 12.7% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,776,865 C→A 6.0% L123M (CTG→ATG)  CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 17.6% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,228 T→A 6.7% N1573K (AAT→AAA cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,806,236 G→T 9.2% R1576I (AGA→ATA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,806,262 G→T 9.0% D1585Y (GAT→TAT)  cdiA → contact‑dependent inhibition effector tRNA nuclease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 11.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,516 A→C 9.1% intergenic (‑122/+62) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,895,534 T→G 8.8% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,914,747 A→C 9.3% D132A (GAC→GCC)  yahG → DUF1116 domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 14.3% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 10.5% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 8.7% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 12.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,685 G→C 7.7% S321C (TCC→TGC)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 12.7% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 8.6% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,950,011 T→G 6.1% H127P (CAC→CCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 4,004,135 T→G 6.8% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,271 G→T 15.5% intergenic (+117/‑227) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,018,267 T→G 5.5% R5R (CGT→CGG lon → endopeptidase La
RA NZ_CP022686 4,032,292 A→C 14.9% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,041,478 A→C 5.9% V768G (GTG→GGG)  acrB ← efflux RND transporter permease AcrB
RA NZ_CP022686 4,052,690 A→C 5.3% T408P (ACC→CCC)  dnaX → DNA polymerase III subunit gamma/tau
RA NZ_CP022686 4,056,962 C→A 14.9% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 11.8% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,331,751 T→G 13.1% T352P (ACG→CCG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,929 C→A 13.9% M292I (ATG→ATT ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 12.2% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 8.9% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 15.0% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 20.2% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,351 T→A 11.8% V803D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,732 T→G 9.8% intergenic (+86/‑84) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,485,614 T→G 11.3% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,571,414 T→G 8.8% P67P (CCA→CCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,571,417 T→G 9.9% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,632,182 T→G 9.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,224 T→G 5.7% C290W (TGT→TGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,041 A→C 19.7% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 23.5% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 13.3% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 7.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,506 T→G 8.5% V72G (GTT→GGT)  CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,981 T→G 6.4% W68G (TGG→GGG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,047 T→G 6.1% S90A (TCG→GCG)  CIW80_RS24295 → phage tail protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,221 T→G 19.4% L116R (CTT→CGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,910,213 A→C 9.0% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,918,837 T→G 5.9% K390N (AAA→AAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,943,295 T→G 7.1% K5N (AAA→AAC trpE ← anthranilate synthase component I
RA NZ_CP022686 4,951,545 A→C 11.5% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,164 A→C 10.8% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→G 11.3% N645K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,416 G→C 12.5% S1016T (AGC→ACC)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 1,534 G→A 15.1% W12* (TGG→TGA Aldafermin (NGM282) →
JC pHH‑29_RBS 1,669 (GCGGTCAATCTGCCCACAGCTTATTAGAAATTAAA)1→2 55.1% coding (171/570 nt) Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 357–2618 3068–2957 340–2712 9186 [9139] [9086] 10134 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2773–2894 5510–3245 352–2738 6174 [6132] [5205] 6149 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =1 (0.000)227 (1.020) 74/250 NT 99.6% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.000)209 (0.940) 78/250 NT 99.5% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 1522881 =NA (NA)1841 (0.380) 179/250 NT 21.3% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pHH‑29_RBS 1521 = 6794 (0.730)coding (23/570 nt) Aldafermin (NGM282)
* ? NZ_CP022686 1760448 =NA (NA)2219 (0.460) 206/250 NT 24.9% coding (1099/1101 nt) CIW80_RS08705 IS30‑like element IS30 family transposase
?pHH‑29_RBS = 1523 6693 (0.710)coding (25/570 nt) Aldafermin (NGM282)