breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 314,813 T→G 8.9% V204G (GTC→GGC)  ynjC → ABC transporter permease
RA NZ_CP022686 340,453 A→C 5.1% L52V (TTG→GTG)  ydjK ← MFS transporter
RA NZ_CP022686 349,997 C→A 12.1% A221A (GCC→GCA yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,279 C→A 19.8% W17C (TGG→TGT yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 20.2% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 531,408 A→C 13.4% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 597,830 A→C 12.8% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 16.9% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 12.5% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 722,957 A→C 5.9% N350T (AAC→ACC)  CIW80_RS03730 → patatin‑like phospholipase family protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 777,365 A→C 12.7% L216R (CTG→CGG)  gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 24.9% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 14.9% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 20.0% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 859,943 T→G 13.0% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 859,999 T→G 7.9% A620A (GCT→GCG yehM → DUF5682 family protein
RA NZ_CP022686 881,742 A→C 22.2% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 16.0% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,035 A→T 12.0% intergenic (+104/‑153) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 15.0% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,439 T→G 21.1% P942P (CCA→CCC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 11.1% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,047,883 T→G 7.4% intergenic (‑113/+51) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,065,978 A→C 9.9% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,172,467 C→A 15.9% A130E (GCA→GAA)  yfeD → putative DNA‑binding transcriptional regulator
RA NZ_CP022686 1,209,908 T→G 22.8% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,232,494 A→C 8.3% E635D (GAA→GAC acrD → multidrug efflux RND transporter permease AcrD
RA NZ_CP022686 1,239,833 G→T 13.7% intergenic (‑130/+38) ypfJ ← / ← purC neutral zinc metallopeptidase/phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA NZ_CP022686 1,360,709 T→G 10.4% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 8.1% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,559 T→G 11.6% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,071 G→T 16.8% D29Y (GAT→TAT)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,371,081 C→A 22.0% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,479,619 T→A 11.0% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,625 G→C 8.4% intergenic (‑34/+159) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,323 C→A 17.1% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 28.0% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,510,552 G→T 8.6% H493N (CAC→AAC)  relA ← GTP pyrophosphokinase
RA NZ_CP022686 1,535,339 G→C 14.5% E27Q (GAA→CAA)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,558,395 A→C 8.5% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 14.2% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,235 T→G 9.2% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,633,861 A→T 13.6% L248* (TTA→TAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 9.4% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 17.7% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 9.2% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.5% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 11.0% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,154 A→C 8.7% T45P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 15.1% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,833,799 T→G 12.2% T450P (ACG→CCG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,594 C→A 12.6% D227Y (GAT→TAT)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,911,602 T→G 10.2% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,955 T→G 18.3% Q81P (CAG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,990,332 A→C 7.0% G448G (GGT→GGG uxaA ← altronate dehydratase
RA NZ_CP022686 2,035,500 T→A 6.2% L145F (TTA→TTT rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,047,987 A→C 6.5% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 19.3% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 5.7% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,070,788 A→C 7.0% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,554 T→G 10.9% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,148,109 T→G 11.8% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 16.3% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 16.2% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 8.7% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 12.0% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 9.8% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,222,531 T→G 13.2% F417V (TTT→GTT)  yheS → ABC transporter ATP‑binding protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 18.6% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,954 T→G 5.8% F152V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,386,496 T→G 9.8% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,393,013 T→G 8.8% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 19.7% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,535 A→C 11.0% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 16.2% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 27.1% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 12.5% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,216 T→C 9.2% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,426 T→A 10.7% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,524,447 T→G 6.1% L148R (CTG→CGG)  trmL → tRNA (uridine(34)/cytosine(34)/5‑ carboxymethylaminomethyluridine(34)‑2'‑O)‑ methyltransferase TrmL
RA NZ_CP022686 2,558,864 T→G 6.6% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,572,705 A→C 10.9% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 13.1% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,733,571 T→G 11.4% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,733,577 T→G 12.7% F55V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 8.6% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 8.1% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.4% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,565 C→A 10.7% noncoding (67/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,801,817 T→G 8.6% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,868,740 T→G 22.1% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 12.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,852 T→G 9.1% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 9.1% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,989,627 T→G 10.3% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,994,693 T→G 15.3% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,136,940 T→G 13.1% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,348,342 A→T 9.0% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,605 T→G 20.7% K146N (AAA→AAC sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,832 T→A 12.6% F274Y (TTC→TAC)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,200 C→A 14.0% intergenic (‑9/+26) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 14.6% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,223 G→T 23.7% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,224 T→G 16.9% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,516,093 T→G 9.1% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,570,252 T→G 15.0% L223V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,604,564 G→T 13.4% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 11.5% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,493 G→T 9.5% I780I (ATC→ATA htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,734,367 T→G 22.6% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 16.3% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 9.0% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 7.4% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,806,256 G→T 12.3% E1583* (GAA→TAA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 12.3% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,928,345 T→G 12.3% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 13.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 11.3% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.5% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 12.6% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,628 T→A 13.1% L79* (TTA→TAA)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 8.5% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 10.3% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 14.2% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 14.2% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,133,850 T→G 10.5% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,331,882 A→T 11.7% M308K (ATG→AAG)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 13.7% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,372 T→G 13.1% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 12.4% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 13.0% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,745 G→T 10.9% intergenic (+99/‑71) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,535,349 A→C 12.6% T118T (ACA→ACC appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,587,091 T→G 8.7% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,587,120 G→T 10.2% S599R (AGC→AGA CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,579 T→G 17.4% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 10.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 8.9% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,764,877 T→G 15.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 9.3% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,787,834 G→C 8.2% intergenic (‑87/‑9) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,814,961 T→G 9.2% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 11.3% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,873 T→G 6.2% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,943,295 T→G 8.1% K5N (AAA→AAC trpE ← anthranilate synthase component I
RA NZ_CP022686 4,951,538 C→A 12.0% A44A (GCC→GCA topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,125 A→C 12.4% N150T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,043,280 G→T 6.2% A971S (GCG→TCG)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 7.1% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 7.0% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 8.6% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 10.2% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 2,837:1 +A 100% intergenic (‑416/+292) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–
RA pHH‑29_RBS 2,899 Δ1 bp 100% intergenic (‑478/+230) mok CDS ← / ← putative HTH domain protein (MarR‑like) –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 338–2618 3173 556–2836 7774 [7743] [650] NA [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2973–3097 5510–3245 149–2538 4992 [4353] [3951] 4878 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =1 (0.010)191 (1.020) 69/250 NT 99.5% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.010)171 (0.910) 52/250 NT 99.4% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase