breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 190,715 T→G 10.7% R42R (CGT→CGG rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 196,816 T→G 11.8% V124G (GTC→GGC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 238,317 G→T 22.3% L88I (CTT→ATT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,323 A→C 22.0% F86V (TTT→GTT)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 263,831 A→C 14.9% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 311,453 G→T 13.3% D116Y (GAT→TAT)  ydjY → lipoprotein YdjY
RA NZ_CP022686 340,516 A→C 13.1% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 360,227 T→G 23.8% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,135 A→C 13.8% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 556,940 A→T 22.2% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 20.3% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 18.3% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.6% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 13.3% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,014 A→T 11.9% V35V (GTT→GTA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,757 T→G 15.5% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 22.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,733 A→C 18.1% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,742 A→C 22.0% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 20.1% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,029 C→A 14.4% intergenic (+98/‑159) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,046 G→T 14.6% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,174,272 A→C 15.2% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 17.5% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,462 A→C 30.4% L426R (CTG→CGG)  eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,249,393 C→A 8.2% intergenic (‑40/+46) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase
RA NZ_CP022686 1,280,117 T→G 6.4% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,358,687 T→G 7.4% L348V (TTA→GTA)  pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA NZ_CP022686 1,360,724 G→T 8.2% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 7.5% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,086 G→T 18.2% E34* (GAA→TAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,479,607 A→C 11.6% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,634 G→C 11.8% intergenic (‑43/+150) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,324 C→A 17.4% P254H (CCT→CAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 9.7% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 9.1% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,846 T→G 29.1% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 20.8% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 8.0% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,911,602 T→G 9.5% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,923 T→G 15.5% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,975,137 A→C 8.6% intergenic (+6/‑57) ebgC → / → ygjI beta‑galactosidase subunit beta/amino acid permease
RA NZ_CP022686 2,016,170 A→C 6.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,029,857 C→A 8.2% intergenic (+246/‑103) nagA → / → agaS N‑acetylglucosamine‑6‑phosphate deacetylase/AgaS family sugar isomerase
RA NZ_CP022686 2,049,089 T→G 19.2% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 8.7% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,076,554 T→G 14.5% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 16.1% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,461 A→T 7.1% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 10.4% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,109 T→G 14.0% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 12.0% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,188,239 G→T 8.1% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,305,971 G→T 9.8% S222* (TCA→TAA)  CIW80_RS11625 ← fimbrial biogenesis usher protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,340,264 A→C 5.6% V91G (GTC→GGC)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,357,291 A→C 9.8% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,322 T→A 12.9% S36R (AGT→AGA dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,972 T→G 5.1% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,393,013 T→G 7.3% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,394,287 T→G 17.9% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 24.6% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,516,216 T→C 8.1% S542S (TCT→TCC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,400 A→C 16.5% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,558,864 T→G 6.8% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,559,398 T→G 6.9% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,705 A→C 13.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,715 G→T 14.9% S188Y (TCC→TAC)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 14.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 11.5% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,703,299 A→C 8.1% intergenic (‑203/‑277) yieP ← / → CIW80_RS13540 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA NZ_CP022686 2,801,864 T→G 5.2% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,821,644 A→C 8.7% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,868,740 T→G 23.3% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 13.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,874,109 A→C 10.1% T74P (ACG→CCG)  CIW80_RS14390 → carbohydrate porin
RA NZ_CP022686 2,904,852 T→G 8.7% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,385 C→A 5.5% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,961,313 G→C 6.5% intergenic (+91/‑282) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,989,627 T→G 7.2% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,994,693 T→G 15.9% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,020,229 T→G 11.1% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,184,651 A→C 11.9% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,348,342 A→T 11.8% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 10.3% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,154 A→C 8.8% K21N (AAA→AAC CIW80_RS16820 → Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 15.0% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,127 A→C 10.2% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 17.4% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 14.9% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,516,065 C→A 15.7% L210M (CTG→ATG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,516,073 C→G 6.3% G212G (GGC→GGG nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,606,042 G→C 7.2% E252Q (GAA→CAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,652,079 T→G 11.6% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,231 G→A 12.7% R105C (CGT→TGT)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,661,518 C→A 11.6% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,734,367 T→G 13.9% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,372 C→G 12.1% G226G (GGC→GGG yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,776,894 G→T 7.4% L132F (TTG→TTT CIW80_RS18945 → inovirus‑type Gp2 protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,852,459 T→G 7.2% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 15.8% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,940,724 A→C 14.1% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,631 A→C 15.3% H80P (CAC→CCC)  phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,381 T→G 7.0% L96R (CTT→CGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 4,014,238 C→G 18.3% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 22.5% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,196,271 A→C 12.4% L17R (CTG→CGG)  holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,287,503 T→G 9.1% noncoding (16/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,356,139 A→C 7.1% V129G (GTT→GGT)  rhtA ← threonine/homoserine exporter RhtA
RA NZ_CP022686 4,361,126 C→A 9.4% S351* (TCG→TAG)  ybiR → anion transporter
RA NZ_CP022686 4,368,341 T→G 18.6% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,440,324 C→A 14.1% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 12.2% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,535,285 A→C 10.5% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,587,091 T→G 13.4% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 9.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,047 A→C 26.5% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 21.5% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,713,008 A→C 5.1% intergenic (‑90/+44) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,764,886 G→T 12.4% intergenic (‑66/‑195) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 13.0% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,961 T→G 8.1% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,951,548 G→C 15.2% A48P (GCC→CCC)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,042,304 T→C 9.5% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 5.6% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 6.2% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 5.8% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 13.9% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,507 A→C 10.3% intergenic (‑1711/‑24) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,524 G→T 7.4% intergenic (‑1728/‑7) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 5.6% E52* (GAA→TAA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,784 T→G 57.2% L59* (TTA→TGA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,806:1 +A 100% intergenic (‑171/+403) annotated as hok_sok locus ← / ← putative HTH domain protein –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 4,868 Δ1 bp 100% intergenic (‑233/+341) annotated as hok_sok locus ← / ← putative HTH domain protein –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 623–2618 3173 556–2551 8132 [7815] [694] NA [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4942–5066 5478–5214 149–537 4886 [4223] [1] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =3 (0.020)152 (0.860) 50/250 NT 98.1% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.020)155 (0.880) 48/250 NT 98.1% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 4514374 =142 (0.810)5 (0.000)
+AGCT
5/242 NT 6.3% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 11 (0.000)coding (1/399 nt) IL2wt no signal