BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,572,721	A→C	9.0%	F186C (TTT→TGT)	CIW80_RS12885 ←	ROK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,572,721	0	A	C	9.0%	374.4 / 4.2	188	F186C (TTT→TGT)	CIW80_RS12885	ROK family protein
Reads supporting (aligned to +/- strand): ref base A (93/73); new base C (2/20); total (95/93)
Fisher's exact test for biased strand distribution p-value = 2.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCTTGATG  >  NZ_CP022686/2572586‑2572858

aGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTGGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGGCATCTGTTGATAGCGTTCACTAAAACCTCTGCCGGAAACAAACgc                                                                                                                                       >  8:854867/1‑139 (MQ=255)

  gttttGGCTAAAACGTGCCACCTGCTGCATGGCAAAAGCATCACCACGTTGTGCCTGGGTGACAATCGCCGCATAAGTGAGAAAGTTACCAGTCATCTGTTGATAGCGTTCAATAAAACCGCTGCCGGAAACAAACGa                                                                                                                                       <  3:64796/135‑1 (MQ=255)

  gtGTTGGCTAAAACGTGCCACCTGCTGCATGGCAAAAGCATCACCACGTGGTGCCAGGGTGACAAGCGCCGCAGAAGTGATCAAGTTACCAGTCATCTGTTTATAGCGTTCAATAAAACCGCTGCCGGAAACAAACGACt                                                                                                                                     <  8:782726/139‑1 (MQ=255)

  cTGTTGGCGAAAACGTGCCACCTTCTGCATGGCACAAGCATCACCACGTTGTGCAAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATATCGTAAACTACACCCGCTGCCGGAAACAAACGa                                                                                                                                       >  6:288368/1‑138 (MQ=255)

     gtGGCTAAACCGGGCCCCCGGCTGCTTGGCAAATGCAGCCCCACTTTGTGCCTGGGTGACATTCGCCGCCTAATTAAGCAAGTTACTTCTCCCCTGTTGATAGCGTTCACTAAACCCGCGGCCGGAAACACACGACTc                                                                                                                                    <  4:894211/137‑1 (MQ=255)

      tGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTcc                                                                                                                                   >  4:50446/1‑138 (MQ=255)

       ggCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCCCGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACg                                                                                                                                <  8:91904/140‑1 (MQ=255)

         cGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCAAGTATTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACg                                                                                                                                >  5:849076/1‑138 (MQ=255)

             aaCGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAACCAAACGACTCCCCTCAGTtg                                                                                                                          >  7:218787/1‑140 (MQ=255)

               cGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTtgag                                                                                                                        >  8:362371/1‑138 (MQ=255)

                         gctgcATGGCACAAGCATCACCACGTTGGGCAAGGGTTACAATCGCCGCAGAAGTGCCCAAGTTACCAGTCATCTGTTGATAGGGGTAACTAAAACCGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGc                                                                                                                <  2:214830/138‑1 (MQ=255)

                            gcATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAAt                                                                                                             >  3:132656/1‑138 (MQ=255)

                            gcATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAAt                                                                                                             >  2:758289/1‑138 (MQ=255)

                            gcATGGCACAAGCATCACCACGTGGTGCCTGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTGGATAGCGTTCACTAACACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAAt                                                                                                             <  4:128392/138‑1 (MQ=255)

                               tGGCACCAGCATCACCACTTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTCTTGATAGAGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGc                                                                                                          >  5:77104/1‑138 (MQ=255)

                                 gCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCaa                                                                                                        <  3:50446/138‑1 (MQ=255)

                                 gCACAAGAATAACCACGTTGTGCCAGGGTTACAATCGCCGCATATGTGACCAAGTAACCAGTCATGTGTTTATAGCTTTCACTAAAACCGCTGCCGTAAAAACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCaa                                                                                                        <  1:705952/138‑1 (MQ=255)

                                 ccacaAGAATCACAGGGTTGTGCGTGGGTGACAATCGCCGCAGAAGTGAGAATGTTACCTTTCATCTGTTTATTGCGTTCACTAAACCCGCTGCCTTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                                      <  8:402326/139‑1 (MQ=255)

                                   acaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACTACTCCACGCAGTTGTGTTTGCAGAAATAGCAact                                                                                                      >  4:238640/1‑138 (MQ=255)

                                   acaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                                      >  5:292162/1‑138 (MQ=255)

                                         aTCACCAGTTTGTGCCTGGGGCAAATTCGCCGCAGAATTGAGCAAGTTACCAGTAAACTGTTGATAGCGTACAATAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAActgactga                                                                                                <  2:929566/138‑1 (MQ=255)

                                         aTCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAACCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAActgactga                                                                                                >  5:233971/1‑138 (MQ=255)

                                          tCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATgg                                                                                             >  7:784678/1‑140 (MQ=255)

                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATgg                                                                                             >  1:653540/1‑138 (MQ=255)

                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           <  7:736571/140‑1 (MQ=255)

                                              cccGTTGTGCCTGGGTGAAAATCGCCGCAGAAGTGAGAATGTTACCAGTCATCTGTTTATAGGGTTCACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           <  6:301047/136‑1 (MQ=255)

                                              cacGTTGTGCCTGGGTGACAATCGCCGCAGATGTGAGCATGTTACCAGTCATCTGTTGATAGCTTTCAATAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           <  4:132656/138‑1 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGCCTCCACGCAGTTGTGTTTGCCGCAAGAGCAACTGACTGATGGAc                                                                                           >  2:95175/1‑138 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           >  4:905678/1‑138 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           >  2:673127/1‑138 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           >  6:622278/1‑138 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           >  1:256474/1‑138 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAAATTACTGATggcc                                                                                           >  1:96087/1‑136 (MQ=255)

                                              cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCGGCCGGAAACAAACGCCTACACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           >  3:212462/1‑138 (MQ=255)

                                              cacGTTCTGACAGGGTGACAATAGACGCAGAAGTGAGCAAGTTACCAGTCAACTGATGATAGAGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGaa                                                                                           >  4:905675/1‑137 (MQ=255)

                                              cacGTGGTGCCTGGGTGACAAGCGCCGCAGAAGTGCCAAAGTTACCAGTCATCTGTTGATAGCGTTCAATAAAACCGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           <  4:770959/138‑1 (MQ=255)

                                                   tgtgCCTGGGTGACAATCGCCGCAGAAGTGAGCAAGTTCCCAGTCAGCTGTTGATAGCGTTCAATAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    <  8:674857/140‑1 (MQ=255)

                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    >  7:873933/1‑140 (MQ=255)

                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    >  8:230097/1‑140 (MQ=255)

                                                   ggggCCTGGGTTACAATCGCCGCAGATGTGAGCAAGTTACCCGTCATATGTTGATAGCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                                      <  4:197468/135‑1 (MQ=255)

                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    >  1:752432/1‑138 (MQ=255)

                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    >  1:608897/1‑138 (MQ=255)

                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACGGATGGACCATCTTc                                                                                    >  4:849061/1‑138 (MQ=255)

                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATATGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGCCTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGCCTGATGGACCATCTTc                                                                                    >  5:824545/1‑138 (MQ=255)

                                                      gCCTGGGTGACAATCGCCGCATAGGTTTGCAATTTACCTGTAATCTGTTGATCGCTTTCAAAAAAACCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTcc                                                                                   <  3:355195/138‑1 (MQ=255)

                                                      gCCTGGGTGACAATCGCCGCACAAGTGAGCAAGTACCCAGGCAGCTGTTTATAGCGTTCACAAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  8:435662/140‑1 (MQ=255)

                                                       cctgggTGACAATCGCCGCTCAAGTCAGCAAGTTACCCGTCATCTGTTGATCGCGTTCACTAAACCCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                                  <  1:775831/135‑1 (MQ=255)

                                                        ctgggTGACAATCGCCGCAGAAGTGAGCATGTTACCAGGCCTCTGTTGATTGCGTGCACTAAACCCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  2:52262/136‑1 (MQ=255)

                                                        cAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATATGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  4:809857/138‑1 (MQ=255)

                                                        cAGGGTGACAATCGCCCCATAAGTGAGCAAGTTACCAGACATCTGTTGATAGCGTACAATAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  5:611306/138‑1 (MQ=255)

                                                        cAGGGGGACAATCGCCGCATAATGGAGCAAGTAACCAGTCATCTGTTGATAGCGTTCAATAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  5:910117/138‑1 (MQ=255)

                                                        cAGGGGGACAAGCGCCGCAGAAGGGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCGGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 <  6:232591/138‑1 (MQ=255)

                                                           ggTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTTATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTc                                                                              <  6:534386/138‑1 (MQ=255)

                                                             tGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            >  2:828662/1‑138 (MQ=255)

                                                             tGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            >  5:56083/1‑138 (MQ=255)

                                                             tGACAATCGCCGCAGAAGTCACCATGTTACCAGTCATCTGTTCATAGCGTTCACAAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            <  1:191826/138‑1 (MQ=255)

                                                                 aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                        >  3:75432/1‑138 (MQ=255)

                                                                      ccgcAGAAGTGAGCACGTTACCAGTCATCTGTTGATAGCGATCACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTg                                                                   <  2:68635/138‑1 (MQ=255)

                                                                        gcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                                 >  1:837495/1‑138 (MQ=255)

                                                                         cAGAAGGGAGCAAGTTACCCTGCATCTGTTGATCGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCa                                                                <  2:774026/138‑1 (MQ=255)

                                                                                  gctaGTTACCAGTCATCTTTTCATTGCGTGCACTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCTACGTGatat                                                       <  6:533712/135‑1 (MQ=255)

                                                                                   cAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACCACCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGCCTGATGGCCCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                                      >  1:469461/1‑138 (MQ=255)

                                                                                   cAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                                      >  1:377122/1‑138 (MQ=255)

                                                                                         aCCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCAtt                                              >  8:141181/1‑140 (MQ=255)

                                                                                           cAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCAtt                                              >  6:782169/1‑138 (MQ=255)

                                                                                             gcaaTCTGTTGATCCCGTTCACTAAAACCGTTCCCGGAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGAGGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCg                                            <  2:865532/135‑1 (MQ=255)

                                                                                             gTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                           <  6:234151/91‑1 (MQ=255)

                                                                                                  cTGTTTCTAGCGTTCACTAAAACCGCTGCCGGACACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTAGgcagc                                       <  2:388245/138‑1 (MQ=255)

                                                                                                  cTGTTGATAGCGTGCACTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagc                                       <  6:813432/138‑1 (MQ=255)

                                                                                                       gATAGCGTTAACTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAAATGACTGATGGACCATCTTCCTGATCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTc                                  >  6:581959/1‑138 (MQ=255)

                                                                                                          aGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg                             >  8:827065/1‑140 (MQ=255)

                                                                                                           gcgtgaaCTAAAACCGCTGCGGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGTCCGGATTCGGCAGCATTTCCCCa                              <  3:938190/132‑1 (MQ=255)

                                                                                                           gCGTTCACTAAACACGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                            <  8:148800/140‑1 (MQ=255)

                                                                                                           gCGTTCACTAAAACCCCGGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                            <  7:24760/140‑1 (MQ=255)

                                                                                                           gCGTTAACTAAAACCGCGCCCGTAAACAAACTACTCCACGCAGTTGTGTTTGACGCAATAGCAACTGACTGATGTCACATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATTACCGCATTCGGCAGCATTTCCCCAgg                            <  8:546167/140‑1 (MQ=255)

                                                                                                           ccGTTCCCTAAACCCGTCGCCGTAAACCCACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCa                              <  5:23447/137‑1 (MQ=255)

                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGCCTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATCGCCTGGCAAGGTGATATGACCGCATTCGGCAGCATTTCCCAAg                             >  4:328411/1‑138 (MQ=255)

                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGATCCGTATAGCCTGGCAACGTGATATGCCCGCATTCGGCAGCATTTCCCCAGGc                           >  8:584451/1‑140 (MQ=255)

                                                                                                             gTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                            <  2:256474/138‑1 (MQ=255)

                                                                                                              ttCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGc                           >  6:337627/1‑138 (MQ=255)

                                                                                                              ttCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGc                           <  2:608897/138‑1 (MQ=255)

                                                                                                              ttCACTAAAACCGATGCAGTAAACAAACGACTCAAAGCAGTTGTTTTTGCAGCAATAGCAACTGACTGATGGACCATCTTCCTGCTAAGTATAGCCTTTCAACGTTATATGAACGCATTCTTCAGCATTTCCCCAgtc                           <  1:153279/138‑3 (MQ=255)

                                                                                                               tCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                    >  7:808975/1‑80 (MQ=255)

                                                                                                               tCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGAATGATGGACCATCTTCCTGCTCCGTATAGCATGGCTCCGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                        >  7:361981/1‑140 (MQ=255)

                                                                                                               tCACTAAAACCGCTGCAGGAAACAAACGACTCAAAGCAGTTGTGTATGCCGCAATAGCAACTGACTGAAGGACCATCTTCCAGATACGTATAGACAGGCAAAGTGATATGACAGCAATCGGCAGCATATCACCAGGcc                          >  4:557538/1‑138 (MQ=255)

                                                                                                                 aCTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                        >  4:162667/1‑138 (MQ=255)

                                                                                                                 aCTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                        >  2:697629/1‑138 (MQ=255)

                                                                                                                 aCTAAAACAGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTACTGCTCCGTATAGCCTGGCAACGTGATATAACCGCATTCGGCAGCATTTCCCCAGGcccc                        >  2:937252/1‑138 (MQ=255)

                                                                                                                           cTGCCGGAAACACACGACTACACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCCGGCCCCTATAAACGGt              <  6:89994/138‑1 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                                 >  3:562056/1‑69 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            >  5:420781/1‑74 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            >  4:622460/1‑74 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                            <  6:420781/74‑1 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg            >  6:372863/1‑138 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg            >  3:890015/1‑138 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg            >  6:128842/1‑138 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg            >  6:546274/1‑138 (MQ=255)

                                                                                                                             gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTACTGCTCCGTATAGCCTGGCAACGTGATATGCCCGCCTTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg            >  5:3571/1‑138 (MQ=255)

                                                                                                                                  aaacaaacGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCt       <  5:502237/138‑1 (MQ=255)

                                                                                                                                       aacGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCTTGATg  <  3:162667/138‑1 (MQ=255)

AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCTTGATG  >  NZ_CP022686/2572586‑2572858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`