breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 162,417 G→T 6.1% G50C (GGC→TGC)  ydgH → DUF1471 family protein YdgH
RA NZ_CP022686 197,828 C→A 13.2% F461L (TTC→TTA dtpA → dipeptide/tripeptide permease DtpA
RA NZ_CP022686 263,831 A→C 10.2% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 349,106 A→C 9.6% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 349,123 A→C 8.5% K612N (AAA→AAC yeaG → protein kinase YeaG
RA NZ_CP022686 349,997 C→A 11.2% A221A (GCC→GCA yeaH → YeaH/YhbH family protein
RA NZ_CP022686 360,227 T→G 20.1% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,563 T→A 12.5% V271V (GTT→GTA sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,116 C→A 8.9% A389S (GCG→TCG)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 21.2% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 16.8% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 651,848 A→C 12.7% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,052 T→G 6.7% T23P (ACC→CCC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 23.7% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 837,561 G→T 9.6% intergenic (‑134/+89) thiM ← / ← rcnR hydroxyethylthiazole kinase/Ni(II)/Co(II)‑binding transcriptional repressor RcnR
RA NZ_CP022686 878,938 T→G 8.6% pseudogene (752/762 nt) yohF ← SDR family oxidoreductase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 939,755 A→C 8.9% pseudogene (6/468 nt) CIW80_RS26375 → icd‑like protein
RA NZ_CP022686 948,090 C→A 14.2% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 11.9% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,073 A→C 7.0% E376D (GAA→GAC nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 13.9% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,890 T→G 12.9% V226G (GTC→GGC)  rbn → ribonuclease BN
RA NZ_CP022686 1,040,909 A→C 11.4% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,058,297 G→T 9.9% S97Y (TCC→TAC)  nuoC ← NADH‑quinone oxidoreductase subunit C/D
JC NZ_CP022686 1,061,329 Δ6 bp 6.1% coding (42‑47/945 nt) lrhA ← transcriptional regulator LrhA
RA NZ_CP022686 1,065,997 A→C 12.5% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,174,272 A→C 20.3% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,199,883 T→G 6.2% N147T (AAC→ACC)  yfeX ← porphyrinogen peroxidase
RA NZ_CP022686 1,203,715 T→G 8.8% G238G (GGT→GGG hemF → oxygen‑dependent coproporphyrinogen oxidase
RA NZ_CP022686 1,209,908 T→G 15.9% D396A (GAC→GCC)  eutH ← ethanolamine utilization protein EutH
RA NZ_CP022686 1,280,117 T→G 8.3% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,724 G→T 13.1% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,727 A→T 7.4% intergenic (‑62/+262) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 15.5% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,371,066 T→A 9.6% V27E (GTA→GAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,396,426 T→G 7.3% intergenic (+32/‑205) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,479,607 A→C 13.0% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,328 G→T 15.8% M255I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 13.6% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 19.4% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,110 G→C 23.1% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 12.2% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,569 A→C 11.0% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,868 C→A 24.0% E246* (GAA→TAA)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,888 G→T 12.8% intergenic (+34/‑17) ssnA → / → ygfM putative aminohydrolase SsnA/molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 15.2% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 12.7% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 17.6% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 10.9% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,032 G→T 12.9% intergenic (‑642/‑13) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,047 G→T 13.0% M1M (ATG→ATT) † metK → methionine adenosyltransferase
RA NZ_CP022686 1,735,915 T→G 10.0% pseudogene (354/569 nt) papA ← P fimbria major subunit PapA
RA NZ_CP022686 1,828,110 A→C 8.1% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,833,745 T→G 13.8% N468H (AAC→CAC)  gspD ← type II secretion system secretin GspD
RA NZ_CP022686 1,954,923 T→G 15.5% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,954,962 G→T 9.3% Q79K (CAA→AAA)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 1,965,159 A→C 9.4% L91R (CTG→CGG)  nfeF ← NADPH‑dependent ferric chelate reductase
RA NZ_CP022686 2,016,153 T→G 8.8% noncoding (121/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,160 T→G 6.0% noncoding (114/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,170 A→C 7.1% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,047,987 A→C 5.8% R623R (CGT→CGG deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,089 T→G 14.6% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,049,094 G→T 9.4% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,056,168 A→C 6.5% G614G (GGT→GGG infB ← translation initiation factor IF‑2
RA NZ_CP022686 2,072,584 A→C 8.6% A340A (GCT→GCG cgtA ← Obg family GTPase CgtA
RA NZ_CP022686 2,076,554 T→G 10.5% intergenic (+24/‑209) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,076,556 C→G 7.4% intergenic (+26/‑207) ispB → / → sfsB octaprenyl diphosphate synthase/DNA‑binding transcriptional regulator SfsB
RA NZ_CP022686 2,110,457 T→G 13.2% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 9.7% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,148,115 T→G 14.0% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 9.5% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,152 G→T 8.0% Q96K (CAG→AAG)  smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 9.9% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 5.5% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,188,239 G→T 8.3% intergenic (‑108/+57) rplN ← / ← rpsQ 50S ribosomal protein L14/30S ribosomal protein S17
RA NZ_CP022686 2,202,109 G→T 6.5% D51Y (GAT→TAT)  gspI → type II secretion system minor pseudopilin GspI
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 6.4% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,305,971 G→T 8.3% S222* (TCA→TAA)  CIW80_RS11625 ← fimbrial biogenesis usher protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,393,013 T→G 6.6% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,410,556 A→C 7.2% pseudogene (2792/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,523 A→C 6.8% V19G (GTA→GGA)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 14.4% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 11.1% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,546,394 A→C 12.8% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,572,705 A→C 16.5% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 11.6% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,617,885 T→G 8.5% K190N (AAA→AAC ilvB ← acetolactate synthase large subunit
RA NZ_CP022686 2,758,153 A→C 8.1% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,808,145 T→G 5.9% V447G (GTG→GGG)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA NZ_CP022686 2,834,886 A→C 7.5% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 23.6% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 11.6% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,856 T→G 8.3% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,385 C→A 8.3% A54E (GCA→GAA)  CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,929,407 T→G 5.1% K787N (AAA→AAC ptsP ← phosphoenolpyruvate‑‑protein phosphotransferase
RA NZ_CP022686 3,020,229 T→G 13.5% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,136,940 T→G 11.9% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,136,948 G→T 7.4% L158L (CTC→CTA phnN ← ribose 1,5‑bisphosphokinase
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,265,084 T→G 15.6% N77T (AAC→ACC)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,281,004 T→G 11.5% F75V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,052 T→G 8.0% F91V (TTT→GTT)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,281,062 T→G 12.1% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,360 T→G 6.7% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,345 G→T 9.4% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 7.8% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,360 C→A 6.8% intergenic (‑176/‑24) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,390,614 G→T 9.4% A143A (GCC→GCA sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,629 C→A 9.9% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,845 A→C 8.6% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,223 G→T 15.4% intergenic (‑32/+3) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 17.5% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 15.6% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,482,803 T→G 6.8% D474A (GAC→GCC)  ettA ← energy‑dependent translational throttle protein EttA
RA NZ_CP022686 3,496,461 T→G 19.6% F276V (TTT→GTT)  thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 15.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,591 G→T 8.9% D260Y (GAT→TAT)  acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,102 A→T 6.1% intergenic (‑111/+1) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,661,518 C→A 11.7% C9F (TGC→TTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,703,648 A→C 6.4% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,367 T→G 20.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 16.1% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,736,542 C→A 10.0% G70V (GGA→GTA)  mltD ← murein transglycosylase D
RA NZ_CP022686 3,758,887 C→A 11.6% K205N (AAG→AAT CIW80_RS18810 ← hypothetical protein
RA NZ_CP022686 3,770,508 T→G 7.9% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,509 A→T 11.2% intergenic (‑245/+218) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 7.7% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 10.3% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 8.8% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
RA NZ_CP022686 3,806,265 T→A 10.0% Y1586N (TAC→AAC)  cdiA → contact‑dependent inhibition effector tRNA nuclease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,850,155 T→G 10.7% intergenic (+53/‑39) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,852,459 T→G 9.1% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 9.1% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,895,500 G→T 5.7% intergenic (‑106/+78) CIW80_RS19510 ← / ← CIW80_RS19515 protein YkgH/autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 3,910,616 C→A 12.4% G145G (GGG→GGT yahC ← DUF1097 domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 14.1% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 10.1% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,376 T→G 10.3% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 11.1% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,671 T→A 5.5% M326L (ATG→TTG)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,707 A→C 9.5% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,940,724 A→C 9.3% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 11.0% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 16.7% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 3,984,841 T→G 8.2% R67R (CGT→CGG queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase QueA
RA NZ_CP022686 4,014,238 C→G 13.9% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,242 A→T 9.6% intergenic (+88/‑256) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,247 G→T 13.6% intergenic (+93/‑251) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,033,250 T→G 5.4% V303G (GTT→GGT)  amtB → ammonium transporter AmtB
RA NZ_CP022686 4,133,850 T→G 8.2% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,164,959 T→G 5.9% D113A (GAC→GCC)  dsbG ← thiol:disulfide interchange protein DsbG
RA NZ_CP022686 4,368,341 T→G 15.2% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,412,284 A→C 21.8% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,433,082 A→C 5.4% L279V (TTA→GTA)  cydC ← cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC
RA NZ_CP022686 4,435,749 A→C 7.5% intergenic (‑66/+57) cydD ← / ← trxB cysteine/glutathione ABC transporter permease/ATP‑binding protein CydD/thioredoxin‑disulfide reductase
RA NZ_CP022686 4,632,182 T→G 9.5% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,632,186 G→T 7.3% G278C (GGC→TGC)  mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,444 A→C 5.0% intergenic (+17/‑355) CIW80_RS23235 → / → focA S/F1C fimbrial major subunit operon transcriptional regulator/F1C fimbrial major subunit FocA
RA NZ_CP022686 4,655,834 A→C 6.0% R390R (CGT→CGG iroC ← salmochelin/enterobactin export ABC transporter IroC
RA NZ_CP022686 4,764,877 T→G 13.6% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,179 A→C 15.0% intergenic (‑78/+92) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,809,441 T→G 8.9% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,809,481 T→G 8.5% S64A (TCA→GCA)  CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,961 T→G 10.2% F61C (TTT→TGT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 13.2% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,905,178 T→G 13.6% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase
RA NZ_CP022686 4,986,440 A→C 6.6% L68V (TTA→GTA)  pspF ← phage shock protein operon transcriptional activator
RA NZ_CP022686 5,001,164 A→C 11.1% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,029,522 A→C 7.1% intergenic (‑113/+28) uspF ← / ← ompN universal stress protein UspF/porin OmpN
RA NZ_CP022686 5,041,113 T→C 7.3% N248N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 9.1% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 6.3% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 11.3% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 12.2% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,137 G→T 9.9% V1256V (GTG→GTT CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.1% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 93.4% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 682–2618 3042–2957 340–2361 6462 [6147] [6181] 6305 JNG15_RS00010–mobA JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1260–1355 3987–1581 227–2728 6054 [6039] [5642] 6363 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =0 (0.000)211 (1.050) 78/248 NT 100% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624680 (0.000)178 (0.890) 66/248 NT 100% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 = 2516264218 (1.090)9 (0.050) 5/242 NT 6.0% coding (1674/5337 nt) upaG trimeric autotransporter adhesin UpaG
?NZ_CP022686 2516754 = 71 (0.360)coding (2164/5337 nt) upaG trimeric autotransporter adhesin UpaG
* ? NZ_CP022686 3899666 =163 (0.810)70 (0.400) 38/216 NT 33.1% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 141 (0.810)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681153 (0.760)80 (0.460) 48/216 NT 36.8% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 141 (0.810)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =4299 (0.440)6068 (0.620) 229/248 NT 66.6% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1774 (0.180)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4916319 (0.640)4958 (0.630) 176/198 NT 59.3% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1772 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–