breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC697,1070TG60.0% 2.2 / 21.9 29intergenic (+34/‑717)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC697,1000TG59.3% 0.3 / 10.6 27intergenic (+27/‑724)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC5,164,2070AC58.8% 5.4 / 19.3 18intergenic (+27/+174)PP_4544/PP_4545hypothetical protein/beta‑ketodecanoyl‑ACP synthase
*NC_002947_CJ‑RC697,0940AG57.7% ‑3.0 / 18.2 27intergenic (+21/‑730)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC1,327,1210CT55.9% 17.5 / 190.9 145intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947_CJ‑RC4,348,8120AG54.6% ‑1.8 / 138.6 108intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_002947_CJ‑RC1,296,5670GT52.9% 2.8 / 17.0 17intergenic (‑320/‑246)nhaA‑I/PP_1133Na(+)/H(+) antiporter NhaA/transposase
*NC_002947_CJ‑RC4,158,4660TG52.0% 2.7 / 18.7 28L159R (CTG→CGG) PP_3661membrane protein
*NC_002947_CJ‑RC5,161,5750GC46.4% 16.0 / 13.9 28R395G (CGG→GGG) PP_4543hypothetical protein
*NC_002947_CJ‑RC699,7220AG42.9% 41.1 / 123.1 98intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_002947_CJ‑RC1,703,2480AG38.1% 12.4 / 17.1 21intergenic (+97/‑70)lysS/PP_1497lysine‑‑tRNA ligase/TetR family transcriptional regulator
*NC_002947_CJ‑RC1,703,2500GC38.1% 21.5 / 18.5 21intergenic (+99/‑68)lysS/PP_1497lysine‑‑tRNA ligase/TetR family transcriptional regulator
*NC_002947_CJ‑RC1,703,2520CT38.1% 14.6 / 17.3 21intergenic (+101/‑66)lysS/PP_1497lysine‑‑tRNA ligase/TetR family transcriptional regulator
*NC_002947_CJ‑RC4,935,5770TG36.0% 54.9 / 11.7 52Q192P (CAG→CCG) flhFflagellar biosynthesis protein FlhF
*NC_002947_CJ‑RC4,049,9070AG31.6% 16.3 / 12.2 22intergenic (+40/‑61)quiA/oprB‑IIIquinate dehydrogenase/carbohydrate‑selective porin
*NC_002947_CJ‑RC1,130,1990GT27.6% 125.2 / 12.3 58intergenic (‑254/+50)gcvH‑I/PP_0990glycine cleavage system protein H/DoxX family protein
*NC_002947_CJ‑RC3,985,6450AC27.5% 80.1 / 10.4 53intergenic (+74/‑79)PP_3513/oplBLysR family transcriptional regulator/5‑oxoprolinase B
*NC_002947_CJ‑RC4,044,5690AC25.7% 136.2 / 20.0 74V380G (GTG→GGG) PP_3566MFS transporter
*NC_002947_CJ‑RC4,433,5700CA25.4% 152.2 / 22.3 67intergenic (‑19/‑99)PP_5648/PP_3928hypothetical protein/hypothetical protein
*NC_002947_CJ‑RC1,316,1980CA24.2% 172.4 / 12.2 69intergenic (+73/‑28)PP_1148/PP_mr15hypothetical protein/P20 ncRNA

Marginal new junction evidence (lowest skew 10 of 34 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC 4809877 =13 (0.170)16 (0.280) 12/222 5.4 34.5% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC = 5991750 51 (0.890)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 48098822 (0.030)13 (0.200) 11/252 6.6 27.5% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC = 5991764 67 (1.030)intergenic (+243/‑303) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 178006612 (0.160)15 (0.210) 13/278 6.6 52.2% intergenic (+25/+81) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947_CJ‑RC 1780129 = 16 (0.220)intergenic (+88/+18) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947_CJ‑RC = 55584233 (0.040)11 (0.150)
+GCC
11/286 7.5 78.9% intergenic (‑84/+87) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
?NC_002947_CJ‑RC 5558424 = 3 (0.040)intergenic (‑85/+86) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
* ? NC_002947_CJ‑RC = 56842303 (0.040)10 (0.140)
+CGGG
9/284 8.3 77.4% intergenic (‑133/+71) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
?NC_002947_CJ‑RC 5684231 = 3 (0.040)intergenic (‑134/+70) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
* ? NC_002947_CJ‑RC = 295708039 (0.520)9 (0.120) 9/282 8.3 18.2% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947_CJ‑RC 2957165 = 43 (0.590)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
* ? NC_002947_CJ‑RC = 141954916 (0.210)12 (0.160) 9/286 8.4 40.2% intergenic (‑38/+98) PP_1242/PP_1244 hypothetical protein/hypothetical protein
?NC_002947_CJ‑RC = 1419636 20 (0.270)intergenic (‑125/+11) PP_1242/PP_1244 hypothetical protein/hypothetical protein
* ? NC_002947_CJ‑RC 4809877 =13 (0.170)6 (0.100) 5/240 9.2 15.1% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC 5991594 = 57 (0.920)intergenic (+73/‑473) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC 1967145 =33 (0.440)6 (0.100) 4/226 9.4 22.9% intergenic (+15/+104) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947_CJ‑RC 1967241 = 15 (0.260)intergenic (+111/+8) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947_CJ‑RC = 153173118 (0.240)8 (0.110) 6/278 9.7 26.2% coding (145/630 nt) PP_1344 hypothetical protein
?NC_002947_CJ‑RC 1531839 = 28 (0.390)coding (37/630 nt) PP_1344 hypothetical protein