breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,158,4660TG54.2% 3.5 / 23.1 24L159R (CTG→CGG) PP_3661membrane protein
*NC_002947_CJ‑RC1,327,1210CT53.4% 31.6 / 161.5 118intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947_CJ‑RC697,0940AG52.2% ‑0.8 / 24.7 25intergenic (+21/‑730)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC4,348,8120AG50.0% 20.7 / 125.0 82intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_002947_CJ‑RC699,7220AG47.9% 15.9 / 129.0 94intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_002947_CJ‑RC4,158,4710AC45.8% 4.4 / 10.4 24S161R (AGC→CGC) PP_3661membrane protein
*NC_002947_CJ‑RC5,512,7110AC45.5% 3.3 / 12.5 37Q32P (CAG→CCG) urtBurea ABC transporter permease
*NC_002947_CJ‑RC2,282,7750GC33.3% 29.7 / 20.9 24intergenic (‑167/+62)PP_2010/PP_2011cytochrome b561/hypothetical protein
*NC_002947_CJ‑RC2,282,7790CA33.3% 29.7 / 17.6 22intergenic (‑171/+58)PP_2010/PP_2011cytochrome b561/hypothetical protein
*NC_002947_CJ‑RC1,544,3590CG31.2% 59.0 / 12.0 32A388G (GCG→GGG) mdfAmultidrug MFS transporter
*NC_002947_CJ‑RC4,242,2290TG31.2% 85.3 / 11.3 48D203A (GAC→GCC) PP_3719putrescine‑binding protein
*NC_002947_CJ‑RC2,282,7700TG30.4% 22.9 / 21.1 24intergenic (‑162/+67)PP_2010/PP_2011cytochrome b561/hypothetical protein
*NC_002947_CJ‑RC1,751,0590AG29.3% 60.3 / 10.9 43T140A (ACC→GCC) PP_1551replication protein O
*NC_002947_CJ‑RC2,282,7740GC29.2% 32.1 / 19.5 24intergenic (‑166/+63)PP_2010/PP_2011cytochrome b561/hypothetical protein
*NC_002947_CJ‑RC4,433,5800GC27.7% 87.0 / 18.9 47intergenic (‑29/‑89)PP_5648/PP_3928hypothetical protein/hypothetical protein
*NC_002947_CJ‑RC2,821,3950G.25.0% 32.8 / 10.1 17intergenic (‑64/‑327)PP_2474/PP_2475glutathione S‑transferase family protein/TetR family transcriptional regulator
*NC_002947_CJ‑RC248,8610CA24.6% 140.4 / 13.3 57Q148K (CAA→AAA) PP_0198putative transporter
*NC_002947_CJ‑RC1,898,6570TG23.8% 99.9 / 10.7 42intergenic (+63/‑108)PP_t16/PP_t17tRNA‑Asp/tRNA‑Val
*NC_002947_CJ‑RC1,544,3650TG21.9% 57.2 / 10.6 32V390G (GTG→GGG) mdfAmultidrug MFS transporter
*NC_002947_CJ‑RC3,207,6790GC21.4% 94.1 / 11.2 42S443T (AGC→ACC) PP_2812transporter

Marginal new junction evidence (lowest skew 10 of 22 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 55584232 (0.030)19 (0.320)
+GCC
15/286 3.9 90.6% intergenic (‑84/+87) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
?NC_002947_CJ‑RC 5558424 = 2 (0.030)intergenic (‑85/+86) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
* ? NC_002947_CJ‑RC = 65278248 (0.780)12 (0.230) 9/252 5.0 36.7% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947_CJ‑RC = 4809862 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947_CJ‑RC = 599157256 (0.910)8 (0.140) 8/276 5.9 14.3% intergenic (+51/‑495) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991794 = 43 (0.740)intergenic (+273/‑273) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 599158655 (0.900)7 (0.120) 7/276 6.3 14.1% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991778 = 33 (0.570)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC 5126790 =44 (0.720)5 (0.100) 5/240 6.5 14.9% intergenic (+61/‑121) PP_4511/PP_4512 AraC family transcriptional regulator/DMT superfamily permease
?NC_002947_CJ‑RC 5126869 = 21 (0.420)intergenic (+140/‑42) PP_4511/PP_4512 AraC family transcriptional regulator/DMT superfamily permease
* ? NC_002947_CJ‑RC = 455208011 (0.180)4 (0.080) 4/226 6.7 26.2% intergenic (+67/+114) pydA/rutR NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA/transcriptional regulator, TetR family
?NC_002947_CJ‑RC = 4552131 14 (0.300)intergenic (+118/+63) pydA/rutR NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA/transcriptional regulator, TetR family
* ? NC_002947_CJ‑RC 5916972 =29 (0.470)4 (0.080) 4/236 6.9 18.4% intergenic (+49/+104) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
?NC_002947_CJ‑RC 5917068 = 12 (0.240)intergenic (+145/+8) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
* ? NC_002947_CJ‑RC = 56842300 (0.000)7 (0.120)
+CGGG
6/284 6.9 100% intergenic (‑133/+71) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
?NC_002947_CJ‑RC 5684231 = 0 (0.000)intergenic (‑134/+70) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
* ? NC_002947_CJ‑RC = 295708028 (0.460)6 (0.100) 6/282 6.9 17.6% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947_CJ‑RC 2957165 = 29 (0.490)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
* ? NC_002947_CJ‑RC 1419549 =26 (0.420)4 (0.080) 3/234 7.5 18.3% intergenic (‑38/+98) PP_1242/PP_1244 hypothetical protein/hypothetical protein
?NC_002947_CJ‑RC 1419637 = 15 (0.310)intergenic (‑126/+10) PP_1242/PP_1244 hypothetical protein/hypothetical protein