mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 65_S65_L001_R1_001.good.fq | 325,500 | 47,823,944 | 100.0% | 146.9 bases | 149 bases | 99.1% |
| errors | 65_S65_L001_R2_001.good.fq | 325,500 | 47,822,771 | 100.0% | 146.9 bases | 149 bases | 98.1% |
| errors | 65_S65_L002_R1_001.good.fq | 311,975 | 45,826,953 | 100.0% | 146.9 bases | 149 bases | 99.1% |
| errors | 65_S65_L002_R2_001.good.fq | 311,975 | 45,829,529 | 100.0% | 146.9 bases | 149 bases | 97.7% |
| errors | 65_S65_L003_R1_001.good.fq | 327,059 | 48,045,689 | 100.0% | 146.9 bases | 149 bases | 99.2% |
| errors | 65_S65_L003_R2_001.good.fq | 327,059 | 48,045,328 | 100.0% | 146.9 bases | 149 bases | 98.3% |
| errors | 65_S65_L004_R1_001.good.fq | 336,253 | 49,388,539 | 100.0% | 146.9 bases | 149 bases | 99.2% |
| errors | 65_S65_L004_R2_001.good.fq | 336,253 | 49,384,806 | 100.0% | 146.9 bases | 149 bases | 98.6% |
| total | 2,601,574 | 382,167,559 | 100.0% | 146.9 bases | 149 bases | 98.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947_CJ-RC | 6,184,688 | 61.6 | 1.8 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,184,688 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 7380 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 505 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.025 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947_CJ-RC | 0.82904 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 14:43:11 25 Oct 2024 | 14:43:58 25 Oct 2024 | 47 seconds |
| Read alignment to reference genome | 14:43:58 25 Oct 2024 | 14:49:20 25 Oct 2024 | 5 minutes 22 seconds |
| Preprocessing alignments for candidate junction identification | 14:49:20 25 Oct 2024 | 14:49:55 25 Oct 2024 | 35 seconds |
| Preliminary analysis of coverage distribution | 14:49:55 25 Oct 2024 | 14:51:21 25 Oct 2024 | 1 minute 26 seconds |
| Identifying junction candidates | 14:51:21 25 Oct 2024 | 14:51:24 25 Oct 2024 | 3 seconds |
| Re-alignment to junction candidates | 14:51:24 25 Oct 2024 | 14:52:52 25 Oct 2024 | 1 minute 28 seconds |
| Resolving best read alignments | 14:52:52 25 Oct 2024 | 14:53:39 25 Oct 2024 | 47 seconds |
| Creating BAM files | 14:53:39 25 Oct 2024 | 14:54:58 25 Oct 2024 | 1 minute 19 seconds |
| Tabulating error counts | 14:54:58 25 Oct 2024 | 14:55:31 25 Oct 2024 | 33 seconds |
| Re-calibrating base error rates | 14:55:31 25 Oct 2024 | 14:55:34 25 Oct 2024 | 3 seconds |
| Examining read alignment evidence | 14:55:34 25 Oct 2024 | 15:11:16 25 Oct 2024 | 15 minutes 42 seconds |
| Polymorphism statistics | 15:11:16 25 Oct 2024 | 15:11:17 25 Oct 2024 | 1 second |
| Output | 15:11:17 25 Oct 2024 | 15:11:46 25 Oct 2024 | 29 seconds |
| Total | 28 minutes 35 seconds | ||
