breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NZ_CP022686 | 457,895 | Δ1 bp | pseudogene (287/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 560,880 | C→T | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | [clbP] | [clbP] | |
RA | NZ_CP022686 | 756,115 | A→G | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 794,460 | C→T | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 917,427 | +TT | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 2,214,419 | T→C | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,320,775 | C→T | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,408,862 | Δ1 bp | pseudogene (1098/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
JC | NZ_CP022686 | 2,590,564 | +TGGT | coding (110/153 nt) | CIW80_RS26455 → | hypothetical protein |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,424,795 | A→C | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
RA | NZ_CP022686 | 3,656,188 | A→G | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,792,805 | A→G | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
MC JC | NZ_CP022686 | 3,844,426 | Δ1,036 bp | coding (2‑1037/1056 nt) | dinB → | DNA polymerase IV |
MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | [umuD]–[umuC] | [umuD], [umuC] | |
RA | NZ_CP022686 | 4,870,914 | Δ1 bp | intergenic (‑110/‑118) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | [IL2wt no signal] | [IL2wt no signal] |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 206–2618 | 3084–2957 | 340–2879 | 8181 [8084] | [7985] 8791 | JNG15_RS00005–mobA | JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4750–4810 | 5478–5075 | 266–729 | 3517 [3495] | [1] NA | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | 262466 = | 6 (0.030) | 163 (0.940) | 63/270 | 0.4 | 96.5% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
* | ? | NZ_CP022686 | = 262468 | 3 (0.020) | 153 (0.880) | 62/270 | 0.5 | 98.1% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase |