mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP022686 | 119,184 | T→G | 9.9% | F47V (TTT→GTT) | marB → | multiple antibiotic resistance protein MarB |
| RA | NZ_CP022686 | 298,027 | A→C | 12.2% | F60C (TTT→TGT) | chbC ← | PTS N,N'‑diacetylchitobiose transporter subunit IIC |
| RA | NZ_CP022686 | 350,056 | A→C | 17.6% | K241T (AAA→ACA) | yeaH → | YeaH/YhbH family protein |
| RA | NZ_CP022686 | 360,227 | T→G | 15.7% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
| RA | NZ_CP022686 | 361,672 | T→G | 15.5% | intergenic (+82/+11) | CIW80_RS26330 → / ← rnd | isocitrate/isopropylmalate family dehydrogenase/ribonuclease D |
| RA | NZ_CP022686 | 372,574 | A→C | 16.6% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
| RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
| RA | NZ_CP022686 | 496,135 | A→C | 11.0% | V382V (GTT→GTG) | otsA ← | alpha,alpha‑trehalose‑phosphate synthase |
| RA | NZ_CP022686 | 512,717 | T→G | 20.4% | intergenic (‑217/+13) | sdiA ← / ← yecC | transcriptional regulator SdiA/L‑cystine ABC transporter ATP‑binding protein YecC |
| RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
| RA | NZ_CP022686 | 601,295 | T→G | 21.0% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| RA | NZ_CP022686 | 601,302 | T→G | 21.6% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| RA | NZ_CP022686 | 605,525 | A→C | 7.5% | N3017T (AAC→ACC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| RA | NZ_CP022686 | 606,995 | A→C | 9.4% | R344S (AGA→AGC) | ybtU → | yersiniabactin biosynthesis oxidoreductase YbtU |
| MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
| RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
| RA | NZ_CP022686 | 765,001 | A→T | 11.4% | Y40N (TAC→AAC) | wcaM ← | colanic acid biosynthesis protein WcaM |
| RA | NZ_CP022686 | 765,016 | C→A | 8.0% | V35F (GTT→TTT) | wcaM ← | colanic acid biosynthesis protein WcaM |
| RA | NZ_CP022686 | 779,223 | A→C | 5.5% | G42G (GGT→GGG) | wcaE ← | colanic acid biosynthesis glycosyltransferase WcaE |
| RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
| RA | NZ_CP022686 | 829,441 | C→A | 24.9% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 829,448 | T→A | 9.8% | intergenic (‑37/+190) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 829,452 | G→T | 19.2% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 859,999 | T→G | 8.2% | A620A (GCT→GCG) | yehM → | DUF5682 family protein |
| RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
| RA | NZ_CP022686 | 1,005,085 | C→A | 13.9% | T380T (ACC→ACA) | nrdA → | ribonucleoside‑diphosphate reductase subunit alpha |
| RA | NZ_CP022686 | 1,047,897 | T→G | 7.7% | intergenic (‑127/+37) | nuoM ← / ← nuoL | NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L |
| RA | NZ_CP022686 | 1,171,408 | C→G | 5.7% | intergenic (‑26/+14) | CIW80_RS05830 ← / ← CIW80_RS05835 | tRNA‑Ala/tRNA‑Ala |
| RA | NZ_CP022686 | 1,174,416 | A→T | 9.5% | intergenic (+26/‑21) | CIW80_RS05860 → / → CIW80_RS05865 | tRNA‑Val/tRNA‑Val |
| RA | NZ_CP022686 | 1,197,145 | T→G | 10.5% | R75R (CGT→CGG) | murQ → | N‑acetylmuramic acid 6‑phosphate etherase |
| RA | NZ_CP022686 | 1,360,724 | G→T | 11.0% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,370,568 | G→T | 9.5% | V95V (GTC→GTA) | rluD ← | 23S rRNA pseudouridine(1911/1915/1917) synthase RluD |
| RA | NZ_CP022686 | 1,424,342 | C→G | 8.1% | intergenic (‑38/+25) | CIW80_RS07085 ← / ← CIW80_RS07090 | tRNA‑Arg/tRNA‑Arg |
| RA | NZ_CP022686 | 1,479,607 | A→C | 14.0% | intergenic (‑16/+177) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
| RA | NZ_CP022686 | 1,518,282 | T→G | 8.8% | T281P (ACC→CCC) | gudX ← | enolase C‑terminal domain‑like protein |
| RA | NZ_CP022686 | 1,535,366 | G→C | 17.8% | A36P (GCC→CCC) | fucI → | L‑fucose isomerase |
| RA | NZ_CP022686 | 1,547,000 | G→C | 17.1% | intergenic (+22/‑12) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
| RA | NZ_CP022686 | 1,547,008 | G→C | 14.4% | intergenic (+30/‑4) | CIW80_RS07705 → / → CIW80_RS07710 | tRNA‑Met/tRNA‑Met |
| RA | NZ_CP022686 | 1,558,395 | A→C | 8.3% | K464N (AAA→AAC) | vgrG → | type VI secretion system Vgr family protein |
| RA | NZ_CP022686 | 1,564,569 | A→C | 8.3% | K190N (AAA→AAC) | CIW80_RS07780 → | ImcF‑related family protein |
| RA | NZ_CP022686 | 1,597,235 | T→G | 8.2% | N19H (AAC→CAC) | thyA ← | thymidylate synthase |
| RA | NZ_CP022686 | 1,632,501 | T→G | 9.7% | V166G (GTT→GGT) | arcC → | carbamate kinase |
| RA | NZ_CP022686 | 1,640,925 | A→C | 9.5% | R7S (AGA→AGC) | ygfM → | molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
| RA | NZ_CP022686 | 1,674,231 | A→T | 9.7% | V186E (GTA→GAA) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,674,244 | T→G | 13.5% | T182P (ACC→CCC) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,674,248 | T→G | 9.7% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,705,974 | T→G | 9.7% | intergenic (‑584/‑71) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,705,997 | C→A | 8.3% | intergenic (‑607/‑48) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,805,056 | A→C | 7.6% | pseudogene (337/934 nt) | CIW80_RS09020 → | nucleotidyl transferase AbiEii/AbiGii toxin family protein |
| RA | NZ_CP022686 | 1,832,023 | T→G | 13.5% | N355T (AAC→ACC) | gspE ← | type II secretion system ATPase GspE |
| RA | NZ_CP022686 | 1,860,077 | G→T | 13.9% | G116G (GGC→GGA) | CIW80_RS09255 ← | YtfJ family protein |
| RA | NZ_CP022686 | 1,954,923 | T→G | 17.9% | T92P (ACG→CCG) | ttdR ← | DNA‑binding transcriptional activator TtdR |
| RA | NZ_CP022686 | 2,016,175 | T→G | 8.5% | noncoding (99/377 nt) | rnpB ← | RNase P RNA component class A |
| RA | NZ_CP022686 | 2,027,390 | A→C | 6.4% | K80N (AAA→AAC) | agaE → | PTS N‑acetylgalactosamine transporter subunit IID |
| RA | NZ_CP022686 | 2,049,089 | T→G | 15.8% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase DeaD |
| RA | NZ_CP022686 | 2,110,457 | T→G | 11.7% | K12N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
| RA | NZ_CP022686 | 2,110,461 | A→T | 7.0% | V11E (GTA→GAA) | rplM ← | 50S ribosomal protein L13 |
| RA | NZ_CP022686 | 2,110,465 | T→G | 9.8% | T10P (ACC→CCC) | rplM ← | 50S ribosomal protein L13 |
| RA | NZ_CP022686 | 2,130,829 | A→C | 11.8% | V237G (GTT→GGT) | mreC ← | rod shape‑determining protein MreC |
| RA | NZ_CP022686 | 2,148,118 | C→G | 11.8% | A127G (GCG→GGG) | panF → | sodium/pantothenate symporter |
| RA | NZ_CP022686 | 2,148,136 | T→G | 9.3% | V133G (GTT→GGT) | panF → | sodium/pantothenate symporter |
| RA | NZ_CP022686 | 2,185,075 | A→C | 9.7% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
| RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
| RA | NZ_CP022686 | 2,215,206 | C→A | 7.3% | A41S (GCT→TCT) | tusC ← | sulfurtransferase complex subunit TusC |
| RA | NZ_CP022686 | 2,226,139 | A→C | 9.0% | D9A (GAC→GCC) | crp → | cAMP‑activated global transcriptional regulator CRP |
| RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
| RA | NZ_CP022686 | 2,358,471 | G→T | 5.7% | H186N (CAC→AAC) | yhhS ← | MFS transporter |
| RA | NZ_CP022686 | 2,361,501 | C→A | 9.5% | S192* (TCG→TAG) | nikA → | nickel ABC transporter substrate‑binding protein |
| RA | NZ_CP022686 | 2,394,302 | A→T | 10.6% | F110Y (TTC→TAC) | chuS ← | hematinate‑forming heme oxygenase ChuS |
| RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
| RA | NZ_CP022686 | 2,460,579 | T→A | 16.5% | intergenic (‑1/‑157) | yhjY ← / → tag | autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I |
| RA | NZ_CP022686 | 2,470,422 | T→G | 11.9% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
| RA | NZ_CP022686 | 2,516,873 | A→C | 7.6% | K761N (AAA→AAC) | upaG → | trimeric autotransporter adhesin UpaG |
| RA | NZ_CP022686 | 2,516,993 | T→G | 14.0% | G801G (GGT→GGG) | upaG → | trimeric autotransporter adhesin UpaG |
| RA | NZ_CP022686 | 2,546,423 | A→C | 9.4% | V85V (GTT→GTG) | waaG ← | glycosyltransferase family 4 protein |
| JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
| RA | NZ_CP022686 | 2,758,113 | A→C | 6.9% | A35A (GCA→GCC) | yifL → | lipoprotein |
| RA | NZ_CP022686 | 2,758,144 | A→C | 11.5% | T46P (ACG→CCG) | yifL → | lipoprotein |
| RA | NZ_CP022686 | 2,761,532 | A→C | 10.6% | P21P (CCA→CCC) | uvrD → | DNA helicase II |
| RA | NZ_CP022686 | 2,868,740 | T→G | 17.2% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
| RA | NZ_CP022686 | 2,920,428 | A→C | 9.3% | K68N (AAA→AAC) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| RA | NZ_CP022686 | 2,955,291 | T→G | 6.7% | R218R (CGT→CGG) | CIW80_RS14735 → | citrate‑proton symporter |
| RA | NZ_CP022686 | 2,961,318 | A→C | 8.6% | intergenic (+96/‑277) | murI → / → CIW80_RS14765 | glutamate racemase/16S ribosomal RNA |
| RA | NZ_CP022686 | 2,977,481 | T→G | 6.4% | V558G (GTA→GGA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| RA | NZ_CP022686 | 3,013,752 | T→G | 8.9% | V471G (GTG→GGG) | aceK → | bifunctional isocitrate dehydrogenase kinase/phosphatase |
| RA | NZ_CP022686 | 3,020,229 | T→G | 15.5% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
| RA | NZ_CP022686 | 3,136,935 | T→G | 11.1% | S163R (AGC→CGC) | phnN ← | ribose 1,5‑bisphosphokinase |
| RA | NZ_CP022686 | 3,136,940 | T→G | 9.7% | D161A (GAC→GCC) | phnN ← | ribose 1,5‑bisphosphokinase |
| RA | NZ_CP022686 | 3,150,950 | A→C | 7.2% | Q252H (CAA→CAC) | rdcB → | YjcZ‑like family protein |
| JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
| RA | NZ_CP022686 | 3,252,190 | T→G | 15.2% | K221N (AAA→AAC) | CIW80_RS16225 ← | enoyl‑CoA hydratase/isomerase family protein |
| RA | NZ_CP022686 | 3,348,359 | A→T | 5.2% | intergenic (‑175/‑25) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
| RA | NZ_CP022686 | 3,348,371 | T→G | 7.3% | intergenic (‑187/‑13) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
| RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
| RA | NZ_CP022686 | 3,444,704 | T→G | 12.7% | T530P (ACC→CCC) | btsT ← | pyruvate/proton symporter BtsT |
| RA | NZ_CP022686 | 3,451,845 | A→C | 9.8% | E278D (GAA→GAC) | lgoD → | L‑galactonate oxidoreductase |
| RA | NZ_CP022686 | 3,461,079 | G→C | 9.4% | intergenic (+13/+26) | yjjZ → / ← CIW80_RS17335 | DUF1435 domain‑containing protein/tRNA‑Leu |
| RA | NZ_CP022686 | 3,461,200 | C→A | 16.6% | intergenic (‑9/+26) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
| RA | NZ_CP022686 | 3,461,218 | G→C | 12.7% | intergenic (‑27/+8) | CIW80_RS17335 ← / ← CIW80_RS17340 | tRNA‑Leu/tRNA‑Leu |
| RA | NZ_CP022686 | 3,461,229 | T→G | 21.1% | noncoding (84/87 nt) | CIW80_RS17340 ← | tRNA‑Leu |
| RA | NZ_CP022686 | 3,516,075 | T→G | 7.2% | V213G (GTG→GGG) | nhaA → | Na+/H+ antiporter NhaA |
| MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | 100% | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
| RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
| RA | NZ_CP022686 | 3,713,182 | A→C | 11.8% | E39D (GAA→GAC) | yaeR → | VOC family protein |
| RA | NZ_CP022686 | 3,734,367 | T→G | 14.0% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
| RA | NZ_CP022686 | 3,787,760 | G→T | 6.9% | I70I (ATC→ATA) | CIW80_RS19000 ← | hypothetical protein |
| RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
| RA | NZ_CP022686 | 3,797,481 | T→G | 14.7% | L130V (TTA→GTA) ‡ | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
| RA | NZ_CP022686 | 3,797,483 | A→T | 13.1% | L130F (TTA→TTT) ‡ | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
| RA | NZ_CP022686 | 3,800,981 | A→C | 11.1% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
| RA | NZ_CP022686 | 3,806,265 | T→A | 11.6% | Y1586N (TAC→AAC) | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
| RA | NZ_CP022686 | 3,808,221 | A→C | 6.5% | S2238R (AGC→CGC) | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
| MC JC | NZ_CP022686 | 3,844,426 | Δ1,036 bp | 100% | coding (2‑1037/1056 nt) | dinB → | DNA polymerase IV |
| RA | NZ_CP022686 | 3,852,481 | G→T | 6.3% | Q171K (CAA→AAA) | phoE ← | phosphoporin PhoE |
| RA | NZ_CP022686 | 3,868,846 | T→G | 14.0% | T383P (ACG→CCG) | ecpC ← | fimbrial usher EcpC |
| RA | NZ_CP022686 | 3,910,255 | G→T | 7.8% | F69L (TTC→TTA) | yahB ← | LysR substrate‑binding domain‑containing protein |
| RA | NZ_CP022686 | 3,935,324 | T→G | 16.9% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
| RA | NZ_CP022686 | 3,949,969 | T→G | 12.5% | D141A (GAC→GCC) | hemB ← | porphobilinogen synthase |
| RA | NZ_CP022686 | 3,981,933 | T→G | 13.5% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
| RA | NZ_CP022686 | 3,984,841 | T→G | 12.2% | R67R (CGT→CGG) | queA → | tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA |
| RA | NZ_CP022686 | 4,014,238 | C→G | 11.8% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
| RA | NZ_CP022686 | 4,056,962 | C→A | 18.9% | F137L (TTC→TTA) | adk → | adenylate kinase |
| RA | NZ_CP022686 | 4,079,329 | A→C | 6.6% | S170A (TCA→GCA) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
| RA | NZ_CP022686 | 4,188,944 | A→C | 13.1% | V321G (GTA→GGA) | rlpA ← | endolytic peptidoglycan transglycosylase RlpA |
| RA | NZ_CP022686 | 4,287,532 | G→T | 6.6% | noncoding (45/76 nt) | CIW80_RS21480 → | tRNA‑Lys |
| RA | NZ_CP022686 | 4,301,920 | T→G | 12.6% | V136G (GTT→GGT) | modB → | molybdate ABC transporter permease subunit |
| RA | NZ_CP022686 | 4,562,550 | T→G | 14.2% | N140H (AAC→CAC) | putA ← | trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase |
| RA | NZ_CP022686 | 4,583,784 | G→T | 17.2% | R78S (AGG→AGT) | CIW80_RS22900 → | YfjI family protein |
| RA | NZ_CP022686 | 4,632,182 | T→G | 8.0% | R276R (CGT→CGG) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
| RA | NZ_CP022686 | 4,642,951 | A→C | 8.5% | N732T (AAC→ACC) | sfaF → | S/F1C fimbrial usher SfaF/FocD |
| RA | NZ_CP022686 | 4,678,041 | A→C | 21.4% | intergenic (+39/‑82) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
| RA | NZ_CP022686 | 4,678,047 | A→C | 33.1% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
| RA | NZ_CP022686 | 4,764,914 | C→A | 12.9% | intergenic (‑94/‑167) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
| RA | NZ_CP022686 | 4,809,441 | T→G | 10.7% | G50G (GGT→GGG) | CIW80_RS24260 → | head‑tail joining protein |
| MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | 100% | [umuD]–[umuC] | [umuD], [umuC] | |
| RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
| RA | NZ_CP022686 | 4,919,017 | T→G | 10.9% | E330D (GAA→GAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
| RA | NZ_CP022686 | 5,042,289 | T→C | 7.1% | N640N (AAT→AAC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,042,304 | T→C | 7.8% | N645N (AAT→AAC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 92:1 | +A | 34.3% | intergenic (‑44/‑113) | putative HTH domain protein ← / → uncharacterized protein | –/– |
| RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
| JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] | |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NZ_MW240712 | 667–2618 | 3048–2957 | 340–2382 | 7517 [6862] | [6833] 7155 | JNG15_RS00010–mobA | JNG15_RS00010,JNG15_RS00015,mobA |
| * | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4748–4810 | 5478–5213 | 404–731 | 5818 [5703] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP022686 | 262466 = | 3 (0.020) | 179 (1.030) | 74/250 | NT | 98.4% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
| ? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
| * | ? | NZ_CP022686 | = 262468 | 3 (0.020) | 134 (0.770) | 62/250 | NT | 97.8% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
| ? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase | |||||
| * | ? | NZ_CP022686 | 1522881 = | NA (NA) | 7502 (1.580) | 226/250 | NT | 71.8% | intergenic (‑394/‑72) | yqcC/CIW80_RS07590 | YqcC family protein/IS3‑like element ISEc52 family transposase |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3853 = | 2940 (0.320) | coding (245/399 nt) | IL2wt no signal | ||||||
| * | ? | NZ_CP022686 | = 1524127 | NA (NA) | 7924 (1.710) | 222/244 | NT | 73.7% | intergenic (+5/+235) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3859 | 2899 (0.310) | coding (251/399 nt) | IL2wt no signal | ||||||
| * | ? | NZ_CP022686 | 4514374 = | 179 (1.030) | 9 (0.000) +AGCT |
8/242 | NT | 9.0% | coding (59/1053 nt) | ompA | porin OmpA |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3609 = | 10 (0.000) | coding (1/399 nt) | IL2wt no signal | ||||||