breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA NZ_CP022686 457,895 Δ1 bp pseudogene (287/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
MC JC NZ_CP022686 628,478 Δ1,286 bp [clbP] [clbP]
RA NZ_CP022686 756,115 A→G G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 917,427 +TT intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 2,214,419 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,320,775 C→T G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,408,862 Δ1 bp pseudogene (1098/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
JC NZ_CP022686 2,590,564 +TGGT coding (110/153 nt) CIW80_RS26455 → hypothetical protein
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,424,795 A→C intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
MC JC NZ_CP022686 3,559,837 Δ2,343 bp coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,656,188 A→G S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,792,805 A→G Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 4,419,926 C→G intergenic (‑347/+147) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
MC JC NZ_CP022686 4,844,868 Δ1,662 bp [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,914 Δ1 bp intergenic (‑110/‑118) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA pMUT1‑hok‑empty 4,123 +T intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 303 3084–2957 2655–2782 2971 [2931] [2875] 3190 [JNG15_RS00005]–mobA [JNG15_RS00005],JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1256–1317 3987–1722 406–2732 1376 [1327] [1264] 1578 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =4 (0.020)128 (0.790) 54/270 0.5 97.0% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624682 (0.010)92 (0.570) 40/270 1.0 97.9% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? pMUT1‑hok‑empty 456 =1062 (0.480)1482 (0.670) 149/270 0.9 66.4% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 439 (0.200)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4911805 (0.820)1452 (0.810) 122/220 0.9 60.4% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 435 (0.240)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–