breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 192,476 T→A 11.0% R629R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 211,894 A→C 9.4% K127N (AAA→AAC gloA → lactoylglutathione lyase
RA NZ_CP022686 298,027 A→C 11.9% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,100 A→C 15.2% T605P (ACG→CCG)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,106 A→C 10.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 498,662 A→C 14.1% C183G (TGT→GGT)  araH ← arabinose ABC transporter permease AraH
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,422 A→C 12.2% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 597,830 A→C 14.9% D452A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 16.9% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.8% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,757 C→A 14.0% G438W (GGG→TGG)  clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 12.4% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,435 C→A 25.2% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 22.7% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 881,733 A→C 14.6% intergenic (+248/+139) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 17.5% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 972,697 A→C 6.6% T7P (ACG→CCG)  rcsD → phosphotransferase RcsD
RA NZ_CP022686 1,065,978 A→C 10.1% F37V (TTT→GTT)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,907 T→G 12.6% E199D (GAA→GAC aroC ← chorismate synthase
RA NZ_CP022686 1,209,908 T→G 24.2% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,280,117 T→G 6.2% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,311,302 A→C 7.7% L245R (CTC→CGC)  yphD ← ABC transporter permease
RA NZ_CP022686 1,360,709 T→G 11.6% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 8.7% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,479,607 A→C 14.7% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,355 T→A 12.8% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,307 A→C 12.2% D16A (GAC→GCC)  fucI → L‑fucose isomerase
RA NZ_CP022686 1,546,986 T→A 8.1% intergenic (+8/‑26) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,566,203 A→C 10.2% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,501 T→G 11.0% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,674,224 T→G 17.6% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 15.3% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 10.5% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,698,738 T→A 9.2% Q455L (CAG→CTG)  tkt ← transketolase
RA NZ_CP022686 1,705,974 T→G 10.9% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,793,114 A→C 6.3% D219A (GAC→GCC)  CIW80_RS08935 → GTPase family protein
RA NZ_CP022686 1,796,825 T→G 13.6% intergenic (+38/‑57) agn43 → / → CIW80_RS08945 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 1,828,110 A→C 7.3% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,832,023 T→G 9.0% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,954,923 T→G 16.6% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 6.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 9.0% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 10.3% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,037,521 A→C 9.9% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,094 G→T 10.7% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 7.4% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,076,568 C→G 7.8% intergenic (+38/‑195) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 12.0% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 10.7% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 10.0% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,114 C→G 15.5% L126V (CTG→GTG) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 14.0% L126R (CTG→CGG) ‡ panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 6.8% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,185,075 A→C 9.9% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,291 A→C 11.5% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,324 A→C 16.3% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,362,972 T→G 6.3% F158V (TTT→GTT)  nikB → nickel ABC transporter permease subunit NikB
RA NZ_CP022686 2,394,287 T→G 18.1% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 11.8% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,522 T→A 9.3% V19V (GTA→GTT yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 9.9% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,873 A→C 9.1% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,572,705 A→C 15.7% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,572,721 A→C 13.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 11.7% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 6.2% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,736,585 T→A 9.5% Y183N (TAC→AAC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 10.1% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,780,127 C→A 9.2% P683T (CCA→ACA)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,811,355 C→G 7.0% R16P (CGT→CCT)  rfaH ← transcription/translation regulatory transformer protein RfaH
RA NZ_CP022686 2,871,422 T→G 9.3% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,977,481 T→G 6.1% V558G (GTA→GGA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA NZ_CP022686 2,992,831 A→C 10.4% K133T (AAA→ACA)  nfi → deoxyribonuclease V
RA NZ_CP022686 3,020,229 T→G 11.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,136,940 T→G 12.8% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 6.0% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,213,819 T→G 7.2% R479R (CGT→CGG nnr → bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase
RA NZ_CP022686 3,265,096 G→T 11.5% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,059 T→G 10.2% L93R (CTG→CGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,348,357 T→G 7.8% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,625 C→A 18.9% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 10.6% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,451,834 C→A 7.7% H275N (CAT→AAT)  lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,202 T→A 11.2% intergenic (‑11/+24) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 13.5% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 14.8% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 21.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 7.3% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
RA NZ_CP022686 3,504,054 T→G 10.0% V204G (GTT→GGT)  tal → transaldolase
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,567,599 A→C 8.4% T269P (ACC→CCC)  araC → arabinose operon transcriptional regulator AraC
RA NZ_CP022686 3,604,565 A→T 14.6% intergenic (+81/‑150) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,075 C→A 23.6% D157Y (GAC→TAC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,734,367 T→G 15.4% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 14.9% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,758,887 C→A 10.2% K205N (AAG→AAT CIW80_RS18810 ← hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,798,092 C→A 10.8% F333L (TTC→TTA CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,897,762 C→A 17.2% pseudogene (114/1083 nt) hyxB ← outer membrane autotransporter HyxB
RA NZ_CP022686 3,932,443 C→A 14.6% E184* (GAA→TAA)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 11.5% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,942,583 C→A 10.0% D112Y (GAT→TAT)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 4,014,238 C→G 9.0% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 22.8% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,024,243 T→G 10.8% T228P (ACC→CCC)  queC ← 7‑cyano‑7‑deazaguanine synthase QueC
RA NZ_CP022686 4,056,962 C→A 15.4% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,067,840 A→C 9.0% K7N (AAA→AAC ybaQ → HigA family addiction module antitoxin
RA NZ_CP022686 4,196,276 T→G 12.5% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,280,095 T→G 15.2% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,287,524 A→T 7.5% noncoding (37/76 nt) CIW80_RS21480 → tRNA‑Lys
RA NZ_CP022686 4,368,341 T→G 15.2% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,461,745 G→T 9.4% intergenic (+99/‑71) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,571,414 T→G 7.7% P67P (CCA→CCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,589,347 T→G 10.9% P253P (CCA→CCC CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 7.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,759,424 T→A 7.7% T203S (ACG→TCG)  ldtC ← L,D‑transpeptidase LdtC
RA NZ_CP022686 4,762,516 T→G 12.8% K368N (AAA→AAC mfd ← transcription‑repair coupling factor
RA NZ_CP022686 4,764,877 T→G 11.1% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,880 T→G 9.4% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 10.2% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,961 T→G 9.0% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,951,545 A→C 14.7% S47R (AGT→CGT)  topA → type I DNA topoisomerase
RA NZ_CP022686 4,980,895 A→C 13.4% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,001,151 G→C 10.0% A159P (GCC→CCC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,041,191 G→A 8.0% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,149 T→C 7.6% G1260G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,182 T→A 7.9% G1271G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,215 T→G 14.6% N1282K (AAT→AAG CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ50 bp 23.4% [IL2wt no signal] [IL2wt no signal]
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 7.5% E52* (GAA→TAA)  IL2wt no signal →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 680–2618 3047–2957 340–2368 3740 [3701] [3592] 3734 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4742–4866 5478–5213 348–737 3481 [3465] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =1 (0.010)180 (1.020) 82/248 NT 99.4% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.010)138 (0.780) 65/248 NT 99.3% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 1522881 =NA (NA)448 (0.160) 113/248 NT 17.5% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3537 2115 (0.400)coding (7/63 nt) ompA
* ? NZ_CP022686 1522881 =NA (NA)19 (0.010)
+GTAGGGG
16/234 NT 80.1% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 5 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 1522881 =NA (NA)50 (0.020) 33/248 NT 41.0% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3619 = 72 (0.010)coding (11/399 nt) IL2wt no signal
* ? NZ_CP022686 1522882 =NA (NA)283 (0.100) 87/246 NT 5.6% intergenic (‑395/‑71) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3902 4809 (0.900)coding (294/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1524129NA (NA)328 (0.120) 72/246 NT 12.4% intergenic (+7/+233) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 3534 = 2331 (0.440)coding (4/63 nt) ompA
* ? NZ_CP022686 = 1524130NA (NA)36 (0.010) 28/248 NT 87.8% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 3614 = 5 (0.000)coding (6/399 nt) IL2wt no signal
* ? NZ_CP022686 1760445 =NA (NA)15 (0.010)
+38 bp
14/172 NT 81.3% coding (1096/1101 nt) CIW80_RS08705 IS30‑like element IS30 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 5 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 1760449 =NA (NA)277 (0.100) 90/246 NT 5.5% coding (1100/1101 nt) CIW80_RS08705 IS30‑like element IS30 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3898 = 4762 (0.890)coding (290/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1761699NA (NA)7 (0.000)
+21 bp
7/206 NT 62.8% intergenic (‑74/‑68) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 5 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3369608 =NA (NA)59 (0.020)
+AGAGGTAGGGG
41/226 NT 92.8% intergenic (‑1/+10) CIW80_RS16840/CIW80_RS16845 restriction endonuclease/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 5 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3393988 =NA (NA)12 (0.010)
+CGTCGATGAAGAGGTAGGGG
11/208 NT 74.1% intergenic (‑51/+11) CIW80_RS16975/CIW80_RS16980 hypothetical protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 5 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–