breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 8.8% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 126,825 G→T 5.9% G64C (GGT→TGT)  ydeE → efflux MFS transporter YdeE
RA NZ_CP022686 192,617 A→C 8.9% E676D (GAA→GAC rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 340,516 A→C 10.6% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 350,056 A→C 15.4% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 15.2% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,290 T→G 9.9% T14P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,563 T→A 8.5% V271V (GTT→GTA sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 372,574 A→C 14.8% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,274 A→C 12.5% K136N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 402,866 G→T 7.3% pseudogene (793/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 402,874 A→C 6.3% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 418,374 T→G 8.3% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 442,342 A→C 7.2% S80A (TCG→GCG)  CIW80_RS02340 ← phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 474,679 C→A 7.2% D292Y (GAT→TAT)  torZ ← trimethylamine N‑oxide reductase TorZ
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,420 A→C 19.6% W586G (TGG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 601,295 T→G 14.6% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.6% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,337 T→G 11.2% V1621G (GTT→GGT)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 631,182 A→C 5.9% A351A (GCT→GCG clbO ← colibactin polyketide synthase ClbO
RA NZ_CP022686 651,848 A→C 9.9% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 687,661 A→C 7.4% G107G (GGT→GGG CIW80_RS03525 ← ParB N‑terminal domain‑containing protein
RA NZ_CP022686 694,406 G→T 11.6% intergenic (+166/‑30) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 694,438 G→T 12.8% M1M (ATG→ATT) † CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 694,457 G→T 9.9% E8* (GAA→TAA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 14.4% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 16.2% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 10.7% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 11.3% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.4% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 11.2% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,513 C→A 10.2% intergenic (‑102/+125) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 21.5% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 881,733 A→C 12.8% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,049 A→T 9.2% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 11.7% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 13.1% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,000,439 T→G 22.3% P942P (CCA→CCC yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,000,443 T→G 15.5% D941A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 15.9% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,100,907 T→G 18.8% E199D (GAA→GAC aroC ← chorismate synthase
RA NZ_CP022686 1,100,944 T→G 12.5% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,305 A→T 7.6% intergenic (+34/‑10) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,213,479 T→G 11.4% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,280,117 T→G 11.4% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,280,122 T→G 6.1% N6T (AAC→ACC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,709 T→G 8.9% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 9.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,392,487 A→C 11.3% T43P (ACG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase
RA NZ_CP022686 1,447,199 A→C 7.0% V35G (GTA→GGA)  hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,447,975 C→A 7.1% Q165H (CAG→CAT hycG ← formate hydrogenlyase subunit HycG
RA NZ_CP022686 1,479,607 A→C 15.7% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,250 T→G 12.8% V229V (GTT→GTG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,257 A→T 8.1% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,339 C→A 17.8% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 8.6% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,307 A→C 9.4% D16A (GAC→GCC)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,535,366 G→C 21.4% A36P (GCC→CCC)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,564,543 A→C 7.1% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,569 A→C 9.8% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,480 T→G 8.3% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,640,925 A→C 10.8% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,231 A→T 11.9% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 14.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.9% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 15.6% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,007 G→C 6.9% intergenic (‑617/‑38) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,023 T→A 6.1% intergenic (‑633/‑22) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 11.1% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,041 A→T 7.7% intergenic (‑651/‑4) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,767 T→G 5.6% G241G (GGT→GGG metK → methionine adenosyltransferase
RA NZ_CP022686 1,785,695 T→G 8.7% pseudogene (16/186 nt) CIW80_RS08875 ← zinc‑ribbon domain‑containing protein
RA NZ_CP022686 1,828,113 A→C 9.0% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,829,636 T→G 5.8% T87P (ACC→CCC)  gspH ← type II secretion system minor pseudopilin GspH
RA NZ_CP022686 1,860,077 G→T 13.1% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,925,332 A→C 10.7% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,365 A→C 8.9% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,965,173 A→C 9.2% R86R (CGT→CGG nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,170 A→C 10.5% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,175 T→G 7.1% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,224 G→C 6.2% noncoding (50/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 6.8% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 15.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 7.4% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,097 A→T 12.1% R253R (CGT→CGA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,070,788 A→C 9.4% intergenic (‑80/‑169) greA ← / → dacB transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA NZ_CP022686 2,076,554 T→G 12.5% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 9.1% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 8.0% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,234 T→A 7.4% Y210F (TAT→TTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 12.2% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 9.4% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 10.9% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,118 C→G 10.8% A127G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 7.7% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 7.7% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,888 C→A 7.7% E32* (GAA→TAA)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,179,916 G→T 6.8% R22R (CGC→CGA rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 11.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,284,668 A→C 6.7% R207R (CGT→CGG malQ ← 4‑alpha‑glucanotransferase
RA NZ_CP022686 2,310,429 T→G 6.2% N248T (AAC→ACC)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 8.0% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,322 T→A 11.8% S36R (AGT→AGA dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 17.5% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,393,013 T→G 6.4% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,398,885 A→C 9.2% S203R (AGC→CGC)  hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,398,896 A→C 10.6% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,421,692 G→C 7.5% D327H (GAC→CAC)  yhjD → inner membrane protein YhjD
RA NZ_CP022686 2,448,912 G→T 9.8% A180S (GCG→TCG)  yhjV → aromatic amino acid transport family protein
RA NZ_CP022686 2,460,526 A→C 9.1% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,535 A→C 11.0% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 10.0% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,216 T→G 8.4% S542S (TCT→TCG upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,516,577 A→G 8.0% T663A (ACA→GCA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,381 A→C 11.5% A99A (GCT→GCG waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 11.5% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,423 A→C 13.1% V85V (GTT→GTG waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,305 T→G 5.4% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
RA NZ_CP022686 2,572,705 A→C 10.8% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 9.5% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,717,400 T→G 5.8% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,733,571 T→G 10.5% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,733,577 T→G 9.8% F55V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,578 C→A 10.5% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 9.6% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.0% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,769,196 A→C 17.2% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,810,051 G→C 6.5% E246Q (GAA→CAA)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,821,644 A→C 8.1% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,868,740 T→G 22.0% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.4% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,856 T→G 6.1% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,385 C→A 5.2% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,920,411 G→T 8.3% E63* (GAA→TAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,933,245 A→C 10.2% S26R (AGC→CGC)  frwB → PTS fructose‑like transporter subunit IIB
RA NZ_CP022686 2,961,318 A→C 6.3% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 2,989,627 T→G 9.4% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,994,693 T→G 8.8% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,020,229 T→G 14.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,080,461 T→G 7.5% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,090,101 A→C 8.3% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,940 T→G 12.5% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,225,449 T→G 5.7% V162G (GTC→GGC)  rnr → ribonuclease R
RA NZ_CP022686 3,252,190 T→G 14.5% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,252,198 C→A 16.9% E219* (GAA→TAA)  CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,265,084 T→G 17.4% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,348,371 T→G 7.4% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,154 A→C 7.8% K21N (AAA→AAC CIW80_RS16820 → Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,390,629 C→A 10.0% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,221 T→G 14.3% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 23.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 8.0% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,496,452 A→C 12.4% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA NZ_CP022686 3,516,093 T→G 6.9% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,597,494 T→G 9.3% F281V (TTT→GTT)  murG → undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase
RA NZ_CP022686 3,634,591 G→T 10.6% D260Y (GAT→TAT)  acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 13.5% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,703,648 A→C 7.2% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 15.1% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 11.5% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 7.3% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,535 C→G 6.6% intergenic (‑271/+192) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 15.9% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,080 T→G 8.4% R329R (CGT→CGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,083 C→A 8.7% I330I (ATC→ATA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,092 C→A 10.3% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 8.8% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,262 G→T 8.5% D1585Y (GAT→TAT)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,806,265 T→A 10.3% Y1586N (TAC→AAC) ‡ cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,806,266 A→C 10.5% Y1586S (TAC→TCC) ‡ cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,852,459 T→G 5.0% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 8.5% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,457 A→C 9.3% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,925,283 G→C 8.6% S154T (AGC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,289 A→C 14.1% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,309 G→T 13.4% E163* (GAA→TAA)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,932,443 C→A 15.5% E184* (GAA→TAA)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 13.1% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,949,969 T→G 9.6% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 13.4% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 3,984,841 T→G 7.8% R67R (CGT→CGG queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA
RA NZ_CP022686 4,000,557 G→T 8.9% A51A (GCC→GCA xseB ← exodeoxyribonuclease VII small subunit
RA NZ_CP022686 4,014,238 C→G 16.5% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 12.4% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,292 A→C 18.7% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,067,840 A→C 5.4% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,133,839 T→G 10.1% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,234,396 C→A 8.0% intergenic (+96/‑346) glnS → / → chiP glutamine‑‑tRNA ligase/chitoporin
RA NZ_CP022686 4,287,491 T→G 7.8% noncoding (4/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,287,499 T→G 9.9% noncoding (12/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,290,599 T→G 7.0% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,940 A→C 20.3% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 14.9% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,427,643 A→C 10.0% T4P (ACG→CCG)  clpS → ATP‑dependent Clp protease adapter ClpS
RA NZ_CP022686 4,440,296 G→C 7.5% V785L (GTT→CTT) ‡ ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,297 T→A 7.6% V785D (GTT→GAT) ‡ ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,324 C→A 13.9% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→C 16.1% Q797H (CAA→CAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,654 T→G 6.8% V152G (GTT→GGT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,562,550 T→G 17.5% N140H (AAC→CAC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,616,703 T→G 12.0% intergenic (+185/+348) CIW80_RS23075 → / ← CIW80_RS26560 hypothetical protein/ATP‑binding protein
RA NZ_CP022686 4,632,182 T→G 9.3% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 7.5% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,661,469 C→A 11.0% pseudogene (569/655 nt) CIW80_RS26570 → hypothetical protein
RA NZ_CP022686 4,678,041 A→C 14.6% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,764 G→C 18.2% A52G (GCT→GGT)  yceB ← lipoprotein
RA NZ_CP022686 4,764,882 T→G 7.3% intergenic (‑62/‑199) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 13.6% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,789,702 G→T 5.9% T67T (ACC→ACA CIW80_RS24095 ← DUF1828 domain‑containing protein
RA NZ_CP022686 4,814,961 T→G 9.4% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,814,990 T→G 8.2% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 9.8% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,815,886 T→G 7.9% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,170 T→G 11.4% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,837 T→G 7.5% K390N (AAA→AAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,918,873 T→G 9.7% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,951,545 A→C 11.8% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 11.3% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,043,558 T→C 5.7% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 13.7% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,951 T→C 9.5% G1194G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 11.4% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 667–2618 3050–2957 340–2384 5954 [5438] [5470] 5732 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4740–4809 5478–5214 406–739 4832 [4568] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =1 (0.000)221 (0.970) 86/250 NT 99.5% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.000)201 (0.880) 78/250 NT 99.5% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 = 38444253 (0.010)195 (0.860) 87/248 NT 98.5% coding (1/1056 nt) dinB DNA polymerase IV
?NZ_CP022686 3845462 = 3 (0.010)coding (1038/1056 nt) dinB DNA polymerase IV
* ? NZ_CP022686 4514374 =214 (0.940)6 (0.000)
+AGCT
4/242 NT 5.3% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 8 (0.000)coding (1/399 nt) IL2wt no signal