breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 46,589 T→G 8.2% R410R (CGT→CGG vgrG → type VI secretion system tip protein TssI/VgrG
RA NZ_CP022686 119,184 T→G 9.9% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 192,380 T→A 9.3% R597R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 228,009 T→G 8.4% L531R (CTT→CGT)  ydhS → FAD‑NAD(P)‑binding protein
RA NZ_CP022686 249,241 A→C 7.6% T352P (ACG→CCG)  ydiK → AI‑2E family transporter YdiK
RA NZ_CP022686 263,831 A→C 10.5% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 349,106 A→C 10.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 15.3% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 16.1% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 487,568 T→G 7.3% K523N (AAA→AAC tar ← methyl‑accepting chemotaxis protein II
RA NZ_CP022686 496,086 A→C 9.9% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 498,662 A→C 13.3% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 512,735 G→T 20.8% Q250K (CAA→AAA)  yecC ← L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 531,408 A→C 14.2% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 22.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 24.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,845 C→A 5.4% L239F (TTG→TTT clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 651,848 A→C 13.1% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 667,110 T→G 5.9% K3080T (AAG→ACG)  clbB ← colibactin hybrid non‑ribosomal peptide synthetase/type I polyketide synthase ClbB
RA NZ_CP022686 684,606 T→G 7.7% intergenic (‑967/‑213) CIW80_RS26355 ← / → CIW80_RS03520 IS4 family transposase/TonB‑dependent receptor
RA NZ_CP022686 694,388 C→A 9.4% intergenic (+148/‑48) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,438 G→T 13.5% M1M (ATG→ATT) † CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 9.8% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 768,242 T→G 6.1% intergenic (‑616/+46) wcaK ← / ← wzxC colanic acid biosynthesis pyruvyl transferase WcaK/colanic acid undecaprenyl disphosphate flippase WzxC
RA NZ_CP022686 777,337 A→C 6.9% R225R (CGT→CGG gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 20.9% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 20.3% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 864,166 A→C 13.1% intergenic (+32/+9) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 864,173 T→G 17.5% intergenic (+39/+2) yehR → / ← yehS YehR family lipoprotein/DUF1456 family protein
RA NZ_CP022686 878,883 T→G 9.1% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 954,712 T→G 6.5% Y134S (TAC→TCC)  ccmE ← cytochrome c maturation protein CcmE
RA NZ_CP022686 996,762 T→G 7.7% D579A (GAC→GCC)  gyrA ← DNA topoisomerase (ATP‑hydrolyzing) subunit A
RA NZ_CP022686 998,355 A→C 5.6% V48G (GTA→GGA)  gyrA ← DNA topoisomerase (ATP‑hydrolyzing) subunit A
RA NZ_CP022686 1,005,085 C→A 11.9% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,034,493 T→G 9.0% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,064,076 A→C 7.9% T161P (ACG→CCG)  yfbR → 5'‑deoxynucleotidase
RA NZ_CP022686 1,100,944 T→G 16.7% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,197,146 C→G 7.4% L76V (CTG→GTG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,908 T→G 14.3% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,483 T→G 13.6% N419T (AAC→ACC)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,239,837 T→G 13.3% intergenic (‑134/+34) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,269,551 T→G 7.9% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,278,783 T→G 8.0% D242A (GAC→GCC)  bamB ← outer membrane protein assembly factor BamB
RA NZ_CP022686 1,319,588 T→G 15.7% E332D (GAA→GAC glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,360,724 G→T 8.6% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,441,772 A→C 6.6% R596R (CGT→CGG hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,479,607 A→C 9.1% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,323 C→A 10.6% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 21.6% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,000 G→C 11.7% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 13.5% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 10.4% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,246 C→G 7.5% G15A (GGC→GCC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,633,846 T→G 31.2% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 12.3% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 14.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 10.2% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,167 A→C 13.3% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,832,023 T→G 9.0% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,925,365 A→C 8.3% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 22.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,175 T→G 9.3% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,047,692 A→C 5.5% F41V (TTC→GTC)  mtr ← tryptophan permease
RA NZ_CP022686 2,048,050 A→C 5.6% R602R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 18.6% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,193 A→C 8.0% V606G (GTT→GGT)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,554 T→G 9.9% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 15.4% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 9.2% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 6.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,159,660 G→T 13.4% D163Y (GAT→TAT)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,159,687 T→A 11.1% Y172N (TAC→AAC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 5.5% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,172,741 A→C 6.4% L265V (TTA→GTA)  dprA ← DNA‑protecting protein DprA
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,356,231 T→G 8.6% D9A (GAC→GCC)  tusA ← sulfurtransferase TusA
RA NZ_CP022686 2,357,291 A→C 10.1% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,380,922 A→C 12.7% S182R (AGC→CGC)  pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 6.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,397,370 T→G 9.9% F9C (TTT→TGT)  chuT → heme ABC transporter substrate‑binding protein ChuT
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,516,216 T→C 6.7% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,577 A→G 7.8% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,521,024 T→G 8.9% V229G (GTT→GGT)  lldP → L‑lactate permease
RA NZ_CP022686 2,558,864 T→G 8.0% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,705 A→C 10.6% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 13.7% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 10.4% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,717,407 T→G 8.4% V103G (GTG→GGG)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,780,149 T→G 6.0% V690G (GTC→GGC)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,801,864 T→G 8.3% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,808,135 C→A 9.3% P444T (CCT→ACT)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,834,886 A→C 9.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,837,432 C→A 7.4% intergenic (+73/‑46) CIW80_RS26475 → / → yihI hypothetical protein/Der GTPase‑activating protein YihI
RA NZ_CP022686 2,868,740 T→G 19.8% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 9.1% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,856 T→G 8.8% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,908,932 A→C 5.8% F36V (TTT→GTT)  priA ← primosomal protein N'
RA NZ_CP022686 2,920,408 C→A 8.1% Q62K (CAA→AAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,418 C→A 6.6% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,428 A→C 7.9% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,989,627 T→G 8.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,080,461 T→G 8.9% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,090,099 G→C 7.6% L10V (CTT→GTT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,090,101 A→C 7.5% V9G (GTT→GGT)  yjcB ← YjcB family protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,089 C→A 18.0% E75D (GAG→GAT ytfJ ← YtfJ family protein
RA NZ_CP022686 3,327,608 C→A 7.2% intergenic (+166/‑872) CIW80_RS16560 → / → CIW80_RS16565 helix‑turn‑helix transcriptional regulator/hypothetical protein
RA NZ_CP022686 3,348,342 A→T 6.9% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,605 T→G 20.0% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,845 A→C 10.7% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,229 T→G 18.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,528,197 A→C 8.1% N50T (AAC→ACC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,528,214 A→C 7.1% T56P (ACC→CCC)  carB → carbamoyl‑phosphate synthase large subunit
RA NZ_CP022686 3,528,218 A→C 9.8% D57A (GAC→GCC)  carB → carbamoyl‑phosphate synthase large subunit
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,570,252 T→G 9.6% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,570,286 T→G 5.8% V234G (GTG→GGG)  yabI → DedA family protein
RA NZ_CP022686 3,625,683 T→G 13.9% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,652,079 T→G 6.3% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,688,214 T→G 6.4% N631T (AAC→ACC)  glnD ← bifunctional uridylyltransferase/uridylyl‑removing protein GlnD
RA NZ_CP022686 3,703,648 A→C 8.8% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 24.8% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,370 G→T 9.0% G226C (GGC→TGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 14.0% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,509 A→T 8.4% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 6.5% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 6.4% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 15.3% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,797,493 G→T 13.4% G134C (GGT→TGT)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,080 T→G 5.4% R329R (CGT→CGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,979 A→C 8.6% pseudogene (1255/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 10.5% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,909,314 G→T 6.2% V306F (GTC→TTC)  pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA NZ_CP022686 3,935,324 T→G 11.4% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 8.8% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,656 A→C 10.3% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,408 T→G 8.2% V105G (GTT→GGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 3,981,933 T→G 14.7% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 12.5% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,951 C→A 17.7% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,133,839 T→G 15.5% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,188,944 A→C 12.1% V321G (GTA→GGA)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA NZ_CP022686 4,214,103 T→A 9.4% intergenic (‑3/+35) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,280,095 T→G 9.2% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,287,456 T→G 9.1% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,287,463 T→A 10.2% noncoding (54/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,331,940 A→C 24.5% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,419,926 C→G 100% intergenic (‑347/+147) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
RA NZ_CP022686 4,440,314 T→G 10.3% Y791D (TAT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,324 C→A 14.8% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 15.5% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,313 G→T 7.2% A106A (GCG→GCT appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,562,600 T→G 11.5% Q123P (CAG→CCG)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,571,417 T→G 9.7% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,587,091 T→G 13.1% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 8.4% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,186 G→T 7.1% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,655,834 A→C 5.7% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,712,763 A→C 19.0% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 15.6% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,886 G→T 8.7% intergenic (‑66/‑195) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 11.1% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 22.7% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,787,799 C→A 6.8% intergenic (‑52/‑44) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,787,836 A→C 7.7% intergenic (‑89/‑7) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,814,990 T→G 11.6% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,007 T→G 8.9% P76P (CCT→CCG CIW80_RS24295 → phage tail protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 10.6% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,919,017 T→G 7.7% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,997,085 A→C 7.6% N158T (AAC→ACC)  ycjT → kojibiose phosphorylase
RA NZ_CP022686 4,997,088 A→C 8.6% H159P (CAC→CCC)  ycjT → kojibiose phosphorylase
RA NZ_CP022686 5,023,952 G→T 5.8% intergenic (+218/‑260) zntB → / → dbpA zinc transporter ZntB/ATP‑dependent RNA helicase DbpA
RA NZ_CP022686 5,029,533 A→C 5.9% intergenic (‑124/+17) uspF ← / ← ompN universal stress protein UspF/porin OmpN
RA NZ_CP022686 5,042,250 A→T 7.0% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 10.3% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→C 9.1% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 2,896 T→G 11.0% intergenic (‑475/+233) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,898 Δ1 bp 5.3% intergenic (‑477/+231) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,899 Δ1 bp 92.3% intergenic (‑478/+230) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,914 T→G 7.6% intergenic (‑493/+215) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,945 G→T 7.8% intergenic (‑524/+184) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,946 G→T 12.8% intergenic (‑525/+183) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,954 G→T 8.4% intergenic (‑533/+175) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,964 G→T 10.5% intergenic (‑543/+165) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 336–2618 3074–2957 340–2739 10886 [10852] [10842] 11021 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =3 (0.010)227 (1.110) 91/248 NT 98.7% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.010)179 (0.880) 71/248 NT 98.4% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase