mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP022686 | 56,326 | C→A | 11.1% | E150* (GAA→TAA) | narH ← | nitrate reductase subunit beta |
| RA | NZ_CP022686 | 119,184 | T→G | 9.8% | F47V (TTT→GTT) | marB → | multiple antibiotic resistance protein MarB |
| RA | NZ_CP022686 | 177,514 | T→G | 6.1% | T138P (ACC→CCC) | uidB ← | glucuronide transporter |
| RA | NZ_CP022686 | 238,323 | A→C | 16.8% | F86V (TTT→GTT) | sufE ← | cysteine desulfuration protein SufE |
| RA | NZ_CP022686 | 294,947 | G→T | 5.6% | R183S (CGC→AGC) | celF ← | 6‑phospho‑beta‑glucosidase |
| RA | NZ_CP022686 | 294,971 | G→T | 6.9% | P175T (CCG→ACG) | celF ← | 6‑phospho‑beta‑glucosidase |
| RA | NZ_CP022686 | 340,478 | G→C | 6.9% | G43G (GGC→GGG) | ydjK ← | MFS transporter |
| RA | NZ_CP022686 | 340,516 | A→C | 9.8% | F31V (TTT→GTT) | ydjK ← | MFS transporter |
| RA | NZ_CP022686 | 349,106 | A→C | 14.8% | T607P (ACC→CCC) | yeaG → | protein kinase YeaG |
| RA | NZ_CP022686 | 350,040 | T→A | 9.3% | Y236N (TAC→AAC) | yeaH → | YeaH/YhbH family protein |
| RA | NZ_CP022686 | 350,052 | G→T | 14.5% | E240* (GAA→TAA) | yeaH → | YeaH/YhbH family protein |
| RA | NZ_CP022686 | 360,227 | T→G | 14.7% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
| RA | NZ_CP022686 | 364,754 | C→A | 9.7% | intergenic (‑189/+16) | fadD ← / ← yeaY | long‑chain‑fatty‑acid‑‑CoA ligase FadD/Slp family lipoprotein YeaY |
| RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
| RA | NZ_CP022686 | 496,074 | G→T | 8.8% | P403T (CCC→ACC) | otsA ← | alpha,alpha‑trehalose‑phosphate synthase |
| RA | NZ_CP022686 | 498,647 | C→A | 8.5% | G188C (GGC→TGC) | araH ← | arabinose ABC transporter permease AraH |
| RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
| RA | NZ_CP022686 | 596,637 | T→G | 10.3% | G54G (GGT→GGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| RA | NZ_CP022686 | 601,295 | T→G | 18.5% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| RA | NZ_CP022686 | 601,302 | T→G | 21.3% | R1609R (CGT→CGG) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
| MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
| RA | NZ_CP022686 | 651,848 | A→C | 10.5% | R238R (CGT→CGG) | clbJ ← | colibactin non‑ribosomal peptide synthetase ClbJ |
| RA | NZ_CP022686 | 692,689 | T→G | 9.0% | F10C (TTT→TGT) | CIW80_RS03550 → | YaiO family outer membrane beta‑barrel protein |
| RA | NZ_CP022686 | 693,001 | A→C | 7.9% | N114T (AAC→ACC) | CIW80_RS03550 → | YaiO family outer membrane beta‑barrel protein |
| RA | NZ_CP022686 | 693,568 | G→T | 7.5% | intergenic (+134/‑334) | CIW80_RS03550 → / → CIW80_RS03555 | YaiO family outer membrane beta‑barrel protein/transposase |
| RA | NZ_CP022686 | 694,391 | C→A | 13.8% | intergenic (+151/‑45) | CIW80_RS03555 → / → CIW80_RS03560 | transposase/PfkB family carbohydrate kinase |
| RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
| RA | NZ_CP022686 | 764,997 | T→G | 13.1% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
| RA | NZ_CP022686 | 765,008 | A→T | 11.1% | I37I (ATT→ATA) | wcaM ← | colanic acid biosynthesis protein WcaM |
| RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
| RA | NZ_CP022686 | 829,441 | C→A | 21.3% | intergenic (‑30/+197) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 829,452 | G→T | 21.1% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 829,467 | C→A | 25.4% | intergenic (‑56/+171) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 829,482 | C→A | 8.9% | intergenic (‑71/+156) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
| RA | NZ_CP022686 | 837,555 | T→G | 9.8% | intergenic (‑128/+95) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
| RA | NZ_CP022686 | 837,589 | A→T | 10.7% | intergenic (‑162/+61) | thiM ← / ← rcnR | hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR |
| RA | NZ_CP022686 | 857,274 | T→G | 8.9% | G78G (GGT→GGG) | yehL → | MoxR family ATPase |
| RA | NZ_CP022686 | 881,733 | A→C | 13.8% | intergenic (+248/+139) | yohP → / ← dusC | protein YohP/tRNA dihydrouridine(16) synthase DusC |
| RA | NZ_CP022686 | 881,739 | A→C | 15.5% | intergenic (+254/+133) | yohP → / ← dusC | protein YohP/tRNA dihydrouridine(16) synthase DusC |
| RA | NZ_CP022686 | 881,760 | T→G | 12.7% | intergenic (+275/+112) | yohP → / ← dusC | protein YohP/tRNA dihydrouridine(16) synthase DusC |
| RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
| RA | NZ_CP022686 | 944,572 | A→C | 7.2% | T88P (ACG→CCG) | CIW80_RS04825 → | single‑stranded DNA‑binding protein |
| RA | NZ_CP022686 | 948,051 | G→T | 13.1% | intergenic (+120/‑137) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
| RA | NZ_CP022686 | 948,080 | G→T | 13.8% | intergenic (+149/‑108) | CIW80_RS04855 → / → CIW80_RS04860 | terminase small subunit/hypothetical protein |
| RA | NZ_CP022686 | 957,112 | T→G | 10.0% | T89P (ACC→CCC) | ccmA ← | cytochrome c biogenesis heme‑transporting ATPase CcmA |
| RA | NZ_CP022686 | 1,000,401 | A→C | 15.8% | V955G (GTG→GGG) | yfaL ← | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| RA | NZ_CP022686 | 1,000,439 | T→G | 23.4% | P942P (CCA→CCC) | yfaL ← | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| RA | NZ_CP022686 | 1,005,085 | C→A | 8.6% | T380T (ACC→ACA) | nrdA → | ribonucleoside‑diphosphate reductase subunit alpha |
| RA | NZ_CP022686 | 1,065,984 | C→A | 9.0% | V35F (GTC→TTC) | yfbS ← | SLC13 family permease |
| RA | NZ_CP022686 | 1,065,997 | A→C | 9.3% | A30A (GCT→GCG) | yfbS ← | SLC13 family permease |
| RA | NZ_CP022686 | 1,145,570 | C→G | 7.9% | L333F (TTG→TTC) | oxc ← | oxalyl‑CoA decarboxylase |
| RA | NZ_CP022686 | 1,171,408 | C→G | 7.7% | intergenic (‑26/+14) | CIW80_RS05830 ← / ← CIW80_RS05835 | tRNA‑Ala/tRNA‑Ala |
| RA | NZ_CP022686 | 1,174,272 | A→C | 14.5% | intergenic (+1/‑43) | CIW80_RS05855 → / → CIW80_RS05860 | tRNA‑Val/tRNA‑Val |
| RA | NZ_CP022686 | 1,174,416 | A→T | 8.2% | intergenic (+26/‑21) | CIW80_RS05860 → / → CIW80_RS05865 | tRNA‑Val/tRNA‑Val |
| RA | NZ_CP022686 | 1,197,142 | T→G | 7.6% | G74G (GGT→GGG) | murQ → | N‑acetylmuramic acid 6‑phosphate etherase |
| RA | NZ_CP022686 | 1,360,709 | T→G | 12.2% | intergenic (‑44/+280) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,724 | G→T | 11.4% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,727 | A→T | 7.8% | intergenic (‑62/+262) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,729 | G→T | 7.9% | intergenic (‑64/+260) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,742 | C→A | 7.2% | intergenic (‑77/+247) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,751 | C→G | 7.4% | intergenic (‑86/+238) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,360,756 | C→A | 13.6% | intergenic (‑91/+233) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
| RA | NZ_CP022686 | 1,366,299 | C→G | 6.4% | intergenic (‑300/+142) | CIW80_RS06740 ← / ← clpB | 16S ribosomal RNA/ATP‑dependent chaperone ClpB |
| RA | NZ_CP022686 | 1,370,556 | T→G | 19.4% | P99P (CCA→CCC) | rluD ← | 23S rRNA pseudouridine(1911/1915/1917) synthase RluD |
| RA | NZ_CP022686 | 1,411,220 | A→C | 5.9% | D321A (GAC→GCC) | proW → | glycine betaine/L‑proline ABC transporter permease ProW |
| RA | NZ_CP022686 | 1,433,681 | G→T | 7.6% | E95* (GAA→TAA) | srlB → | PTS glucitol/sorbitol transporter subunit IIA |
| RA | NZ_CP022686 | 1,479,607 | A→C | 11.7% | intergenic (‑16/+177) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
| RA | NZ_CP022686 | 1,479,625 | G→C | 7.4% | intergenic (‑34/+159) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
| RA | NZ_CP022686 | 1,500,012 | C→A | 8.6% | P257T (CCG→ACG) | ygcE → | FGGY‑family carbohydrate kinase |
| RA | NZ_CP022686 | 1,504,339 | C→A | 20.7% | P259Q (CCA→CAA) | CIW80_RS07520 → | YgcG family protein |
| RA | NZ_CP022686 | 1,504,361 | G→T | 20.8% | V266V (GTG→GTT) | CIW80_RS07520 → | YgcG family protein |
| RA | NZ_CP022686 | 1,535,307 | A→C | 8.3% | D16A (GAC→GCC) | fucI → | L‑fucose isomerase |
| RA | NZ_CP022686 | 1,535,365 | A→C | 22.7% | K35N (AAA→AAC) | fucI → | L‑fucose isomerase |
| RA | NZ_CP022686 | 1,558,390 | G→T | 11.5% | V463L (GTA→TTA) | vgrG → | type VI secretion system Vgr family protein |
| RA | NZ_CP022686 | 1,558,395 | A→C | 9.6% | K464N (AAA→AAC) | vgrG → | type VI secretion system Vgr family protein |
| RA | NZ_CP022686 | 1,632,480 | T→G | 7.1% | V159G (GTC→GGC) | arcC → | carbamate kinase |
| RA | NZ_CP022686 | 1,632,501 | T→G | 9.0% | V166G (GTT→GGT) | arcC → | carbamate kinase |
| RA | NZ_CP022686 | 1,632,512 | G→T | 9.4% | E170* (GAA→TAA) | arcC → | carbamate kinase |
| RA | NZ_CP022686 | 1,633,846 | T→G | 27.0% | N253T (AAC→ACC) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
| RA | NZ_CP022686 | 1,633,870 | G→T | 8.8% | A245E (GCA→GAA) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
| RA | NZ_CP022686 | 1,674,224 | T→G | 7.3% | K188N (AAA→AAC) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,674,231 | A→T | 7.4% | V186E (GTA→GAA) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,674,248 | T→G | 14.0% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
| RA | NZ_CP022686 | 1,705,997 | C→A | 12.3% | intergenic (‑607/‑48) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,706,025 | C→A | 12.0% | intergenic (‑635/‑20) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,706,032 | G→T | 15.0% | intergenic (‑642/‑13) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,706,047 | G→T | 13.7% | M1M (ATG→ATT) † | metK → | methionine adenosyltransferase |
| RA | NZ_CP022686 | 1,710,453 | A→C | 7.0% | intergenic (+48/‑32) | endA → / → rsmE | deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase |
| RA | NZ_CP022686 | 1,716,145 | A→C | 9.5% | T42P (ACC→CCC) | rdgB → | XTP/dITP diphosphatase |
| RA | NZ_CP022686 | 1,828,110 | A→C | 5.8% | R85R (CGT→CGG) | gspK ← | type II secretion system minor pseudopilin GspK |
| RA | NZ_CP022686 | 1,954,932 | G→T | 8.0% | Q89K (CAA→AAA) | ttdR ← | DNA‑binding transcriptional activator TtdR |
| RA | NZ_CP022686 | 2,037,521 | A→C | 9.6% | T508P (ACC→CCC) | lpoA → | penicillin‑binding protein activator LpoA |
| RA | NZ_CP022686 | 2,049,089 | T→G | 11.1% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase DeaD |
| RA | NZ_CP022686 | 2,049,094 | G→T | 9.8% | T254T (ACC→ACA) | deaD ← | ATP‑dependent RNA helicase DeaD |
| RA | NZ_CP022686 | 2,056,168 | A→C | 8.9% | G614G (GGT→GGG) | infB ← | translation initiation factor IF‑2 |
| RA | NZ_CP022686 | 2,056,193 | A→C | 6.4% | V606G (GTT→GGT) | infB ← | translation initiation factor IF‑2 |
| RA | NZ_CP022686 | 2,110,457 | T→G | 13.7% | K12N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
| RA | NZ_CP022686 | 2,110,472 | T→G | 7.6% | K7N (AAA→AAC) | rplM ← | 50S ribosomal protein L13 |
| RA | NZ_CP022686 | 2,130,829 | A→C | 8.8% | V237G (GTT→GGT) | mreC ← | rod shape‑determining protein MreC |
| RA | NZ_CP022686 | 2,148,115 | T→G | 10.5% | L126R (CTG→CGG) | panF → | sodium/pantothenate symporter |
| RA | NZ_CP022686 | 2,148,118 | C→G | 12.5% | A127G (GCG→GGG) | panF → | sodium/pantothenate symporter |
| RA | NZ_CP022686 | 2,172,177 | A→C | 7.1% | G87G (GGT→GGG) | smg ← | DUF494 family protein Smg |
| RA | NZ_CP022686 | 2,185,075 | A→C | 7.1% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
| RA | NZ_CP022686 | 2,192,062 | A→C | 8.2% | R69R (CGT→CGG) | rplD ← | 50S ribosomal protein L4 |
| RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
| RA | NZ_CP022686 | 2,226,107 | A→C | 7.1% | intergenic (‑295/‑7) | yhfA ← / → crp | OsmC family protein/cAMP‑activated global transcriptional regulator CRP |
| RA | NZ_CP022686 | 2,226,139 | A→C | 8.0% | D9A (GAC→GCC) | crp → | cAMP‑activated global transcriptional regulator CRP |
| RA | NZ_CP022686 | 2,272,831 | G→T | 7.4% | intergenic (+73/‑24) | greB → / → yhgF | transcription elongation factor GreB/Tex family protein |
| RA | NZ_CP022686 | 2,310,429 | T→G | 7.5% | N248T (AAC→ACC) | glgP ← | glycogen phosphorylase |
| RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
| RA | NZ_CP022686 | 2,337,641 | A→C | 5.6% | V124G (GTA→GGA) | ugpA ← | sn‑glycerol‑3‑phosphate ABC transporter permease UgpA |
| RA | NZ_CP022686 | 2,370,472 | C→A | 5.2% | R359S (CGC→AGC) | CIW80_RS11930 → | FGGY family carbohydrate kinase |
| RA | NZ_CP022686 | 2,380,936 | A→C | 8.7% | K186N (AAA→AAC) | pitA → | inorganic phosphate transporter PitA |
| RA | NZ_CP022686 | 2,394,287 | T→G | 13.1% | N115T (AAC→ACC) | chuS ← | hematinate‑forming heme oxygenase ChuS |
| RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
| RA | NZ_CP022686 | 2,460,523 | A→C | 8.0% | V19G (GTA→GGA) | yhjY ← | autotransporter outer membrane beta‑barrel domain‑containing protein |
| RA | NZ_CP022686 | 2,460,576 | C→A | 14.8% | M1M (ATG→ATT) † | yhjY ← | autotransporter outer membrane beta‑barrel domain‑containing protein |
| RA | NZ_CP022686 | 2,460,581 | A→C | 21.0% | intergenic (‑3/‑155) | yhjY ← / → tag | autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I |
| RA | NZ_CP022686 | 2,470,422 | T→G | 9.2% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
| RA | NZ_CP022686 | 2,499,319 | T→G | 5.7% | N44T (AAC→ACC) | aldB ← | aldehyde dehydrogenase AldB |
| RA | NZ_CP022686 | 2,516,872 | A→C | 6.8% | K761T (AAA→ACA) | upaG → | trimeric autotransporter adhesin UpaG |
| RA | NZ_CP022686 | 2,517,086 | A→C | 6.6% | K832N (AAA→AAC) | upaG → | trimeric autotransporter adhesin UpaG |
| RA | NZ_CP022686 | 2,550,305 | T→G | 6.8% | K151N (AAA→AAC) | mutM ← | bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase |
| RA | NZ_CP022686 | 2,555,413 | A→C | 18.1% | intergenic (‑40/+26) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH |
| RA | NZ_CP022686 | 2,559,398 | T→G | 5.9% | N461T (AAC→ACC) | ligB ← | NAD‑dependent DNA ligase LigB |
| RA | NZ_CP022686 | 2,572,705 | A→C | 11.2% | G191G (GGT→GGG) | CIW80_RS12885 ← | ROK family protein |
| JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
| RA | NZ_CP022686 | 2,703,299 | A→C | 11.1% | intergenic (‑203/‑277) | yieP ← / → CIW80_RS13540 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| RA | NZ_CP022686 | 2,733,571 | T→G | 8.4% | F53V (TTT→GTT) | rffG → | dTDP‑glucose 4,6‑dehydratase |
| RA | NZ_CP022686 | 2,736,585 | T→A | 9.0% | Y183N (TAC→AAC) | rffA → | dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase |
| RA | NZ_CP022686 | 2,743,563 | T→G | 9.8% | noncoding (65/87 nt) | CIW80_RS13750 → | tRNA‑Leu |
| RA | NZ_CP022686 | 2,758,113 | A→C | 7.2% | A35A (GCA→GCC) | yifL → | lipoprotein |
| RA | NZ_CP022686 | 2,758,128 | A→C | 10.7% | K40N (AAA→AAC) | yifL → | lipoprotein |
| RA | NZ_CP022686 | 2,758,144 | A→C | 7.1% | T46P (ACG→CCG) | yifL → | lipoprotein |
| RA | NZ_CP022686 | 2,769,186 | C→A | 11.2% | M50I (ATG→ATT) | rarD ← | EamA family transporter RarD |
| RA | NZ_CP022686 | 2,769,196 | A→C | 15.5% | F47C (TTT→TGT) | rarD ← | EamA family transporter RarD |
| RA | NZ_CP022686 | 2,780,110 | T→G | 6.4% | F677C (TTT→TGT) | metE → | 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase |
| RA | NZ_CP022686 | 2,780,127 | C→A | 8.8% | P683T (CCA→ACA) | metE → | 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase |
| RA | NZ_CP022686 | 2,780,133 | G→T | 6.3% | E685* (GAA→TAA) | metE → | 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase |
| RA | NZ_CP022686 | 2,801,864 | T→G | 5.9% | L16F (TTA→TTC) | CIW80_RS14030 ← | 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase |
| RA | NZ_CP022686 | 2,808,127 | T→G | 9.7% | V441G (GTC→GGC) | ubiB → | ubiquinone biosynthesis regulatory protein kinase UbiB |
| RA | NZ_CP022686 | 2,808,142 | T→G | 10.2% | L446R (CTG→CGG) | ubiB → | ubiquinone biosynthesis regulatory protein kinase UbiB |
| RA | NZ_CP022686 | 2,808,145 | T→G | 7.5% | V447G (GTG→GGG) | ubiB → | ubiquinone biosynthesis regulatory protein kinase UbiB |
| RA | NZ_CP022686 | 2,821,644 | A→C | 8.8% | intergenic (+100/‑278) | hemG → / → CIW80_RS14130 | menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA |
| RA | NZ_CP022686 | 2,868,740 | T→G | 25.0% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
| RA | NZ_CP022686 | 2,868,753 | T→G | 11.4% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
| RA | NZ_CP022686 | 2,868,754 | C→G | 9.3% | L17L (CTG→CTC) | fdoH ← | formate dehydrogenase O subunit beta |
| RA | NZ_CP022686 | 2,871,417 | C→A | 7.3% | E151* (GAA→TAA) | fdnG ← | formate dehydrogenase‑N subunit alpha |
| RA | NZ_CP022686 | 2,871,422 | T→G | 6.4% | N149T (AAC→ACC) | fdnG ← | formate dehydrogenase‑N subunit alpha |
| RA | NZ_CP022686 | 2,908,932 | A→C | 7.6% | F36V (TTT→GTT) | priA ← | primosomal protein N' |
| RA | NZ_CP022686 | 2,920,385 | C→A | 6.3% | A54E (GCA→GAA) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| RA | NZ_CP022686 | 2,920,428 | A→C | 9.0% | K68N (AAA→AAC) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| RA | NZ_CP022686 | 2,961,318 | A→C | 8.3% | intergenic (+96/‑277) | murI → / → CIW80_RS14765 | glutamate racemase/16S ribosomal RNA |
| RA | NZ_CP022686 | 2,981,624 | G→C | 10.8% | D571H (GAC→CAC) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| RA | NZ_CP022686 | 3,017,622 | A→C | 10.8% | K839N (AAA→AAC) | metH → | methionine synthase |
| RA | NZ_CP022686 | 3,020,229 | T→G | 13.6% | V133G (GTC→GGC) | yjbB → | Na/Pi cotransporter family protein |
| RA | NZ_CP022686 | 3,071,004 | T→G | 5.9% | G59G (GGT→GGG) | CIW80_RS15290 → | 2‑oxoglutarate dehydrogenase E1 component |
| RA | NZ_CP022686 | 3,136,940 | T→G | 9.8% | D161A (GAC→GCC) | phnN ← | ribose 1,5‑bisphosphokinase |
| JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
| RA | NZ_CP022686 | 3,200,595 | A→C | 7.0% | V199G (GTT→GGT) | frdA ← | fumarate reductase (quinol) flavoprotein subunit |
| RA | NZ_CP022686 | 3,222,426 | T→G | 5.9% | R272R (CGT→CGG) | hflC → | protease modulator HflC |
| RA | NZ_CP022686 | 3,281,004 | T→G | 8.1% | F75V (TTT→GTT) | yjfF → | sugar ABC transporter permease YjfF |
| RA | NZ_CP022686 | 3,286,882 | C→A | 6.8% | Q63K (CAA→AAA) | cybC → | cytochrome b562 |
| RA | NZ_CP022686 | 3,348,342 | A→T | 8.1% | intergenic (‑158/‑42) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
| RA | NZ_CP022686 | 3,348,347 | A→T | 7.9% | intergenic (‑163/‑37) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
| RA | NZ_CP022686 | 3,348,357 | T→G | 8.7% | intergenic (‑173/‑27) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
| RA | NZ_CP022686 | 3,363,064 | C→A | 6.5% | intergenic (+763/‑28) | CIW80_RS16815 → / → CIW80_RS16820 | helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase |
| RA | NZ_CP022686 | 3,376,248 | A→C | 5.8% | K152N (AAA→AAC) | mtnA → | S‑methyl‑5‑thioribose‑1‑phosphate isomerase |
| RA | NZ_CP022686 | 3,387,116 | A→C | 13.1% | F86V (TTT→GTT) | sgcA ← | SgcA family PTS sugar transporter subunit IIA |
| RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
| RA | NZ_CP022686 | 3,444,704 | T→G | 8.9% | T530P (ACC→CCC) | btsT ← | pyruvate/proton symporter BtsT |
| RA | NZ_CP022686 | 3,451,845 | A→C | 10.1% | E278D (GAA→GAC) | lgoD → | L‑galactonate oxidoreductase |
| RA | NZ_CP022686 | 3,493,370 | T→G | 5.9% | K195N (AAA→AAC) | arcA ← | two‑component system response regulator ArcA |
| RA | NZ_CP022686 | 3,516,075 | T→G | 6.8% | V213G (GTG→GGG) | nhaA → | Na+/H+ antiporter NhaA |
| MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | 100% | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
| RA | NZ_CP022686 | 3,570,252 | T→G | 5.9% | L223V (TTG→GTG) | yabI → | DedA family protein |
| RA | NZ_CP022686 | 3,570,313 | T→G | 6.4% | V243G (GTG→GGG) | yabI → | DedA family protein |
| RA | NZ_CP022686 | 3,628,882 | A→C | 9.0% | D324A (GAC→GCC) | aceF → | pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase |
| RA | NZ_CP022686 | 3,652,079 | T→G | 6.2% | intergenic (‑88/+24) | panB ← / ← CIW80_RS18255 | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin |
| RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
| RA | NZ_CP022686 | 3,734,367 | T→G | 13.6% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
| RA | NZ_CP022686 | 3,770,508 | T→G | 9.0% | intergenic (‑244/+219) | CIW80_RS26265 ← / ← CIW80_RS18890 | ATP‑binding protein/hypothetical protein |
| RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
| RA | NZ_CP022686 | 3,797,483 | A→T | 11.5% | L130F (TTA→TTT) | CIW80_RS19065 → | LacI family DNA‑binding transcriptional regulator |
| RA | NZ_CP022686 | 3,800,981 | A→C | 6.5% | pseudogene (1257/1773 nt) | CIW80_RS19080 → | ShlB/FhaC/HecB family hemolysin secretion/activation protein |
| RA | NZ_CP022686 | 3,806,214 | G→T | 7.9% | E1569* (GAA→TAA) | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
| RA | NZ_CP022686 | 3,806,228 | T→A | 7.2% | N1573K (AAT→AAA) | cdiA → | contact‑dependent inhibition effector tRNA nuclease |
| MC JC | NZ_CP022686 | 3,844,426 | Δ1,036 bp | 100% | coding (2‑1037/1056 nt) | dinB → | DNA polymerase IV |
| RA | NZ_CP022686 | 3,868,846 | T→G | 12.7% | T383P (ACG→CCG) | ecpC ← | fimbrial usher EcpC |
| RA | NZ_CP022686 | 3,868,857 | T→G | 6.4% | N379T (AAC→ACC) | ecpC ← | fimbrial usher EcpC |
| RA | NZ_CP022686 | 3,868,864 | A→C | 11.3% | S377A (TCG→GCG) | ecpC ← | fimbrial usher EcpC |
| RA | NZ_CP022686 | 3,885,449 | G→C | 10.4% | V1401V (GTG→GTC) | fdeC → | inverse autotransporter adhesin FdeC |
| RA | NZ_CP022686 | 3,895,534 | T→G | 10.2% | intergenic (‑140/+44) | CIW80_RS19510 ← / ← CIW80_RS19515 | protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein |
| RA | NZ_CP022686 | 3,925,289 | A→C | 19.0% | N156T (AAC→ACC) | prpC → | 2‑methylcitrate synthase |
| RA | NZ_CP022686 | 3,928,369 | T→G | 11.2% | W285G (TGG→GGG) | prpE → | propionate‑‑CoA ligase |
| RA | NZ_CP022686 | 3,928,376 | T→G | 5.4% | V287G (GTA→GGA) | prpE → | propionate‑‑CoA ligase |
| RA | NZ_CP022686 | 3,932,475 | A→C | 9.1% | V173G (GTG→GGG) | lacA ← | galactoside O‑acetyltransferase |
| RA | NZ_CP022686 | 3,935,324 | T→G | 12.8% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
| RA | NZ_CP022686 | 3,940,724 | A→C | 9.6% | V308G (GTA→GGA) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
| RA | NZ_CP022686 | 3,942,556 | T→G | 7.6% | T121P (ACG→CCG) | yaiO ← | YaiO family outer membrane beta‑barrel protein |
| RA | NZ_CP022686 | 3,949,969 | T→G | 9.8% | D141A (GAC→GCC) | hemB ← | porphobilinogen synthase |
| RA | NZ_CP022686 | 3,979,381 | T→G | 7.7% | L96R (CTT→CGT) | brnQ → | branched‑chain amino acid transporter carrier protein BrnQ |
| RA | NZ_CP022686 | 3,981,933 | T→G | 11.2% | intergenic (+70/‑86) | proY → / → malZ | proline‑specific permease ProY/maltodextrin glucosidase |
| RA | NZ_CP022686 | 4,014,238 | C→G | 12.2% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
| RA | NZ_CP022686 | 4,014,242 | A→T | 8.3% | intergenic (+88/‑256) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
| RA | NZ_CP022686 | 4,019,028 | A→C | 7.4% | D259A (GAC→GCC) | lon → | endopeptidase La |
| RA | NZ_CP022686 | 4,056,962 | C→A | 15.8% | F137L (TTC→TTA) | adk → | adenylate kinase |
| RA | NZ_CP022686 | 4,079,363 | A→C | 13.6% | A158A (GCT→GCG) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
| RA | NZ_CP022686 | 4,287,491 | T→G | 9.1% | noncoding (4/76 nt) | CIW80_RS21480 → | tRNA‑Lys |
| RA | NZ_CP022686 | 4,368,341 | T→G | 12.0% | D47A (GAC→GCC) | ybiY ← | glycyl‑radical enzyme activating protein |
| RA | NZ_CP022686 | 4,368,380 | T→G | 8.6% | N34T (AAC→ACC) | ybiY ← | glycyl‑radical enzyme activating protein |
| RA | NZ_CP022686 | 4,440,324 | C→A | 23.1% | P794Q (CCG→CAG) | ftsK → | DNA translocase FtsK |
| RA | NZ_CP022686 | 4,440,334 | A→C | 17.0% | Q797H (CAA→CAC) | ftsK → | DNA translocase FtsK |
| RA | NZ_CP022686 | 4,485,614 | T→G | 7.1% | F139V (TTT→GTT) | gloC → | hydroxyacylglutathione hydrolase GloC |
| RA | NZ_CP022686 | 4,535,343 | T→A | 5.3% | R116R (CGT→CGA) | appA → | bifunctional acid phosphatase/4‑phytase |
| RA | NZ_CP022686 | 4,632,182 | T→G | 7.1% | R276R (CGT→CGG) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
| RA | NZ_CP022686 | 4,678,047 | A→C | 29.6% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
| RA | NZ_CP022686 | 4,759,410 | G→T | 10.2% | F207L (TTC→TTA) | ldtC ← | L,D‑transpeptidase LdtC |
| RA | NZ_CP022686 | 4,764,877 | T→G | 11.8% | intergenic (‑57/‑204) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
| RA | NZ_CP022686 | 4,764,886 | G→T | 10.8% | intergenic (‑66/‑195) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
| RA | NZ_CP022686 | 4,815,879 | T→G | 13.9% | F23V (TTC→GTC) | CIW80_RS24305 → | C40 family peptidase |
| RA | NZ_CP022686 | 4,815,883 | T→G | 8.4% | V24G (GTG→GGG) | CIW80_RS24305 → | C40 family peptidase |
| RA | NZ_CP022686 | 4,839,687 | T→G | 14.5% | N144T (AAC→ACC) | minD ← | septum site‑determining protein MinD |
| MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | 100% | [umuD]–[umuC] | [umuD], [umuC] | |
| RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
| RA | NZ_CP022686 | 4,905,170 | T→G | 17.9% | V99G (GTT→GGT) | CIW80_RS24815 → | coenzyme F390 synthetase |
| RA | NZ_CP022686 | 4,916,128 | A→C | 6.7% | intergenic (+255/+30) | tdk → / ← CIW80_RS24885 | thymidine kinase/IS4 family transposase |
| RA | NZ_CP022686 | 4,918,837 | T→G | 7.7% | K390N (AAA→AAC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
| RA | NZ_CP022686 | 4,918,846 | C→A | 12.7% | Q387H (CAG→CAT) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
| RA | NZ_CP022686 | 4,951,545 | A→C | 11.0% | S47R (AGT→CGT) | topA → | type I DNA topoisomerase |
| RA | NZ_CP022686 | 5,001,157 | G→T | 18.8% | D161Y (GAT→TAT) | ompG → | monomeric porin OmpG |
| RA | NZ_CP022686 | 5,042,250 | A→T | 7.2% | V627V (GTA→GTT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,042,304 | T→G | 11.9% | N645K (AAT→AAG) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,043,648 | G→C | 6.4% | V1093V (GTG→GTC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,043,855 | T→A | 10.5% | G1162G (GGT→GGA) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,043,879 | C→T | 6.4% | G1170G (GGC→GGT) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | NZ_CP022686 | 5,044,614 | T→C | 8.3% | G1415G (GGT→GGC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
| RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
| JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] | |
| RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,645 | C→T | 55.7% | Q13* (CAG→TAG) | IL2wt no signal → | |
| RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,762 | G→T | 21.0% | E52* (GAA→TAA) | IL2wt no signal → | |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NZ_MW240712 | 187–2618 | 3086–2957 | 340–2900 | 13107 [12562] | [11892] 13178 | JNG15_RS00005–mobA | JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA |
| * | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4753–4878 | 5478–5073 | 196–726 | 5497 [5108] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP022686 | 262466 = | 4 (0.020) | 191 (0.880) | 63/246 | NT | 97.9% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
| ? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
| * | ? | NZ_CP022686 | = 262468 | 5 (0.020) | 183 (0.850) | 59/246 | NT | 97.3% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
| ? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase | |||||
| * | ? | NZ_CP022686 | 1522881 = | NA (NA) | 27 (0.010) | 16/246 | NT | 69.2% | intergenic (‑394/‑72) | yqcC/CIW80_RS07590 | YqcC family protein/IS3‑like element ISEc52 family transposase |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3610 = | 12 (0.000) | coding (2/399 nt) | IL2wt no signal | ||||||
| * | ? | NZ_CP022686 | = 1524130 | NA (NA) | 50 (0.010) +24 bp |
26/198 | NT | 83.8% | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3594 | 12 (0.000) | intergenic (+1/‑15) | ompA/IL2wt no signal | –/– | |||||
| * | ? | NZ_CP022686 | = 1524131 | NA (NA) | 29 (0.010) +GGG |
21/240 | NT | 71.2% | intergenic (+9/+231) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3594 | 12 (0.000) | intergenic (+1/‑15) | ompA/IL2wt no signal | –/– | |||||
| * | ? | NZ_CP022686 | 3899666 = | 219 (1.010) | 58 (0.310) | 26/214 | NT | 24.4% | intergenic (‑40/+290) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
| ? | NZ_CP022686 | 3899944 = | 168 (0.890) | intergenic (‑318/+12) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
| * | ? | NZ_CP022686 | = 3899681 | 218 (1.010) | 55 (0.290) | 32/214 | NT | 23.5% | intergenic (‑55/+275) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
| ? | NZ_CP022686 | = 3899927 | 168 (0.890) | intergenic (‑301/+29) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
| * | ? | NZ_CP022686 | 4514374 = | 205 (0.950) | 7 (0.000) +AGCT |
6/238 | NT | 6.3% | coding (59/1053 nt) | ompA | porin OmpA |
| ? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3609 = | 12 (0.000) | coding (1/399 nt) | IL2wt no signal | ||||||