breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 68,532 T→C 5.7% G247G (GGA→GGG PP_0058 ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
RA 117,271 A→T 28.5% intergenic (+51/+43) PP_0111 → / ← metQ electron transport protein SCO1/SenC/methionine ABC transporter substrate‑binding protein
RA 152,348 C→T 6.4% G420D (GGC→GAC)  PP_0144 ← insulinase family metalloprotease
RA 181,805 T→C 5.5% intergenic (+19/‑87) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,811 C→T 5.2% intergenic (+25/‑81) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,812 A→G 5.2% intergenic (+26/‑80) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,818 G→A 5.3% intergenic (+32/‑74) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 182,035 A→T 8.7% intergenic (+24/+41) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor
RA 182,036 T→C 9.5% intergenic (+25/+40) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor
RA 195,559 C→A 25.2% T355T (ACC→ACA PP_0168 → putative surface adhesion protein
RA 195,628 C→T 9.6% D378D (GAC→GAT PP_0168 → putative surface adhesion protein
RA 195,629 A→G 9.7% T379A (ACC→GCC)  PP_0168 → putative surface adhesion protein
RA 195,634 C→T 40.8% T380T (ACC→ACT PP_0168 → putative surface adhesion protein
RA 196,306 T→C 25.0% N604N (AAT→AAC PP_0168 → putative surface adhesion protein
RA 196,327 A→C 26.7% T611T (ACA→ACC PP_0168 → putative surface adhesion protein
RA 196,351 C→G 29.2% T619T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 196,390 T→C 31.7% D632D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 197,089 T→C 13.5% N865N (AAT→AAC PP_0168 → putative surface adhesion protein
RA 197,551 C→G 11.7% T1019T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 197,572 G→A 20.0% K1026K (AAG→AAA PP_0168 → putative surface adhesion protein
RA 197,590 T→C 21.8% D1032D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 197,632 T→C 15.6% T1046T (ACT→ACC PP_0168 → putative surface adhesion protein
RA 197,695 G→C 15.6% T1067T (ACG→ACC PP_0168 → putative surface adhesion protein
RA 278,742:1 +A 100% intergenic (‑237/+77) PP_0224 ← / ← sctC DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
RA 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein
RA 336,124:1 +T 100% coding (209/237 nt) PP_0278 → hypothetical protein
RA 346,486 G→A 6.0% R454C (CGC→TGC)  PP_0287 ← hypothetical protein
RA 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
RA 441,313 A→C 22.4% E65A (GAG→GCG)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 499,203 A→G 100% intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
RA 549,164 C→T 100% L672F (CTC→TTC)  fusA → elongation factor G 1
RA 556,678 G→A 6.3% D94N (GAC→AAC)  rplE → 50S ribosomal protein L5
RA 568,446 G→A 5.5% S350S (AGC→AGT uvrA ← uvrABC excinuclease protein A
RA 578,118 C→T 5.9% I29I (ATC→ATT fdoH → formate dehydrogenase‑O subunit beta
RA 653,752 C→T 5.8% G331R (GGA→AGA)  ctpL ← methyl‑accepting chemotaxis protein CtpL
RA 669,264 T→A 7.1% W236R (TGG→AGG)  pbpC → peptidoglycan glycosyltransferase
RA 699,722 A→G 51.9% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA
RA 759,811 A→G 5.8% intergenic (+123/‑51) PP_mr12 → / → PP_5453 C4 ncRNA/hypothetical protein
RA 777,159 T→C 6.3% L70P (CTG→CCG)  PP_0669 → ferric siderophore receptor
RA 811,064 G→C 5.4% V82V (GTG→GTC PP_5455 → hypothetical protein
RA 837,327 A→G 7.1% L10P (CTG→CCG)  ychF ← ribosome‑associated potassium‑dependent ATP/GTPase
RA 1,032,274 T→A 8.2% F123Y (TTC→TAC)  PP_0889 → hypothetical protein
RA 1,126,645:1 +C 100% intergenic (‑99/+50) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase
RA 1,155,012 G→A 8.0% S57N (AGC→AAC)  PP_1013 → sensor histidine kinase
RA 1,327,121 C→T 55.9% intergenic (+97/‑196) PP_16SE → / → PP_23SE 16S ribosomal RNA/23S ribosomal RNA
RA 1,419,548:1 +C 100% intergenic (‑37/+99) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein
RA 1,429,310 C→T 7.3% P471S (CCG→TCG)  mqo‑II → malate:quinone oxidoreductase
RA 1,456,328 C→A 5.7% A219S (GCC→TCC)  PP_1274 ← short‑chain dehydrogenase/reductase family oxidoreductase
RA 1,474,995 T→G 9.5% intergenic (‑775/+26) algD ← / ← PP_1289 GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein
RA 1,499,480 2 bp→AC 100% intergenic (+57/‑106) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,498 1 bp→CG 100% intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,506:1 +C 100% intergenic (+83/‑81) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,536,064 A→G 7.0% Y358C (TAT→TGT)  argJ → glutamate N‑acetyltransferase/amino acid acetyltransferase
RA 1,649,745 Δ1 bp 7.1% coding (1315/2043 nt) PP_1446 ← TonB‑dependent receptor
RA 1,671,666 G→A 6.0% A408V (GCC→GTC)  PP_1467 ← NhaP‑type Na+(K+)/H+ antiporter
RA 1,691,786 T→G 9.7% intergenic (+53/‑118) PP_1487 → / → PP_1488 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 1,691,796 C→G 6.8% intergenic (+63/‑108) PP_1487 → / → PP_1488 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 1,702,572 C→T 5.8% Y308Y (TAC→TAT lysS → lysine‑‑tRNA ligase
RA 1,732,209 A→G 6.7% F554S (TTC→TCC)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase
RA 1,747,351 T→G 27.6% E53A (GAG→GCG)  PP_5473 ← LuxR family transcriptional regulator
JC 1,780,067 Δ62 bp 63.7% intergenic (+26/+19) PP_1587 → / ← dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
RA 1,905,088 G→A 6.9% S448N (AGC→AAC)  nirB → nitrite reductase large subunit
RA 1,926,122 A→T 7.8% R248R (CGT→CGA PP_1726 ← ABC transporter substrate‑binding protein
RA 1,932,638:1 +C 100% intergenic (‑105/+45) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
RA 1,960,112 C→G 13.1% intergenic (+63/‑65) bolA → / → PP_1758 DNA‑binding transcriptional dual regulator/rhodanese‑like domain‑containing sulfurtransferase
RA 1,993,604 A→C 14.0% intergenic (+42/+56) PP_1780 → / ← PP_1781 mannosyltransferase/acyltransferase
RA 2,100,946 T→C 5.8% Y285H (TAC→CAC)  PP_1876 → AAA family ATP/GTP‑binding protein
RA 2,101,751 A→C 24.6% L186R (CTG→CGG)  PP_1878 ← hypothetical protein
RA 2,162,508 A→G 6.9% intergenic (+190/‑632) yceG → / → PP_5482 septation protein/hypothetical protein
RA 2,200,607 G→A 6.3% V61M (GTG→ATG)  folD‑I → bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 2,278,653 G→C 5.7% intergenic (‑74/‑208) PP_2007 ← / → fadH P‑47‑like protein/2,4‑dienoyl‑CoA reductase
JC 2,282,749 Δ66 bp 23.1% intergenic (‑141/+23) PP_2010 ← / ← PP_2011 cytochrome b561/hypothetical protein
RA 2,291,752 T→C 5.5% I49I (ATT→ATC PP_2019 → RND family multidrug transporter
RA 2,300,593 A→G 5.5% I255T (ATC→ACC)  sbcD ← exonuclease subunit SbcD
RA 2,300,653 A→G 5.5% V235A (GTC→GCC)  sbcD ← exonuclease subunit SbcD
RA 2,355,186 G→T 9.0% A275A (GCC→GCA PP_2069 ← multidrug MFS transporter outer membrane protein
RA 2,535,685 T→C 8.7% K33R (AAG→AGG)  mrpD ← K(+)/H(+) antiporter subunit D
RA 2,720,828 G→A 6.6% L71F (CTC→TTC)  PP_2381 ← hypothetical protein
RA 2,793,755 A→C 17.0% intergenic (+64/+63) PP_2445 → / ← PP_2446 membrane protein/hypothetical protein
RA 2,823,440 T→A 7.8% V338E (GTG→GAG)  curA → NADPH‑dependent curcumin/dihydrocurcumin reductase
RA 2,883,429 C→T 5.9% intergenic (+115/‑12) PP_2538 → / → PP_2539 hypothetical protein/LysR family transcriptional regulator
RA 2,892,757 G→A 7.3% S4F (TCC→TTC)  PP_2547 ← LysR family transcriptional regulator
RA 2,951,341 A→C 11.5% intergenic (‑120/+41) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
RA 2,951,342 G→T 17.5% intergenic (‑121/+40) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
JC 2,957,081 Δ84 bp 24.7% intergenic (+36/‑77) PP_2588 → / → PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
RA 2,958,523 C→T 100% A428V (GCA→GTA)  PP_2589 → aldehyde dehydrogenase family protein
RA 3,026,055 A→G 7.5% V194A (GTC→GCC)  PP_2641 ← iron‑sulfur cluster‑binding protein
RA 3,128,355 A→G 6.0% L21P (CTG→CCG)  PP_2745 ← universal stress protein family protein
RA 3,171,774 A→T 17.5% I16F (ATT→TTT)  PP_2784 → short‑chain dehydrogenase/reductase family oxidoreductase
RA 3,447,291 T→G 29.3% A19A (GCT→GCG PP_5556 → bacteriophage lysis protein
RA 3,492,271 C→T 7.4% E50K (GAA→AAA)  PP_3096 ← hypothetical protein
RA 3,574,093 A→G 6.2% W418R (TGG→CGG)  PP_3155 ← ferric siderophore receptor
RA 3,612,428 C→T 6.0% G419D (GGC→GAC)  mcoA ← Mn(II) copper oxidase A
RA 3,653,438 G→A 11.4% intergenic (+4/+94) PP_3218 → / ← PP_3219 NtaA/SnaA/SoxA family monooxygenase/alkansulfonate monooxygenase
RA 3,714,009 C→A 6.7% G169C (GGC→TGC)  paaK ← phenylacetate‑CoA ligase
RA 3,721,979 C→T 6.7% P75P (CCG→CCA fnrC ← Crp/Fnr family transcriptional regulator
RA 3,791,727 T→G 36.9% S531R (AGC→CGC)  PP_3354 ← acyl‑CoA dehydrogenase
RA 3,810,630 A→G 8.1% P249P (CCA→CCG PP_3366 → LysR family transcriptional regulator
RA 3,845,703:1 +G 100% intergenic (‑70/+28) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
RA 3,883,600 G→A 7.1% G207S (GGC→AGC)  PP_3428 → TPR repeat‑containing protein
RA 3,891,788 G→T 7.0% A258S (GCC→TCC)  PP_3435 → hypothetical protein
RA 3,951,159 T→C 100% intergenic (‑123/+72) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,951,161 A→T 100% intergenic (‑125/+70) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,996,335 T→G 26.6% intergenic (+13/+32) PP_3523 → / ← PP_3524 hypothetical protein/hypothetical protein
RA 4,110,767 C→T 6.9% Y863Y (TAC→TAT PP_3616 → membrane protein
RA 4,158,466 T→G 47.8% L159R (CTG→CGG)  PP_3661 → membrane protein
RA 4,158,471 A→C 36.1% S161R (AGC→CGC)  PP_3661 → membrane protein
MC JC 4,274,119 32 bp→45 bp 100% intergenic (‑243/‑1) glcC ← / → glcD transcriptional regulator GlcC/glycolate oxidase FAD‑linked subunit
RA 4,322,176 A→G 6.1% intergenic (‑79/‑1344) PP_3792 ← / → PP_5631 hypothetical protein/hypothetical protein
RA 4,348,812 A→G 48.8% intergenic (‑70/+404) PP_3820 ← / ← galU group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
RA 4,373,575 A→C 5.9% N161T (AAC→ACC)  PP_3849 → hemolysin‑type calcium‑binding protein
RA 4,436,996 T→A 7.0% Y17N (TAT→AAT)  PP_3932 → diguanylate cyclase
RA 4,442,726 A→C 24.1% intergenic (‑89/+49) nicP‑II ← / ← nicT porin‑like protein/metabolite transport protein NicT
RA 4,556,095 T→G 7.6% E170A (GAG→GCG)  zwfB ← glucose 6‑phosphate 1‑dehydrogenase
RA 4,585,353 C→T 5.8% C113Y (TGC→TAC)  glgE ← alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
RA 4,586,137 2 bp→TC 100% intergenic (+113/+101) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,140:1 +G 100% intergenic (+116/+99) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,163:1 +C 100% intergenic (+139/+76) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,598,251 C→T 6.6% L555L (CTG→CTA PP_4071 ← membrane protein
RA 4,714,982 C→G 43.1% R36G (CGC→GGC)  PP_4173 → two‑component system sensor histidine kinase/response regulator
RA 4,740,912 Δ1 bp 100% intergenic (+42/+711) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,923 T→G 100% intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,926 2 bp→CT 100% intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,336 A→C 100% intergenic (+466/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,338 C→G 100% intergenic (+468/+285) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,341 C→T 100% intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,343 2 bp→TG 100% intergenic (+473/+279) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,346 A→G 100% intergenic (+476/+277) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,350 3 bp→TGC 100% intergenic (+480/+271) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,362 C→T 100% intergenic (+492/+261) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,364 A→C 100% intergenic (+494/+259) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,367 2 bp→GC 100% intergenic (+497/+255) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,370 A→C 100% intergenic (+500/+253) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,379 A→G 100% intergenic (+509/+244) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,381 A→C 100% intergenic (+511/+242) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,383 G→C 100% intergenic (+513/+240) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,385 C→T 100% intergenic (+515/+238) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,390:1 +T 100% intergenic (+520/+233) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,396 C→G 100% intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,399 C→T 100% intergenic (+529/+224) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,401 C→G 100% intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,403 A→C 100% intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,405 C→T 100% intergenic (+535/+218) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,745,946 G→A 6.1% N56N (AAC→AAT PP_4200 ← hypothetical protein
RA 4,767,982 A→T 100% intergenic (+89/‑93) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,767,984 T→G 100% intergenic (+91/‑91) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,768,005 T→A 100% intergenic (+112/‑70) fpvA → / → tpaK TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
RA 4,768,243 C→A 59.9% L57M (CTG→ATG)  tpaK → probable terephthalate transporter, MFS superfamily protein
RA 4,769,141 A→T 44.4% N356I (AAT→ATT)  tpaK → probable terephthalate transporter, MFS superfamily protein
RA 4,795,178 G→A 7.0% A122V (GCG→GTG)  pvdI ← non‑ribosomal peptide synthetase
RA 4,798,307 A→G 15.9% intergenic (‑162/+56) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
RA 4,798,315 C→T 18.5% intergenic (‑170/+48) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
RA 4,798,317 G→C 29.0% intergenic (‑172/+46) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
RA 4,798,320 G→C 20.8% intergenic (‑175/+43) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
RA 4,798,322 A→G 25.7% intergenic (‑177/+41) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
RA 4,798,330 C→T 19.0% intergenic (‑185/+33) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
MC JC 4,875,480 Δ756 bp 100% coding (1‑756/756 nt) PP_4283 ← GntR family transcriptional regulator
RA 4,888,972 C→T 7.7% H110Y (CAC→TAC)  gcl → glyoxylate carboligase
RA 4,948,753 C→T 6.1% A104T (GCG→ACG)  fliR ← flagellar biosynthetic protein FliR
RA 4,975,791 C→T 6.5% A506T (GCC→ACC)  flgK ← flagellar hook‑associated protein FlgK
JC 4,982,288 +GGC 93.2% intergenic (‑24/+44) PP_4387 ← / ← flgE hypothetical protein/flagellar hook protein FlgE
RA 5,111,161 A→G 6.4% Y416Y (TAT→TAC aroP‑II ← aromatic amino acid transport protein
RA 5,123,530 G→C 18.2% A308G (GCG→GGG)  PP_4508 ← AraC family transcriptional regulator
RA 5,123,537 G→C 11.1% L306V (CTG→GTG)  PP_4508 ← AraC family transcriptional regulator
RA 5,140,117 G→C 6.6% D201H (GAC→CAC)  PP_4523 → agmatinase
RA 5,144,356 A→G 7.3% T330A (ACC→GCC)  PP_4526 → MFS transporter
RA 5,191,670 T→A 7.6% N100I (AAC→ATC)  PP_4570 ← hypothetical protein
RA 5,218,255 G→A 7.3% P52S (CCC→TCC)  PP_4596 ← putative oxidoreductase
RA 5,255,511 C→T 6.5% G276S (GGC→AGC)  PP_4630 ← MerR family transcriptional regulator
RA 5,279,700 G→A 6.3% A320T (GCA→ACA)  PP_4652 → membrane protein
RA 5,373,996 C→T 6.2% A293T (GCC→ACC)  carA ← carbamoyl‑phosphate synthase small subunit
RA 5,396,064 A→G 100% V231A (GTA→GCA)  tphBII ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase
RA 5,462,375 T→G 43.4% intergenic (+18/‑22) PP_4795 → / → holA lipoprotein/DNA polymerase III subunit delta
RA 5,464,465 C→T 9.7% A197V (GCC→GTC)  PP_4798 → membrane‑bound lytic murein transglycosylase
RA 5,484,356 C→T 7.0% P344S (CCT→TCT)  PP_4819 → hypothetical protein
RA 5,491,140 G→A 5.1% A552T (GCC→ACC)  PP_4824 → two‑component system sensor histidine kinase/response regulator
JC 5,558,423 +GCC 94.8% intergenic (‑84/+87) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,558,463 T→A 20.7% intergenic (‑124/+47) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,558,464 A→T 15.4% intergenic (‑125/+46) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,558,465 T→A 13.0% intergenic (‑126/+45) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,579,540 G→A 6.5% L149L (CTG→CTA PP_4906 → hypothetical protein
RA 5,589,364 A→G 8.0% L246P (CTG→CCG)  PP_4914 ← phytase‑like protein
RA 5,668,822 G→A 5.3% A7A (GCG→GCA nhaP → NhaP‑type Na+(K+)/H+ antiporter
RA 5,677,565:1 +G 100% intergenic (+112/+28) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,677,568 G→C 100% intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,678,475 T→C 5.7% H94R (CAC→CGC)  yceJ ← iron‑regulated cytochrome b561
JC 5,684,230 +CGGG 91.9% intergenic (‑133/+71) PP_4986 ← / ← PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
RA 5,778,383 G→A 6.6% V479I (GTC→ATC)  betB → NAD‑dependent betaine aldehyde dehydrogenase
RA 5,911,923 G→T 5.2% A38D (GCT→GAT)  spuD ← spermidine/putrescine ABC transporter substrate‑binding protein
RA 5,985,764 G→T 5.1% L1198L (CTG→CTT PP_5242 → GAF domain‑containing sensor histidine kinase
RA 5,991,634 T→C 16.0% intergenic (+113/‑433) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,639 C→T 16.3% intergenic (+118/‑428) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,640 G→T 18.5% intergenic (+119/‑427) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,641 C→T 16.3% intergenic (+120/‑426) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,644 A→G 16.3% intergenic (+123/‑423) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,650 G→C 16.7% intergenic (+129/‑417) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,652 C→G 14.1% intergenic (+131/‑415) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,659 C→G 15.9% intergenic (+138/‑408) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,663 C→T 15.9% intergenic (+142/‑404) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,665 C→T 15.5% intergenic (+144/‑402) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,709 C→T 10.2% intergenic (+188/‑358) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,710 A→G 10.1% intergenic (+189/‑357) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,720 T→C 13.1% intergenic (+199/‑347) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,991,726 Δ1 bp 12.5% intergenic (+205/‑341) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 6,016,700 T→C 100% intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
RA 6,093,871 A→G 5.6% L144S (TTG→TCG)  PP_5342 ← GntR family transcriptional regulator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947_CJ‑RC 171367–176306 176306 1–4940 25 [23] [23] 25 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947_CJ‑RC 176643–181695 181695 1–5053 24 [23] [21] 24 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947_CJ‑RC 2837045 2837286 242 24 [23] [23] 24 [sad‑I]–[PP_2489] [sad‑I],[PP_2489]
* * ÷ NC_002947_CJ‑RC 5509258 5509446 189 24 [23] [23] 24 [PP_4839] [PP_4839]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 65278237 (0.670)3 (0.060) 3/232 NT 16.0% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947_CJ‑RC = 4809862 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947_CJ‑RC 3366553 =42 (0.760)3 (0.050) 3/272 NT 5.7% intergenic (+219/‑142) PP_2962/PP_2964 alcohol dehydrogenase/transposase
?NC_002947_CJ‑RC = 5399071 58 (1.050)coding (482/1218 nt) hsdM type I restriction modification system methylase
* ? NC_002947_CJ‑RC 4046130 =36 (0.650)3 (0.060) 3/258 NT 8.3% coding (590/906 nt) PP_3567 LysR family transcriptional regulator
?NC_002947_CJ‑RC 4046163 = 32 (0.610)coding (557/906 nt) PP_3567 LysR family transcriptional regulator
* ? NC_002947_CJ‑RC = 45861368 (0.140)15 (0.290) 11/250 NT 59.6% intergenic (+112/+103) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947_CJ‑RC = 4586216 13 (0.260)intergenic (+192/+23) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947_CJ‑RC 4809877 =0 (0.000)7 (0.170) 6/202 NT 37.8% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC = 5991750 23 (0.560)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 48098820 (0.000)8 (0.160) 7/248 NT 28.6% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947_CJ‑RC 5991580 = 40 (0.790)intergenic (+59/‑487) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC 5194427 =22 (0.400)5 (0.110) 4/222 NT 29.4% intergenic (+105/‑97) PP_4572/PP_4573 hypothetical protein/AAA family ATPase
?NC_002947_CJ‑RC 5194496 = 6 (0.130)intergenic (+174/‑28) PP_4572/PP_4573 hypothetical protein/AAA family ATPase
* ? NC_002947_CJ‑RC = 519765758 (1.050)3 (0.060) 3/254 NT 5.3% coding (561/690 nt) ybgJ allophanate hydrolase subunit 1
?NC_002947_CJ‑RC = 5197813 52 (1.010)coding (405/690 nt) ybgJ allophanate hydrolase subunit 1
* ? NC_002947_CJ‑RC 5655737 =27 (0.490)4 (0.080) 4/258 NT 18.3% intergenic (‑23/+138) PP_4961/PP_4962 lipoprotein/hypothetical protein
?NC_002947_CJ‑RC 5655825 = 10 (0.190)intergenic (‑111/+50) PP_4961/PP_4962 lipoprotein/hypothetical protein
* ? NC_002947_CJ‑RC = 599157440 (0.720)5 (0.100) 5/258 NT 13.5% intergenic (+53/‑493) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991766 = 26 (0.500)intergenic (+245/‑301) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947_CJ‑RC = 599158638 (0.690)4 (0.080) 4/256 NT 11.1% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947_CJ‑RC 5991778 = 28 (0.540)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA