breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,338,6080GT60.0% 10.5 / 23.1 22intergenic (+121/+21)PP_3812/PP_5632lipase/allo‑isoleucine metabolism protein
*NC_002947_CJ‑RC197,2460CT55.1% 23.6 / 227.3 168Q918* (CAG→TAG) PP_0168putative surface adhesion protein
*NC_002947_CJ‑RC697,1070TG55.0% 9.0 / 26.1 24intergenic (+34/‑717)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC1,327,1210CT52.8% 45.6 / 222.9 161intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947_CJ‑RC697,0940AG52.2% ‑3.2 / 23.0 24intergenic (+21/‑730)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC4,348,8120AG48.2% 41.2 / 203.5 137intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_002947_CJ‑RC1,842,8160TC47.8% 3.4 / 15.2 24S660G (AGC→GGC) gacSsensor protein GacS
*NC_002947_CJ‑RC4,158,4710AC47.8% 15.1 / 19.6 50S161R (AGC→CGC) PP_3661membrane protein
*NC_002947_CJ‑RC699,7220AG40.3% 92.6 / 148.0 129intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_002947_CJ‑RC4,740,9480TA38.9% 8.3 / 11.7 19intergenic (+78/+675)gltA/yeiWcitrate synthase/putative oxidoreductase
*NC_002947_CJ‑RC4,740,9510CG38.9% 5.0 / 19.1 18intergenic (+81/+672)gltA/yeiWcitrate synthase/putative oxidoreductase
*NC_002947_CJ‑RC4,740,9460CG36.8% 4.8 / 18.9 19intergenic (+76/+677)gltA/yeiWcitrate synthase/putative oxidoreductase
*NC_002947_CJ‑RC3,447,2860AC36.2% 33.7 / 11.4 50S18R (AGC→CGC) PP_5556bacteriophage lysis protein
*NC_002947_CJ‑RC1,130,1990GT35.4% 103.4 / 20.7 65intergenic (‑254/+50)gcvH‑I/PP_0990glycine cleavage system protein H/DoxX family protein
*NC_002947_CJ‑RC5,905,9800AC35.0% 11.6 / 12.3 21intergenic (‑129/‑139)PP_5175/PP_5176HlyD family secretion protein/hypothetical protein
*NC_002947_CJ‑RC5,512,7200TG34.0% 36.6 / 11.7 49L35R (CTG→CGG) urtBurea ABC transporter permease
*NC_002947_CJ‑RC4,740,9490TA31.6% 18.2 / 10.6 19intergenic (+79/+674)gltA/yeiWcitrate synthase/putative oxidoreductase
*NC_002947_CJ‑RC5,174,9350CA31.6% 64.0 / 11.2 38intergenic (‑273/+59)fadD‑I/fadD‑IIlong‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
*NC_002947_CJ‑RC5,773,9490TG29.7% 113.7 / 14.3 64E610A (GAG→GCG) betT‑Icholine transporter
*NC_002947_CJ‑RC2,282,7700TG29.6% 38.2 / 16.5 27intergenic (‑162/+67)PP_2010/PP_2011cytochrome b561/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 35 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 45861365 (0.060)18 (0.230) 16/270 6.3 58.4% intergenic (+112/+103) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947_CJ‑RC = 4586216 21 (0.270)intergenic (+192/+23) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947_CJ‑RC = 295708041 (0.480)19 (0.230) 17/282 6.3 31.8% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947_CJ‑RC 2957165 = 42 (0.510)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
* ? NC_002947_CJ‑RC = 56842301 (0.010)20 (0.240)
+CGGG
16/284 6.6 95.4% intergenic (‑133/+71) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
?NC_002947_CJ‑RC 5684231 = 1 (0.010)intergenic (‑134/+70) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
* ? NC_002947_CJ‑RC = 55584233 (0.040)16 (0.190)
+GCC
14/286 7.3 84.5% intergenic (‑84/+87) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
?NC_002947_CJ‑RC 5558424 = 3 (0.040)intergenic (‑85/+86) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
* ? NC_002947_CJ‑RC = 48098821 (0.010)12 (0.150) 11/268 8.0 28.6% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC 5991580 = 59 (0.750)intergenic (+59/‑487) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 599158658 (0.680)10 (0.120) 10/276 8.6 13.7% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991778 = 71 (0.880)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC 4809877 =5 (0.060)9 (0.140) 5/222 9.6 23.8% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC = 5991750 54 (0.830)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 17800668 (0.090)9 (0.110) 8/278 9.6 50.5% intergenic (+25/+81) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947_CJ‑RC 1780129 = 10 (0.120)intergenic (+88/+18) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947_CJ‑RC = 599157268 (0.800)6 (0.070) 6/276 10.7 8.2% intergenic (+51/‑495) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991794 = 71 (0.880)intergenic (+273/‑273) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 495927578 (0.910)7 (0.090) 5/262 10.9 8.2% intergenic (‑55/+108) fliH/fliG flagellar assembly protein FliH/flagellar motor switch protein
?NC_002947_CJ‑RC = 4959335 87 (1.140)intergenic (‑115/+48) fliH/fliG flagellar assembly protein FliH/flagellar motor switch protein