breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC697,0940AG69.0% 14.7 / 14.9 32intergenic (+21/‑730)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC170,8330AG53.3% ‑2.8 / 15.2 16intergenic (+14/‑556)PP_0159/PP_16SAfamily III CoA‑transferase/16S ribosomal RNA
*NC_002947_CJ‑RC3,951,0920GT53.3% 11.8 / 19.2 15intergenic (‑56/+139)PP_3486/PP_3487cytochrome c/hypothetical protein
*NC_002947_CJ‑RC1,327,1210CT51.2% 55.3 / 234.8 160intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947_CJ‑RC4,348,8120AG50.8% 34.0 / 188.2 133intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_002947_CJ‑RC697,1070TG48.1% 8.5 / 12.8 33intergenic (+34/‑717)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC2,653,6180CA47.6% 5.1 / 11.4 21intergenic (+57/‑68)PP_2325/PP_23262‑dehydropantoate 2‑reductase/universal stress protein
*NC_002947_CJ‑RC1,712,0750GT47.1% ‑0.1 / 10.0 17intergenic (+66/‑192)ppc/adkphosphoenolpyruvate carboxylase/adenylate kinase
*NC_002947_CJ‑RC699,7220AG45.8% 35.6 / 160.0 118intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_002947_CJ‑RC4,158,4710AC45.7% 2.8 / 14.7 35S161R (AGC→CGC) PP_3661membrane protein
*NC_002947_CJ‑RC697,1140TG44.4% 27.1 / 14.3 37intergenic (+41/‑710)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC195,6340CT36.4% 129.6 / 121.8 133T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_002947_CJ‑RC697,0970CG33.3% 23.4 / 11.1 33intergenic (+24/‑727)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC2,863,8290TG31.9% 55.5 / 11.9 47S297A (TCG→GCG) gllRHTH‑type transcriptional regulator GalR
*NC_002947_CJ‑RC1,130,1850CA30.9% 78.3 / 12.0 55intergenic (‑240/+64)gcvH‑I/PP_0990glycine cleavage system protein H/DoxX family protein
*NC_002947_CJ‑RC697,1280TG30.8% 40.0 / 10.2 39intergenic (+55/‑696)mmsA‑I/PP_16SDmethylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
*NC_002947_CJ‑RC5,442,1260CG30.0% 40.4 / 11.7 30R15R (CGG→CGCrluE23S rRNA pseudouridine(2457) synthase
*NC_002947_CJ‑RC3,941,2400GC28.2% 153.4 / 11.8 71G106G (GGC→GGGPP_3476type II secretion system protein G
*NC_002947_CJ‑RC5,754,5480CA27.9% 83.5 / 11.6 48intergenic (‑171/‑300)thiI/glnAtRNA sulfurtransferase/glutamine synthetase
*NC_002947_CJ‑RC5,181,2050CG27.5% 116.2 / 12.6 52intergenic (‑179/+13)PP_4556/PP_4557hypothetical protein/hypothetical protein

Marginal new junction evidence (lowest skew 10 of 32 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC 1737987 =88 (1.040)25 (0.330) 20/256 5.0 23.1% coding (32/348 nt) yffB glutathione‑dependent thiol reductase
?NC_002947_CJ‑RC = 1777401 89 (1.190)intergenic (+135/+157) PP_5476/PP_1585 hypothetical protein/antidote protein
* ? NC_002947_CJ‑RC = 65278282 (0.970)20 (0.270) 17/250 5.7 35.5% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947_CJ‑RC = 4809862 2 (0.030)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947_CJ‑RC = 48098824 (0.050)18 (0.230) 18/266 5.8 41.1% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC 5991580 = 48 (0.620)intergenic (+59/‑487) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 295708040 (0.470)20 (0.240) 19/280 5.9 32.1% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947_CJ‑RC 2957165 = 46 (0.560)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
* ? NC_002947_CJ‑RC 4809877 =11 (0.130)18 (0.280) 11/220 6.8 41.2% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC = 5991750 43 (0.670)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 55584233 (0.040)16 (0.190)
+GCC
16/284 7.0 84.5% intergenic (‑84/+87) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
?NC_002947_CJ‑RC 5558424 = 3 (0.040)intergenic (‑85/+86) PP_4887/PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
* ? NC_002947_CJ‑RC = 56842300 (0.000)14 (0.170)
+CGGG
13/282 8.0 100% intergenic (‑133/+71) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
?NC_002947_CJ‑RC 5684231 = 0 (0.000)intergenic (‑134/+70) PP_4986/PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
* ? NC_002947_CJ‑RC = 141954916 (0.190)19 (0.230) 13/284 8.1 50.2% intergenic (‑38/+98) PP_1242/PP_1244 hypothetical protein/hypothetical protein
?NC_002947_CJ‑RC = 1419636 22 (0.260)intergenic (‑125/+11) PP_1242/PP_1244 hypothetical protein/hypothetical protein
* ? NC_002947_CJ‑RC = 599158656 (0.660)12 (0.150) 12/274 8.2 17.3% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991778 = 62 (0.770)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 599157257 (0.670)11 (0.140) 11/274 8.6 15.0% intergenic (+51/‑495) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991794 = 71 (0.890)intergenic (+273/‑273) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA