breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 9.4% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 238,323 A→C 17.3% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 6.7% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 350,006 A→C 11.1% E224D (GAA→GAC yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 17.6% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 379,600 T→G 6.9% intergenic (+35/‑20) manZ → / → yobD PTS mannose transporter subunit IID/DUF986 family protein
RA NZ_CP022686 418,379 T→G 5.7% G49G (GGT→GGG znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 482,325 T→G 12.5% E180A (GAG→GCG)  flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 496,086 A→C 9.3% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 496,135 A→C 7.4% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 512,717 T→G 19.1% intergenic (‑217/+13) sdiA ← / ← yecC transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 524,842 T→G 14.6% V203G (GTC→GGC)  yedE → selenium metabolism membrane protein YedE/FdhT
RA NZ_CP022686 556,942 G→T 16.7% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 19.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.7% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 694,391 C→A 14.3% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,406 G→T 15.5% intergenic (+166/‑30) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 776,084 A→C 5.4% G268G (GGT→GGG fcl ← GDP‑L‑fucose synthase
RA NZ_CP022686 780,653 C→A 13.6% A372A (GCG→GCT wcaC ← colanic acid biosynthesis glycosyltransferase WcaC
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 26.6% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 23.5% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 864,173 T→G 15.8% intergenic (+39/+2) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 881,739 A→C 24.8% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 21.3% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 15.6% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 13.4% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,509 G→C 6.7% A43G (GCC→GGC)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 954,516 A→C 10.6% L41V (TTG→GTG)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 12.1% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,431 T→G 27.2% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 10.3% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,098 G→C 8.9% D385H (GAC→CAC)  nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,417 G→T 13.0% intergenic (‑266/‑7) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,022,718 T→G 5.2% N55T (AAC→ACC)  yfaZ ← YfaZ family outer membrane protein
RA NZ_CP022686 1,100,944 T→G 15.0% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 13.3% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,416 A→T 8.4% intergenic (+26/‑21) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 18.7% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 11.9% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,215,721 A→C 8.0% L258V (TTA→GTA)  pta ← phosphate acetyltransferase
RA NZ_CP022686 1,239,837 T→G 10.3% intergenic (‑134/+34) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,239,865 G→T 17.1% intergenic (‑162/+6) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,276,970 A→C 5.8% G414G (GGT→GGG der ← ribosome biogenesis GTPase Der
RA NZ_CP022686 1,280,117 T→G 7.4% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,292,615 A→C 5.2% G174G (GGT→GGG yfhM ← alpha2‑macroglobulin
RA NZ_CP022686 1,360,724 G→T 10.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 16.9% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,392,487 A→C 6.3% T43P (ACG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase
RA NZ_CP022686 1,396,420 T→G 6.7% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,424,198 A→T 5.0% intergenic (‑34/+30) CIW80_RS07080 ← / ← CIW80_RS07085 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,424,362 A→T 10.9% intergenic (‑58/+5) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,441,800 T→G 7.0% N587T (AAC→ACC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,479,607 A→C 8.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,625 G→C 8.9% intergenic (‑34/+159) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,258 G→T 13.8% S232I (AGT→ATT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,313 C→G 5.0% C250W (TGC→TGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,323 C→A 13.4% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 9.7% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 25.2% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,536,472 A→C 6.2% Q404H (CAA→CAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,000 G→C 14.9% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 18.2% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 7.9% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,543 A→C 6.7% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,203 A→C 9.1% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,217 A→C 13.1% T740P (ACG→CCG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,480 T→G 8.9% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,632,501 T→G 9.1% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 28.6% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 15.9% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 15.6% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 9.7% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 6.9% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.6% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,263 G→T 6.3% T175T (ACC→ACA ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,692,094 G→T 7.4% I174I (ATC→ATA CIW80_RS08330 ← nucleoside/nucleotide kinase family protein
RA NZ_CP022686 1,716,167 A→C 10.6% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,730,020 C→A 7.7% R43S (CGT→AGT)  CIW80_RS08540 → integrase arm‑type DNA‑binding domain‑containing protein
RA NZ_CP022686 1,758,771 T→G 8.9% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,828,110 A→C 8.9% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,832,023 T→G 7.8% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,860,077 G→T 18.3% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,911,594 C→A 9.0% D227Y (GAT→TAT)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 11.2% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 15.3% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,971,460 T→G 7.5% intergenic (+51/‑133) ebgR → / → ebgA transcriptional regulator EbgR/beta‑galactosidase subunit alpha
RA NZ_CP022686 2,016,170 A→C 9.3% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 5.1% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,049,089 T→G 13.7% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,780 A→C 7.0% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 12.1% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 17.0% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 7.9% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 6.7% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 10.4% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 11.3% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 13.4% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 8.1% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 12.1% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 11.2% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,176 T→G 6.8% G146G (GGT→GGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,071 A→C 6.7% G47G (GGT→GGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 7.2% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,199,460 T→G 5.3% R379R (CGT→CGG gspE → type II secretion system ATPase GspE
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 9.5% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,310,429 T→G 7.2% N248T (AAC→ACC)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,489 T→G 16.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 17.2% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,291 C→A 10.5% E114* (GAA→TAA)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,535 A→C 10.7% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,573 G→T 14.5% I2I (ATC→ATA yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 13.1% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,499,319 T→G 6.5% N44T (AAC→ACC)  aldB ← aldehyde dehydrogenase AldB
RA NZ_CP022686 2,546,394 A→C 11.1% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 6.4% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,703,299 A→C 8.0% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,717,400 T→G 6.1% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,573 G→C 7.7% E179Q (GAA→CAA)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 8.2% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,758,128 A→C 7.0% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 7.4% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 15.3% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,801,864 T→G 7.8% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,834,886 A→C 9.1% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,753 T→G 14.1% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,761 T→G 7.8% N15T (AAC→ACC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,874,073 A→C 7.1% T62P (ACC→CCC)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,904,856 T→G 7.7% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,981,612 A→C 8.6% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,625 A→C 6.5% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 3,017,622 A→C 12.2% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,188 C→A 11.3% F119L (TTC→TTA yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,229 T→G 11.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,136,940 T→G 8.5% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,137,023 A→C 8.5% G133G (GGT→GGG phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,950 A→C 6.1% Q252H (CAA→CAC rdcB → YjcZ‑like family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.8% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,218,260 T→G 9.0% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,239,387 T→G 7.4% V396G (GTC→GGC)  ulaA → PTS ascorbate transporter subunit IIC
RA NZ_CP022686 3,297,333 T→G 9.5% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,345 G→T 6.4% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 5.9% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,671 T→G 8.0% K124N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 14.5% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,834 C→A 10.6% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 20.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,516,069 T→G 6.4% V211G (GTT→GGT)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,529,239 A→C 5.4% K397N (AAA→AAC carB → carbamoyl‑phosphate synthase large subunit
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,570,252 T→G 8.6% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,604,565 A→T 17.1% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 12.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 7.2% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 20.7% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 19.2% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 11.6% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,979 A→C 7.5% pseudogene (1255/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,800,981 A→C 7.3% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,228 T→A 8.1% N1573K (AAT→AAA cdiA → contact‑dependent inhibition effector tRNA nuclease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 10.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,279 T→A 7.8% Y153N (TAC→AAC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 9.2% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 11.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 9.4% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 13.7% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 7.0% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,949,969 T→G 8.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,611 A→C 8.8% E73D (GAA→GAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,628 T→A 9.8% L79* (TTA→TAA)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,640 A→C 5.9% Q83P (CAG→CCG)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 8.5% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 4,014,238 C→G 19.0% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 11.1% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,248 C→G 10.5% intergenic (+94/‑250) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 22.1% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 26.1% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,213,266 A→T 6.7% L190F (TTA→TTT ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,234 C→A 6.7% intergenic (‑22/+27) CIW80_RS21085 ← / ← CIW80_RS21090 tRNA‑Gln/tRNA‑Met
RA NZ_CP022686 4,214,457 G→T 9.7% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,631 T→A 7.9% noncoding (14/85 nt) CIW80_RS21105 ← tRNA‑Leu
RA NZ_CP022686 4,287,532 G→T 5.9% noncoding (45/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,290,635 T→G 5.3% T109P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,940 A→C 13.8% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 10.3% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 15.5% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 13.0% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,334 A→C 15.3% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 9.4% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,285 A→C 8.6% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,571,414 T→G 8.2% P67P (CCA→CCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,605,579 T→G 12.1% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,616,703 T→G 13.9% intergenic (+185/+348) CIW80_RS23075 → / ← CIW80_RS26560 hypothetical protein/ATP‑binding protein
RA NZ_CP022686 4,632,182 T→G 10.8% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,954 A→C 7.2% Q733P (CAG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,047 A→C 24.0% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,759,393 A→C 8.0% V213G (GTA→GGA)  ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,759,441 A→C 6.8% V197G (GTT→GGT)  ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,764,877 T→G 15.8% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 12.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 11.4% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 13.7% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,787,836 A→C 7.3% intergenic (‑89/‑7) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,814,981 T→G 6.5% W68G (TGG→GGG)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 11.8% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 11.1% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,815,883 T→G 8.0% V24G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,815,886 T→G 9.6% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,839,668 T→G 20.0% V150V (GTA→GTC minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 16.9% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,909,248 A→T 8.7% intergenic (‑14/+20) CIW80_RS24840 ← / ← CIW80_RS24845 tRNA‑Tyr/tRNA‑Tyr
RA NZ_CP022686 4,951,512 G→T 8.6% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,951,538 C→A 18.3% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 4,951,548 G→C 14.0% A48P (GCC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,952,020 T→G 8.9% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,974,199 T→G 5.8% R169R (CGT→CGG CIW80_RS25180 → efflux RND transporter permease subunit
RA NZ_CP022686 4,997,085 A→C 7.7% N158T (AAC→ACC)  ycjT → kojibiose phosphorylase
RA NZ_CP022686 5,001,157 G→T 14.3% D161Y (GAT→TAT)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,073 A→G 5.0% A568A (GCA→GCG CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 9.0% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→C 9.3% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.4% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 1,534 G→A 15.0% W12* (TGG→TGA Aldafermin (NGM282) →
RA pHH‑29_RBS 1,674:1 +A 78.3% coding (176/570 nt) Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 717–2618 3020–2957 340–2304 4199 [4125] [4163] 4235 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2771–2897 5512–3104 208–2742 6777 [6429] [6559] 7143 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =0 (0.000)222 (0.960) 81/250 NT 100% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624680 (0.000)187 (0.810) 76/250 NT 100% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =193 (0.830)94 (0.470) 55/218 NT 36.6% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 158 (0.780)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681185 (0.800)99 (0.490) 52/218 NT 38.3% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 158 (0.780)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA