breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 195,559 C→A 23.0% T355T (ACC→ACA PP_0168 → putative surface adhesion protein
RA 195,628 C→T 10.5% D378D (GAC→GAT PP_0168 → putative surface adhesion protein
RA 195,629 A→G 10.7% T379A (ACC→GCC)  PP_0168 → putative surface adhesion protein
RA 195,634 C→T 32.5% T380T (ACC→ACT PP_0168 → putative surface adhesion protein
RA 196,306 T→C 28.4% N604N (AAT→AAC PP_0168 → putative surface adhesion protein
RA 196,327 A→C 27.8% T611T (ACA→ACC PP_0168 → putative surface adhesion protein
RA 196,351 C→G 31.0% T619T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 196,390 T→C 30.0% D632D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 197,032 C→T 6.1% T846T (ACC→ACT PP_0168 → putative surface adhesion protein
RA 197,089 T→C 13.8% N865N (AAT→AAC PP_0168 → putative surface adhesion protein
RA 197,179 A→G 8.3% V895V (GTA→GTG PP_0168 → putative surface adhesion protein
RA 197,185 C→T 6.9% T897T (ACC→ACT PP_0168 → putative surface adhesion protein
RA 197,551 C→G 13.0% T1019T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 197,572 G→A 22.3% K1026K (AAG→AAA PP_0168 → putative surface adhesion protein
RA 197,590 T→C 22.8% D1032D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 197,632 T→C 9.3% T1046T (ACT→ACC PP_0168 → putative surface adhesion protein
RA 197,695 G→C 8.3% T1067T (ACG→ACC PP_0168 → putative surface adhesion protein
RA 224,748 C→T 11.2% G233G (GGC→GGT PP_0172 → ABC transporter permease
RA 278,742:1 +A 100% intergenic (‑237/+77) PP_0224 ← / ← sctC DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
RA 284,586 T→A 38.0% intergenic (+33/+30) betT‑II → / ← atsK choline/carnitine/betaine transporter/alpha‑ketoglutarate‑dependent sulfonate dioxygenase
RA 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein
RA 336,124:1 +T 100% coding (209/237 nt) PP_0278 → hypothetical protein
RA 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
RA 408,711 G→A 8.3% Y651Y (TAC→TAT aceE ← pyruvate dehydrogenase E1 component
RA 499,203 A→G 100% intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
RA 542,239 T→C 10.8% L160P (CTG→CCG)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA 549,164 C→T 100% L672F (CTC→TTC)  fusA → elongation factor G 1
RA 699,722 A→G 56.3% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA
RA 756,535 C→T 16.6% C377C (TGC→TGT PP_0646 → hypothetical protein
RA 766,980 C→A 6.5% L158M (CTG→ATG)  metB → cystathionine gamma‑synthase
RA 942,226 C→A 5.4% Q1132H (CAG→CAT PP_0806 ← surface adhesion protein
RA 1,070,246:1 +GA 100% intergenic (+46/+96) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
RA 1,126,645:1 +C 100% intergenic (‑99/+50) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase
JC 1,311,719 Δ84 bp 22.4% intergenic (‑187/+8) PP_1144 ← / ← rapA membrane protein/RNA polymerase‑associated protein RapA
RA 1,327,121 C→T 52.4% intergenic (+97/‑196) PP_16SE → / → PP_23SE 16S ribosomal RNA/23S ribosomal RNA
RA 1,387,759 G→A 7.5% P64P (CCG→CCA PP_1208 → SlyX family protein
RA 1,419,548:1 +C 100% intergenic (‑37/+99) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein
RA 1,499,480 2 bp→AC 100% intergenic (+57/‑106) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,506:1 +C 100% intergenic (+83/‑81) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
JC 1,780,067 Δ62 bp 36.5% intergenic (+26/+19) PP_1587 → / ← dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
RA 1,838,351 C→T 6.2% A74T (GCC→ACC)  PP_1643 ← membrane protein
RA 1,886,346 T→C 7.1% L338P (CTG→CCG)  rmuC → DNA recombination limiting inversions protein
RA 1,902,235 A→T 5.6% E929V (GAG→GTG)  PP_1703 → assimilatory nitrate reductase/sulfite reductase
JC 1,930,392 Δ63 bp 24.3% [apeB] [apeB]
RA 1,932,638:1 +C 100% intergenic (‑105/+45) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
RA 2,017,246 Δ1 bp 100% intergenic (‑854/‑1480) PP_1795 ← / → PP_1797 hypothetical protein/HlyD family secretion protein
RA 2,252,389 G→T 11.5% W97L (TGG→TTG)  leuD → 3‑isopropylmalate dehydratase small subunit
JC 2,282,749 Δ66 bp 37.0% intergenic (‑141/+23) PP_2010 ← / ← PP_2011 cytochrome b561/hypothetical protein
RA 2,555,196 A→G 11.1% T393A (ACC→GCC)  PP_2240 → ABC transporter permease/ATP‑binding protein
RA 2,563,262 G→A 5.1% G334S (GGC→AGC)  dauA → FAD‑dependent catabolic D‑arginine dehydrogenase
RA 2,614,878 A→G 8.3% T1273A (ACC→GCC)  PP_2287 → internal core protein
RA 2,714,590 C→T 5.9% G172S (GGC→AGC)  metH ← cobalamin‑dependent methionine synthase
RA 2,741,599 C→T 10.2% R1326R (CGC→CGT PP_2395 → leucine‑rich repeat‑containing protein
RA 2,764,618 G→A 5.9% V128V (GTC→GTT PP_2418 ← cobalamin ABC transporter substrate‑binding protein
RA 2,863,829 T→G 40.0% S297A (TCG→GCG)  gllR → HTH‑type transcriptional regulator GalR
JC 2,957,081 Δ84 bp 43.9% intergenic (+36/‑77) PP_2588 → / → PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
RA 2,958,523 C→T 100% A428V (GCA→GTA)  PP_2589 → aldehyde dehydrogenase family protein
RA 3,227,704 G→A 6.1% Q181Q (CAG→CAA PP_2828 → hypothetical protein
RA 3,323,603 T→G 22.3% D97A (GAC→GCC)  PP_2923 ← hypothetical protein
RA 3,393,403 T→A 14.7% intergenic (‑125/+104) PP_2996 ← / ← PP_2997 hypothetical protein/protein CnmA
RA 3,447,291 T→G 22.6% A19A (GCT→GCG PP_5556 → bacteriophage lysis protein
JC 3,464,245 Δ92 bp 12.9% coding (22‑113/1929 nt) PP_3078 ← ABC transporter substrate‑binding protein
RA 3,796,112 G→A 6.5% N114N (AAC→AAT fcs ← feruloyl‑CoA‑synthetase
RA 3,845,703:1 +G 100% intergenic (‑70/+28) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
RA 3,924,436 C→G 8.0% K126N (AAG→AAC peaB ← quinohemoprotein amine dehydrogenase modification protein
RA 3,951,159 T→C 100% intergenic (‑123/+72) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,951,161 A→T 100% intergenic (‑125/+70) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,959,885 G→A 6.8% A167V (GCG→GTG)  mnxG ← manganese‑oxidizing multicopper oxidase
RA 4,001,773 T→G 36.8% intergenic (+90/‑44) dmoA‑II → / → allE dimethyl‑sulfide monooxygenase/S‑ureidoglycine aminohydrolase
RA 4,095,111 C→T 5.7% A125T (GCA→ACA)  PP_3603 ← GntR family transcriptional regulator
RA 4,146,534 T→C 7.8% F206S (TTC→TCC)  PP_3651 → two‑component system sensor histidine kinase/response regulator
RA 4,158,466 T→G 56.4% L159R (CTG→CGG)  PP_3661 → membrane protein
RA 4,158,471 A→C 29.1% S161R (AGC→CGC)  PP_3661 → membrane protein
MC JC 4,274,119 32 bp→45 bp 100% intergenic (‑243/‑1) glcC ← / → glcD transcriptional regulator GlcC/glycolate oxidase FAD‑linked subunit
RA 4,348,812 A→G 59.1% intergenic (‑70/+404) PP_3820 ← / ← galU group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
RA 4,442,726 A→C 26.9% intergenic (‑89/+49) nicP‑II ← / ← nicT porin‑like protein/metabolite transport protein NicT
RA 4,545,927 G→T 6.4% intergenic (‑15/+35) hyuC ← / ← pydP bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase/NCS1 family transporter PydP
RA 4,586,137 2 bp→TC 100% intergenic (+113/+101) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,140:1 +G 100% intergenic (+116/+99) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,163:1 +C 100% intergenic (+139/+76) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,606,576 G→A 10.8% R52H (CGC→CAC)  PP_4078 → hypothetical protein
RA 4,741,336 A→C 100% intergenic (+466/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,338 C→G 100% intergenic (+468/+285) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,341 C→T 100% intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,343 2 bp→TG 100% intergenic (+473/+279) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,346 A→G 100% intergenic (+476/+277) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,350 3 bp→TGC 100% intergenic (+480/+271) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,362 C→T 100% intergenic (+492/+261) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,364 A→C 100% intergenic (+494/+259) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,367 2 bp→GC 100% intergenic (+497/+255) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,370 A→C 100% intergenic (+500/+253) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,379 A→G 100% intergenic (+509/+244) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,381 A→C 100% intergenic (+511/+242) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,383 G→C 100% intergenic (+513/+240) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,385 C→T 100% intergenic (+515/+238) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,390:1 +T 100% intergenic (+520/+233) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,396 C→G 100% intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,399 C→T 100% intergenic (+529/+224) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,401 C→G 100% intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,403 A→C 100% intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,405 C→T 100% intergenic (+535/+218) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,754,544 C→T 6.6% A286V (GCC→GTC)  pvdR → ABC transporter permease
RA 4,767,982 A→T 100% intergenic (+89/‑93) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,767,984 T→G 100% intergenic (+91/‑91) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,768,005 T→A 100% intergenic (+112/‑70) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,850,315 G→C 29.5% intergenic (+17/‑89) PP_4263 → / → hemN cytochrome biogenesis protein/oxygen‑independent coproporphyrinogen III dehydrogenase
MC JC 4,875,480 Δ756 bp 100% coding (1‑756/756 nt) PP_4283 ← GntR family transcriptional regulator
RA 4,923,012 G→A 6.6% R573H (CGC→CAC)  PP_4330 → toxin‑like protein
JC 4,982,288 +GGC 93.2% intergenic (‑24/+44) PP_4387 ← / ← flgE hypothetical protein/flagellar hook protein FlgE
RA 5,182,361 A→C 15.3% V110G (GTG→GGG)  def‑II ← peptide deformylase
RA 5,196,590 T→G 16.1% intergenic (+29/+24) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein
JC 5,203,271 Δ98 bp 14.3% intergenic (‑54/‑29) PP_4582 ← / → PP_4583 membrane protein/putative Peptidase
RA 5,258,571 C→G 24.8% intergenic (+20/+25) PP_5703 → / ← PP_4634 type III effector protein HopJ/alpha/beta family hydrolase
RA 5,301,098 G→C 11.9% A236G (GCG→GGG)  recD ← multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease
RA 5,301,104 A→C 21.5% L234R (CTG→CGG)  recD ← multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease
RA 5,368,842 A→T 5.9% S80T (TCC→ACC)  rlmE ← ribosomal RNA large subunit methyltransferase E
RA 5,396,064 A→G 100% V231A (GTA→GCA)  tphBII ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase
RA 5,449,597 C→G 6.0% A96G (GCC→GGC)  hemL → glutamate‑1‑semialdehyde 2,1‑aminomutase
RA 5,512,711 A→C 25.9% Q32P (CAG→CCG)  urtB → urea ABC transporter permease
RA 5,512,720 T→G 23.8% L35R (CTG→CGG)  urtB → urea ABC transporter permease
JC 5,539,836 Δ81 bp 17.5% [nadE] [nadE]
JC 5,658,396 Δ80 bp 16.8% intergenic (‑36/+37) epd ← / ← tktA D‑erythrose 4‑phosphate dehydrogenase/transketolase A
RA 5,677,565:1 +G 100% intergenic (+112/+28) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,677,568 G→C 100% intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,684,230:1 +CGGG 100% intergenic (‑133/+71) PP_4986 ← / ← PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
RA 5,815,885 A→G 5.2% intergenic (+178/‑10) priA → / → argS primosome assembly protein/arginine‑‑tRNA ligase
RA 5,817,895 A→T 5.1% Y88F (TAC→TTC)  PP_5090 → cell division protein
RA 5,887,840 T→C 5.5% A38A (GCA→GCG PP_5159 ← hypothetical protein
RA 5,960,493 Δ1 bp 5.6% coding (1082/2856 nt) cyaA → adenylate cyclase
RA 6,016,700 T→C 100% intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
RA 6,124,403 C→T 6.2% V188M (GTG→ATG)  PP_5371 ← rubredoxin/rubredoxin reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947_CJ‑RC 171372–172962 176294–172973 12–4923 5 [4] [4] 5 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947_CJ‑RC 176619–178392 181772–178403 12–5154 5 [4] [4] 5 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947_CJ‑RC 2194907 2195063 157 5 [4] [4] 5 PP_1940 methyl‑accepting chemotaxis transducer
* * ÷ NC_002947_CJ‑RC 2478365 2478460 96 5 [3] [4] 5 PP_2172 hypothetical protein
* * ÷ NC_002947_CJ‑RC 2820736 2821505 770 5 [4] [4] 5 [PP_2474] [PP_2474]
* * ÷ NC_002947_CJ‑RC 3776349 3776398 50 5 [4] [4] 5 PP_3338/PP_3339 cytochrome bd‑type quinol oxidase/hypothetical protein
* * ÷ NC_002947_CJ‑RC 4225976 4226301 326 5 [4] [4] 5 [PP_3705]–[PP_5623] [PP_3705],[PP_5623]
* * ÷ NC_002947_CJ‑RC 4621501 4621640 140 5 [4] [4] 5 [PP_5654] [PP_5654]
* * ÷ NC_002947_CJ‑RC 4630526 4630659 134 6 [4] [4] 5 PP_4094/PP_4096 hypothetical protein/hypothetical protein
* * ÷ NC_002947_CJ‑RC 5509352 5509410 59 5 [4] [4] 5 [PP_4839] [PP_4839]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 247211515 (0.320)4 (0.090) 4/256 NT 16.3% intergenic (+19/+130) PP_2166/PP_2167 anti‑anti‑sigma factor/PhoD‑like phosphatase
?NC_002947_CJ‑RC 2472313 = 27 (0.610)coding (1783/1851 nt) PP_2167 PhoD‑like phosphatase
* ? NC_002947_CJ‑RC 5046766 =9 (0.190)33 (0.710) 26/268 NT 78.7% coding (136/237 nt) PP_5681 hypothetical protein
?NC_002947_CJ‑RC = 5406601 NA (NA)intergenic (‑84/+633) PP_4746/PP_4748 transposase/amino acid ABC transporter substrate‑binding protein
* ? NC_002947_CJ‑RC = 504677412 (0.260)7 (0.150) 7/270 NT 36.8% coding (128/237 nt) PP_5681 hypothetical protein
?NC_002947_CJ‑RC 5404562 = NA (NA)intergenic (+1123/+45) PP_4743/PP_4745 hypothetical protein/transposase
* ? NC_002947_CJ‑RC 5140764 =103 (2.190)4 (0.090) 3/266 NT 5.9% coding (153/1380 nt) PP_4524 sodium‑solute symporter
?NC_002947_CJ‑RC = 6168725 26 (0.560)coding (332/963 nt) PP_5406 transposase
* ? NC_002947_CJ‑RC 5306595 =105 (2.230)5 (0.110) 5/250 NT 5.1% coding (2360/3483 nt) recC multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit
?NC_002947_CJ‑RC 5306636 = 89 (2.040)coding (2319/3483 nt) recC multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit
* ? NC_002947_CJ‑RC = 5406601NA (NA)4 (0.090) 4/268 NT 13.0% intergenic (‑84/+633) PP_4746/PP_4748 transposase/amino acid ABC transporter substrate‑binding protein
?NC_002947_CJ‑RC 6168718 = 27 (0.570)coding (339/963 nt) PP_5406 transposase
* ? NC_002947_CJ‑RC = 599158614 (0.300)6 (0.140) 6/254 NT 29.9% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991778 = 15 (0.340)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA