breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_CP022686 | 37,785 | T→G | 9.9% | K3N (AAA→AAC) | ansP ← | L‑asparagine permease |
RA | NZ_CP022686 | 360,227 | T→G | 22.1% | T35P (ACG→CCG) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | NZ_CP022686 | 372,574 | A→C | 18.1% | N275T (AAC→ACC) | sdaA → | L‑serine ammonia‑lyase |
RA | NZ_CP022686 | 457,894 | Δ1 bp | 100% | pseudogene (288/997 nt) | CIW80_RS02445 ← | DUF968 domain‑containing protein |
RA | NZ_CP022686 | 556,940 | A→T | 13.5% | intergenic (+124/‑724) | zinT → / → CIW80_RS03080 | metal‑binding protein ZinT/site‑specific integrase |
RA | NZ_CP022686 | 560,880 | C→T | 100% | intergenic (+62/+53) | CIW80_RS03085 → / ← pilV | hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV |
RA | NZ_CP022686 | 601,295 | T→G | 24.1% | V1607G (GTC→GGC) | irp1 → | yersiniabactin polyketide synthase HMWP1 |
MC JC | NZ_CP022686 | 628,478 | Δ1,286 bp | 100% | [clbP] | [clbP] | |
RA | NZ_CP022686 | 741,905 | A→C | 10.8% | E261D (GAA→GAC) | hisD → | histidinol dehydrogenase |
RA | NZ_CP022686 | 756,115 | A→G | 100% | G181G (GGT→GGC) | galE ← | UDP‑glucose 4‑epimerase GalE |
RA | NZ_CP022686 | 764,997 | T→G | 14.9% | N41T (AAC→ACC) | wcaM ← | colanic acid biosynthesis protein WcaM |
RA | NZ_CP022686 | 794,460 | C→T | 100% | A568V (GCG→GTG) | dgcE → | diguanylate cyclase |
RA | NZ_CP022686 | 829,452 | G→T | 21.4% | intergenic (‑41/+186) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 829,472 | G→T | 15.3% | intergenic (‑61/+166) | gatY ← / ← fbaB | tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase |
RA | NZ_CP022686 | 917,427:1 | +TT | 100% | intergenic (+130/‑91) | yeiP → / → yeiQ | elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein |
RA | NZ_CP022686 | 957,112 | T→G | 11.9% | T89P (ACC→CCC) | ccmA ← | cytochrome c biogenesis heme‑transporting ATPase CcmA |
RA | NZ_CP022686 | 1,000,401 | A→C | 10.2% | V955G (GTG→GGG) | yfaL ← | AIDA‑I family autotransporter adhesin YfaL/EhaC |
RA | NZ_CP022686 | 1,065,997 | A→C | 10.9% | A30A (GCT→GCG) | yfbS ← | SLC13 family permease |
RA | NZ_CP022686 | 1,209,908 | T→G | 19.8% | D396A (GAC→GCC) | eutH ← | ethanolamine utilization protein EutH |
RA | NZ_CP022686 | 1,237,918 | C→A | 10.3% | C303F (TGC→TTC) | tmcA ← | tRNA cytosine(34) acetyltransferase TmcA |
RA | NZ_CP022686 | 1,280,117 | T→G | 8.3% | N8H (AAC→CAC) | yfgM ← | ancillary SecYEG translocon subunit YfgM |
RA | NZ_CP022686 | 1,353,233 | A→C | 13.8% | V72G (GTT→GGT) | yfiF ← | tRNA/rRNA methyltransferase |
RA | NZ_CP022686 | 1,360,724 | G→T | 8.3% | intergenic (‑59/+265) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,360,742 | C→A | 8.5% | intergenic (‑77/+247) | kgtP ← / ← rrf | alpha‑ketoglutarate permease/5S ribosomal RNA |
RA | NZ_CP022686 | 1,396,420 | T→G | 7.3% | intergenic (+26/‑211) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,396,426 | T→G | 7.5% | intergenic (+32/‑205) | gabT → / → gabP | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease |
RA | NZ_CP022686 | 1,424,342 | C→G | 10.9% | intergenic (‑38/+25) | CIW80_RS07085 ← / ← CIW80_RS07090 | tRNA‑Arg/tRNA‑Arg |
RA | NZ_CP022686 | 1,443,501 | A→C | 10.1% | V20G (GTC→GGC) | hypF ← | carbamoyltransferase HypF |
RA | NZ_CP022686 | 1,465,000 | G→T | 12.6% | E173* (GAA→TAA) | pphB → | protein‑serine/threonine phosphatase |
RA | NZ_CP022686 | 1,479,607 | A→C | 16.9% | intergenic (‑16/+177) | ftsB ← / ← ygbE | cell division protein FtsB/DUF3561 family protein |
RA | NZ_CP022686 | 1,504,355 | T→A | 9.2% | A264A (GCT→GCA) | CIW80_RS07520 → | YgcG family protein |
RA | NZ_CP022686 | 1,535,353 | G→T | 32.9% | M31I (ATG→ATT) | fucI → | L‑fucose isomerase |
RA | NZ_CP022686 | 1,535,365 | A→C | 31.0% | K35N (AAA→AAC) | fucI → | L‑fucose isomerase |
RA | NZ_CP022686 | 1,547,118 | G→C | 12.7% | intergenic (+30/‑4) | CIW80_RS07710 → / → CIW80_RS07715 | tRNA‑Met/tRNA‑Met |
RA | NZ_CP022686 | 1,558,391 | T→A | 9.6% | V463E (GTA→GAA) | vgrG → | type VI secretion system Vgr family protein |
RA | NZ_CP022686 | 1,558,395 | A→C | 10.9% | K464N (AAA→AAC) | vgrG → | type VI secretion system Vgr family protein |
RA | NZ_CP022686 | 1,597,235 | T→G | 12.3% | N19H (AAC→CAC) | thyA ← | thymidylate synthase |
RA | NZ_CP022686 | 1,633,846 | T→G | 32.1% | N253T (AAC→ACC) | yqeB ← | selenium‑dependent molybdenum cofactor biosynthesis protein YqeB |
RA | NZ_CP022686 | 1,640,888 | G→T | 12.1% | intergenic (+34/‑17) | ssnA → / → ygfM | putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
RA | NZ_CP022686 | 1,674,224 | T→G | 18.9% | K188N (AAA→AAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,231 | A→T | 14.7% | V186E (GTA→GAA) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,244 | T→G | 12.5% | T182P (ACC→CCC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,248 | T→G | 18.1% | Q180H (CAA→CAC) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,674,260 | A→T | 7.2% | F176L (TTT→TTA) | ygfB ← | UPF0149 family protein YgfB |
RA | NZ_CP022686 | 1,705,975 | A→T | 12.7% | intergenic (‑585/‑70) | yqgB ← / → metK | acid stress response protein YqgB/methionine adenosyltransferase |
RA | NZ_CP022686 | 1,710,453 | A→C | 8.1% | intergenic (+48/‑32) | endA → / → rsmE | deoxyribonuclease I/16S rRNA (uracil(1498)‑N(3))‑methyltransferase |
RA | NZ_CP022686 | 1,716,167 | A→C | 8.3% | N49T (AAC→ACC) | rdgB → | XTP/dITP diphosphatase |
RA | NZ_CP022686 | 1,860,070 | C→A | 12.2% | E119* (GAA→TAA) | CIW80_RS09255 ← | YtfJ family protein |
RA | NZ_CP022686 | 1,860,077 | G→T | 11.6% | G116G (GGC→GGA) | CIW80_RS09255 ← | YtfJ family protein |
RA | NZ_CP022686 | 1,911,602 | T→G | 9.0% | N224T (AAC→ACC) | CIW80_RS09520 ← | MurR/RpiR family transcriptional regulator |
RA | NZ_CP022686 | 1,954,923 | T→G | 25.8% | T92P (ACG→CCG) | ttdR ← | DNA‑binding transcriptional activator TtdR |
RA | NZ_CP022686 | 2,049,089 | T→G | 16.0% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,049,112 | C→A | 10.5% | A248A (GCG→GCT) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | NZ_CP022686 | 2,076,588 | C→A | 9.0% | intergenic (+58/‑175) | ispB → / → sfsB | octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB |
RA | NZ_CP022686 | 2,148,115 | T→G | 16.6% | L126R (CTG→CGG) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,148,136 | T→G | 9.0% | V133G (GTT→GGT) | panF → | sodium/pantothenate symporter |
RA | NZ_CP022686 | 2,159,673 | C→A | 14.3% | A167E (GCA→GAA) | CIW80_RS10780 → | amino acid ABC transporter substrate‑binding protein |
RA | NZ_CP022686 | 2,159,687 | T→A | 8.7% | Y172N (TAC→AAC) | CIW80_RS10780 → | amino acid ABC transporter substrate‑binding protein |
RA | NZ_CP022686 | 2,172,177 | A→C | 7.8% | G87G (GGT→GGG) | smg ← | DUF494 family protein Smg |
RA | NZ_CP022686 | 2,185,071 | A→C | 6.4% | G47G (GGT→GGG) | rpsE ← | 30S ribosomal protein S5 |
RA | NZ_CP022686 | 2,185,075 | A→C | 9.3% | V46G (GTT→GGT) | rpsE ← | 30S ribosomal protein S5 |
RA | NZ_CP022686 | 2,188,239 | G→T | 10.8% | intergenic (‑108/+57) | rplN ← / ← rpsQ | 50S ribosomal protein L14/30S ribosomal protein S17 |
RA | NZ_CP022686 | 2,214,419 | T→C | 100% | K43R (AAA→AGA) | rpsL ← | 30S ribosomal protein S12 |
RA | NZ_CP022686 | 2,272,797 | G→T | 12.0% | intergenic (+39/‑58) | greB → / → yhgF | transcription elongation factor GreB/Tex family protein |
RA | NZ_CP022686 | 2,305,921 | G→T | 8.0% | P239T (CCT→ACT) | CIW80_RS11625 ← | fimbrial biogenesis usher protein |
RA | NZ_CP022686 | 2,320,775 | C→T | 100% | G274S (GGC→AGC) | CIW80_RS11675 ← | DNA‑processing protein DprA |
RA | NZ_CP022686 | 2,357,291 | A→C | 13.3% | N26T (AAC→ACC) | dcrB → | phage sensitivity protein DcrB |
RA | NZ_CP022686 | 2,383,173 | T→G | 9.0% | intergenic (+64/+50) | uspA → / ← CIW80_RS26230 | universal stress protein UspA/hypothetical protein |
RA | NZ_CP022686 | 2,397,370 | T→G | 12.3% | F9C (TTT→TGT) | chuT → | heme ABC transporter substrate‑binding protein ChuT |
RA | NZ_CP022686 | 2,408,861 | Δ1 bp | 100% | pseudogene (1097/3115 nt) | mdtF → | multidrug efflux pump RND permease MdtF |
RA | NZ_CP022686 | 2,470,422 | T→G | 15.7% | K72N (AAA→AAC) | glyS ← | glycine‑‑tRNA ligase subunit beta |
RA | NZ_CP022686 | 2,470,427 | C→G | 7.5% | E71Q (GAA→CAA) | glyS ← | glycine‑‑tRNA ligase subunit beta |
RA | NZ_CP022686 | 2,517,086 | A→C | 5.7% | K832N (AAA→AAC) | upaG → | trimeric autotransporter adhesin UpaG |
RA | NZ_CP022686 | 2,558,874 | T→G | 6.2% | V131G (GTT→GGT) | yicG → | trimeric intracellular cation channel family protein |
RA | NZ_CP022686 | 2,559,398 | T→G | 8.5% | N461T (AAC→ACC) | ligB ← | NAD‑dependent DNA ligase LigB |
RA | NZ_CP022686 | 2,572,721 | A→C | 11.5% | F186C (TTT→TGT) | CIW80_RS12885 ← | ROK family protein |
RA | NZ_CP022686 | 2,574,429 | T→G | 14.8% | N230T (AAC→ACC) | CIW80_RS12895 ← | class II fructose‑bisphosphate aldolase family protein |
JC | NZ_CP022686 | 2,590,563 | +TTGG | 100% | coding (109/153 nt) | CIW80_RS26455 → | hypothetical protein |
RA | NZ_CP022686 | 2,717,385 | T→G | 7.2% | F96V (TTT→GTT) | ilvA → | threonine ammonia‑lyase, biosynthetic |
RA | NZ_CP022686 | 2,733,571 | T→G | 15.1% | F53V (TTT→GTT) | rffG → | dTDP‑glucose 4,6‑dehydratase |
RA | NZ_CP022686 | 2,750,805 | T→G | 8.5% | N297T (AAC→ACC) | hemC ← | hydroxymethylbilane synthase |
RA | NZ_CP022686 | 2,758,153 | A→C | 9.6% | T49P (ACG→CCG) | yifL → | lipoprotein |
RA | NZ_CP022686 | 2,868,740 | T→G | 22.7% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,868,753 | T→G | 15.4% | T18P (ACC→CCC) | fdoH ← | formate dehydrogenase O subunit beta |
RA | NZ_CP022686 | 2,904,856 | T→G | 12.6% | K237N (AAA→AAC) | ftsN ← | cell division protein FtsN |
RA | NZ_CP022686 | 2,920,418 | C→A | 8.1% | A65E (GCA→GAA) | CIW80_RS14610 → | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
RA | NZ_CP022686 | 2,989,627 | T→G | 13.3% | Q39H (CAA→CAC) | thiC ← | phosphomethylpyrimidine synthase ThiC |
RA | NZ_CP022686 | 3,184,651 | A→C | 7.7% | intergenic (‑98/+18) | cutA ← / ← dcuA | divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA |
JC | NZ_CP022686 | 3,196,873 | +GCGTAATCGGTT | 100% | coding (944/1122 nt) | ampC ← | BlaEC family class C beta‑lactamase |
RA | NZ_CP022686 | 3,217,502 | A→C | 6.5% | T594P (ACG→CCG) | mutL → | DNA mismatch repair endonuclease MutL |
RA | NZ_CP022686 | 3,218,260 | T→G | 8.9% | L233R (CTT→CGT) | miaA → | tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA |
RA | NZ_CP022686 | 3,252,198 | C→A | 14.3% | E219* (GAA→TAA) | CIW80_RS16225 ← | enoyl‑CoA hydratase/isomerase family protein |
RA | NZ_CP022686 | 3,265,096 | G→T | 12.1% | A73E (GCA→GAA) | ytfJ ← | YtfJ family protein |
RA | NZ_CP022686 | 3,281,055 | C→G | 10.7% | P92A (CCG→GCG) | yjfF → | sugar ABC transporter permease YjfF |
RA | NZ_CP022686 | 3,297,360 | T→G | 10.4% | L837V (TTA→GTA) | mgtA → | magnesium‑translocating P‑type ATPase |
RA | NZ_CP022686 | 3,348,357 | T→G | 6.9% | intergenic (‑173/‑27) | CIW80_RS16685 ← / → CIW80_RS26495 | inovirus Gp2 family protein/hypothetical protein |
RA | NZ_CP022686 | 3,390,625 | C→A | 14.0% | D140Y (GAT→TAT) | sgcX ← | M42 family metallopeptidase |
RA | NZ_CP022686 | 3,395,243 | G→T | 22.8% | pseudogene (13/1107 nt) | yjhR → | helicase YjhR |
RA | NZ_CP022686 | 3,424,795 | A→C | 100% | intergenic (‑41/+27) | yjiJ ← / ← yjiK | MFS transporter/YjiK family protein |
RA | NZ_CP022686 | 3,444,704 | T→G | 13.9% | T530P (ACC→CCC) | btsT ← | pyruvate/proton symporter BtsT |
RA | NZ_CP022686 | 3,459,409 | C→A | 7.4% | S75Y (TCT→TAT) | bglJ → | DNA‑binding transcriptional activator BglJ |
RA | NZ_CP022686 | 3,461,229 | T→G | 16.8% | noncoding (84/87 nt) | CIW80_RS17340 ← | tRNA‑Leu |
RA | NZ_CP022686 | 3,552,879 | G→T | 5.4% | Q677K (CAA→AAA) | lptD ← | LPS assembly protein LptD |
MC JC | NZ_CP022686 | 3,559,837 | Δ2,343 bp | 100% | coding (10‑2352/2352 nt) | polB ← | DNA polymerase II |
RA | NZ_CP022686 | 3,656,188 | A→G | 100% | S549P (TCC→CCC) | htrE ← | outer membrane usher protein |
RA | NZ_CP022686 | 3,734,367 | T→G | 24.8% | F225V (TTT→GTT) | yafD → | endonuclease/exonuclease/phosphatase family protein |
RA | NZ_CP022686 | 3,792,805 | A→G | 100% | Y421C (TAC→TGC) | fucP → | L‑fucose:H+ symporter permease |
MC JC | NZ_CP022686 | 3,844,426 | Δ1,036 bp | 100% | coding (2‑1037/1056 nt) | dinB → | DNA polymerase IV |
RA | NZ_CP022686 | 3,885,457 | A→C | 9.1% | N1404T (AAC→ACC) | fdeC → | inverse autotransporter adhesin FdeC |
RA | NZ_CP022686 | 3,909,238 | T→G | 6.1% | D280E (GAT→GAG) | pdeL → | DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL |
RA | NZ_CP022686 | 3,925,304 | A→C | 12.6% | Q161P (CAG→CCG) | prpC → | 2‑methylcitrate synthase |
RA | NZ_CP022686 | 3,932,443 | C→A | 18.9% | E184* (GAA→TAA) | lacA ← | galactoside O‑acetyltransferase |
RA | NZ_CP022686 | 3,935,324 | T→G | 11.4% | N705T (AAC→ACC) | lacZ ← | beta‑galactosidase |
RA | NZ_CP022686 | 3,940,707 | A→C | 8.1% | F314V (TTT→GTT) | frmA ← | S‑(hydroxymethyl)glutathione dehydrogenase |
RA | NZ_CP022686 | 3,977,656 | A→C | 7.5% | K88N (AAA→AAC) | phoR → | phosphate regulon sensor histidine kinase PhoR |
RA | NZ_CP022686 | 4,014,225 | C→G | 8.6% | intergenic (+71/‑273) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,238 | C→G | 11.8% | intergenic (+84/‑260) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,014,242 | A→T | 12.9% | intergenic (+88/‑256) | bolA → / → tig | transcriptional regulator BolA/trigger factor |
RA | NZ_CP022686 | 4,025,380 | G→T | 5.9% | T437K (ACA→AAA) | ybaE ← | SgrR family transcriptional regulator |
RA | NZ_CP022686 | 4,032,257 | G→T | 14.2% | E95* (GAA→TAA) | glnK → | P‑II family nitrogen regulator |
RA | NZ_CP022686 | 4,056,962 | C→A | 17.9% | F137L (TTC→TTA) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,056,975 | G→T | 17.0% | V142L (GTA→TTA) | adk → | adenylate kinase |
RA | NZ_CP022686 | 4,079,329 | A→C | 11.7% | S170A (TCA→GCA) | ybbO ← | NADP(+)‑dependent aldehyde reductase |
RA | NZ_CP022686 | 4,188,944 | A→C | 7.5% | V321G (GTA→GGA) | rlpA ← | endolytic peptidoglycan transglycosylase RlpA |
RA | NZ_CP022686 | 4,196,276 | T→G | 13.1% | E15D (GAA→GAC) | holA ← | DNA polymerase III subunit delta |
RA | NZ_CP022686 | 4,331,905 | A→C | 9.8% | G300G (GGT→GGG) | ybhR ← | ABC transporter permease |
RA | NZ_CP022686 | 4,331,909 | G→T | 12.8% | S299Y (TCC→TAC) | ybhR ← | ABC transporter permease |
RA | NZ_CP022686 | 4,368,341 | T→G | 13.7% | D47A (GAC→GCC) | ybiY ← | glycyl‑radical enzyme activating protein |
RA | NZ_CP022686 | 4,419,919 | C→G | 62.6% | intergenic (‑340/+154) | lysO ← / ← aqpZ | L‑lysine exporter LysO/aquaporin Z |
RA | NZ_CP022686 | 4,440,334 | A→C | 17.4% | Q797H (CAA→CAC) | ftsK → | DNA translocase FtsK |
RA | NZ_CP022686 | 4,461,754 | A→T | 5.8% | intergenic (+108/‑62) | aroA → / → ycaL | 3‑phosphoshikimate 1‑carboxyvinyltransferase/metallopeptidase YcaL |
RA | NZ_CP022686 | 4,535,349 | A→C | 10.6% | T118T (ACA→ACC) | appA → | bifunctional acid phosphatase/4‑phytase |
RA | NZ_CP022686 | 4,632,182 | T→G | 11.1% | R276R (CGT→CGG) | mchF → | microcin export transporter peptidase/ATP‑binding subunit MchF |
RA | NZ_CP022686 | 4,678,047 | A→C | 35.8% | intergenic (+45/‑76) | agn43 → / → CIW80_RS23450 | autotransporter adhesin Ag43/hypothetical protein |
RA | NZ_CP022686 | 4,712,763 | A→C | 16.2% | A52A (GCT→GCG) | yceB ← | lipoprotein |
RA | NZ_CP022686 | 4,764,877 | T→G | 9.0% | intergenic (‑57/‑204) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,764,880 | T→G | 10.2% | intergenic (‑60/‑201) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,764,914 | C→A | 12.5% | intergenic (‑94/‑167) | ycfT ← / → lolC | acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC |
RA | NZ_CP022686 | 4,780,176 | A→C | 16.0% | intergenic (‑75/+95) | phoP ← / ← purB | two‑component system response regulator PhoP/adenylosuccinate lyase |
RA | NZ_CP022686 | 4,783,334 | A→C | 16.4% | V32G (GTC→GGC) | mnmA ← | tRNA 2‑thiouridine(34) synthase MnmA |
MC JC | NZ_CP022686 | 4,844,868 | Δ1,662 bp | 100% | [umuD]–[umuC] | [umuD], [umuC] | |
RA | NZ_CP022686 | 4,870,912 | Δ1 bp | 100% | intergenic (‑108/‑120) | dhaK ← / → dhaR | dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR |
RA | NZ_CP022686 | 4,919,022 | T→G | 8.0% | S329R (AGC→CGC) | adhE ← | bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
RA | NZ_CP022686 | 4,951,538 | C→A | 21.4% | A44A (GCC→GCA) | topA → | type I DNA topoisomerase |
RA | NZ_CP022686 | 4,980,895 | A→C | 8.8% | C101W (TGT→TGG) | sapD ← | peptide ABC transporter ATP‑binding protein SapD |
RA | NZ_CP022686 | 5,043,402 | G→A | 5.1% | V1011V (GTG→GTA) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | NZ_CP022686 | 5,043,855 | T→C | 9.0% | G1162G (GGT→GGC) | CIW80_RS25525 → | EntS/YbdA MFS transporter |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 2,031 | G→A | 100% | intergenic (‑235/‑1500) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,485 | A→C | 24.1% | intergenic (‑1689/‑46) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,516 | Δ11 bp | 16.7% | intergenic (‑1720/‑5) | putative rom (RNA I inhibition modulator protein) ← / → ompA | –/– |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,591 | Δ25 bp | 54.1% | [ompA]–[IL2wt no signal] | [ompA], [IL2wt no signal] | |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,595 | Δ15 bp | 100% | [IL2wt no signal] | [IL2wt no signal] | |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,655 | A→T | 5.1% | H16L (CAT→CTT) | IL2wt no signal → | |
RA | pMut‑Ompa‑D5‑IL2wt‑His6 | 3,760 | Δ1 bp | 45.3% | coding (152/399 nt) | IL2wt no signal → | |
JC | pMut‑Ompa‑D5‑IL2wt‑His6 | 4,019 | +CAT | 8.1% | intergenic (+12/+37) | IL2wt no signal → / ← annotated as hok_sok locus | –/– |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_MW240712 | 303–2618 | 3084–2957 | 340–2782 | 12522 [12344] | [12054] 13083 | [JNG15_RS00005]–mobA | [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA |
* | * | ÷ | pMut‑Ompa‑D5‑IL2wt‑His6 | 4746–4809 | 5478–5074 | 266–733 | 5958 [5752] | [0] NA | [putative HTH domain protein] | [putative HTH domain protein] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP022686 | 262466 = | 1 (0.010) | 164 (0.920) | 70/250 | NT | 99.4% | coding (320/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1524130 | NA (NA) | intergenic (+8/+232) | CIW80_RS07590/syd | IS3‑like element ISEc52 family transposase/SecY‑interacting protein | |||||
* | ? | NZ_CP022686 | = 262468 | 1 (0.010) | 157 (0.890) | 67/250 | NT | 99.4% | coding (322/1647 nt) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
? | NZ_CP022686 | = 1761697 | NA (NA) | intergenic (‑72/‑70) | CIW80_RS08710/CIW80_RS08720 | IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase | |||||
* | ? | NZ_CP022686 | = 3395245 | 184 (1.040) | 68 (0.030) +61 bp |
35/128 | NT | 58.1% | pseudogene (15/1107 nt) | yjhR | helicase YjhR |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | = 3594 | 9 (0.000) | intergenic (+1/‑15) | ompA/IL2wt no signal | –/– | |||||
* | ? | NZ_CP022686 | 3899666 = | 201 (1.130) | 33 (0.210) | 21/218 | NT | 16.1% | intergenic (‑40/+290) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | 3899944 = | 168 (1.090) | intergenic (‑318/+12) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
* | ? | NZ_CP022686 | = 3899681 | 195 (1.100) | 26 (0.170) | 21/218 | NT | 13.3% | intergenic (‑55/+275) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA |
? | NZ_CP022686 | = 3899927 | 168 (1.090) | intergenic (‑301/+29) | hyxR/ipbA | LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA | |||||
* | ? | NZ_CP022686 | 4514374 = | 156 (0.880) | 10 (0.000) +AGCT |
9/242 | NT | 11.1% | coding (59/1053 nt) | ompA | porin OmpA |
? | pMut‑Ompa‑D5‑IL2wt‑His6 | 3609 = | 9 (0.000) | coding (1/399 nt) | IL2wt no signal |