breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 177,514 T→G 7.4% T138P (ACC→CCC)  uidB ← glucuronide transporter
RA NZ_CP022686 235,846 A→C 6.0% N287T (AAC→ACC)  pykF → pyruvate kinase PykF
RA NZ_CP022686 246,371 T→G 7.1% N535T (AAC→ACC)  ydiJ ← FAD‑binding and (Fe‑S)‑binding domain‑containing protein
RA NZ_CP022686 298,027 A→C 15.7% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 340,516 A→C 9.4% F31V (TTT→GTT)  ydjK ← MFS transporter
RA NZ_CP022686 360,227 T→G 12.3% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 360,246 T→G 11.1% E28D (GAA→GAC yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 16.0% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 402,874 A→C 7.5% pseudogene (785/2061 nt) ptrB ← oligopeptidase B
RA NZ_CP022686 436,575 T→A 7.6% intergenic (‑3/+54) CIW80_RS02305 ← / ← CIW80_RS02310 phage minor tail protein L/phage tail protein
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 481,052 T→G 7.6% T604T (ACA→ACC flhA ← flagellar biosynthesis protein FlhA
RA NZ_CP022686 496,128 G→C 10.1% L385V (CTT→GTT)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 531,408 A→C 16.4% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 606,995 A→C 9.5% R344S (AGA→AGC ybtU → yersiniabactin biosynthesis oxidoreductase YbtU
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 11.7% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 694,421 G→T 7.1% intergenic (+181/‑15) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 13.5% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,001 A→T 10.6% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 828,846 A→C 10.4% L189R (CTT→CGT)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA NZ_CP022686 829,441 C→A 30.5% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 17.9% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 17.6% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,555 T→G 14.8% intergenic (‑128/+95) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 881,745 C→G 10.9% intergenic (+260/+127) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,035 A→T 10.1% intergenic (+104/‑153) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 17.2% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 17.8% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,000,401 A→C 16.7% V955G (GTG→GGG)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,073 A→C 8.1% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,032,777 A→C 9.6% V278G (GTG→GGG)  menE ← o‑succinylbenzoate‑‑CoA ligase
RA NZ_CP022686 1,040,878 T→G 9.5% L222R (CTG→CGG)  rbn → ribonuclease BN
RA NZ_CP022686 1,040,909 A→C 10.0% E232D (GAA→GAC rbn → ribonuclease BN
JC NZ_CP022686 1,061,329 Δ6 bp 10.1% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,065,997 A→C 14.5% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,944 T→G 9.3% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,195,297 C→A 8.4% K132N (AAG→AAT cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA NZ_CP022686 1,209,908 T→G 19.3% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,213,479 T→G 13.7% G420G (GGA→GGC eutE ← aldehyde dehydrogenase EutE
RA NZ_CP022686 1,349,193 A→C 10.3% K401N (AAA→AAC srmB → ATP‑dependent RNA helicase SrmB
RA NZ_CP022686 1,360,724 G→T 14.9% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 15.2% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,366,270 T→G 8.9% intergenic (‑271/+171) CIW80_RS06740 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA NZ_CP022686 1,370,556 T→G 18.4% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,619 T→G 8.9% E78D (GAA→GAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,396,420 T→G 6.5% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,434,530 A→C 6.1% T253P (ACC→CCC)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA NZ_CP022686 1,447,198 T→A 8.7% V35V (GTA→GTT hycI ← hydrogenase maturation peptidase HycI
RA NZ_CP022686 1,482,223 T→A 25.6% M293L (ATG→TTG)  cysD ← sulfate adenylyltransferase subunit CysD
RA NZ_CP022686 1,504,289 T→G 12.3% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,323 C→A 14.7% P254T (CCT→ACT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,328 G→T 15.3% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 15.1% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,535,365 A→C 36.4% K35N (AAA→AAC fucI → L‑fucose isomerase
RA NZ_CP022686 1,564,589 A→C 11.0% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,566,271 A→C 10.5% T758P (ACC→CCC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,579,312 A→C 12.6% V107V (GTT→GTG CIW80_RS07835 ← PRD domain‑containing protein
RA NZ_CP022686 1,619,230 C→A 5.5% intergenic (+19/+298) yqeG → / ← CIW80_RS07990 amino acid permease/tRNA‑Gly
RA NZ_CP022686 1,632,480 T→G 8.8% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 25.7% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,633,901 T→G 9.2% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 18.4% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 15.4% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,706,032 G→T 9.4% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,202 A→C 11.2% T61P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,758,771 T→G 7.8% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,798,978 A→T 5.2% Y9F (TAT→TTT)  CIW80_RS08965 → DUF932 domain‑containing protein
RA NZ_CP022686 1,865,103 A→C 8.3% L491V (TTG→GTG)  pitB ← inorganic phosphate transporter PitB
RA NZ_CP022686 1,911,602 T→G 13.0% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,925,332 A→C 10.8% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 1,954,923 T→G 14.3% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 8.4% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 6.7% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,035,557 G→T 7.3% I126I (ATC→ATA rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA NZ_CP022686 2,047,987 A→C 5.8% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 15.3% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 9.9% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,112 C→A 6.8% A248A (GCG→GCT deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,102 A→C 8.7% A636A (GCT→GCG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,061,785 A→C 8.4% E385A (GAG→GCG)  argG → argininosuccinate synthase
RA NZ_CP022686 2,076,554 T→G 11.2% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 13.6% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,461 A→T 7.5% V11E (GTA→GAA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 10.1% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,122 T→G 7.0% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 10.0% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,177 A→C 8.7% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,188,239 G→T 7.5% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,215,205 G→C 5.1% A41G (GCT→GGT)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,215,215 A→C 7.4% F38V (TTC→GTC)  tusC ← sulfurtransferase complex subunit TusC
RA NZ_CP022686 2,272,797 G→T 7.5% intergenic (+39/‑58) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,340,264 A→C 8.3% V91G (GTC→GGC)  livF ← high‑affinity branched‑chain amino acid ABC transporter ATP‑binding protein LivF
RA NZ_CP022686 2,356,231 T→G 7.4% D9A (GAC→GCC)  tusA ← sulfurtransferase TusA
RA NZ_CP022686 2,357,291 A→C 13.6% N26T (AAC→ACC)  dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,357,322 T→A 19.0% S36R (AGT→AGA dcrB → phage sensitivity protein DcrB
RA NZ_CP022686 2,394,287 T→G 22.1% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,394,302 A→T 11.4% F110Y (TTC→TAC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,398,896 A→C 11.1% K206N (AAA→AAC hutW → heme anaerobic degradation radical SAM methyltransferase ChuW/HutW
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,535 A→C 8.8% L15R (CTT→CGT)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 21.5% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,572,705 A→C 9.9% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 11.4% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 7.8% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,701,400 G→T 5.9% R328S (CGC→AGC)  mdtD ← multidrug transporter subunit MdtD
RA NZ_CP022686 2,719,177 A→C 11.2% S119A (TCG→GCG)  ilvY ← HTH‑type transcriptional activator IlvY
RA NZ_CP022686 2,733,571 T→G 8.0% F53V (TTT→GTT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA NZ_CP022686 2,736,585 T→A 8.7% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 16.9% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 10.0% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,601 G→T 16.3% intergenic (+16/‑27) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,758,128 A→C 8.5% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 9.9% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 8.2% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,769,178 A→C 11.1% L53R (CTG→CGG)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,810,040 A→C 8.4% N242T (AAC→ACC)  tatC → Sec‑independent protein translocase subunit TatC
RA NZ_CP022686 2,821,644 A→C 8.7% intergenic (+100/‑278) hemG → / → CIW80_RS14130 menaquinone‑dependent protoporphyrinogen IX dehydrogenase/16S ribosomal RNA
RA NZ_CP022686 2,868,740 T→G 18.7% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 9.3% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 9.2% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.2% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 9.2% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 7.0% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 7.3% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,928,566 G→T 6.4% Q6K (CAA→AAA)  gldA ← bifunctional L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA NZ_CP022686 2,977,481 T→G 6.4% V558G (GTA→GGA)  rpoB → DNA‑directed RNA polymerase subunit beta
RA NZ_CP022686 2,981,612 A→C 9.7% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 3,017,622 A→C 19.7% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 9.0% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,955 C→A 14.6% S43* (TCA→TAA)  CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,114,653 A→C 8.6% V36G (GTC→GGC)  CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,136,935 T→G 10.1% S163R (AGC→CGC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,940 T→G 10.3% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,252 T→G 11.9% F20V (TTT→GTT)  rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,155,195 T→G 5.1% T411P (ACC→CCC)  eptA ← phosphoethanolamine transferase EptA
RA NZ_CP022686 3,184,651 A→C 8.8% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,200,595 A→C 7.1% V199G (GTT→GGT)  frdA ← fumarate reductase (quinol) flavoprotein subunit
RA NZ_CP022686 3,265,084 T→G 14.7% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,265,096 G→T 8.9% A73E (GCA→GAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,265,118 G→T 11.3% H66N (CAT→AAT)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,297,333 T→G 16.7% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,334,297 A→C 7.7% F248V (TTT→GTT)  fecD ← Fe(3+) dicitrate ABC transporter permease subunit FecD
RA NZ_CP022686 3,348,342 A→T 6.4% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 7.3% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 8.1% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,360 C→A 6.8% intergenic (‑176/‑24) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 6.2% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,363,082 G→T 10.6% intergenic (+781/‑10) CIW80_RS16815 → / → CIW80_RS16820 helix‑turn‑helix domain‑containing protein/Alw26I/Eco31I/Esp3I family type II restriction adenine‑specific DNA‑methyltransferase
RA NZ_CP022686 3,376,248 A→C 5.4% K152N (AAA→AAC mtnA → S‑methyl‑5‑thioribose‑1‑phosphate isomerase
RA NZ_CP022686 3,387,644 A→C 8.1% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,704 T→G 12.2% T530P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,215 A→T 12.5% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,218 G→C 14.2% intergenic (‑27/+8) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 14.7% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 10.4% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,270 T→A 7.7% noncoding (43/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,496,452 A→C 9.1% I273L (ATC→CTC)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,652,079 T→G 12.1% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 6.1% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,380 T→G 21.0% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 18.5% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 10.6% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,256 G→T 14.0% E1583* (GAA→TAA)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,806,265 T→A 10.5% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
RA NZ_CP022686 3,824,319 T→G 9.6% L213R (CTT→CGT)  CIW80_RS19155 → TolC family protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,885,457 A→C 9.4% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,895,534 T→G 9.6% intergenic (‑140/+44) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,910,255 G→T 9.3% F69L (TTC→TTA yahB ← LysR substrate‑binding domain‑containing protein
RA NZ_CP022686 3,928,345 T→G 10.1% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,369 T→G 8.6% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,932,443 C→A 15.8% E184* (GAA→TAA)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,935,324 T→G 11.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,724 A→C 7.6% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 4,014,238 C→G 14.2% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 8.5% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,250 C→G 9.5% intergenic (+96/‑248) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,032,284 A→C 12.4% T104P (ACC→CCC)  glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,951 C→A 14.2% H134N (CAC→AAC)  adk → adenylate kinase
RA NZ_CP022686 4,056,962 C→A 15.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,978 G→T 12.9% E143* (GAA→TAA)  adk → adenylate kinase
RA NZ_CP022686 4,074,874 A→C 5.7% R32R (CGT→CGG CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,074,899 A→C 6.1% V24G (GTG→GGG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,079,332 A→C 6.5% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,133,850 T→G 9.9% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,218,096 A→C 5.7% G319G (GGT→GGG nagC ← DNA‑binding transcriptional regulator NagC
RA NZ_CP022686 4,280,095 T→G 8.2% G103G (GGT→GGG cydB → cytochrome d ubiquinol oxidase subunit II
RA NZ_CP022686 4,286,455 T→G 11.7% V38G (GTC→GGC)  cpoB → cell division protein CpoB
RA NZ_CP022686 4,331,940 A→C 14.8% F289V (TTT→GTT)  ybhR ← ABC transporter permease
RA NZ_CP022686 4,368,341 T→G 10.8% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,368,380 T→G 14.8% N34T (AAC→ACC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,419,919 C→G 100% intergenic (‑340/+154) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
RA NZ_CP022686 4,419,926 C→G 100% intergenic (‑347/+147) lysO ← / ← aqpZ L‑lysine exporter LysO/aquaporin Z
RA NZ_CP022686 4,439,653 A→C 8.3% K570N (AAA→AAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→G 16.3% Q797Q (CAA→CAG ftsK → DNA translocase FtsK
RA NZ_CP022686 4,485,614 T→G 14.6% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,562,550 T→G 16.3% N140H (AAC→CAC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA NZ_CP022686 4,587,091 T→G 14.6% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,589,347 T→G 9.1% P253P (CCA→CCC CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,589,367 T→G 6.0% T247P (ACC→CCC)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,589,384 T→G 8.7% K241T (AAG→ACG)  CIW80_RS22920 ← PatB family C‑S lyase
RA NZ_CP022686 4,632,182 T→G 8.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,642,951 A→C 11.4% N732T (AAC→ACC)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,642,956 A→C 5.5% T734P (ACG→CCG)  sfaF → S/F1C fimbrial usher SfaF/FocD
RA NZ_CP022686 4,678,033 T→G 29.3% intergenic (+31/‑90) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,678,047 A→C 30.8% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,914 C→A 12.3% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,783,318 C→A 7.1% M37I (ATG→ATT mnmA ← tRNA 2‑thiouridine(34) synthase MnmA
RA NZ_CP022686 4,814,990 T→G 11.5% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,839,669 A→T 13.8% V150E (GTA→GAA)  minD ← septum site‑determining protein MinD
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,873 T→G 7.4% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 4,980,895 A→C 11.0% C101W (TGT→TGG sapD ← peptide ABC transporter ATP‑binding protein SapD
RA NZ_CP022686 5,041,191 G→A 5.4% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 7.0% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,831 C→A 9.0% D1154E (GAC→GAA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 1,326 A→C 11.2% intergenic (–/+391)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,336 C→A 14.9% intergenic (–/+381)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,375 Δ1 bp 8.5% intergenic (–/+342)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,376 Δ1 bp 89.4% intergenic (–/+341)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,423 G→T 10.9% intergenic (–/+294)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,430 G→T 17.2% intergenic (–/+287)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,441 G→T 7.7% intergenic (–/+276)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 1,450 G→T 8.1% intergenic (–/+267)  / ← putative HTH domain protein –/–
RA pMUT1‑hok‑empty 2,075:1 +G 14.7% intergenic (‑41/‑116) putative HTH domain protein ← / → uncharacterized protein –/–
RA pMUT1‑hok‑empty 2,078:1 +A 33.3% intergenic (‑44/‑113) putative HTH domain protein ← / → uncharacterized protein –/–
RA pMUT1‑hok‑empty 4,123:1 +T 93.4% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3084–2957 340–2896 15590 [14288] [14567] 15797 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =0 (0.000)218 (1.050) 86/250 NT 100% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624680 (0.000)194 (0.940) 79/250 NT 100% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =226 (1.090)45 (0.250) 26/218 NT 18.8% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 192 (1.060)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681222 (1.070)31 (0.170) 20/218 NT 13.9% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 192 (1.060)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =5009 (0.450)7024 (0.640) 228/250 NT 67.0% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1912 (0.170)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4917452 (0.670)5537 (0.630) 177/200 NT 58.4% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1918 (0.220)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–