breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 28,280 C→A 10.3% P24T (CCC→ACC)  ydcV → ABC transporter permease
RA NZ_CP022686 177,506 T→G 12.4% Q140H (CAA→CAC uidB ← glucuronide transporter
RA NZ_CP022686 182,646 A→C 10.9% G68G (GGT→GGG malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 349,106 A→C 20.5% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 18.6% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 22.6% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 21.2% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 694,402 G→T 14.1% intergenic (+162/‑34) CIW80_RS03555 → / → CIW80_RS03560 transposase/PfkB family carbohydrate kinase
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,452 G→T 22.2% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 18.5% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 20.2% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,000,443 T→G 15.6% D941A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,082 T→A 9.9% Y379* (TAT→TAA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,005,085 C→A 11.3% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,100,944 T→G 16.5% D187A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,137,218 A→C 6.9% V247G (GTT→GGT)  emrK ← multidrug efflux MFS transporter periplasmic adaptor subunit EmrK
RA NZ_CP022686 1,174,272 A→C 20.9% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,269,551 T→G 13.6% K1124N (AAA→AAC CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,280,117 T→G 7.5% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit YfgM
RA NZ_CP022686 1,360,724 G→T 8.5% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,729 G→T 7.6% intergenic (‑64/+260) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,750 G→T 7.5% intergenic (‑85/+239) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,371,081 C→A 20.2% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD
RA NZ_CP022686 1,443,501 A→C 8.3% V20G (GTC→GGC)  hypF ← carbamoyltransferase HypF
RA NZ_CP022686 1,479,607 A→C 12.6% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,289 T→G 10.4% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,353 G→C 8.6% A264P (GCT→CCT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 13.1% A264A (GCT→GCA) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,361 G→T 15.8% V266V (GTG→GTT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,566,203 A→C 10.2% N735T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,633,846 T→G 29.9% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,640,925 A→C 19.7% R7S (AGA→AGC ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA NZ_CP022686 1,674,224 T→G 14.8% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,231 A→T 13.0% V186E (GTA→GAA)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 13.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.4% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,758,771 T→G 6.3% intergenic (+18/‑254) sat → / → CIW80_RS08700 serine protease autotransporter toxin Sat/transposase
RA NZ_CP022686 1,805,047 G→T 10.9% pseudogene (328/934 nt) CIW80_RS09020 → nucleotidyl transferase AbiEii/AbiGii toxin family protein
RA NZ_CP022686 1,828,110 A→C 9.1% R85R (CGT→CGG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 2,049,094 G→T 8.3% T254T (ACC→ACA deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,110,457 T→G 10.3% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 8.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,109 T→G 14.5% V124G (GTG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,115 T→G 14.3% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 12.1% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,139 C→G 14.9% A134G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,141 T→G 14.9% F135V (TTC→GTC)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,172,165 G→T 6.9% F91L (TTC→TTA smg ← DUF494 family protein Smg
RA NZ_CP022686 2,185,075 A→C 9.6% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,267,025 A→C 5.3% F50V (TTT→GTT)  yhgE ← DUF4153 domain‑containing protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,380,933 G→C 9.3% K185N (AAG→AAC pitA → inorganic phosphate transporter PitA
RA NZ_CP022686 2,394,287 T→G 20.4% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,397,370 T→G 9.2% F9C (TTT→TGT)  chuT → heme ABC transporter substrate‑binding protein ChuT
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,576 C→A 14.1% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,470,422 T→G 10.9% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,516,426 T→A 12.5% G612G (GGT→GGA upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,574,429 T→G 13.2% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,701,400 G→T 7.5% R328S (CGC→AGC)  mdtD ← multidrug transporter subunit MdtD
RA NZ_CP022686 2,717,385 T→G 10.9% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,717,400 T→G 7.0% L101V (TTA→GTA)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,585 T→A 10.2% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 11.4% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,743,563 T→G 9.4% noncoding (65/87 nt) CIW80_RS13750 → tRNA‑Leu
RA NZ_CP022686 2,743,613 C→A 19.9% intergenic (+28/‑15) CIW80_RS13750 → / → CIW80_RS13755 tRNA‑Leu/tRNA‑Pro
RA NZ_CP022686 2,769,166 C→A 12.7% C57F (TGC→TTC)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,769,196 A→C 20.7% F47C (TTT→TGT)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,780,110 T→G 7.2% F677C (TTT→TGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
RA NZ_CP022686 2,868,740 T→G 24.5% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 11.6% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,904,852 T→G 9.3% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 3,070,955 C→A 15.4% S43* (TCA→TAA)  CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,071,004 T→G 11.3% G59G (GGT→GGG CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,387,644 A→C 11.6% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,625 C→A 19.8% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,390,629 C→A 17.9% W138C (TGG→TGT sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,461,215 A→T 14.5% intergenic (‑24/+11) CIW80_RS17335 ← / ← CIW80_RS17340 tRNA‑Leu/tRNA‑Leu
RA NZ_CP022686 3,461,229 T→G 17.7% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,247 A→C 12.2% noncoding (66/87 nt) CIW80_RS17340 ← tRNA‑Leu
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,604,564 G→T 13.7% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,634,614 A→C 13.6% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 11.7% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,703,648 A→C 8.0% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,734,380 T→G 13.6% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,508 T→G 11.3% intergenic (‑244/+219) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,770,520 G→T 10.0% intergenic (‑256/+207) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,850,155 T→G 9.9% intergenic (+53/‑39) gpt → / → frsA xanthine phosphoribosyltransferase/esterase FrsA
RA NZ_CP022686 3,868,846 T→G 7.9% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,868,857 T→G 11.3% N379T (AAC→ACC)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,925,289 A→C 17.2% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,935,324 T→G 10.8% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 14.7% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,949,969 T→G 7.6% D141A (GAC→GCC)  hemB ← porphobilinogen synthase
RA NZ_CP022686 3,981,933 T→G 13.2% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,004,135 T→G 9.5% R29R (CGT→CGG yajQ → nucleotide binding protein YajQ
RA NZ_CP022686 4,014,225 C→G 9.8% intergenic (+71/‑273) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,238 C→G 15.6% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,014,291 G→T 24.0% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 18.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,332 A→C 9.9% W169G (TGG→GGG)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,290,599 T→G 10.3% T121P (ACC→CCC)  ybgS ← YbgS‑like family protein
RA NZ_CP022686 4,439,443 A→C 5.9% K500N (AAA→AAC ftsK → DNA translocase FtsK
RA NZ_CP022686 4,615,712 G→T 40.3% G122C (GGT→TGT)  CIW80_RS23065 → hypothetical protein
RA NZ_CP022686 4,764,877 T→G 17.3% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,883 A→T 11.5% intergenic (‑63/‑198) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,914 C→A 12.8% intergenic (‑94/‑167) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,918,873 T→G 6.1% Q378H (CAA→CAC adhE ← bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
RA NZ_CP022686 5,041,113 T→C 5.7% N248N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMUT1‑hok‑empty 4,123:1 +T 94.5% intergenic (‑341/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,127 C→A 100% intergenic (‑345/–) putative rom (RNA I inhibition modulator protein) ← /  –/–
RA pMUT1‑hok‑empty 4,132 4 bp→TA 100% intergenic (‑350/–) putative rom (RNA I inhibition modulator protein) ← /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 188–2618 3084–2957 340–2897 9931 [9629] [8996] 9841 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMUT1‑hok‑empty 1263–1318 3987–1582 265–2725 4145 [3898] [3967] 4660 putative HTH domain protein–putative rom (RNA I inhibition modulator protein) putative HTH domain protein,uncharacterized protein,hypothetical protein,putative rom (RNA I inhibition modulator protein)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =4 (0.030)123 (0.780) 55/250 NT 96.9% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624684 (0.030)120 (0.760) 57/250 NT 96.8% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
* ? NZ_CP022686 3899666 =202 (1.280)12 (0.090) 10/218 NT 6.9% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 150 (1.090)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681186 (1.180)10 (0.070) 7/218 NT 6.0% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 150 (1.090)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? pMUT1‑hok‑empty 456 =3108 (0.460)4241 (0.630) 215/250 NT 66.0% intergenic (–/+1261) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty 4136 = 1253 (0.190)intergenic (‑354/–) putative rom (RNA I inhibition modulator protein)/– –/–
* ? pMUT1‑hok‑empty = 4914583 (0.680)3461 (0.650) 175/200 NT 58.5% intergenic (–/+1226) –/putative HTH domain protein –/–
?pMUT1‑hok‑empty = 4098 1248 (0.230)intergenic (‑316/–) putative rom (RNA I inhibition modulator protein)/– –/–