breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_CP022686 | 196,840 | A→C | 8.7% | N132T (AAC→ACC) | dtpA → | dipeptide/tripeptide permease DtpA |
RA | NZ_CP022686 | 222,028 | T→G | 5.7% | E167D (GAA→GAC) | ribE ← | riboflavin synthase |
RA | NZ_CP022686 | 263,831 | A→C | 14.2% | intergenic (+38/+19) | fadK → / ← ppsA | medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase |
RA | NZ_CP022686 | 298,027 | A→C | 10.4% | F60C (TTT→TGT) | chbC ← | PTS N,N'‑diacetylchitobiose transporter subunit IIC |
RA | NZ_CP022686 | 349,106 | A→C | 13.8% | T607P (ACC→CCC) | yeaG → | protein kinase YeaG |
RA | NZ_CP022686 | 360,227 | T→G | 25.6% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 372,574 | A→C | 14.4% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 512,717 | T→G | 21.5% | intergenic (‑217/+13) | sdiA ← / ← yecC | transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC |
RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
RA | NZ_CP022686 | 601,295 | T→G | 16.5% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 601,302 | T→G | 16.0% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
RA | NZ_CP022686 | 605,525 | A→C | 6.7% | N3017T (AAC→ACC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
RA | NZ_CP022686 | 636,713 | A→C | 10.3% | R452R (CGT→CGG) | clbM ← | precolibactin export MATE transporter ClbM |
RA | NZ_CP022686 | 651,848 | A→C | 13.7% | R238R (CGT→CGG) | clbJ ← | colibactin non‑ribosomal peptide synthetase ClbJ |
RA | NZ_CP022686 | 693,021 | A→C | 7.3% | T121P (ACG→CCG) | CIW80_RS03550 → | YaiO family outer membrane beta‑barrel protein |
RA | NZ_CP022686 | 694,457 | G→T | 11.8% | E8* (GAA→TAA) | CIW80_RS03560 → | PfkB family carbohydrate kinase |
RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 764,997 | T→G | 17.3% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 765,001 | A→T | 11.3% | Y40N (TAC→AAC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 829,441 | C→A | 24.7% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,448 | T→A | 11.4% | intergenic (‑37/+190) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,452 | G→T | 14.0% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,467 | C→A | 16.1% | intergenic (‑56/+171) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 837,555 | T→G | 13.7% | intergenic (‑128/+95) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
RA | NZ_CP022686 | 837,561 | G→T | 12.8% | intergenic (‑134/+89) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
RA | NZ_CP022686 | 881,739 | A→C | 18.0% | intergenic (+254/+133) | yohP → / ← dusC | protein YohP/tRNA dihydrouridine(16) synthase DusC |
RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 948,027 | A→T | 10.2% | intergenic (+96/‑161) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,049 | A→T | 10.3% | intergenic (+118/‑139) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,080 | G→T | 15.5% | intergenic (+149/‑108) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 948,090 | C→A | 15.1% | intergenic (+159/‑98) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
RA | NZ_CP022686 | 957,112 | T→G | 11.1% | T89P (ACC→CCC) | ccmA ← | cytochrome c biogenesis heme‑transporting ATPase CcmA |
RA | NZ_CP022686 | 1,005,085 | C→A | 12.7% | T380T (ACC→ACA) | nrdA → | ribonucleoside‑diphosphate reductase subunit alpha |
RA | NZ_CP022686 | 1,112,973 | A→C | 5.6% | V127G (GTG→GGG) | fadJ ← | fatty acid oxidation complex subunit alpha FadJ |
RA | NZ_CP022686 | 1,174,272 | A→C | 18.0% | intergenic (+1/‑43) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
RA | NZ_CP022686 | 1,174,278 | T→G | 10.8% | intergenic (+7/‑37) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
RA | NZ_CP022686 | 1,197,142 | T→G | 8.9% | G74G (GGT→GGG) | murQ → | N‑acetylmuramic acid 6‑phosphate etherase |
RA | NZ_CP022686 | 1,209,917 | G→T | 11.6% | P393Q (CCA→CAA) | eutH ← | ethanolamine utilization protein EutH |
RA | NZ_CP022686 | 1,250,512 | T→G | 8.6% | G41G (GGT→GGG) | purM → | phosphoribosylformylglycinamidine cyclo‑ligase |
RA | NZ_CP022686 | 1,276,970 | A→C | 6.1% | G414G (GGT→GGG) | der ← | ribosome biogenesis GTPase Der |
RA | NZ_CP022686 | 1,325,398 | C→A | 7.8% | E260* (GAA→TAA) | qseE ← | two component system sensor histidine kinase QseE/GlrK |
RA | NZ_CP022686 | 1,360,709 | T→G | 11.0% | intergenic (‑44/+280) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,724 | G→T | 10.4% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,727 | A→T | 9.6% | intergenic (‑62/+262) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,736 | G→T | 9.3% | intergenic (‑71/+253) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,396,420 | T→G | 10.8% | intergenic (+26/‑211) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,504,310 | G→T | 13.3% | M249I (ATG→ATT) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,535,353 | G→T | 18.0% | M31I (ATG→ATT) | fucI → | L‑fucose isomerase |
RA | NZ_CP022686 | 1,547,003 | G→C | 12.5% | intergenic (+25/‑9) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,547,110 | G→C | 22.2% | intergenic (+22/‑12) | CIW80_RS07710 → / → CIW80_RS07715 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,564,573 | T→G | 10.7% | W192G (TGG→GGG) | CIW80_RS07780 → | ImcF‑related family protein |
RA | NZ_CP022686 | 1,633,846 | T→G | 35.8% | N253T (AAC→ACC) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,634,345 | A→C | 6.0% | S87A (TCA→GCA) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,640,925 | A→C | 14.4% | R7S (AGA→AGC) | ygfM → | molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
RA | NZ_CP022686 | 1,674,224 | T→G | 12.2% | K188N (AAA→AAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,231 | A→T | 9.2% | V186E (GTA→GAA) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,706,047 | G→T | 14.9% | M1M (ATG→ATT) † | metK → | methionine adenosyltransferase |
RA | NZ_CP022686 | 1,710,433 | A→C | 6.1% | intergenic (+28/‑52) | endA → / → rsmE | deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase |
RA | NZ_CP022686 | 1,716,167 | A→C | 14.6% | N49T (AAC→ACC) | rdgB → | XTP/dITP diphosphatase |
RA | NZ_CP022686 | 1,758,771 | T→G | 8.7% | intergenic (+18/‑254) | sat → / → CIW80_RS08700 | serine protease autotransporter toxin Sat/transposase |
RA | NZ_CP022686 | 1,925,332 | A→C | 12.5% | N151T (AAC→ACC) | ygiB → | DUF1190 family protein |
RA | NZ_CP022686 | 1,954,923 | T→G | 15.9% | T92P (ACG→CCG) | ttdR ← | DNA‑binding transcriptional activator TtdR |
RA | NZ_CP022686 | 2,016,160 | T→G | 6.8% | noncoding (114/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,016,170 | A→C | 9.4% | noncoding (104/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,016,225 | T→G | 8.6% | noncoding (49/377 nt) | rnpB ← | RNase P RNA component class A |
RA | NZ_CP022686 | 2,056,168 | A→C | 11.9% | G614G (GGT→GGG) | infB ← | translation initiation factor IF‑2 |
RA | NZ_CP022686 | 2,110,457 | T→G | 9.8% | K12N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
RA | NZ_CP022686 | 2,130,829 | A→C | 7.0% | V237G (GTT→GGT) | mreC ← | rod shape‑determining protein MreC |
RA | NZ_CP022686 | 2,148,122 | T→G | 8.0% | S128R (AGT→AGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,126 | A→T | 8.3% | S130C (AGT→TGT) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,141 | T→G | 8.7% | F135V (TTC→GTC) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,159,687 | T→A | 9.1% | Y172N (TAC→AAC) | CIW80_RS10780 → | amino acid ABC transporter substrate‑binding protein |
RA | NZ_CP022686 | 2,185,075 | A→C | 10.1% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
RA | NZ_CP022686 | 2,188,239 | G→T | 7.1% | intergenic (‑108/+57) | rplN ← / ← rpsQ | 50S ribosomal protein L14/30S ribosomal protein S17 |
RA | NZ_CP022686 | 2,207,122 | A→C | 9.8% | intergenic (‑146/+23) | bfd ← / ← chiA | bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme |
RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,277,483 | G→T | 7.3% | G536G (GGG→GGT) | feoB → | Fe(2+) transporter permease subunit FeoB |
RA | NZ_CP022686 | 2,277,493 | G→T | 5.7% | D540Y (GAT→TAT) | feoB → | Fe(2+) transporter permease subunit FeoB |
RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,357,291 | A→C | 9.6% | N26T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,357,324 | A→C | 22.2% | N37T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,361,489 | T→G | 15.8% | V188G (GTT→GGT) | nikA → | nickel ABC transporter substrate‑binding protein |
RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
RA | NZ_CP022686 | 2,460,576 | C→A | 13.6% | M1M (ATG→ATT) † | yhjY ← | autotransporter outer membrane beta‑barrel domain‑containing protein |
RA | NZ_CP022686 | 2,470,422 | T→G | 14.3% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
RA | NZ_CP022686 | 2,516,872 | A→C | 8.3% | K761T (AAA→ACA) | upaG → | trimeric autotransporter adhesin UpaG |
RA | NZ_CP022686 | 2,546,400 | A→C | 12.1% | L93R (CTG→CGG) | waaG ← | glycosyltransferase family 4 protein |
JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
RA | NZ_CP022686 | 2,670,064 | A→C | 8.1% | V284G (GTT→GGT) | pstC ← | phosphate ABC transporter permease PstC |
RA | NZ_CP022686 | 2,736,586 | A→C | 11.0% | Y183S (TAC→TCC) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
RA | NZ_CP022686 | 2,758,128 | A→C | 11.4% | K40N (AAA→AAC) | yifL → | lipoprotein |
RA | NZ_CP022686 | 2,769,196 | A→C | 17.8% | F47C (TTT→TGT) | rarD ← | EamA family transporter RarD |
RA | NZ_CP022686 | 2,834,886 | A→C | 8.5% | K597N (AAA→AAC) | polA → | DNA polymerase I |
RA | NZ_CP022686 | 2,868,740 | T→G | 27.9% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,868,753 | T→G | 15.3% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,904,856 | T→G | 6.1% | K237N (AAA→AAC) | ftsN ← | cell division protein FtsN |
RA | NZ_CP022686 | 2,933,753 | A→C | 6.2% | K71N (AAA→AAC) | pflD → | formate C‑acetyltransferase |
RA | NZ_CP022686 | 2,937,203 | T→G | 7.3% | T221P (ACG→CCG) | yijO ← | AraC family transcriptional regulator |
RA | NZ_CP022686 | 2,961,318 | A→C | 6.3% | intergenic (+96/‑277) | murI → / → CIW80_RS14765 | glutamate racemase/16S ribosomal RNA |
RA | NZ_CP022686 | 3,020,229 | T→G | 13.8% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
RA | NZ_CP022686 | 3,136,940 | T→G | 10.0% | D161A (GAC→GCC) | phnN ← | ribose 1,5‑bisphosphokinase |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,265,084 | T→G | 14.4% | N77T (AAC→ACC) | ytfJ ← | YtfJ family protein |
RA | NZ_CP022686 | 3,278,001 | G→T | 8.9% | E281* (GAG→TAG) | ytfQ → | galactofuranose ABC transporter substrate‑binding protein YtfQ |
RA | NZ_CP022686 | 3,281,004 | T→G | 11.5% | F75V (TTT→GTT) | yjfF → | sugar ABC transporter permease YjfF |
RA | NZ_CP022686 | 3,287,046 | A→C | 5.9% | K117N (AAA→AAC) | cybC → | cytochrome b562 |
RA | NZ_CP022686 | 3,348,371 | T→G | 6.9% | intergenic (‑187/‑13) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,387,097 | T→G | 11.0% | D92A (GAC→GCC) | sgcA ← | SgcA family PTS sugar transporter subunit IIA |
RA | NZ_CP022686 | 3,387,616 | G→T | 6.3% | I231I (ATC→ATA) | sgcQ ← | BtpA family protein SgcQ |
RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
RA | NZ_CP022686 | 3,444,704 | T→G | 13.6% | T530P (ACC→CCC) | btsT ← | pyruvate/proton symporter BtsT |
MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | 100% | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
RA | NZ_CP022686 | 3,604,565 | A→T | 17.6% | intergenic (+81/‑150) | lpxC → / → secM | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM |
RA | NZ_CP022686 | 3,652,090 | T→G | 5.9% | intergenic (‑99/+13) | panB ← / ← CIW80_RS18255 | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin |
RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,667,058 | A→C | 6.0% | D5A (GAC→GCC) | mrcB → | bifunctional glycosyl transferase/transpeptidase |
RA | NZ_CP022686 | 3,734,367 | T→G | 14.5% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,734,380 | T→G | 14.9% | L229R (CTC→CGC) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,753,184 | T→G | 7.4% | N350T (AAC→ACC) | CIW80_RS18790 ← | patatin‑like phospholipase family protein |
RA | NZ_CP022686 | 3,770,509 | A→T | 9.1% | intergenic (‑245/+218) | CIW80_RS26265 ← / ← CIW80_RS18890 | ATP‑binding protein/hypothetical protein |
RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
RA | NZ_CP022686 | 3,797,481 | T→G | 16.0% | L130V (TTA→GTA) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
RA | NZ_CP022686 | 3,800,981 | A→C | 7.7% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
MC JC | NZ_CP022686 | 3,844,426 | Δ1,036 bp | 100% | coding (2‑1037/1056 nt) | dinB → | DNA polymerase IV |
RA | NZ_CP022686 | 3,868,846 | T→G | 8.9% | T383P (ACG→CCG) | ecpC ← | fimbrial usher EcpC |
RA | NZ_CP022686 | 3,925,289 | A→C | 14.9% | N156T (AAC→ACC) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,928,345 | T→G | 9.2% | F277V (TTC→GTC) | prpE → | propionate‑‑CoA ligase |
RA | NZ_CP022686 | 3,935,324 | T→G | 14.2% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,935,367 | T→G | 8.1% | S691R (AGC→CGC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,940,724 | A→C | 11.1% | V308G (GTA→GGA) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
RA | NZ_CP022686 | 3,949,969 | T→G | 10.3% | D141A (GAC→GCC) | hemB ← | porphobilinogen synthase |
RA | NZ_CP022686 | 3,981,933 | T→G | 13.1% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
RA | NZ_CP022686 | 4,014,238 | C→G | 15.4% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,032,225 | G→T | 6.5% | G84V (GGA→GTA) | glnK → | P‑II family nitrogen regulator |
RA | NZ_CP022686 | 4,056,946 | T→G | 9.6% | V132G (GTT→GGT) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,056,975 | G→T | 9.4% | V142L (GTA→TTA) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,056,987 | G→C | 8.5% | D146H (GAC→CAC) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,254,102 | A→C | 8.6% | K53N (AAA→AAC) | ybfA → | YbfA family protein |
RA | NZ_CP022686 | 4,331,940 | A→C | 13.6% | F289V (TTT→GTT) | ybhR ← | ABC transporter permease |
RA | NZ_CP022686 | 4,587,091 | T→G | 11.3% | N609T (AAC→ACC) | CIW80_RS22915 ← | enterotoxin |
RA | NZ_CP022686 | 4,605,579 | T→G | 17.0% | T56P (ACG→CCG) | CIW80_RS23010 ← | hypothetical protein |
RA | NZ_CP022686 | 4,632,182 | T→G | 9.3% | R276R (CGT→CGG) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
RA | NZ_CP022686 | 4,632,186 | G→T | 5.4% | G278C (GGC→TGC) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
RA | NZ_CP022686 | 4,678,047 | A→C | 25.7% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,713,009 | A→C | 5.4% | intergenic (‑91/+43) | yceB ← / ← grxB | lipoprotein/glutaredoxin 2 |
RA | NZ_CP022686 | 4,764,904 | G→T | 8.0% | intergenic (‑84/‑177) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | 100% | [umuD]–[umuC] | [umuD], [umuC] | |
RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | NZ_CP022686 | 4,905,178 | T→G | 12.2% | F102V (TTC→GTC) | CIW80_RS24815 → | coenzyme F390 synthetase |
RA | NZ_CP022686 | 4,918,873 | T→G | 8.3% | Q378H (CAA→CAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
RA | NZ_CP022686 | 5,042,304 | T→C | 8.9% | N645N (AAT→AAC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,043,347 | A→G | 6.1% | N993S (AAT→AGT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,043,648 | G→C | 6.5% | V1093V (GTG→GTC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,044,122 | T→C | 5.3% | G1251G (GGT→GGC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,507 | A→C | 6.7% | intergenic (‑1711/‑24) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] | |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,762 | G→T | 14.8% | E52* (GAA→TAA) | IL2wt no signal → | |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,784 | T→G | 69.8% | L59* (TTA→TGA) | IL2wt no signal → | |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 4,019 | +CAT | 8.3% | intergenic (+12/+37) | IL2wt no signal → / ← annotated as hok_sok locus | –/– |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 189–2618 | 3084–2957 | 340–2896 | 11875 [10866] | [11280] 12201 | JNG15_RS00005–mobA | JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4746–4809 | 5478–5074 | 266–733 | 5393 [5234] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | 262466 = | 3 (0.020) | 175 (0.920) | 70/250 | NT | 98.3% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
* | ? | NZ_CP022686 | = 262468 | 3 (0.020) | 168 (0.880) | 71/250 | NT | 98.2% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase | |||||
* | ? | NZ_CP022686 | 3899666 = | 234 (1.230) | 18 (0.110) | 15/218 | NT | 8.7% | intergenic (‑40/+290) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | 3899944 = | 174 (1.050) | intergenic (‑318/+12) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
* | ? | NZ_CP022686 | = 3899681 | 231 (1.210) | 16 (0.100) | 11/218 | NT | 7.9% | intergenic (‑55/+275) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | = 3899927 | 174 (1.050) | intergenic (‑301/+29) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |