breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 162,427 T→G 7.0% V53G (GTG→GGG)  ydgH → DUF1471 family protein YdgH
RA NZ_CP022686 177,506 T→G 7.8% Q140H (CAA→CAC uidB ← glucuronide transporter
RA NZ_CP022686 234,990 A→G 35.3% K2E (AAA→GAA)  pykF → pyruvate kinase PykF
JC NZ_CP022686 235,939 +TAA 8.5% coding (953/1413 nt) pykF → pyruvate kinase PykF
RA NZ_CP022686 238,323 A→C 22.6% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,324 G→C 18.8% V85V (GTC→GTG sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 11.4% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 340,511 G→C 5.6% G32G (GGC→GGG ydjK ← MFS transporter
RA NZ_CP022686 340,516 A→C 9.6% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,092 A→C 9.9% N602T (AAC→ACC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,106 A→C 12.9% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 12.9% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 14.0% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 418,374 T→G 11.5% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 481,005 A→C 14.0% L620R (CTG→CGG)  flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 496,134 G→C 15.0% L383V (CTT→GTT)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.7% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 23.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 14.2% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 692,994 G→T 10.6% D112Y (GAT→TAT)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,391 C→A 13.2% intergenic (+151/‑45) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,008 A→T 10.4% I37I (ATT→ATA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 22.5% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 20.8% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 15.8% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 10.2% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 878,883 T→G 6.5% intergenic (+75/+45) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,014 C→G 15.9% intergenic (+83/‑174) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 10.0% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 954,498 A→C 8.2% F47V (TTT→GTT)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 954,516 A→C 10.5% L41V (TTG→GTG)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 1,005,082 T→A 7.4% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 11.8% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,022,752 T→G 6.3% T44P (ACG→CCG)  yfaZ ← YfaZ family outer membrane protein
RA NZ_CP022686 1,034,493 T→G 7.5% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,065,997 A→C 10.5% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,174,402 G→T 7.4% intergenic (+12/‑35) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 20.1% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 12.5% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,325,422 G→T 5.2% Q252K (CAA→AAA)  qseE ← two component system sensor histidine kinase QseE/GlrK
RA NZ_CP022686 1,353,233 A→C 19.2% V72G (GTT→GGT)  yfiF ← tRNA/rRNA methyltransferase
RA NZ_CP022686 1,360,724 G→T 14.1% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 8.0% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,742 C→A 8.9% intergenic (‑77/+247) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 6.9% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,783 G→T 8.2% intergenic (‑118/+206) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,565 A→T 9.0% I96I (ATT→ATA rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,612 C→A 10.2% D81Y (GAT→TAT)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,424,342 C→G 5.8% intergenic (‑38/+25) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,424,347 A→T 7.2% intergenic (‑43/+20) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,424,363 G→T 10.3% intergenic (‑59/+4) CIW80_RS07085 ← / ← CIW80_RS07090 tRNA‑Arg/tRNA‑Arg
RA NZ_CP022686 1,441,772 A→C 9.3% R596R (CGT→CGG hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,465,000 G→T 8.2% E173* (GAA→TAA)  pphB → protein‑serine/threonine phosphatase
RA NZ_CP022686 1,479,607 A→C 12.3% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 9.4% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,633 A→C 12.1% intergenic (‑42/+151) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,323 C→A 11.4% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 17.8% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 10.2% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 9.2% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,572,185 A→C 6.7% S143R (AGC→CGC)  tssJ → type VI secretion system lipoprotein TssJ
RA NZ_CP022686 1,597,235 T→G 8.9% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,619,230 C→A 8.1% intergenic (+19/+298) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,501 T→G 7.9% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,854 C→A 29.7% V250V (GTG→GTT yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 11.6% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 12.7% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 14.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.8% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,692,094 G→T 10.0% I174I (ATC→ATA CIW80_RS08330 ← nucleoside/nucleotide kinase family protein
RA NZ_CP022686 1,705,974 T→G 9.5% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,705,984 T→G 12.6% intergenic (‑594/‑61) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,705,997 C→A 15.0% intergenic (‑607/‑48) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,167 A→C 10.1% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,735,916 A→T 7.3% pseudogene (353/569 nt) papA ← P fimbria major subunit PapA
RA NZ_CP022686 1,758,771 T→G 5.8% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,805,056 A→C 6.3% pseudogene (337/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,911,602 T→G 6.8% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,365 A→C 7.1% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 19.2% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,144 C→G 5.8% R96P (CGC→CCC)  nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 1,965,173 A→C 8.3% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 1,975,137 A→C 8.3% intergenic (+6/‑57) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 1,975,144 C→A 5.8% intergenic (+13/‑50) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 2,016,153 T→G 6.3% noncoding (121/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,160 T→G 6.6% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 6.9% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,217 T→A 5.9% noncoding (57/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 6.5% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,032,739 G→T 5.7% W24L (TGG→TTG)  agaC → PTS galactosamine transporter subunit IIC
RA NZ_CP022686 2,035,536 A→C 7.4% G133G (GGT→GGG rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,037,521 A→C 9.1% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 21.0% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,097 A→T 13.4% R253R (CGT→CGA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,552 A→C 8.3% intergenic (+22/‑211) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,554 T→G 11.2% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 11.4% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 11.1% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 10.5% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 11.8% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,672 G→C 9.4% A167P (GCA→CCA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,152 G→T 8.2% Q96K (CAG→AAG)  smg ← DUF494 family protein Smg
RA NZ_CP022686 2,172,165 G→T 5.4% F91L (TTC→TTA smg ← DUF494 family protein Smg
RA NZ_CP022686 2,172,177 A→C 8.3% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,071 A→C 7.0% G47G (GGT→GGG rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 9.7% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,208,142 A→C 5.6% V569G (GTT→GGT)  chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,209,101 A→T 5.7% I249I (ATT→ATA chiA ← bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,206 C→A 5.3% A41S (GCT→TCT)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,226,139 A→C 7.6% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,340,264 A→C 6.6% V91G (GTC→GGC)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,356,231 T→G 8.4% D9A (GAC→GCC)  tusA ← sulfurtransferase TusA
RA NZ_CP022686 2,357,324 A→C 18.2% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,954 T→G 5.5% F152V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,383,165 G→T 5.6% intergenic (+56/+58) uspA → / ← CIW80_RS26230 universal stress protein UspA/hypothetical protein
RA NZ_CP022686 2,386,496 T→G 6.9% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,397,372 C→G 9.2% L10V (CTT→GTT)  chuT → heme ABC transporter substrate‑binding protein ChuT
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,443,289 A→C 10.3% V9V (GTT→GTG bcsQ ← cellulose biosynthesis protein BcsQ
RA NZ_CP022686 2,470,422 T→G 9.0% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,499,319 T→G 7.4% N44T (AAC→ACC)  aldB ← aldehyde dehydrogenase AldB
RA NZ_CP022686 2,516,426 T→A 11.5% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 12.6% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 13.3% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,423 A→C 17.2% V85V (GTT→GTG waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,555,399 T→A 11.1% intergenic (‑26/+40) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH
RA NZ_CP022686 2,572,705 A→C 11.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 8.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,577,168 G→T 5.7% T487N (ACC→AAC)  CIW80_RS12915 ← PRD domain‑containing protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,703,299 A→C 7.5% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,717,400 T→G 6.6% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,586 A→C 8.4% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,737,495 T→G 6.1% L109V (TTG→GTG)  wzxE → lipid III flippase WzxE
RA NZ_CP022686 2,743,563 T→G 10.1% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,747,591 T→G 5.4% N395T (AAC→ACC)  hemY ← protoheme IX biogenesis protein HemY
RA NZ_CP022686 2,758,153 A→C 9.4% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,769,166 C→A 14.6% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,186 C→A 14.3% M50I (ATG→ATT rarD ← EamA family transporter RarD
RA NZ_CP022686 2,808,127 T→G 8.4% V441G (GTC→GGC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,821,644 A→C 8.4% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,849,863 G→T 8.8% I174I (ATC→ATA CIW80_RS14255 ← DeoR/GlpR family DNA‑binding transcription regulator
RA NZ_CP022686 2,852,911 T→G 8.8% G172G (GGT→GGG CIW80_RS14270 → aldolase
RA NZ_CP022686 2,868,740 T→G 23.7% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 7.8% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,871,449 T→G 6.4% D140A (GAC→GCC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 9.3% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,915 T→G 5.3% T218P (ACC→CCC)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,937,218 C→A 9.4% E216* (GAA→TAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,937,239 T→G 9.3% T209P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,989,627 T→G 9.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 12.7% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 12.3% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,098,847 A→C 6.3% intergenic (+17/+22) CIW80_RS15415 → / ← actP LrgB family protein/cation/acetate symporter ActP
RA NZ_CP022686 3,136,940 T→G 10.4% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,948 G→T 9.3% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,151,862 T→G 6.7% F176V (TTT→GTT)  proP → glycine betaine/L‑proline transporter ProP
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 5.7% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,209,121 T→G 6.1% T236P (ACC→CCC)  rsgA ← small ribosomal subunit biogenesis GTPase RsgA
RA NZ_CP022686 3,218,260 T→G 7.3% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,252,190 T→G 13.4% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,348,371 T→G 9.7% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,104 C→A 5.9% H5N (CAT→AAT)  CIW80_RS16820 → Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.7% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,224 T→G 14.9% intergenic (‑33/+2) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 18.0% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 14.2% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,922 C→G 6.7% A123P (GCC→CCC)  yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,516,075 T→G 8.3% V213G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,516,093 T→G 6.3% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 20.3% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,079 T→G 11.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 9.1% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,657,091 C→A 9.4% E248* (GAA→TAA)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 16.8% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,665,943 A→C 8.8% K474N (AAA→AAC hrpB → ATP‑dependent helicase HrpB
RA NZ_CP022686 3,703,648 A→C 6.5% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 22.6% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 15.3% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,403 C→G 7.2% L237V (CTG→GTG)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 9.0% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,524 T→G 5.3% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 13.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,080 T→G 5.4% R329R (CGT→CGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,811,480 G→T 42.5% G122C (GGT→TGT)  CIW80_RS19090 → hypothetical protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 8.7% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,928,345 T→G 8.0% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 9.3% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,932,443 C→A 16.0% E184* (GAA→TAA)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 12.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 9.6% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 9.2% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,655 A→C 5.5% K88T (AAA→ACA) ‡ phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,977,656 A→C 8.4% K88N (AAA→AAC) ‡ phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 9.4% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,004,135 T→G 7.7% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,238 C→G 11.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 12.9% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 20.4% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,265 A→C 8.4% Q97H (CAA→CAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,951 C→A 15.5% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 20.0% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,061,217 A→C 5.4% L431R (CTG→CGG)  ybaL ← YbaL family putative K(+) efflux transporter
RA NZ_CP022686 4,213,260 T→G 8.1% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,213,266 A→T 8.7% L190F (TTA→TTT ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,214,103 T→A 9.1% intergenic (‑3/+35) CIW80_RS21080 ← / ← CIW80_RS21085 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,435 G→C 10.0% intergenic (‑8/+27) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,457 G→T 12.5% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,234,411 C→A 13.7% intergenic (+111/‑331) glnS → / → chiP glutamine‑‑tRNA ligase/chitoporin
RA NZ_CP022686 4,272,588 T→G 7.3% G139G (GGT→GGG odhB → 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase
RA NZ_CP022686 4,280,095 T→G 7.8% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,283,890 A→C 7.7% K268N (AAA→AAC tolA → cell envelope integrity protein TolA
RA NZ_CP022686 4,286,467 T→G 5.4% V42G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,287,463 T→A 6.4% noncoding (54/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,327,816 T→G 5.5% F110V (TTC→GTC)  ybhM → Bax inhibitor‑1/YccA family protein
RA NZ_CP022686 4,368,372 T→G 10.8% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,334 A→T 16.6% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,470,157 T→G 6.1% V81G (GTC→GGC)  lpxK → tetraacyldisaccharide 4'‑kinase
RA NZ_CP022686 4,485,614 T→G 9.1% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,587,091 T→G 15.2% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,587,135 T→G 7.3% K594N (AAA→AAC CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 11.9% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 6.8% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,655,834 A→C 7.1% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,678,037 C→A 13.7% intergenic (+35/‑86) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,041 A→C 17.1% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 23.4% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 17.0% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 10.4% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,883 A→T 9.6% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 7.6% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,809,441 T→G 10.3% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,809,446 C→G 15.8% A52G (GCC→GGC)  CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,990 T→G 9.2% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 14.6% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,839,701 G→T 10.6% A139A (GCC→GCA minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,850,783 C→A 9.1% M215I (ATG→ATT ycgB ← SpoVR family protein
RA NZ_CP022686 4,855,492 A→C 8.3% L146R (CTT→CGT)  cvrA ← potassium/proton antiporter
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,909,108 A→T 8.4% intergenic (‑5/+42) CIW80_RS24835 ← / ← CIW80_RS24840 RtT sRNA/tRNA‑Tyr
RA NZ_CP022686 4,916,128 A→C 8.0% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,918,846 C→A 6.9% Q387H (CAG→CAT adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,919,017 T→G 8.8% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,952,020 T→G 9.7% V205G (GTC→GGC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,001,157 G→T 15.0% D161Y (GAT→TAT)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,023,944 T→G 7.2% intergenic (+210/‑268) zntB → / → dbpA zinc transporter ZntB/ATP‑dependent RNA helicase DbpA
RA NZ_CP022686 5,042,172 T→A 9.6% S601R (AGT→AGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.1% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 13.1% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 1,534 G→A 100% W12* (TGG→TGA Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 206–2618 3084–2956 339–2879 12913 [12722] [12418] 13320 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2777–2897 5512–3245 349–2736 6343 [6102] [6273] 7047 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =2 (0.010)199 (0.840) 71/248 NT 99.0% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.000)192 (0.810) 74/248 NT 99.5% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =215 (0.910)81 (0.390) 46/216 NT 32.3% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 153 (0.740)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681206 (0.870)74 (0.360) 48/216 NT 30.8% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 153 (0.740)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA