breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 23,822 A→C 7.7% N124T (AAC→ACC)  ydcR → PLP‑dependent aminotransferase family protein
RA NZ_CP022686 340,516 A→C 10.0% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 349,106 A→C 11.8% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 362,802 G→T 7.5% intergenic (‑4/+78) rnd ← / ← fadD ribonuclease D/long‑chain‑fatty‑acid‑‑CoA ligase FadD
RA NZ_CP022686 372,574 A→C 11.9% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 410,020 G→T 10.8% L372M (CTG→ATG)  zwf ← glucose‑6‑phosphate dehydrogenase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,135 A→C 13.2% V382V (GTT→GTG otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 531,408 A→C 15.8% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,940 A→T 17.3% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 556,942 G→T 15.5% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 10.0% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 19.9% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 15.1% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 18.1% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,763 A→T 13.6% Y288* (TAT→TAA wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 27.3% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 25.4% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.4% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 15.7% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 859,943 T→G 10.8% L602V (TTA→GTA)  yehM → DUF5682 family protein
RA NZ_CP022686 859,954 A→C 11.1% R605R (CGA→CGC yehM → DUF5682 family protein
RA NZ_CP022686 881,739 A→C 16.9% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 19.6% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,046 G→T 21.1% intergenic (+115/‑142) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 17.4% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,150 A→C 10.5% intergenic (+219/‑38) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 12.5% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,431 T→G 30.1% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 15.1% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,426 G→C 12.8% M1M (ATG→ATC) † glpA → anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,047,897 T→G 7.5% intergenic (‑127/+37) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,120,330 A→C 5.5% V2556G (GTC→GGC)  CIW80_RS05650 ← phase‑variable autotransporter adhesin UpaE
RA NZ_CP022686 1,174,272 A→C 11.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,269,551 T→G 8.7% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,276,970 A→C 6.5% G414G (GGT→GGG der ← ribosome biogenesis GTPase Der
RA NZ_CP022686 1,278,807 T→G 8.7% Q234P (CAG→CCG)  bamB ← outer membrane protein assembly factor BamB
RA NZ_CP022686 1,360,709 T→G 12.3% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 9.3% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,479,607 A→C 10.6% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,310 G→T 14.1% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,564,569 A→C 14.0% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,573 T→G 10.8% W192G (TGG→GGG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,217 A→C 21.4% T740P (ACG→CCG)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,901 T→G 8.9% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 14.0% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 11.6% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 17.2% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,273 C→G 9.6% C172S (TGC→TCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,047 G→T 11.0% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,710,453 A→C 7.8% intergenic (+48/‑32) endA → / → rsmE deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA NZ_CP022686 1,716,202 A→C 11.4% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,832,023 T→G 12.5% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,832,028 A→T 10.0% H353Q (CAT→CAA gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,889,761 A→C 7.9% V459G (GTT→GGT)  ygiQ ← YgiQ family radical SAM protein
RA NZ_CP022686 1,954,923 T→G 21.5% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,037,521 A→C 8.3% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 13.8% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,061,804 A→C 11.4% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,127,718 T→G 11.0% D34A (GAC→GCC)  yhdP ← AsmA2 domain‑containing protein YhdP
RA NZ_CP022686 2,148,115 T→G 20.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 15.4% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,163,522 C→A 5.3% intergenic (‑17/+23) rrf ← / ← CIW80_RS10805 5S ribosomal RNA/tRNA‑Thr
RA NZ_CP022686 2,172,177 A→C 6.0% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,063 T→A 7.1% Y50F (TAC→TTC)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,185,075 A→C 11.5% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,250,445 G→C 6.4% L43V (CTC→GTC)  dam ← adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,361,493 A→T 9.7% L189F (TTA→TTT nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,394,287 T→G 16.3% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,302 A→T 14.1% F110Y (TTC→TAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,579 T→A 26.8% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,460,581 A→C 29.7% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,533,251 T→A 8.3% Y296F (TAC→TTC)  tdh ← L‑threonine 3‑dehydrogenase
RA NZ_CP022686 2,558,864 T→G 7.7% F128V (TTT→GTT)  yicG → trimeric intracellular cation channel family protein
RA NZ_CP022686 2,574,429 T→G 14.8% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,736,576 A→C 5.6% T180P (ACC→CCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 12.8% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 15.7% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,868,740 T→G 26.6% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 10.6% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 3,020,229 T→G 14.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,090,101 A→C 11.3% V9G (GTT→GGT)  yjcB ← YjcB family protein
RA NZ_CP022686 3,136,948 G→T 9.8% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 19.0% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,348,371 T→G 7.5% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,785 T→G 5.9% T503P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,202 T→A 11.6% intergenic (‑11/+24) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,215 A→T 14.0% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 17.6% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,233 A→T 12.7% noncoding (80/87 nt) CIW80_RS17340 ← tRNA‑Leu
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,570,234 T→G 8.7% L217V (TTG→GTG)  yabI → DedA family protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,770,508 T→G 11.4% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,775,617 A→C 5.3% T6P (ACG→CCG)  CIW80_RS18935 → putative zinc ribbon protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 11.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 14.7% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,852,459 T→G 9.3% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,928,345 T→G 9.6% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 12.4% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 14.6% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,979,381 T→G 8.6% L96R (CTT→CGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 3,988,205 T→G 5.9% G310G (GGT→GGG secD → protein translocase subunit SecD
RA NZ_CP022686 4,056,951 C→A 17.3% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 18.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,214,324 T→A 9.8% noncoding (14/77 nt) CIW80_RS21090 ← tRNA‑Met
RA NZ_CP022686 4,290,599 T→G 9.8% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,439,653 A→C 9.0% K570N (AAA→AAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,535,285 A→C 8.7% K97T (AAG→ACG)  appA → bifunctional acid phosphatase/4‑phytase
RA NZ_CP022686 4,571,417 T→G 13.2% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,605,579 T→G 12.7% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,186 G→T 9.5% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,037 C→A 17.0% intergenic (+35/‑86) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,041 A→C 15.9% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,680,205 T→G 6.4% S695A (TCA→GCA)  CIW80_RS23450 → hypothetical protein
RA NZ_CP022686 4,764,877 T→G 9.8% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,814,990 T→G 10.9% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,916,128 A→C 8.4% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,919,022 T→G 12.3% S329R (AGC→CGC)  adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,980,895 A→C 9.3% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,041,191 G→A 5.6% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.3% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 11.9% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 370–2618 3067–2957 340–2698 5861 [5762] [5749] 5831 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4748–4809 5416–5214 406–669 3673 [3533] [3651] 3684 [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =3 (0.020)194 (1.150) 60/250 NT 98.5% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624683 (0.020)158 (0.930) 53/250 NT 98.1% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase