breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_CP022686 | 119,184 | T→G | 8.8% | F47V (TTT→GTT) | marB → | multiple antibiotic resistance protein MarB |
RA | NZ_CP022686 | 126,825 | G→T | 5.9% | G64C (GGT→TGT) | ydeE → | efflux MFS transporter YdeE |
RA | NZ_CP022686 | 192,617 | A→C | 8.9% | E676D (GAA→GAC) | rsxC → | electron transport complex subunit RsxC |
RA | NZ_CP022686 | 340,516 | A→C | 10.6% | F31V (TTT→GTT) | ydjK ← | MFS transporter |
RA | NZ_CP022686 | 350,056 | A→C | 15.4% | K241T (AAA→ACA) | yeaH → | YeaH/YhbH family protein |
RA | NZ_CP022686 | 360,227 | T→G | 15.2% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 360,290 | T→G | 9.9% | T14P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 372,563 | T→A | 8.5% | V271V (GTT→GTA) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 372,574 | A→C | 14.8% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 377,274 | A→C | 12.5% | K136N (AAA→AAC) | manX → | PTS mannose transporter subunit IIAB |
RA | NZ_CP022686 | 402,866 | G→T | 7.3% | pseudogene (793/2061 nt) | ptrB ← | oligopeptidase B |
RA | NZ_CP022686 | 402,874 | A→C | 6.3% | pseudogene (785/2061 nt) | ptrB ← | oligopeptidase B |
RA | NZ_CP022686 | 418,374 | T→G | 8.3% | F48V (TTT→GTT) | znuB → | zinc ABC transporter permease subunit ZnuB |
RA | NZ_CP022686 | 442,342 | A→C | 7.2% | S80A (TCG→GCG) | CIW80_RS02340 ← | phage tail protein |
RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 474,679 | C→A | 7.2% | D292Y (GAT→TAT) | torZ ← | trimethylamine N‑oxide reductase TorZ |
RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
RA | NZ_CP022686 | 585,420 | A→C | 19.6% | W586G (TGG→GGG) | ybtQ ← | yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ |
RA | NZ_CP022686 | 601,295 | T→G | 14.6% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,302 | T→G | 22.6% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,337 | T→G | 11.2% | V1621G (GTT→GGT) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
RA | NZ_CP022686 | 631,182 | A→C | 5.9% | A351A (GCT→GCG) | clbO ← | colibactin polyketide synthase ClbO |
RA | NZ_CP022686 | 651,848 | A→C | 9.9% | R238R (CGT→CGG) | clbJ ← | colibactin non‑ribosomal peptide synthetase ClbJ |
RA | NZ_CP022686 | 687,661 | A→C | 7.4% | G107G (GGT→GGG) | CIW80_RS03525 ← | ParB N‑terminal domain‑containing protein |
RA | NZ_CP022686 | 694,406 | G→T | 11.6% | intergenic (+166/‑30) | CIW80_RS03555 → / → CIW80_RS03560 | transposase/PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 694,438 | G→T | 12.8% | M1M (ATG→ATT) † | CIW80_RS03560 → | PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 694,457 | G→T | 9.9% | E8* (GAA→TAA) | CIW80_RS03560 → | PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 764,997 | T→G | 14.4% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 829,441 | C→A | 16.2% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,448 | T→A | 10.7% | intergenic (‑37/+190) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,452 | G→T | 11.3% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,467 | C→A | 18.4% | intergenic (‑56/+171) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,472 | G→T | 11.2% | intergenic (‑61/+166) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,513 | C→A | 10.2% | intergenic (‑102/+125) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 837,555 | T→G | 21.5% | intergenic (‑128/+95) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
RA | NZ_CP022686 | 881,733 | A→C | 12.8% | intergenic (+248/+139) | yohP → / ← dusC | protein YohP/tRNA dihydrouridine(16) synthase DusC |
RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 948,049 | A→T | 9.2% | intergenic (+118/‑139) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,080 | G→T | 11.7% | intergenic (+149/‑108) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,090 | C→A | 13.1% | intergenic (+159/‑98) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 1,000,439 | T→G | 22.3% | P942P (CCA→CCC) | yfaL ← | AIDA‑I family autotransporter adhesin YfaL/EhaC |
RA | NZ_CP022686 | 1,000,443 | T→G | 15.5% | D941A (GAC→GCC) | yfaL ← | AIDA‑I family autotransporter adhesin YfaL/EhaC |
RA | NZ_CP022686 | 1,005,085 | C→A | 15.9% | T380T (ACC→ACA) | nrdA → | ribonucleoside‑diphosphate reductase subunit alpha |
RA | NZ_CP022686 | 1,100,907 | T→G | 18.8% | E199D (GAA→GAC) | aroC ← | chorismate synthase |
RA | NZ_CP022686 | 1,100,944 | T→G | 12.5% | D187A (GAC→GCC) | aroC ← | chorismate synthase |
RA | NZ_CP022686 | 1,174,305 | A→T | 7.6% | intergenic (+34/‑10) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
RA | NZ_CP022686 | 1,213,479 | T→G | 11.4% | G420G (GGA→GGC) | eutE ← | aldehyde dehydrogenase EutE |
RA | NZ_CP022686 | 1,280,117 | T→G | 11.4% | N8H (AAC→CAC) | yfgM ← | ancillary SecYEG translocon subunit YfgM |
RA | NZ_CP022686 | 1,280,122 | T→G | 6.1% | N6T (AAC→ACC) | yfgM ← | ancillary SecYEG translocon subunit YfgM |
RA | NZ_CP022686 | 1,360,709 | T→G | 8.9% | intergenic (‑44/+280) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,724 | G→T | 9.9% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,392,487 | A→C | 11.3% | T43P (ACG→CCG) | lhgO → | L‑2‑hydroxyglutarate oxidase |
RA | NZ_CP022686 | 1,447,199 | A→C | 7.0% | V35G (GTA→GGA) | hycI ← | hydrogenase maturation peptidase HycI |
RA | NZ_CP022686 | 1,447,975 | C→A | 7.1% | Q165H (CAG→CAT) | hycG ← | formate hydrogenlyase subunit HycG |
RA | NZ_CP022686 | 1,479,607 | A→C | 15.7% | intergenic (‑16/+177) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
RA | NZ_CP022686 | 1,504,250 | T→G | 12.8% | V229V (GTT→GTG) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,257 | A→T | 8.1% | S232C (AGT→TGT) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,339 | C→A | 17.8% | P259Q (CCA→CAA) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,504,351 | A→C | 8.6% | N263T (AAC→ACC) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,535,307 | A→C | 9.4% | D16A (GAC→GCC) | fucI → | L‑fucose isomerase |
RA | NZ_CP022686 | 1,535,366 | G→C | 21.4% | A36P (GCC→CCC) | fucI → | L‑fucose isomerase |
RA | NZ_CP022686 | 1,564,543 | A→C | 7.1% | S182R (AGC→CGC) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,564,569 | A→C | 9.8% | K190N (AAA→AAC) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,632,480 | T→G | 8.3% | V159G (GTC→GGC) | arcC → | carbamate kinase |
RA | NZ_CP022686 | 1,640,925 | A→C | 10.8% | R7S (AGA→AGC) | ygfM → | molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
RA | NZ_CP022686 | 1,674,231 | A→T | 11.9% | V186E (GTA→GAA) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,244 | T→G | 14.4% | T182P (ACC→CCC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,248 | T→G | 11.9% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,705,974 | T→G | 15.6% | intergenic (‑584/‑71) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,007 | G→C | 6.9% | intergenic (‑617/‑38) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,023 | T→A | 6.1% | intergenic (‑633/‑22) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,032 | G→T | 11.1% | intergenic (‑642/‑13) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,041 | A→T | 7.7% | intergenic (‑651/‑4) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,706,767 | T→G | 5.6% | G241G (GGT→GGG) | metK → | methionine adenosyltransferase |
RA | NZ_CP022686 | 1,785,695 | T→G | 8.7% | pseudogene (16/186 nt) | CIW80_RS08875 ← | zinc‑ribbon domain‑containing protein |
RA | NZ_CP022686 | 1,828,113 | A→C | 9.0% | P84P (CCT→CCG) | gspK ← | type II secretion system minor pseudopilin GspK |
RA | NZ_CP022686 | 1,829,636 | T→G | 5.8% | T87P (ACC→CCC) | gspH ← | type II secretion system minor pseudopilin GspH |
RA | NZ_CP022686 | 1,860,077 | G→T | 13.1% | G116G (GGC→GGA) | CIW80_RS09255 ← | YtfJ family protein |
RA | NZ_CP022686 | 1,925,332 | A→C | 10.7% | N151T (AAC→ACC) | ygiB → | DUF1190 family protein |
RA | NZ_CP022686 | 1,925,365 | A→C | 8.9% | D162A (GAC→GCC) | ygiB → | DUF1190 family protein |
RA | NZ_CP022686 | 1,965,173 | A→C | 9.2% | R86R (CGT→CGG) | nfeF ← | NADPH‑dependent ferric chelate reductase |
RA | NZ_CP022686 | 2,016,170 | A→C | 10.5% | noncoding (104/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,016,175 | T→G | 7.1% | noncoding (99/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,016,224 | G→C | 6.2% | noncoding (50/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,037,521 | A→C | 6.8% | T508P (ACC→CCC) | lpoA → | penicillin‑binding protein activator LpoA |
RA | NZ_CP022686 | 2,049,089 | T→G | 15.4% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,049,094 | G→T | 7.4% | T254T (ACC→ACA) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,049,097 | A→T | 12.1% | R253R (CGT→CGA) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,070,788 | A→C | 9.4% | intergenic (‑80/‑169) | greA ← / → dacB | transcription elongation factor GreA/serine‑type D‑Ala‑D‑Ala carboxypeptidase |
RA | NZ_CP022686 | 2,076,554 | T→G | 12.5% | intergenic (+24/‑209) | ispB → / → sfsB | octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB |
RA | NZ_CP022686 | 2,110,457 | T→G | 9.1% | K12N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,110,465 | T→G | 8.0% | T10P (ACC→CCC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,123,234 | T→A | 7.4% | Y210F (TAT→TTT) | tldD ← | metalloprotease TldD |
RA | NZ_CP022686 | 2,130,829 | A→C | 12.2% | V237G (GTT→GGT) | mreC ← | rod shape‑determining protein MreC |
RA | NZ_CP022686 | 2,148,109 | T→G | 9.4% | V124G (GTG→GGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,115 | T→G | 10.9% | L126R (CTG→CGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,118 | C→G | 10.8% | A127G (GCG→GGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,141 | T→G | 7.7% | F135V (TTC→GTC) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,172,177 | A→C | 7.7% | G87G (GGT→GGG) | smg ← | DUF494 family protein Smg |
RA | NZ_CP022686 | 2,179,888 | C→A | 7.7% | E32* (GAA→TAA) | rplQ ← | 50S ribosomal protein L17 |
RA | NZ_CP022686 | 2,179,916 | G→T | 6.8% | R22R (CGC→CGA) | rplQ ← | 50S ribosomal protein L17 |
RA | NZ_CP022686 | 2,185,075 | A→C | 11.6% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,284,668 | A→C | 6.7% | R207R (CGT→CGG) | malQ ← | 4‑alpha‑glucanotransferase |
RA | NZ_CP022686 | 2,310,429 | T→G | 6.2% | N248T (AAC→ACC) | glgP ← | glycogen phosphorylase |
RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,357,291 | A→C | 8.0% | N26T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,357,322 | T→A | 11.8% | S36R (AGT→AGA) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,357,324 | A→C | 17.5% | N37T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,393,013 | T→G | 6.4% | intergenic (+151/‑2) | slp → / → dctR | outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator |
RA | NZ_CP022686 | 2,398,885 | A→C | 9.2% | S203R (AGC→CGC) | hutW → | heme anaerobic degradation radical SAM methyltransferase ChuW/HutW |
RA | NZ_CP022686 | 2,398,896 | A→C | 10.6% | K206N (AAA→AAC) | hutW → | heme anaerobic degradation radical SAM methyltransferase ChuW/HutW |
RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
RA | NZ_CP022686 | 2,421,692 | G→C | 7.5% | D327H (GAC→CAC) | yhjD → | inner membrane protein YhjD |
RA | NZ_CP022686 | 2,448,912 | G→T | 9.8% | A180S (GCG→TCG) | yhjV → | aromatic amino acid transport family protein |
RA | NZ_CP022686 | 2,460,526 | A→C | 9.1% | V18G (GTG→GGG) | yhjY ← | autotransporter outer membrane beta‑barrel domain‑containing protein |
RA | NZ_CP022686 | 2,460,535 | A→C | 11.0% | L15R (CTT→CGT) | yhjY ← | autotransporter outer membrane beta‑barrel domain‑containing protein |
RA | NZ_CP022686 | 2,470,422 | T→G | 10.0% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
RA | NZ_CP022686 | 2,516,216 | T→G | 8.4% | S542S (TCT→TCG) | upaG → | trimeric autotransporter adhesin UpaG |
RA | NZ_CP022686 | 2,516,577 | A→G | 8.0% | T663A (ACA→GCA) | upaG → | trimeric autotransporter adhesin UpaG |
RA | NZ_CP022686 | 2,546,381 | A→C | 11.5% | A99A (GCT→GCG) | waaG ← | glycosyltransferase family 4 protein |
RA | NZ_CP022686 | 2,546,400 | A→C | 11.5% | L93R (CTG→CGG) | waaG ← | glycosyltransferase family 4 protein |
RA | NZ_CP022686 | 2,546,423 | A→C | 13.1% | V85V (GTT→GTG) | waaG ← | glycosyltransferase family 4 protein |
RA | NZ_CP022686 | 2,550,305 | T→G | 5.4% | K151N (AAA→AAC) | mutM ← | bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase |
RA | NZ_CP022686 | 2,572,705 | A→C | 10.8% | G191G (GGT→GGG) | CIW80_RS12885 ← | ROK family protein |
JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
RA | NZ_CP022686 | 2,617,885 | T→G | 9.5% | K190N (AAA→AAC) | ilvB ← | acetolactate synthase large subunit |
RA | NZ_CP022686 | 2,717,400 | T→G | 5.8% | L101V (TTA→GTA) | ilvA → | threonine ammonia‑lyase, biosynthetic |
RA | NZ_CP022686 | 2,733,571 | T→G | 10.5% | F53V (TTT→GTT) | rffG → | dTDP‑glucose 4,6‑dehydratase |
RA | NZ_CP022686 | 2,733,577 | T→G | 9.8% | F55V (TTT→GTT) | rffG → | dTDP‑glucose 4,6‑dehydratase |
RA | NZ_CP022686 | 2,736,578 | C→A | 10.5% | T180T (ACC→ACA) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,736,585 | T→A | 9.6% | Y183N (TAC→AAC) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,743,563 | T→G | 9.0% | noncoding (65/87 nt) | CIW80_RS13750 → | tRNA‑Leu |
RA | NZ_CP022686 | 2,769,196 | A→C | 17.2% | F47C (TTT→TGT) | rarD ← | EamA family transporter RarD |
RA | NZ_CP022686 | 2,810,051 | G→C | 6.5% | E246Q (GAA→CAA) | tatC → | Sec‑independent protein translocase subunit TatC |
RA | NZ_CP022686 | 2,821,644 | A→C | 8.1% | intergenic (+100/‑278) | hemG → / → CIW80_RS14130 | menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA |
RA | NZ_CP022686 | 2,868,740 | T→G | 22.0% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,868,753 | T→G | 11.4% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,904,856 | T→G | 6.1% | K237N (AAA→AAC) | ftsN ← | cell division protein FtsN |
RA | NZ_CP022686 | 2,920,385 | C→A | 5.2% | A54E (GCA→GAA) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
RA | NZ_CP022686 | 2,920,411 | G→T | 8.3% | E63* (GAA→TAA) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
RA | NZ_CP022686 | 2,933,245 | A→C | 10.2% | S26R (AGC→CGC) | frwB → | PTS fructose‑like transporter subunit IIB |
RA | NZ_CP022686 | 2,961,318 | A→C | 6.3% | intergenic (+96/‑277) | murI → / → CIW80_RS14765 | glutamate racemase/16S ribosomal RNA |
RA | NZ_CP022686 | 2,989,627 | T→G | 9.4% | Q39H (CAA→CAC) | thiC ← | phosphomethylpyrimidine synthase ThiC |
RA | NZ_CP022686 | 2,994,693 | T→G | 8.8% | W162G (TGG→GGG) | yjaH → | DUF1481 domain‑containing protein |
RA | NZ_CP022686 | 3,020,229 | T→G | 14.5% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
RA | NZ_CP022686 | 3,080,461 | T→G | 7.5% | R158R (CGT→CGG) | CIW80_RS15320 → | Ldh family oxidoreductase |
RA | NZ_CP022686 | 3,090,101 | A→C | 8.3% | V9G (GTT→GGT) | yjcB ← | YjcB family protein |
RA | NZ_CP022686 | 3,136,940 | T→G | 12.5% | D161A (GAC→GCC) | phnN ← | ribose 1,5‑bisphosphokinase |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,225,449 | T→G | 5.7% | V162G (GTC→GGC) | rnr → | ribonuclease R |
RA | NZ_CP022686 | 3,252,190 | T→G | 14.5% | K221N (AAA→AAC) | CIW80_RS16225 ← | enoyl‑CoA hydratase/isomerase family protein |
RA | NZ_CP022686 | 3,252,198 | C→A | 16.9% | E219* (GAA→TAA) | CIW80_RS16225 ← | enoyl‑CoA hydratase/isomerase family protein |
RA | NZ_CP022686 | 3,265,084 | T→G | 17.4% | N77T (AAC→ACC) | ytfJ ← | YtfJ family protein |
RA | NZ_CP022686 | 3,348,371 | T→G | 7.4% | intergenic (‑187/‑13) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,363,154 | A→C | 7.8% | K21N (AAA→AAC) | CIW80_RS16820 → | Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase |
RA | NZ_CP022686 | 3,390,629 | C→A | 10.0% | W138C (TGG→TGT) | sgcX ← | M42 family metallopeptidase |
RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
RA | NZ_CP022686 | 3,461,221 | T→G | 14.3% | intergenic (‑30/+5) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
RA | NZ_CP022686 | 3,461,229 | T→G | 23.8% | noncoding (84/87 nt) | CIW80_RS17340 ← | tRNA‑Leu |
RA | NZ_CP022686 | 3,469,923 | T→G | 8.0% | E122D (GAA→GAC) | yjjW ← | YjjW family glycine radical enzyme activase |
RA | NZ_CP022686 | 3,496,452 | A→C | 12.4% | I273L (ATC→CTC) | thrA → | bifunctional aspartate kinase/homoserine dehydrogenase I |
RA | NZ_CP022686 | 3,516,093 | T→G | 6.9% | V219G (GTG→GGG) | nhaA → | Na+/H+ antiporter NhaA |
MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | 100% | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
RA | NZ_CP022686 | 3,597,494 | T→G | 9.3% | F281V (TTT→GTT) | murG → | undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase |
RA | NZ_CP022686 | 3,634,591 | G→T | 10.6% | D260Y (GAT→TAT) | acnB → | bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase |
RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,661,518 | C→A | 13.5% | C9F (TGC→TTC) | pcnB ← | polynucleotide adenylyltransferase PcnB |
RA | NZ_CP022686 | 3,703,648 | A→C | 7.2% | T120P (ACG→CCG) | fabZ → | 3‑hydroxyacyl‑ACP dehydratase FabZ |
RA | NZ_CP022686 | 3,734,367 | T→G | 15.1% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,734,380 | T→G | 11.5% | L229R (CTC→CGC) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,770,524 | T→G | 7.3% | intergenic (‑260/+203) | CIW80_RS26265 ← / ← CIW80_RS18890 | ATP‑binding protein/hypothetical protein |
RA | NZ_CP022686 | 3,770,535 | C→G | 6.6% | intergenic (‑271/+192) | CIW80_RS26265 ← / ← CIW80_RS18890 | ATP‑binding protein/hypothetical protein |
RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
RA | NZ_CP022686 | 3,797,481 | T→G | 15.9% | L130V (TTA→GTA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,798,080 | T→G | 8.4% | R329R (CGT→CGG) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,798,083 | C→A | 8.7% | I330I (ATC→ATA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,798,092 | C→A | 10.3% | F333L (TTC→TTA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,800,981 | A→C | 8.8% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
RA | NZ_CP022686 | 3,806,262 | G→T | 8.5% | D1585Y (GAT→TAT) | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
RA | NZ_CP022686 | 3,806,265 | T→A | 10.3% | Y1586N (TAC→AAC) ‡ | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
RA | NZ_CP022686 | 3,806,266 | A→C | 10.5% | Y1586S (TAC→TCC) ‡ | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
RA | NZ_CP022686 | 3,852,459 | T→G | 5.0% | N178T (AAC→ACC) | phoE ← | phosphoporin PhoE |
RA | NZ_CP022686 | 3,868,846 | T→G | 8.5% | T383P (ACG→CCG) | ecpC ← | fimbrial usher EcpC |
RA | NZ_CP022686 | 3,885,457 | A→C | 9.3% | N1404T (AAC→ACC) | fdeC → | inverse autotransporter adhesin FdeC |
RA | NZ_CP022686 | 3,925,283 | G→C | 8.6% | S154T (AGC→ACC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,925,289 | A→C | 14.1% | N156T (AAC→ACC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,925,309 | G→T | 13.4% | E163* (GAA→TAA) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,932,443 | C→A | 15.5% | E184* (GAA→TAA) | lacA ← | galactoside O‑acetyltransferase |
RA | NZ_CP022686 | 3,935,324 | T→G | 13.1% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,949,969 | T→G | 9.6% | D141A (GAC→GCC) | hemB ← | porphobilinogen synthase |
RA | NZ_CP022686 | 3,981,933 | T→G | 13.4% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
RA | NZ_CP022686 | 3,984,841 | T→G | 7.8% | R67R (CGT→CGG) | queA → | tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA |
RA | NZ_CP022686 | 4,000,557 | G→T | 8.9% | A51A (GCC→GCA) | xseB ← | exodeoxyribonuclease VII small subunit |
RA | NZ_CP022686 | 4,014,238 | C→G | 16.5% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,242 | A→T | 12.4% | intergenic (+88/‑256) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,032,292 | A→C | 18.7% | E106D (GAA→GAC) | glnK → | P‑II family nitrogen regulator |
RA | NZ_CP022686 | 4,067,840 | A→C | 5.4% | K7N (AAA→AAC) | ybaQ → | HigA family addiction module antitoxin |
RA | NZ_CP022686 | 4,133,839 | T→G | 10.1% | K117T (AAA→ACA) | ybdF ← | MmcQ/YjbR family DNA‑binding protein |
RA | NZ_CP022686 | 4,234,396 | C→A | 8.0% | intergenic (+96/‑346) | glnS → / → chiP | glutamine‑‑tRNA ligase/chitoporin |
RA | NZ_CP022686 | 4,287,491 | T→G | 7.8% | noncoding (4/76 nt) | CIW80_RS21480 → | tRNA‑Lys |
RA | NZ_CP022686 | 4,287,499 | T→G | 9.9% | noncoding (12/76 nt) | CIW80_RS21480 → | tRNA‑Lys |
RA | NZ_CP022686 | 4,290,599 | T→G | 7.0% | T121P (ACC→CCC) | ybgS ← | YbgS‑like family protein |
RA | NZ_CP022686 | 4,331,940 | A→C | 20.3% | F289V (TTT→GTT) | ybhR ← | ABC transporter permease |
RA | NZ_CP022686 | 4,368,341 | T→G | 14.9% | D47A (GAC→GCC) | ybiY ← | glycyl‑radical enzyme activating protein |
RA | NZ_CP022686 | 4,427,643 | A→C | 10.0% | T4P (ACG→CCG) | clpS → | ATP‑dependent Clp protease adapter ClpS |
RA | NZ_CP022686 | 4,440,296 | G→C | 7.5% | V785L (GTT→CTT) ‡ | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,440,297 | T→A | 7.6% | V785D (GTT→GAT) ‡ | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,440,324 | C→A | 13.9% | P794Q (CCG→CAG) | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,440,334 | A→C | 16.1% | Q797H (CAA→CAC) | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,485,654 | T→G | 6.8% | V152G (GTT→GGT) | gloC → | hydroxyacylglutathione hydrolase GloC |
RA | NZ_CP022686 | 4,562,550 | T→G | 17.5% | N140H (AAC→CAC) | putA ← | trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase |
RA | NZ_CP022686 | 4,616,703 | T→G | 12.0% | intergenic (+185/+348) | CIW80_RS23075 → / ← CIW80_RS26560 | hypothetical protein/ATP‑binding protein |
RA | NZ_CP022686 | 4,632,182 | T→G | 9.3% | R276R (CGT→CGG) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
RA | NZ_CP022686 | 4,642,951 | A→C | 7.5% | N732T (AAC→ACC) | sfaF → | S/F1C fimbrial usher SfaF/FocD |
RA | NZ_CP022686 | 4,661,469 | C→A | 11.0% | pseudogene (569/655 nt) | CIW80_RS26570 → | hypothetical protein |
RA | NZ_CP022686 | 4,678,041 | A→C | 14.6% | intergenic (+39/‑82) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,712,764 | G→C | 18.2% | A52G (GCT→GGT) | yceB ← | lipoprotein |
RA | NZ_CP022686 | 4,764,882 | T→G | 7.3% | intergenic (‑62/‑199) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,780,176 | A→C | 13.6% | intergenic (‑75/+95) | phoP ← / ← purB | two‑component system response regulator PhoP/adenylosuccinate lyase |
RA | NZ_CP022686 | 4,789,702 | G→T | 5.9% | T67T (ACC→ACA) | CIW80_RS24095 ← | DUF1828 domain‑containing protein |
RA | NZ_CP022686 | 4,814,961 | T→G | 9.4% | F61C (TTT→TGT) | CIW80_RS24295 → | phage tail protein |
RA | NZ_CP022686 | 4,814,990 | T→G | 8.2% | F71V (TTT→GTT) | CIW80_RS24295 → | phage tail protein |
RA | NZ_CP022686 | 4,815,879 | T→G | 9.8% | F23V (TTC→GTC) | CIW80_RS24305 → | C40 family peptidase |
RA | NZ_CP022686 | 4,815,886 | T→G | 7.9% | V25G (GTG→GGG) | CIW80_RS24305 → | C40 family peptidase |
MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | 100% | [umuD]–[umuC] | [umuD], [umuC] | |
RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | NZ_CP022686 | 4,905,170 | T→G | 11.4% | V99G (GTT→GGT) | CIW80_RS24815 → | coenzyme F390 synthetase |
RA | NZ_CP022686 | 4,918,837 | T→G | 7.5% | K390N (AAA→AAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
RA | NZ_CP022686 | 4,918,873 | T→G | 9.7% | Q378H (CAA→CAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
RA | NZ_CP022686 | 4,951,545 | A→C | 11.8% | S47R (AGT→CGT) | topA → | type I DNA topoisomerase |
RA | NZ_CP022686 | 4,980,895 | A→C | 11.3% | C101W (TGT→TGG) | sapD ← | peptide ABC transporter ATP‑binding protein SapD |
RA | NZ_CP022686 | 5,043,558 | T→C | 5.7% | S1063S (AGT→AGC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,043,855 | T→A | 13.7% | G1162G (GGT→GGA) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,043,951 | T→C | 9.5% | G1194G (GGT→GGC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,044,182 | T→A | 11.4% | G1271G (GGT→GGA) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 667–2618 | 3050–2957 | 340–2384 | 5954 [5438] | [5470] 5732 | JNG15_RS00010–mobA | JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4740–4809 | 5478–5214 | 406–739 | 4832 [4568] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | 262466 = | 1 (0.000) | 221 (0.970) | 86/250 | NT | 99.5% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
* | ? | NZ_CP022686 | = 262468 | 1 (0.000) | 201 (0.880) | 78/250 | NT | 99.5% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase | |||||
* | ? | NZ_CP022686 | = 3844425 | 3 (0.010) | 195 (0.860) | 87/248 | NT | 98.5% | coding (1/1056 nt) | dinB | DNA polymerase IV |
? | NZ_CP022686 | 3845462 = | 3 (0.010) | coding (1038/1056 nt) | dinB | DNA polymerase IV | |||||
* | ? | NZ_CP022686 | 4514374 = | 214 (0.940) | 6 (0.000) +AGCT |
4/242 | NT | 5.3% | coding (59/1053 nt) | ompA | porin OmpA |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3609 = | 8 (0.000) | coding (1/399 nt) | IL2wt no signal |