breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 11.8% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 192,476 T→A 8.7% R629R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 196,840 A→C 6.4% N132T (AAC→ACC)  dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 235,846 A→C 8.4% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 238,324 G→C 18.0% V85V (GTC→GTG sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 269,554 A→C 9.2% G269G (GGT→GGG selO ← protein adenylyltransferase SelO
RA NZ_CP022686 311,464 A→C 10.2% E119D (GAA→GAC ydjY → lipoprotein YdjY
RA NZ_CP022686 340,516 A→C 14.5% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 350,056 A→C 18.3% K241T (AAA→ACA)  yeaH → YeaH/YhbH family protein
RA NZ_CP022686 359,909 C→A 11.2% D20Y (GAT→TAT)  yoaG ← DUF1869 domain‑containing protein
RA NZ_CP022686 359,976 C→A 7.8% G118G (GGG→GGT yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,227 T→G 17.5% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,236 C→A 14.3% D32Y (GAT→TAT)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,256 C→A 21.4% G25V (GGA→GTA)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 14.5% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,942 G→T 13.2% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 585,422 A→C 12.1% V585G (GTG→GGG)  ybtQ ← yersiniabactin ABC transporter ATP‑binding/permease protein YbtQ
RA NZ_CP022686 601,295 T→G 22.2% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 22.4% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 631,166 A→C 6.9% L357V (TTA→GTA)  clbO ← colibactin polyketide synthase ClbO
RA NZ_CP022686 651,848 A→C 11.3% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 693,001 A→C 6.0% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 766,757 T→G 21.1% K290N (AAA→AAC wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 777,337 A→C 9.5% R225R (CGT→CGG gmd ← GDP‑mannose 4,6‑dehydratase
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 27.4% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 17.0% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,472 G→T 17.6% intergenic (‑61/+166) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,486 C→A 9.6% intergenic (‑75/+152) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 878,917 A→C 6.3% intergenic (+109/+11) yohD → / ← yohF DedA family protein/SDR family oxidoreductase
RA NZ_CP022686 896,395 T→G 10.4% W17G (TGG→GGG)  yeiG → S‑formylglutathione hydrolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 954,525 A→C 12.5% S38A (TCT→GCT)  ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA NZ_CP022686 957,112 T→G 13.3% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 18.1% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,890 T→G 17.8% V226G (GTC→GGC)  rbn → ribonuclease BN
RA NZ_CP022686 1,040,909 A→C 10.7% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,047,906 G→T 8.4% intergenic (‑136/+28) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
JC NZ_CP022686 1,061,329 Δ6 bp 10.6% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,065,997 A→C 12.3% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,112,973 A→C 7.6% V127G (GTG→GGG)  fadJ ← fatty acid oxidation complex subunit alpha FadJ
RA NZ_CP022686 1,174,272 A→C 8.7% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,209,908 T→G 21.9% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,209,917 G→T 13.2% P393Q (CCA→CAA)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 10.1% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,276,970 A→C 6.8% G414G (GGT→GGG der ← ribosome biogenesis GTPase Der
RA NZ_CP022686 1,280,117 T→G 6.2% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,319,611 C→A 16.2% D325Y (GAT→TAT)  glyA ← serine hydroxymethyltransferase
RA NZ_CP022686 1,325,422 G→T 6.9% Q252K (CAA→AAA)  qseE ← two component system sensor histidine kinase QseE/GlrK
RA NZ_CP022686 1,358,659 C→A 6.4% Y338* (TAC→TAA pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA NZ_CP022686 1,360,709 T→G 8.6% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 13.4% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 7.0% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 12.2% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 11.3% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,762 G→T 9.8% intergenic (‑97/+227) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,277 T→G 11.4% intergenic (‑278/+164) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,371,081 C→A 13.6% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,392,487 A→C 7.9% T43P (ACG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase
RA NZ_CP022686 1,441,800 T→G 5.2% N587T (AAC→ACC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,447,152 G→T 8.2% R51S (CGC→AGC)  hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,479,585 T→A 8.6% K3* (AAA→TAA)  ftsB ← cell division protein FtsB
RA NZ_CP022686 1,479,607 A→C 12.1% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,355 T→A 12.2% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 16.5% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,365 A→C 22.4% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,546,985 A→T 8.5% intergenic (+7/‑27) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,003 G→C 11.5% intergenic (+25/‑9) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 18.7% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,372 A→C 6.4% R457R (AGG→CGG)  vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,558,395 A→C 9.6% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 10.5% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,579,306 A→C 6.1% G109G (GGT→GGG CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,632,501 T→G 8.0% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 27.8% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 10.0% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,231 A→T 11.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 9.5% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 11.2% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,758,771 T→G 7.2% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,805,023 C→A 9.1% pseudogene (304/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,833,745 T→G 18.2% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,833,762 A→C 12.9% L462R (CTG→CGG)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,911,587 C→A 6.5% R229L (CGT→CTT)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 9.8% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,925,365 A→C 5.6% D162A (GAC→GCC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,979,438 T→G 7.5% F531V (TTT→GTT)  ygjK → alpha‑glucosidase
RA NZ_CP022686 2,037,515 G→T 9.1% E506* (GAA→TAA)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,037,521 A→C 8.7% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,049,089 T→G 18.9% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 12.7% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,143 A→C 5.2% S623A (TCC→GCC)  infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,056,168 A→C 8.2% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,780 A→C 9.3% K383N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,072,535 G→T 6.3% H357N (CAC→AAC)  cgtA ← Obg family GTPase CgtA
RA NZ_CP022686 2,076,552 A→C 7.6% intergenic (+22/‑211) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,554 T→G 14.3% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,556 C→G 5.7% intergenic (+26/‑207) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,090,800 T→G 7.2% S166A (TCG→GCG)  yrbL → PhoP regulatory network protein YrbL
RA NZ_CP022686 2,110,457 T→G 12.8% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.2% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,481 A→T 6.4% F4L (TTT→TTA rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 9.2% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,109 T→G 12.5% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 15.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 8.6% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 14.4% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,160 T→G 6.3% V141G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 5.6% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,075 A→C 10.5% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,218 A→C 6.8% intergenic (‑87/+78) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,194,054 G→T 7.1% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,207,086 T→A 6.5% intergenic (‑110/+59) bfd ← / ← chiA bacterioferritin‑associated ferredoxin/bifunctional chitinase/lysozyme
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,222,564 A→C 7.9% T428P (ACG→CCG)  yheS → ABC transporter ATP‑binding protein
RA NZ_CP022686 2,226,139 A→C 6.1% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,310,451 C→A 9.7% E241* (GAA→TAA)  glgP ← glycogen phosphorylase
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,324 A→C 14.9% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,380,922 A→C 8.7% S182R (AGC→CGC)  pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,380,936 A→C 7.9% K186N (AAA→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,386,496 T→G 6.1% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,394,287 T→G 14.1% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,579 T→A 20.0% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 11.4% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,546,394 A→C 13.2% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 10.4% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,305 T→G 5.4% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
RA NZ_CP022686 2,572,721 A→C 9.6% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 12.3% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,467 C→G 7.0% Q217H (CAG→CAC CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 7.7% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,717,400 T→G 6.9% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,733,571 T→G 10.4% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,586 A→C 14.7% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 14.1% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,113 A→C 5.7% A35A (GCA→GCC yifL → lipoprotein
RA NZ_CP022686 2,758,146 G→C 5.8% T46T (ACG→ACC yifL → lipoprotein
RA NZ_CP022686 2,801,827 C→A 8.8% E29* (GAA→TAA)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,811,073 G→T 6.8% P110Q (CCG→CAG)  rfaH ← transcription/translation regulatory transformer protein RfaH
RA NZ_CP022686 2,834,869 G→T 9.2% E592* (GAA→TAA)  polA → DNA polymerase I
RA NZ_CP022686 2,834,886 A→C 13.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,834,892 G→C 6.1% L599L (CTG→CTC polA → DNA polymerase I
RA NZ_CP022686 2,834,898 A→C 9.7% K601N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,849,863 G→T 7.3% I174I (ATC→ATA CIW80_RS14255 ← DeoR/GlpR family DNA‑binding transcription regulator
RA NZ_CP022686 2,868,740 T→G 23.6% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 16.5% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,920,418 C→A 6.0% A65E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,937,268 G→T 5.9% P199Q (CCA→CAA)  yijO ← AraC family transcriptional regulator
RA NZ_CP022686 2,989,627 T→G 7.2% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 2,994,693 T→G 8.8% W162G (TGG→GGG)  yjaH → DUF1481 domain‑containing protein
RA NZ_CP022686 3,098,847 A→C 8.6% intergenic (+17/+22) CIW80_RS15415 → / ← actP LrgB family protein/cation/acetate symporter ActP
RA NZ_CP022686 3,163,954 A→C 6.8% T400P (ACC→CCC)  melA → alpha‑galactosidase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,096 G→T 11.4% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,287,046 A→C 7.2% K117N (AAA→AAC cybC → cytochrome b562
RA NZ_CP022686 3,297,360 T→G 10.0% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,371 T→G 6.3% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,386 C→A 5.0% M1M (ATC→ATA) † CIW80_RS26495 → hypothetical protein
RA NZ_CP022686 3,357,439 G→T 7.7% G8C (GGT→TGT)  CIW80_RS16765 → DUF932 domain‑containing protein
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 14.5% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,459,370 C→A 11.1% S62Y (TCT→TAT)  bglJ → DNA‑binding transcriptional activator BglJ
RA NZ_CP022686 3,461,215 A→T 11.2% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,226 T→G 12.0% noncoding (87/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 22.8% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 14.8% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,469,923 T→G 7.2% E122D (GAA→GAC yjjW ← YjjW family glycine radical enzyme activase
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 22.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,606,036 G→T 10.2% E250* (GAA→TAA)  secA → preprotein translocase subunit SecA
RA NZ_CP022686 3,625,683 T→G 8.9% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,650,128 T→G 9.9% V25G (GTT→GGT)  CIW80_RS18240 → hypothetical protein
RA NZ_CP022686 3,652,079 T→G 8.6% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 8.0% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,536 G→C 7.0% A766G (GCT→GGT)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,075 C→A 5.8% D157Y (GAC→TAC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,680,536 A→C 5.9% V45G (GTT→GGT)  btuF ← vitamin B12 ABC transporter substrate‑binding protein BtuF
RA NZ_CP022686 3,703,648 A→C 8.2% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,710,986 T→G 5.2% V40G (GTT→GGT)  ldcC → lysine decarboxylase LdcC
RA NZ_CP022686 3,734,367 T→G 17.0% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,372 C→G 10.6% G226G (GGC→GGG yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,736,531 T→G 11.2% N74H (AAC→CAC)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,770,508 T→G 8.9% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 7.3% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 12.2% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,806,265 T→A 8.3% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,846 T→G 10.2% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,457 A→C 11.7% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,897,759 A→T 10.2% pseudogene (117/1083 nt) hyxB ← outer membrane autotransporter HyxB
RA NZ_CP022686 3,925,289 A→C 9.2% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,345 T→G 6.6% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,356 T→G 5.3% A280A (GCT→GCG prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,940,707 A→C 9.3% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 10.5% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 9.1% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,977,656 A→C 7.7% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,979,408 T→G 7.6% V105G (GTT→GGT)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ
RA NZ_CP022686 3,981,933 T→G 14.0% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,004,135 T→G 12.1% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,225 C→G 8.1% intergenic (+71/‑273) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,238 C→G 13.4% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 19.2% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,257 G→T 13.1% E95* (GAA→TAA)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,032,292 A→C 12.4% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 25.5% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,329 A→C 11.1% S170A (TCA→GCA)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,213,260 T→G 7.3% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,286,467 T→G 6.7% V42G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,290,635 T→G 6.6% T109P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,331,929 C→A 13.6% M292I (ATG→ATT ybhR ← ABC transporter permease
RA NZ_CP022686 4,331,940 A→C 16.5% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,338,890 A→C 5.3% Q441H (CAA→CAC rhlE → ATP‑dependent RNA helicase RhlE
RA NZ_CP022686 4,439,653 A→C 7.0% K570N (AAA→AAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,328 A→T 10.1% Q795H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,461,745 G→T 5.9% intergenic (+99/‑71) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL
RA NZ_CP022686 4,485,614 T→G 10.1% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,605,579 T→G 12.9% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein
RA NZ_CP022686 4,632,182 T→G 10.4% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,661,459 T→G 6.9% pseudogene (559/655 nt) CIW80_RS26570 → hypothetical protein
RA NZ_CP022686 4,678,047 A→C 22.7% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 16.7% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,877 T→G 14.2% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,783,334 A→C 13.5% V32G (GTC→GGC)  mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,787,799 C→A 8.3% intergenic (‑52/‑44) CIW80_RS24075 ← / → CIW80_RS24080 IS3‑like element ISEc52 family transposase/super‑infection exclusion protein B
RA NZ_CP022686 4,839,668 T→G 16.1% V150V (GTA→GTC minD ← septum site‑determining protein MinD
RA NZ_CP022686 4,839,687 T→G 7.6% N144T (AAC→ACC)  minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 5,001,125 A→C 12.3% N150T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,001,157 G→T 15.2% D161Y (GAT→TAT)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,043,558 T→C 8.3% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 5.5% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→C 7.2% G1162G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ50 bp 13.4% [IL2wt no signal] [IL2wt no signal]
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
RA pMut‑Ompa‑D5‑IL2wt‑His6 3,762 G→T 57.6% E52* (GAA→TAA)  IL2wt no signal →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 395–2618 3049–2957 340–2655 8361 [8348] [8220] 8451 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4744–4810 5417–5214 405–674 7059 [7003] [6662] 7517 [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =2 (0.010)218 (0.960) 63/250 NT 99.1% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624682 (0.010)222 (0.980) 67/250 NT 99.1% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 1522881 =NA (NA)2195 (0.400) 150/250 NT 38.3% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3537 3530 (0.320)coding (7/63 nt) ompA
* ? NZ_CP022686 1522881 =NA (NA)144 (0.030)
+GTAGGGG
54/236 NT 92.1% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 1522881 =NA (NA)139 (0.030) 48/250 NT 47.4% intergenic (‑394/‑72) yqcC/CIW80_RS07590 YqcC family protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3619 = 154 (0.010)coding (11/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1524129NA (NA)1655 (0.300) 93/248 NT 29.3% intergenic (+7/+233) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 3534 = 4019 (0.370)coding (4/63 nt) ompA
* ? NZ_CP022686 = 1524130NA (NA)132 (0.020) 55/250 NT 91.0% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 3614 = 13 (0.000)coding (6/399 nt) IL2wt no signal
* ? NZ_CP022686 1760445 =NA (NA)14 (0.000)
+38 bp
11/174 NT 60.7% coding (1096/1101 nt) CIW80_RS08705 IS30‑like element IS30 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 = 1761699NA (NA)18 (0.000)
+21 bp
17/208 NT 62.5% intergenic (‑74/‑68) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 = 2700946NA (NA)14 (0.000)
+40 bp
11/170 NT 61.3% intergenic (+9/+8) CIW80_RS13525/mdtD IS3‑like element ISEc52 family transposase/multidrug transporter subunit MdtD
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3369608 =NA (NA)131 (0.030)
+AGAGGTAGGGG
49/228 NT 91.7% intergenic (‑1/+10) CIW80_RS16840/CIW80_RS16845 restriction endonuclease/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3393988 =NA (NA)22 (0.000)
+CGTCGATGAAGAGGTAGGGG
14/210 NT 66.8% intergenic (‑51/+11) CIW80_RS16975/CIW80_RS16980 hypothetical protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 13 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3393989 =NA (NA)13 (0.000)
+28 bp
10/194 NT 56.3% intergenic (‑52/+10) CIW80_RS16975/CIW80_RS16980 hypothetical protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 13 (0.000)coding (1/399 nt) IL2wt no signal
* ? NZ_CP022686 3899666 =248 (1.100)19 (0.100) 17/218 NT 7.9% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 228 (1.160)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681240 (1.060)22 (0.110) 14/218 NT 9.1% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 228 (1.160)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 4514374 =245 (1.080)7 (0.000)
+AGCT
7/242 NT 5.3% coding (59/1053 nt) ompA porin OmpA
?pMut‑Ompa‑D5‑IL2wt‑His6 3609 = 13 (0.000)coding (1/399 nt) IL2wt no signal