breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 12.0% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 192,380 T→A 7.4% R597R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 238,279 G→T 9.2% F100L (TTC→TTA sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 238,313 T→A 14.4% Y89F (TAC→TTC)  sufE ← cysteine desulfuration protein SufE
RA NZ_CP022686 298,027 A→C 9.6% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 349,100 A→C 18.7% T605P (ACG→CCG)  yeaG → protein kinase YeaG
RA NZ_CP022686 355,148 T→G 5.6% G139G (GGT→GGG
D270A (GAC→GCC) 
yeaL →
nimR ←
DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR
RA NZ_CP022686 360,227 T→G 16.0% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,246 T→G 8.2% E28D (GAA→GAC yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 19.4% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 377,307 A→C 9.6% K147N (AAA→AAC manX → PTS mannose transporter subunit IIAB
RA NZ_CP022686 418,374 T→G 10.0% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 556,940 A→T 11.9% intergenic (+124/‑724) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 599,447 A→C 8.2% D991A (GAC→GCC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 17.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 14.0% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 693,001 A→C 10.6% N114T (AAC→ACC)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 693,021 A→C 9.2% T121P (ACG→CCG)  CIW80_RS03550 → YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 22.5% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,448 T→A 11.5% intergenic (‑37/+190) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 20.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 19.9% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 17.1% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 837,561 G→T 14.0% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 881,739 A→C 17.2% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,080 G→T 14.7% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 17.0% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 9.9% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 14.1% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,094 G→C 10.5% E383D (GAG→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,397 T→A 9.2% intergenic (‑246/‑27) glpT ← / → glpA glycerol‑3‑phosphate transporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,034,493 T→G 8.4% T26P (ACC→CCC)  menC ← o‑succinylbenzoate synthase
RA NZ_CP022686 1,040,909 A→C 9.9% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,047,883 T→G 10.6% intergenic (‑113/+51) nuoM ← / ← nuoL NADH‑quinone oxidoreductase subunit M/NADH‑quinone oxidoreductase subunit L
RA NZ_CP022686 1,065,997 A→C 14.2% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,195,277 T→G 5.5% D139A (GAC→GCC)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA NZ_CP022686 1,213,479 T→G 15.1% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,360,709 T→G 15.1% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 12.6% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 7.7% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 9.9% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,559 T→G 12.1% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,081 C→A 14.9% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,479,607 A→C 10.0% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,500,012 C→A 10.0% P257T (CCG→ACG)  ygcE → FGGY‑family carbohydrate kinase
RA NZ_CP022686 1,504,299 G→T 11.5% V246F (GTT→TTT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 15.6% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,558,395 A→C 10.2% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 10.0% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,597,235 T→G 10.2% N19H (AAC→CAC)  thyA ← thymidylate synthase
RA NZ_CP022686 1,604,984 A→C 11.0% V338G (GTC→GGC)  lplT ← lysophospholipid transporter LplT
RA NZ_CP022686 1,674,244 T→G 16.2% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 13.1% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,796,792 A→C 7.0% intergenic (+5/‑90) agn43 → / → CIW80_RS08945 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 1,911,580 G→T 14.3% S231R (AGC→AGA CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,911,602 T→G 10.8% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 2,016,225 T→G 9.6% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,046,671 A→C 5.7% V381G (GTC→GGC)  mtr ← tryptophan permease
RA NZ_CP022686 2,076,554 T→G 12.8% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,090,800 T→G 8.3% S166A (TCG→GCG)  yrbL → PhoP regulatory network protein YrbL
RA NZ_CP022686 2,110,472 T→G 7.6% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,490 C→A 11.6% M1M (ATG→ATT) † rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,234 T→A 6.5% Y210F (TAT→TTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,123,241 A→C 5.9% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,148,103 T→G 22.6% L122R (CTT→CGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,136 T→G 9.8% V133G (GTT→GGT)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,139 C→G 11.5% A134G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,149 T→G 9.6% G137G (GGT→GGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,673 C→A 14.6% A167E (GCA→GAA)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,185,075 A→C 8.0% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 7.4% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,250,454 A→C 7.1% S40A (TCG→GCG)  dam ← adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 2,272,831 G→T 7.0% intergenic (+73/‑24) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,357,322 T→A 13.0% S36R (AGT→AGA dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,361,489 T→G 11.3% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein
RA NZ_CP022686 2,380,585 T→G 5.2% G69G (GGT→GGG pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,397,400 C→A 9.9% S19* (TCA→TAA)  chuT → heme ABC transporter substrate‑binding protein ChuT
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,448,935 C→A 7.7% F187L (TTC→TTA yhjV → aromatic amino acid transport family protein
RA NZ_CP022686 2,460,576 C→A 12.7% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 9.5% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 7.6% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,743,563 T→G 12.1% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,758,128 A→C 6.4% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,769,196 A→C 14.6% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,820,583 A→C 5.7% T350P (ACG→CCG)  trkH → Trk system potassium transporter TrkH
RA NZ_CP022686 2,821,644 A→C 7.3% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,834,886 A→C 10.9% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 19.3% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,742 A→C 13.8% P21P (CCT→CCG fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,852 T→G 8.7% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,981,612 A→C 8.1% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 10.6% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,184,651 A→C 6.8% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 8.2% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,218,260 T→G 6.8% L233R (CTT→CGT)  miaA → tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
RA NZ_CP022686 3,387,116 A→C 9.7% F86V (TTT→GTT)  sgcA ← SgcA family PTS sugar transporter subunit IIA
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,215 A→T 17.0% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 12.2% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,221 T→G 14.7% intergenic (‑30/+5) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 24.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,468,357 A→C 9.5% T300P (ACG→CCG)  yjjU → patatin family protein
RA NZ_CP022686 3,516,058 T→G 10.2% V207V (GTT→GTG nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,598,457 T→G 9.0% V228G (GTG→GGG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA NZ_CP022686 3,652,090 T→G 9.8% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 14.0% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,714,342 T→G 8.5% L275V (TTG→GTG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,734,367 T→G 24.3% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 17.6% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,868,864 A→C 8.4% S377A (TCG→GCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,897,759 A→T 8.7% pseudogene (117/1083 nt) hyxB ← outer membrane autotransporter HyxB
RA NZ_CP022686 3,910,255 G→T 6.4% F69L (TTC→TTA yahB ← LysR substrate‑binding domain‑containing protein
RA NZ_CP022686 3,910,616 C→A 10.5% G145G (GGG→GGT yahC ← DUF1097 domain‑containing protein
RA NZ_CP022686 3,925,309 G→T 9.4% E163* (GAA→TAA)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 8.1% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 12.3% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 10.4% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,950,011 T→G 6.7% H127P (CAC→CCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 11.5% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 13.9% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 14.3% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,019,028 A→C 9.4% D259A (GAC→GCC)  lon → endopeptidase La
RA NZ_CP022686 4,032,237 A→C 6.6% D88A (GAC→GCC)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,032,257 G→T 10.2% E95* (GAA→TAA)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,079,344 C→A 12.3% A165S (GCG→TCG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,196,276 T→G 17.5% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,214,439 G→C 8.9% intergenic (‑12/+23) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,214,457 G→T 11.1% intergenic (‑30/+5) CIW80_RS21095 ← / ← CIW80_RS21100 tRNA‑Gln/tRNA‑Gln
RA NZ_CP022686 4,270,799 A→C 9.0% T482P (ACG→CCG)  sucA → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 4,287,456 T→G 11.7% noncoding (47/76 nt) CIW80_RS21475 → tRNA‑Val
RA NZ_CP022686 4,291,808 T→G 8.1% G178G (GGT→GGG aroG → 3‑deoxy‑7‑phosphoheptulonate synthase AroG
RA NZ_CP022686 4,331,905 A→C 10.5% G300G (GGT→GGG ybhR ← ABC transporter permease
RA NZ_CP022686 4,338,911 A→C 10.3% K448N (AAA→AAC rhlE → ATP‑dependent RNA helicase RhlE
RA NZ_CP022686 4,427,623 G→T 7.5% intergenic (+88/‑11) yljB → / → clpS protein YljB/ATP‑dependent Clp protease adapter ClpS
RA NZ_CP022686 4,632,182 T→G 8.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,186 G→T 5.1% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 8.8% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,661,560 C→A 6.8% intergenic (+5/‑178) CIW80_RS26570 → / → CIW80_RS23365 hypothetical protein/tRNA‑Arg
RA NZ_CP022686 4,678,041 A→C 21.4% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,764 G→C 14.3% A52G (GCT→GGT)  yceB ← lipoprotein
RA NZ_CP022686 4,757,527 T→G 8.5% G91G (GGT→GGG ycfJ → glycine zipper 2TM domain‑containing protein
RA NZ_CP022686 4,764,880 T→G 12.8% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 13.7% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,815,879 T→G 11.4% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,916,128 A→C 6.7% intergenic (+255/+30) tdk → / ← CIW80_RS24885 thymidine kinase/IS4 family transposase
RA NZ_CP022686 4,919,017 T→G 9.3% E330D (GAA→GAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 5,043,855 T→A 14.6% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,951 T→C 6.9% G1194G (GGT→GGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 302–2618 3082–2957 340–2781 10495 [10394] [10202] 10542 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4746–4866 5478–5074 209–733 5080 [4950] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =0 (0.000)200 (1.010) 65/250 NT 100% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624680 (0.000)205 (1.030) 66/250 NT 100% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase