breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 15.4% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 166,881 G→T 10.0% E53* (GAA→TAA)  rstB → two‑component system sensor histidine kinase RstB
RA NZ_CP022686 197,828 C→A 9.9% F461L (TTC→TTA dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 235,011 A→G 21.9% T9A (ACC→GCC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 340,516 A→C 10.6% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,090 T→A 9.3% F601L (TTT→TTA yeaG → protein kinase YeaG
RA NZ_CP022686 350,056 A→C 14.1% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 13.3% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 556,940 A→T 13.2% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 582,870 C→A 10.6% W401C (TGG→TGT ybtS ← yersiniabactin biosynthesis salicylate synthase Irp9/YbtS
RA NZ_CP022686 585,422 A→C 14.6% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 596,637 T→G 6.8% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,764 G→C 9.1% V435V (GTC→GTG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 15.5% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 16.7% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,634 C→A 6.9% intergenic (‑207/+16) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 881,733 A→C 15.6% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 13.8% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,051 G→T 11.3% intergenic (+120/‑137) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 16.5% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 18.8% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,525 A→C 7.0% S38A (TCT→GCT)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 18.1% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,431 T→G 21.5% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 16.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,065,978 A→C 13.6% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 12.2% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,172,467 C→A 11.9% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,174,279 A→T 10.1% intergenic (+8/‑36) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 20.4% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,209,917 G→T 13.2% P393Q (CCA→CAA)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,462 A→C 29.3% L426R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,213,479 T→G 12.7% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,213,483 T→G 19.5% N419T (AAC→ACC)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,237,918 C→A 12.5% C303F (TGC→TTC)  tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,249,393 C→A 6.1% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,269,551 T→G 10.8% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,319,599 T→G 9.0% T329P (ACC→CCC)  glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,360,724 G→T 8.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,559 T→G 14.3% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,441,726 A→C 7.3% L612V (TTA→GTA)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,447,152 G→T 10.1% R51S (CGC→AGC)  hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,479,607 A→C 10.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,355 T→A 12.8% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 20.0% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,365 A→C 31.7% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,110 G→C 18.6% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 10.9% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 13.5% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,604,828 A→C 8.7% L390R (CTG→CGG)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,615,220 A→C 7.3% R58R (CGT→CGG kduD ← 2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
RA NZ_CP022686 1,632,501 T→G 9.4% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,674,224 T→G 11.6% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.1% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.0% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 9.9% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,167 A→C 10.7% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,832,023 T→G 14.6% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,860,122 C→G 8.1% V101V (GTG→GTC CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,892,153 A→C 9.3% intergenic (+16/‑307) CIW80_RS09425 → / → CIW80_RS09430 FCD domain‑containing protein/mannitol dehydrogenase family protein
RA NZ_CP022686 1,954,923 T→G 19.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,049,089 T→G 20.6% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 14.4% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 10.0% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 9.8% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,130,829 A→C 16.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,105 C→G 10.3% L123V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,114 C→G 13.3% L126V (CTG→GTG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,673 C→A 12.3% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 7.9% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 7.9% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,205 G→C 5.6% A41G (GCT→GGT)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,272,797 G→T 8.6% intergenic (+39/‑58) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,310,429 T→G 8.6% N248T (AAC→ACC)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 19.3% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 13.7% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,362,949 T→G 6.3% L150R (CTG→CGG)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,470,422 T→G 8.0% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,918 T→A 5.9% S776S (TCT→TCA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,721 A→C 14.8% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,624,291 G→T 10.2% S140Y (TCC→TAC)  yidK ← solute:sodium symporter family transporter
RA NZ_CP022686 2,717,385 T→G 9.0% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,754,975 C→A 7.9% E5* (GAA→TAA)  cyaY ← iron donor protein CyaY
RA NZ_CP022686 2,758,109 A→C 8.8% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,801,884 T→G 5.0% T10P (ACC→CCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,868,740 T→G 23.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 13.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.3% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,920,417 G→C 12.1% A65P (GCA→CCA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,989,627 T→G 12.3% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,994,693 T→G 8.6% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,020,229 T→G 12.7% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,090,101 A→C 9.8% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,940 T→G 8.9% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 14.5% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,059 T→G 10.8% L93R (CTG→CGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,317,673 A→T 10.4% intergenic (+91/+28) lptG → / ← yjgR LPS export ABC transporter permease LptG/DUF853 domain‑containing protein
RA NZ_CP022686 3,334,291 C→A 9.5% G250C (GGT→TGT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,387,644 A→C 10.1% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,832 T→A 10.6% F274Y (TTC→TAC)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,459,370 C→A 14.3% S62Y (TCT→TAT)  bglJ → DNA‑binding transcriptional activator BglJ
RA NZ_CP022686 3,461,224 T→G 12.9% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 18.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,504,054 T→G 9.0% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,514,511 A→C 5.1% S190R (AGC→CGC)  CIW80_RS17610 → hypothetical protein
RA NZ_CP022686 3,538,827 A→C 6.5% L123V (TTA→GTA)  caiT ← L‑carnitine/gamma‑butyrobetaine antiporter
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,634,614 A→C 7.5% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 12.4% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,713,182 A→C 12.1% E39D (GAA→GAC yaeR → VOC family protein
RA NZ_CP022686 3,770,508 T→G 8.1% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,798,092 C→A 10.9% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,801,492 T→G 6.9% pseudogene (1768/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 12.9% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,516 A→C 6.3% intergenic (‑122/+62) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,925,283 G→C 7.2% S154T (AGC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 11.4% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,376 T→G 10.9% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,940,707 A→C 11.2% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 4,014,242 A→T 11.3% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 19.9% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,214,235 C→A 8.0% intergenic (‑23/+26) CIW80_RS21085 ← / ← CIW80_RS21090 tRNA‑Gln/tRNA‑Met
RA NZ_CP022686 4,286,455 T→G 9.2% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,331,940 A→C 15.2% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,412,269 A→C 10.6% F246V (TTT→GTT)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,419,920 A→G 100% intergenic (‑341/+153) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
RA NZ_CP022686 4,419,926 C→G 100% intergenic (‑347/+147) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
RA NZ_CP022686 4,440,329 T→A 12.5% Y796N (TAT→AAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,445,534 T→G 10.6% intergenic (+40/‑199) serS → / → dmsA serine‑‑tRNA ligase/dimethylsulfoxide reductase subunit A
RA NZ_CP022686 4,587,091 T→G 14.2% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,589,392 T→G 7.3% K238N (AAA→AAC CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 12.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,217 C→A 7.9% T288N (ACC→AAC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,764,880 T→G 9.4% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,883 A→T 10.8% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 11.0% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,787,836 A→C 10.6% intergenic (‑89/‑7) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,839,668 T→G 12.1% V150V (GTA→GTC minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,910,213 A→C 7.8% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,937,070 A→C 11.5% G172G (GGT→GGG trpA ← tryptophan synthase subunit alpha
RA NZ_CP022686 4,951,538 C→A 26.9% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 5,030,756 G→A 17.4% intergenic (‑73/+293) ompN ← / ← nifJ porin OmpN/pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA NZ_CP022686 5,042,250 A→T 6.7% V627V (GTA→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 13.3% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 8.9% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 1,534 G→A 14.8% W12* (TGG→TGA Aldafermin (NGM282) →
RA pHH‑29_RBS 1,674:1 +A 79.8% coding (176/570 nt) Aldafermin (NGM282) →
RA pHH‑29_RBS 2,892 G→T 14.5% intergenic (‑471/+237) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,898 Δ1 bp 6.6% intergenic (‑477/+231) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,899 Δ1 bp 100% intergenic (‑478/+230) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,934 C→A 9.1% intergenic (‑513/+195) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,946 G→T 14.7% intergenic (‑525/+183) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,953 G→T 12.4% intergenic (‑532/+176) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 188–2618 3085–2957 340–2898 12432 [12083] [12067] 12229 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =2 (0.010)165 (0.910) 58/250 NT 98.8% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624682 (0.010)160 (0.890) 55/250 NT 98.8% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =29 (0.160)175 (1.110) 52/218 NT 88.1% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 22 (0.140)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 389968129 (0.160)149 (0.950) 61/218 NT 86.3% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 22 (0.140)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA