breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 196,840 A→C 8.7% N132T (AAC→ACC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 222,028 T→G 5.7% E167D (GAA→GAC ribE ← riboflavin synthase
RA NZ_CP022686 263,831 A→C 14.2% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 298,027 A→C 10.4% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,106 A→C 13.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 25.6% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 14.4% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 512,717 T→G 21.5% intergenic (‑217/+13) sdiA ← / ← yecC transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 16.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 16.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 605,525 A→C 6.7% N3017T (AAC→ACC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,713 A→C 10.3% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,848 A→C 13.7% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,021 A→C 7.3% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 694,457 G→T 11.8% E8* (GAA→TAA)  CIW80_RS03560 → PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 17.3% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 11.3% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 24.7% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 11.4% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 14.0% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 16.1% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 13.7% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,561 G→T 12.8% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 881,739 A→C 18.0% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 10.2% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,049 A→T 10.3% intergenic (+118/‑139) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 15.5% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 15.1% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 11.1% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 12.7% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,112,973 A→C 5.6% V127G (GTG→GGG)  fadJ ← fatty acid oxidation complex subunit alpha FadJ
RA NZ_CP022686 1,174,272 A→C 18.0% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,278 T→G 10.8% intergenic (+7/‑37) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,142 T→G 8.9% G74G (GGT→GGG murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,209,917 G→T 11.6% P393Q (CCA→CAA)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,250,512 T→G 8.6% G41G (GGT→GGG purM → phosphoribosylformylglycinamidine cyclo‑ligase
RA NZ_CP022686 1,276,970 A→C 6.1% G414G (GGT→GGG der ← ribosome biogenesis GTPase Der
RA NZ_CP022686 1,325,398 C→A 7.8% E260* (GAA→TAA)  qseE ← two component system sensor histidine kinase QseE/GlrK
RA NZ_CP022686 1,360,709 T→G 11.0% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 10.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,727 A→T 9.6% intergenic (‑62/+262) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 9.3% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,396,420 T→G 10.8% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,504,310 G→T 13.3% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,353 G→T 18.0% M31I (ATG→ATT fucI → L‑fucose isomerase
RA NZ_CP022686 1,547,003 G→C 12.5% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 22.2% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,573 T→G 10.7% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,846 T→G 35.8% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,634,345 A→C 6.0% S87A (TCA→GCA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 14.4% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 12.2% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 9.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,047 G→T 14.9% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,710,433 A→C 6.1% intergenic (+28/‑52) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,167 A→C 14.6% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 8.7% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,925,332 A→C 12.5% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 15.9% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,160 T→G 6.8% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 9.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 8.6% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,056,168 A→C 11.9% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,110,457 T→G 9.8% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 7.0% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,122 T→G 8.0% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,126 A→T 8.3% S130C (AGT→TGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 8.7% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,687 T→A 9.1% Y172N (TAC→AAC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,185,075 A→C 10.1% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 7.1% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,207,122 A→C 9.8% intergenic (‑146/+23) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,277,483 G→T 7.3% G536G (GGG→GGT feoB → Fe(2+) transporter permease subunit FeoB
RA NZ_CP022686 2,277,493 G→T 5.7% D540Y (GAT→TAT)  feoB → Fe(2+) transporter permease subunit FeoB
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 9.6% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 22.2% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 15.8% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 13.6% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 14.3% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,872 A→C 8.3% K761T (AAA→ACA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,400 A→C 12.1% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,670,064 A→C 8.1% V284G (GTT→GGT)  pstC ← phosphate ABC transporter permease PstC
RA NZ_CP022686 2,736,586 A→C 11.0% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,758,128 A→C 11.4% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,769,196 A→C 17.8% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,834,886 A→C 8.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 27.9% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 15.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,856 T→G 6.1% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,933,753 A→C 6.2% K71N (AAA→AAC pflD → formate C‑acetyltransferase
RA NZ_CP022686 2,937,203 T→G 7.3% T221P (ACG→CCG)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,961,318 A→C 6.3% intergenic (+96/‑277) murI → / → CIW80_RS14765 glutamate racemase/16S ribosomal RNA
RA NZ_CP022686 3,020,229 T→G 13.8% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,136,940 T→G 10.0% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 14.4% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,278,001 G→T 8.9% E281* (GAG→TAG)  ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA NZ_CP022686 3,281,004 T→G 11.5% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,287,046 A→C 5.9% K117N (AAA→AAC cybC → cytochrome b562
RA NZ_CP022686 3,348,371 T→G 6.9% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,097 T→G 11.0% D92A (GAC→GCC)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,387,616 G→T 6.3% I231I (ATC→ATA sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 13.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,565 A→T 17.6% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,652,090 T→G 5.9% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,667,058 A→C 6.0% D5A (GAC→GCC)  mrcB → bifunctional glycosyl transferase/transpeptidase
RA NZ_CP022686 3,734,367 T→G 14.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 14.9% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,753,184 T→G 7.4% N350T (AAC→ACC)  CIW80_RS18790 ← patatin‑like phospholipase family protein
RA NZ_CP022686 3,770,509 A→T 9.1% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 16.0% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 7.7% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 8.9% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,289 A→C 14.9% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 9.2% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,935,367 T→G 8.1% S691R (AGC→CGC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 11.1% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 10.3% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 13.1% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 15.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,225 G→T 6.5% G84V (GGA→GTA)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,946 T→G 9.6% V132G (GTT→GGT)  adk → adenylate kinase
RA NZ_CP022686 4,056,975 G→T 9.4% V142L (GTA→TTA)  adk → adenylate kinase
RA NZ_CP022686 4,056,987 G→C 8.5% D146H (GAC→CAC)  adk → adenylate kinase
RA NZ_CP022686 4,254,102 A→C 8.6% K53N (AAA→AAC ybfA → YbfA family protein
RA NZ_CP022686 4,331,940 A→C 13.6% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,587,091 T→G 11.3% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,605,579 T→G 17.0% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 9.3% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,186 G→T 5.4% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,047 A→C 25.7% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,713,009 A→C 5.4% intergenic (‑91/+43) yceB ← / ← grxB lipoprotein/glutaredoxin 2
RA NZ_CP022686 4,764,904 G→T 8.0% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 12.2% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,918,873 T→G 8.3% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 5,042,304 T→C 8.9% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 6.1% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.5% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,122 T→C 5.3% G1251G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,507 A→C 6.7% intergenic (‑1711/‑24) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 14.8% E52* (GAA→TAA)  IL2wt no signal →
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,784 T→G 69.8% L59* (TTA→TGA)  IL2wt no signal →
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 8.3% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3084–2957 340–2896 11875 [10866] [11280] 12201 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4746–4809 5478–5074 266–733 5393 [5234] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =3 (0.020)175 (0.920) 70/250 NT 98.3% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.020)168 (0.880) 71/250 NT 98.2% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =234 (1.230)18 (0.110) 15/218 NT 8.7% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 174 (1.050)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681231 (1.210)16 (0.100) 11/218 NT 7.9% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 174 (1.050)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA