breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA NZ_CP022686 457,895 Δ1 bp pseudogene (287/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
MC JC NZ_CP022686 628,478 Δ1,286 bp [clbP] [clbP]
RA NZ_CP022686 756,115 A→G G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 917,427 +TT intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 2,214,419 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,408,862 Δ1 bp pseudogene (1098/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
JC NZ_CP022686 2,590,564 +TGGT coding (110/153 nt) CIW80_RS26455 → hypothetical protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,424,795 A→C intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
MC JC NZ_CP022686 3,559,837 Δ2,343 bp coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,656,188 A→G S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,792,805 A→G Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp coding (2‑1037/1056 nt) dinB → DNA polymerase IV
MC JC NZ_CP022686 4,844,868 Δ1,662 bp [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,914 Δ1 bp intergenic (‑110/‑118) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 206–2618 3084–2957 340–2879 8181 [8084] [7985] 8791 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4750–4810 5478–5075 266–729 3517 [3495] [1] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =6 (0.030)163 (0.940) 63/270 0.4 96.5% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.020)153 (0.880) 62/270 0.5 98.1% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase