BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	4,700,807	Δ1 bp	intergenic (+165/‑150)	PA14_RS21585 → / → pbpG	aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	4,700,807	0	G	.	100.0%	2539.4 / NA	511	intergenic (+165/‑150)	PA14_RS21585/pbpG	aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (200/311); total (200/311)

Only 100 of 511 total aligned reads displayed.

GGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAA  >  NC_008463/4700665‑4700951

  cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCtctc                                                                                                                                        >  1:9314095/1‑150 (MQ=255)

     tgACGGCCGGCTTTTTATCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGa                                                                                                                                     <  1:2819278/150‑1 (MQ=255)

       aTGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAc                                                                                                                                   <  1:9382565/150‑1 (MQ=255)

       aTGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAc                                                                                                                                   <  1:7298852/150‑1 (MQ=255)

        tGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAcc                                                                                                                                  <  2:3119793/150‑1 (MQ=255)

        tGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAcc                                                                                                                                  <  2:596806/150‑1 (MQ=255)

         ggCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGACCCTCTCCGAACCg                                                                                                                                 <  1:8262941/150‑1 (MQ=255)

           ccGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTg                                                                                                                               >  2:5009118/1‑150 (MQ=255)

              gCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCGCCGAACCGTGCCa                                                                                                                            >  2:7545231/1‑150 (MQ=255)

                tttttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAAc                                                                                                                          <  1:883434/150‑1 (MQ=255)

                tttttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAAc                                                                                                                          >  1:2938399/1‑150 (MQ=255)

                tttttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAAc                                                                                                                          >  2:9049694/1‑150 (MQ=255)

                  tttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCa                                                                                                                        <  1:3874841/150‑1 (MQ=255)

                   ttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCAt                                                                                                                       >  1:9758826/1‑150 (MQ=255)

                   ttttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCAAACCAt                                                                                                                       >  1:2700763/1‑150 (MQ=255)

                     ttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATaa                                                                                                                     >  2:233491/1‑150 (MQ=255)

                       cGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGt                                                                                                                   <  1:6822472/150‑1 (MQ=255)

                       cGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGt                                                                                                                   <  2:8061806/150‑1 (MQ=255)

                         ccccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCCACCATAAGTCa                                                                                                                 >  1:3177610/1‑150 (MQ=255)

                          cccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAg                                                                                                                >  1:4849612/1‑150 (MQ=255)

                              gCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACt                                                                                                            <  2:5452605/150‑1 (MQ=255)

                               cGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACtt                                                                                                           <  2:7980130/150‑1 (MQ=255)

                               cGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACtt                                                                                                           <  2:10534995/150‑1 (MQ=255)

                                gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACttt                                                                                                          <  1:2507780/150‑1 (MQ=255)

                                gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACttt                                                                                                          <  1:10007029/150‑1 (MQ=255)

                                gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACttt                                                                                                          <  1:5582417/150‑1 (MQ=255)

                                 aaTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                         <  2:9288782/150‑1 (MQ=255)

                                 aaTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                         <  2:286900/150‑1 (MQ=255)

                                  aTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCa                                                                                                        <  2:10126949/150‑1 (MQ=255)

                                    gCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCaaa                                                                                                      >  2:6310512/1‑150 (MQ=255)

                                     ccGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAg                                                                                                     <  2:6916315/150‑1 (MQ=255)

                                      cgcgCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGc                                                                                                    <  2:4882492/150‑1 (MQ=255)

                                       gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGcc                                                                                                   <  1:8524480/150‑1 (MQ=255)

                                       gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTGGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGcc                                                                                                   >  2:4322111/1‑150 (MQ=255)

                                        cgcACTCCCGCCGAGGCGGCGGATACCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGccc                                                                                                  <  1:7837694/150‑1 (MQ=255)

                                         gcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCt                                                                                                 <  1:2607029/150‑1 (MQ=255)

                                          cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                <  1:1384457/150‑1 (MQ=255)

                                            cTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACg                                                                                              >  1:6854042/1‑150 (MQ=255)

                                              cccGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                            >  2:1308989/1‑150 (MQ=255)

                                               ccgccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGc                                                                                           >  1:11031091/1‑150 (MQ=255)

                                                  ccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCttt                                                                                        >  2:9427912/1‑150 (MQ=255)

                                                   cgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                       >  1:6021713/1‑150 (MQ=255)

                                                      ggcggcGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTTGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTGTTCCt                                                                                    >  2:953337/1‑150 (MQ=255)

                                                          gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaa                                                                                >  2:3278861/1‑150 (MQ=255)

                                                          gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaa                                                                                <  2:1124611/150‑1 (MQ=255)

                                                           cggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaaa                                                                               <  1:1211635/150‑1 (MQ=255)

                                                             gATAACCGCGTTGCTGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACAGTGCCAACCATAAGTCAGCACTTACAAAGCCCTACGTGCATTTCCTACAAAag                                                                             <  1:1764463/150‑1 (MQ=255)

                                                             gATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACATAag                                                                             <  2:2585585/150‑1 (MQ=255)

                                                             gATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAag                                                                             <  1:74067/150‑1 (MQ=255)

                                                               tAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAagag                                                                           >  1:2092742/1‑150 (MQ=255)

                                                                aaCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                          <  1:4437868/150‑1 (MQ=255)

                                                                aaCCGCGTTGCGGTTAGTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                          <  2:5717896/150‑1 (MQ=255)

                                                                 aCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCa                                                                         >  2:3933322/1‑150 (MQ=255)

                                                                   cgcgTTGCGGTTATTCGCCCTACCGCACCACGTCCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCTGCACTTTCAAAGCCATACGTGCTTGTCCTGCAAAAGAGccga                                                                       >  2:7080286/1‑147 (MQ=255)

                                                                     cgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaaa                                                                     <  2:2032025/150‑1 (MQ=255)

                                                                      gTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaaaa                                                                    >  1:3450815/1‑150 (MQ=255)

                                                                       ttGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                   <  1:3754878/150‑1 (MQ=255)

                                                                       ttGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                   <  2:8291913/150‑1 (MQ=255)

                                                                       ttGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                   >  1:5234880/1‑150 (MQ=255)

                                                                        tGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATCAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATg                                                                  <  1:9992326/150‑1 (MQ=255)

                                                                        tGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGAGTTATCCGC‑CGAAACTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATg                                                                  <  1:3611079/150‑1 (MQ=255)

                                                                          cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                <  2:8579027/150‑1 (MQ=255)

                                                                           ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                               <  1:10744213/150‑1 (MQ=255)

                                                                            gTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCa                                                              >  2:8983701/1‑150 (MQ=255)

                                                                              tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAt                                                            <  2:1510767/150‑1 (MQ=255)

                                                                               aTTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAtt                                                           <  1:33303/150‑1 (MQ=255)

                                                                                ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTa                                                          <  1:6592595/150‑1 (MQ=255)

                                                                                ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCAGAAAATGCTCAAtga                                                          >  2:3642720/1‑148 (MQ=255)

                                                                                  cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                        <  1:1912263/150‑1 (MQ=255)

                                                                                   gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATgg                                                       >  1:9080628/1‑150 (MQ=255)

                                                                                    cccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATggg                                                      <  1:10625684/150‑1 (MQ=255)

                                                                                     ccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGc                                                     >  1:10160895/1‑150 (MQ=255)

                                                                                     ccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGc                                                     <  1:6241234/150‑1 (MQ=255)

                                                                                       tACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAc                                                   <  2:5628609/150‑1 (MQ=255)

                                                                                        aCCGCACCCCGACCGTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAcc                                                  <  1:2401822/150‑1 (MQ=255)

                                                                                        aCCGCACCACGCCCTTGTGGGCGTAGGGCGCATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAACGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCAcc                                                  <  2:10845266/150‑1 (MQ=255)

                                                                                            caccacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGtt                                              >  1:4093498/1‑150 (MQ=255)

                                                                                             accacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                             >  1:4267708/1‑150 (MQ=255)

                                                                                             accacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTc                                             >  1:7446608/1‑150 (MQ=255)

                                                                                               cacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcac                                           <  2:4542296/150‑1 (MQ=255)

                                                                                               cacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcac                                           >  2:5184591/1‑150 (MQ=255)

                                                                                                 cGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCacac                                         <  2:4762942/150‑1 (MQ=255)

                                                                                                      ttGTGGGCGTAGGGCGAATACCGCCACAGGCGTTAGCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCACCGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACGgcgc                                    <  1:5712158/150‑1 (MQ=255)

                                                                                                       tgtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAGAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcc                                   <  1:7028164/150‑1 (MQ=255)

                                                                                                       tgtgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcc                                   >  1:5451253/1‑150 (MQ=255)

                                                                                                               tAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcc                           >  1:9445047/1‑150 (MQ=255)

                                                                                                                aGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGccc                          >  2:8157192/1‑150 (MQ=255)

                                                                                                                aGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGccc                          <  1:7902001/150‑1 (MQ=255)

                                                                                                                 gggCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATACAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcccc                         >  2:4158731/1‑150 (MQ=255)

                                                                                                                    cGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCACACGGCCCCCAc                      >  2:1712704/1‑150 (MQ=255)

                                                                                                                      aaTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                    <  2:7767224/150‑1 (MQ=255)

                                                                                                                       aTACCGCCACCGGCGTGAACCGC‑CGAACCTCTCCGAACCCTGCCAACCATAAGACAGCACTTGCAACGCCCTACGTGCTTTTCCTACAAAAGAGCATAAACTGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCCCttt                   <  2:7381025/150‑1 (MQ=255)

                                                                                                                          ccGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTtctc                >  1:1520758/1‑150 (MQ=255)

                                                                                                                            gCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGt              >  2:2452846/1‑150 (MQ=255)

                                                                                                                              cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCt            <  2:2162037/150‑1 (MQ=255)

                                                                                                                              cacaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCt            >  1:4009634/1‑150 (MQ=255)

                                                                                                                                caGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATGATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTa          <  2:6691158/150‑1 (MQ=255)

                                                                                                                                 aGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAg         <  1:2786794/150‑1 (MQ=255)

                                                                                                                                      ttATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGc    >  2:9685797/1‑150 (MQ=255)

                                                                                                                                       tATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCa   >  1:10454911/1‑150 (MQ=255)

GGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAA  >  NC_008463/4700665‑4700951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`